<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hasan, Syed Saif</style></author><author><style face="normal" font="default" size="100%">Singh, Sanjay</style></author><author><style face="normal" font="default" size="100%">Parikh, Rasesh Y.</style></author><author><style face="normal" font="default" size="100%">Dharne, Mahesh S.</style></author><author><style face="normal" font="default" size="100%">Patole, Milind S.</style></author><author><style face="normal" font="default" size="100%">Bhagavatula L. V. Prasad</style></author><author><style face="normal" font="default" size="100%">Shouche, Yogesh S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Bacterial synthesis of copper/copper oxide nanoparticles</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Nanoscience and Nanotechnology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bacterial synthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Nanoparticles</style></keyword><keyword><style  face="normal" font="default" size="100%">TEM</style></keyword><keyword><style  face="normal" font="default" size="100%">XPS</style></keyword><keyword><style  face="normal" font="default" size="100%">XRD</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JUN</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">6</style></number><publisher><style face="normal" font="default" size="100%">AMER SCIENTIFIC PUBLISHERS</style></publisher><pub-location><style face="normal" font="default" size="100%">26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA</style></pub-location><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">3191-3196</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A bacterial mediated synthesis of copper/copper oxide nanoparticle composite is reported. A Gram-negative bacterium belonging to the genus Serratia was isolated from the midgut of Stibara sp., an insect of the Cerambycidae family of beetles found in the Northwestern Ghats of India. This is a unique bacterium that is quite specific for the synthesis of copper oxide nanoparticles as several other strains isolated from the same insect and common Indian mosquitoes did not result in nanoparticle formation. By following the reaction systematically, we could delineate that the nanoparticle formation occurs intracellularly. However, the process results in the killing of bacterial cells. Subsequently the nanoparticles leak out as the cell wall disintegrates. The nanoparticles formed are thoroughly characterized by UV-Vis, TEM, XRD, XPS and FTIR studies.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">1.338</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mehetre, Gajanan T.</style></author><author><style face="normal" font="default" size="100%">Vinodh, J. S.</style></author><author><style face="normal" font="default" size="100%">Burkul, Bhushan B.</style></author><author><style face="normal" font="default" size="100%">Desai, D.</style></author><author><style face="normal" font="default" size="100%">Santhakumari, B.</style></author><author><style face="normal" font="default" size="100%">Dharne, Mahesh S.</style></author><author><style face="normal" font="default" size="100%">Dastager, Syed G</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Bioactivities and molecular networking-based elucidation of metabolites of potent actinobacterial strains isolated from the Unkeshwar geothermal springs in India</style></title><secondary-title><style face="normal" font="default" size="100%">RSC Advances</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">9850-9859</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;span class=&quot;hitHilite&quot;&gt;The&lt;/span&gt; bioactive potential &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; Actinobacteria endemic &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; hot &lt;span class=&quot;hitHilite&quot;&gt;springs&lt;/span&gt; has rarely been investigated. This study highlights &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; cultivable diversity and &lt;span class=&quot;hitHilite&quot;&gt;bioactivities&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; Actinobacteria associated &lt;span class=&quot;hitHilite&quot;&gt;with&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;Unkeshwar&lt;/span&gt; hot &lt;span class=&quot;hitHilite&quot;&gt;springs&lt;/span&gt;, &lt;span class=&quot;hitHilite&quot;&gt;India&lt;/span&gt;. &lt;span class=&quot;hitHilite&quot;&gt;Potent&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; were evaluated for their biosynthetic potentials and metabolite analysis was performed using effective dereplication &lt;span class=&quot;hitHilite&quot;&gt;molecular&lt;/span&gt; networking tools. &lt;span class=&quot;hitHilite&quot;&gt;A&lt;/span&gt; total &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; 86 &lt;span class=&quot;hitHilite&quot;&gt;actinobacterial&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; were &lt;span class=&quot;hitHilite&quot;&gt;isolated&lt;/span&gt; and grouped &lt;span class=&quot;hitHilite&quot;&gt;into&lt;/span&gt; 21 distinct genera, based &lt;span class=&quot;hitHilite&quot;&gt;on&lt;/span&gt; 16S rRNA gene sequence analysis. These &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; included rare members such as Micromonospora, Marmoricola, Actinomadura, Cellulomonas, Cellulosimicrobium, Janibacter, Rothia, Barrentisimonas, Dietzia and Glycomyces. &lt;span class=&quot;hitHilite&quot;&gt;In&lt;/span&gt; antimicrobial screening, Micromonospora sp. strain GH99 and Streptomyces sp. strain GH176 were found &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; be &lt;span class=&quot;hitHilite&quot;&gt;potent&lt;/span&gt; antimicrobial &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt;. &lt;span class=&quot;hitHilite&quot;&gt;The&lt;/span&gt; metabolic extracts &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; these &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; exhibited strong antimicrobial activity against Staphylococcus epidermidis (NCIM 2493), Shigella flexneri (NCIM 5265), Klebsiella pneumonia (NCIM 2098), and Salmonella abony (NCIM 2257). &lt;span class=&quot;hitHilite&quot;&gt;The&lt;/span&gt; extracts also displayed strong anti-biofilm and anticancer &lt;span class=&quot;hitHilite&quot;&gt;activities&lt;/span&gt; against Pseudomonas aeruginosa (NCIM 5029), Acinetobacter junii (NCIM 5188) and breast cancer cell line MCF7, respectively. Both &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; also tested positive for &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; presence &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; PKS biosynthetic gene cluster &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; their genomes. &lt;span class=&quot;hitHilite&quot;&gt;To&lt;/span&gt; effectively delineate &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; secondary &lt;span class=&quot;hitHilite&quot;&gt;metabolites&lt;/span&gt;, &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; extracts were subjected &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; MS/MS-guided &lt;span class=&quot;hitHilite&quot;&gt;molecular&lt;/span&gt; networking analysis. Structurally diverse compounds including &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; polyketides 22-dehydroxymethyl-kijanolide (GH99 strain) and Abyssomicin I (GH176 strain) were detected &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; extracts. Interestingly, Brevianamide F was detected &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; extract &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; Micromonospora, which has previously been mostly found &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; fungal species. Other compounds such as cyclic tripeptides, Cyclo(l-Pro-d-Ile) and Cyclo(d-Pro-l-Phe), were also identified &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; this strain. &lt;span class=&quot;hitHilite&quot;&gt;In&lt;/span&gt; summary, for &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; first time, we explored &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; diversity &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; Actinobacteria and evaluated their bioactive potential &lt;span class=&quot;hitHilite&quot;&gt;from&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;Unkeshwar&lt;/span&gt; hot &lt;span class=&quot;hitHilite&quot;&gt;springs&lt;/span&gt;. &lt;span class=&quot;hitHilite&quot;&gt;The&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;potent&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;isolated&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; study could be useful &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; drug &lt;span class=&quot;hitHilite&quot;&gt;discovery&lt;/span&gt; programs.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">17</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;&lt;span class=&quot;LrzXr kno-fv&quot;&gt;3.049&lt;/span&gt;&lt;/p&gt;
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mehetre, Gajanan T.</style></author><author><style face="normal" font="default" size="100%">Dastager, Syed G.</style></author><author><style face="normal" font="default" size="100%">Dharne, Mahesh S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Biodegradation of mixed polycyclic aromatic hydrocarbons by pure and mixed cultures of biosurfactant producing thermophilic and thermo-tolerant bacteria</style></title><secondary-title><style face="normal" font="default" size="100%">Science of the Total Environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biodegradation</style></keyword><keyword><style  face="normal" font="default" size="100%">Biosurfactants</style></keyword><keyword><style  face="normal" font="default" size="100%">Crude oil</style></keyword><keyword><style  face="normal" font="default" size="100%">Mixed culture</style></keyword><keyword><style  face="normal" font="default" size="100%">PAHs</style></keyword><keyword><style  face="normal" font="default" size="100%">Thermophiles</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">AUG</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">679</style></volume><pages><style face="normal" font="default" size="100%">52-60</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Applicability of thermophilic and thereto-tolerant microorganisms for biodegradation of polycyclic aromatic hydrocarbons (PAHs) with low water solubility is an interesting strategy for improving the biodegradation efficiency. In this study, we evaluated utility of thermophilic and thermo-tolerant bacteria isolated from Unkeshwar hot spring (India) for biodegradation of four different PAHs. Water samples were enriched in mineral salt medium (MSM) containing a mixture of four PAHs compounds (anthracene: ANT, fluorene: FLU, phenanthrenc: PHE and pyrene: PYR) at 37 degrees C and 50 degrees C. After growth based screening, four potent strains obtained which were identified as Aeribacillus pallidus (UCPS2), Bacillus axarquiensis (UCPD1), Bacillus siamensis (GHP76) and Bacillus subtilis subsp. inaquosorum (U277) based on the 16S rRNA gene sequence analysis. Degradation of mixed PAH compounds was evaluated by pure as well as mixed cultures under shake flask conditions using MSM supplemented with 200 mg/L concentration of PAHs (50 mg/L of each compound) for 15 days at 37 degrees C and 50 degrees C. A relatively higher degradation of ANT (92% - 96%), FLU (83% - 86%), PHE (16% - 54%) and PYR (51% - 71%) was achieved at 50 degrees C by Aeribacillus sp. (UCPS2) and mixed culture. Furthermore, crude oil was used as a substrate to study the degradation of same PAHs using these organisms which also revealed with similar results with the higher degradation at 50 degrees C. Interestingly, PAH-degrading strains were also positive for biosurfactant production. Biosurfactants were identified as the variants of surfactins (lipopeptide biosurfactants) based on analytical tools and phylogenetic analysis of the surfactin genes. Overall, this study has shown that hot spring microbes may have a potential for PAHs degradation and also biosurfactant production at a higher temperature, which could provide a novel perspective for removal of PAHs residues from oil contaminated sites. (C) 2019 Elsevier B.V. All rights reserved.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;5.589&lt;/p&gt;
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Samson, Rachel</style></author><author><style face="normal" font="default" size="100%">Navale, Govinda R.</style></author><author><style face="normal" font="default" size="100%">Dharne, Mahesh S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Biosensors: frontiers in rapid detection of COVID-19</style></title><secondary-title><style face="normal" font="default" size="100%">3 Biotech</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biosensors</style></keyword><keyword><style  face="normal" font="default" size="100%">COVID-19</style></keyword><keyword><style  face="normal" font="default" size="100%">Rapid detection</style></keyword><keyword><style  face="normal" font="default" size="100%">SARS-CoV-2</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">AUG</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">385</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The rapid community-spread of novel human coronavirus 2019 (nCOVID19 or SARS-Cov2) and morbidity statistics has put forth an unprecedented urge for rapid diagnostics for quick and sensitive detection followed by contact tracing and containment strategies, especially when no vaccine or therapeutics are known. Currently, quantitative real-time polymerase chain reaction (qRT-PCR) is being used widely to detect COVID-19 from various types of biological specimens, which is time-consuming, labor-intensive and may not be rapidly deployable in remote or resource-limited settings. This might lead to hindrance in acquiring realistic data of infectivity and community spread of SARS-CoV-2 in the population. This review summarizes the existing status of current diagnostic methods, their possible limitations, and the advantages of biosensor-based diagnostics over the conventional ones for the detection of SARS-Cov-2. Novel biosensors used to detect RNA-viruses include CRISPR-Cas9 based paper strip, nucleic-acid based, aptamer-based, antigen-Au/Ag nanoparticles-based electrochemical biosensor, optical biosensor, and Surface Plasmon Resonance. These could be effective tools for rapid, authentic, portable, and more promising diagnosis in the current pandemic that has affected the world economies and humanity. Present challenges and future perspectives of developing robust biosensors devices for rapid, scalable, and sensitive detection and management of COVID-19 are presented in light of the test-test-test theme of the World Health Organization (WHO).&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">9</style></issue><work-type><style face="normal" font="default" size="100%">Review</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;1.798&lt;/p&gt;
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chavan, Sambhaji B.</style></author><author><style face="normal" font="default" size="100%">Shete, Ashvini M.</style></author><author><style face="normal" font="default" size="100%">Dharne, Mahesh S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Bioprocess optimization of penicillium funiculosum NCIM 1228 for improved production and hydrolytic efficiency of cellulases on sugarcane bagasse</style></title><secondary-title><style face="normal" font="default" size="100%">Sugar Tech</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cellulase</style></keyword><keyword><style  face="normal" font="default" size="100%">Lignocellulosic biomass</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillium funiculosum</style></keyword><keyword><style  face="normal" font="default" size="100%">Response surface methodology</style></keyword><keyword><style  face="normal" font="default" size="100%">Submerged fermentation</style></keyword><keyword><style  face="normal" font="default" size="100%">Xylanase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">FEB</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">26</style></volume><pages><style face="normal" font="default" size="100%">215-233</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The cellulase enzyme is currently the world's third largest commercial enzyme. Because of its requirement in various industries like textiles, food, waste management, pharmaceuticals, agriculture, pulp paper, biofuels, and others, its demand curve has increased sharply. The response surface methodology (RSM) approach was employed to optimize media components and process parameters in the current investigation, which successfully increased the cellulase production from Penicillium funiculosum NCIM 1228. Statistical optimization for the hyperproduction of cellulases was conducted using RSM. The Plackett-Burman design (PBD) approach was used to investigate the critical factors of the cellulase production medium. Subsequently, the Box-Behnken design (BBD) method was used to statistically estimate optimum values and conditions that substantially impacted cellulase production. The estimated optimal combinations of parameters for cellulase production were urea (0.2%), CaCl2 (0.2%), MgSO4 (0.05%), peptone (1.5%), microcrystalline cellulose (5.0%), wheat bran (2.5%), corn steep liquor (CSL) (2.5%), KH2PO4 (0.15%), inoculum (10.65%), agitation (157 rpm), pH (5.88), and temperature (29.84 C-degrees). Conclusively, experimental validation under optimal conditions detected an increased production of 3.82- and 3.61-fold in filter paper assay (FPase) and beta-glucosidase, respectively. Additionally, 1.66- and 1.57-fold enhancement in FPase and beta-glucosidase specific activity was observed where an xylanase activity was enhanced by 3.29-fold. Furthermore, the enzyme showed 51.30 per cent hydrolysis efficiency on sugarcane bagasse lignocellulosic biomass (LCB), at a dose of 7 FPase units per g of cellulose. P. funiculosum NCIM 1228 offers the benefit of producing cellulase with an entire cellulolytic system of enzymes that can be synthesized extracellularly, thus acting as a promising biocatalyst for biofuel industries.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Journal Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;1.9&lt;/p&gt;
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