<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Joshi, Soumai Kant</style></author><author><style face="normal" font="default" size="100%">Bisht, Vandana</style></author><author><style face="normal" font="default" size="100%">Dhar, Uppeandra</style></author><author><style face="normal" font="default" size="100%">Joshi, Meena</style></author><author><style face="normal" font="default" size="100%">Bisht, Anil Kumar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro regeneration of `Green Sweet' apple via nucellus-raised callus</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Horticultural Science &amp; Biotechnology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JUL</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">4</style></number><publisher><style face="normal" font="default" size="100%">HEADLEY BROTHERS LTD</style></publisher><pub-location><style face="normal" font="default" size="100%">INVICTA PRESS, ASHFORD TN24 8HH, KENT, ENGLAND</style></pub-location><volume><style face="normal" font="default" size="100%">83</style></volume><pages><style face="normal" font="default" size="100%">447-452</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;For the first time, an in vitro regeneration system for `Green Sweet' apple has been developed from nucellus-raised callus. Callus induction (100%) occurred in nucellar explants excised from immature fruit when cultivated on Murashige and Skoog (MS) basal medium, free of plant growth regulators (PGR) and supplemented with 3.0% (w/v) sucrose and 0.8% (w/v) agar. When cultured on MS basal medium supplemented with 10.0 mu M benzyladenine (BA) and 1.0 mu M gibberellic acid (GA(3)), calli showed 88.9 +/- 5.6% shoot regeneration with 4.1 +/- 0.1. shoots per explant, and a mean shoot length of 3.5 +/- 0.2 cm. Elongated shoots produced roots (44.4 +/- 5.6%) when placed in half-strength MS basal medium supplemented with 1.0 mu M indole-3-butyric acid (IBA) and 1.0 mu M a-naphthaleneacetic acid (NAA). Maximum rooting percentage (55.6 +/- 5.6%) was obtained on 0.4% (w/v) agar, while the highest number of roots per shoot (2.8 +/- 0.4 roots) and root length (3.4 +/- 0.2 cm) were recorded on 0.6% (w/v) agar. Acclimatisation of in vitro-raised plantlets resulted in 39.1 % surviving plantlets. The findings of this study indicate that nucellus-raised callus of the apple cultivar `Green Sweet' can efficiently regenerate shoots that can be rooted and successfully acclimatised.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;</style></custom3><custom4><style face="normal" font="default" size="100%">0.458</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pandey, Meena</style></author><author><style face="normal" font="default" size="100%">Dhar, Uppeandra</style></author><author><style face="normal" font="default" size="100%">Samant, Sher Singh</style></author><author><style face="normal" font="default" size="100%">Shirgurkar, Mrudul Vijay</style></author><author><style face="normal" font="default" size="100%">Thengane, Shubhada Ratnakar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Recurrent somatic embryogenesis and plant regeneration in Angelica glauca Edgew., a critically endangered medicinal plant of the Western Himalaya</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Horticultural Science &amp; Biotechnology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">SEP</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">5</style></number><publisher><style face="normal" font="default" size="100%">HEADLEY BROTHERS LTD</style></publisher><pub-location><style face="normal" font="default" size="100%">INVICTA PRESS, ASHFORD TN24 8HH, KENT, ENGLAND</style></pub-location><volume><style face="normal" font="default" size="100%">86</style></volume><pages><style face="normal" font="default" size="100%">493-498</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Secondary somatic embryogenesis and plant regeneration from seedling explants of Angelica glauca, an endangered medicinal plant of the Himalaya, is reported for the first time. Callus was obtained from all the explants tested in the present study (i.e., epicotyls, hypocotyls, and cotyledonary nodes). The highest frequency of callus formation (95.8%) was observed using epicotyl explants on 4.0 mu M 2,4-dichlorophenoxyacetic acid (2,4-D), whereas 70.8% of hypocotyl explants, and 58.3% of cotyledonary nodes produced callus. One-hundred percent embryogenic callus was induced from epicotyl explants in 2.0 mu M 6-benzyladenine (BA) and 2.0 mu M alpha-naphthaleneacetic acid (NAA), together with the maximum number of somatic embryos (34.2 embryos per explant). Cotyledonary nodes did not produce somatic embryos. Histological studies confirmed the induction of somatic embryogenesis. Somatic embryos germinated into plantlets upon transfer to half-strength Murashige and Skoog (MS) medium without added plant growth regulators. We observed 85% survival of these plantlets under field conditions. The development of secondary embryos was also observed when primary embryos were sub-cultured on full-strength MS medium containing 2.0 mu M NAA plus 2.0 mu M BA. This system of recurrent somatic embryogenesis provides a route for gene transfer and also for the large-scale production of this critically endangered medicinal plant.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">0.72</style></custom4></record></records></xml>