<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Shriram, Varsha</style></author><author><style face="normal" font="default" size="100%">Kumar, Vinay</style></author><author><style face="normal" font="default" size="100%">Kishor, P. B. Kavi</style></author><author><style face="normal" font="default" size="100%">Suryawanshi, Sharad B.</style></author><author><style face="normal" font="default" size="100%">Upadhyay, Ankur K.</style></author><author><style face="normal" font="default" size="100%">Bhat, Manoj K.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytotoxic activity of 9,10-dihydro-2,5-dimethoxyphenanthrene-1,7-diol from Eulophia nuda against human cancer cells</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Ethnopharmacology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cytotoxic activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Eulophia nuda</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenanthrene derivatives</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">1</style></number><publisher><style face="normal" font="default" size="100%">ELSEVIER IRELAND LTD</style></publisher><pub-location><style face="normal" font="default" size="100%">ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND</style></pub-location><volume><style face="normal" font="default" size="100%">128</style></volume><pages><style face="normal" font="default" size="100%">251-253</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Ethnopharmacological relevance: Eulophia nuda L. (Orchidaceae) is a medicinally important terrestrial orchid used for the treatment of tumours and various health problems by the local healers throughout the Western Ghats region in Maharashtra (India). Aim of the study: To isolate the active molecule from Eulophia nuda and to study its cytotoxic potential against human cancer cells. Materials and methods: The crude methanolic extract of Eulophia nuda tubers was fractionated by stepwise gradient of the solvents chloroform-methanol to isolate the pure compound. Isolated pure compound was assessed for its cytotoxic potential against human breast cancer cell lines, MCF-7 and MDA-MB-231 using MTT assay. Structure elucidation of the isolated active compound was carried out by extensive spectroscopic analysis including (1)H NMR, (13)C NMR, NOESY, COSY, LC-MS and IR. Results: The isolated active molecule was identified as phenanthrene derivative 9,10-dihydro-2,5-dimethoxyphenanthrene-1,7-diol. This compound showed good antiproliferative activity against human breast cancer cell lines MCF-7 (91%) and MDA-MB-231 (85%) at 1000 mu g/ml concentration. Conclusion: 9,10-Dihydro-2,5-dimethoxyphenanthrene-1,7-diol from Eulophia nuda tubers showed good growth suppressive effect against human cancer cell lines MCF-7 and MDA-MB-231 making it a potential biomolecule against human cancer. (C) 2009 Elsevier Ireland Ltd. All rights reserved.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">2.466</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sirisha, V. L.</style></author><author><style face="normal" font="default" size="100%">Prashant, S.</style></author><author><style face="normal" font="default" size="100%">Kumar, D. Ranadheer</style></author><author><style face="normal" font="default" size="100%">Pramod, S.</style></author><author><style face="normal" font="default" size="100%">Jalaja, N.</style></author><author><style face="normal" font="default" size="100%">Kumari, P. Hima</style></author><author><style face="normal" font="default" size="100%">Rao, P. Maheshwari</style></author><author><style face="normal" font="default" size="100%">Rao, S. Nageswara</style></author><author><style face="normal" font="default" size="100%">Mishra, Preeti</style></author><author><style face="normal" font="default" size="100%">Karumanchi, S. Rao</style></author><author><style face="normal" font="default" size="100%">Khan, Bashir Mohammad</style></author><author><style face="normal" font="default" size="100%">Kishor, P. B. Kavi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cloning, characterization and impact of up- and down-regulating subabul cinnamyl alcohol dehydrogenase (CAD) gene on plant growth and lignin profiles in transgenic tobacco</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Growth Regulation</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cinnamyl alcohol dehydrogenase</style></keyword><keyword><style  face="normal" font="default" size="100%">Lignin down-regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Subabul (Leucaena leucocephala)</style></keyword><keyword><style  face="normal" font="default" size="100%">Tobacco</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">APR</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">3</style></number><publisher><style face="normal" font="default" size="100%">SPRINGER</style></publisher><pub-location><style face="normal" font="default" size="100%">VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS</style></pub-location><volume><style face="normal" font="default" size="100%">66</style></volume><pages><style face="normal" font="default" size="100%">239-253</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Both cDNA including 5'UTR and 3'UTR and genomic clones of cinnamyl alcohol dehydrogenase (CAD) were isolated and characterized from a pulp-yielding leguminous tree Leucaena leucocephala (LlCAD1). The deduced amino acid sequence shared high identity with orthologous sequences of Acacia mangium x Acacia auriculiformis (83%), Medicago sativa (83%), Nicotiana tabaccum (83%) and Aralia cordata (81%). Full length cDNA contained 78 bases of 5'UTR and 283 bases of 3'UTR, while the genomic clone contained 5 exons and 4 introns. Western blot analysis revealed elevated expression of LlCAD1 in seedling roots and shoots compared to leaves. Sense and antisense CAD tobacco transgenics showed increased and reduced CAD activity accompanied by a change in monomeric lignin composition. Histochemical staining of lignin in down-regulated plants suggested an increase in aldehyde units and a decrease in S/G ratio. Down-regulation of CAD resulted in accumulation of syringic, ferulic, p-coumaric and sinapic acids compared to untransformed controls. These observations were validated by anatomical studies of down-regulated transgenic stems which showed thin walled, elongated phloem and xylem fibres, accompanied by a reduction in the density of vessel elements and amount of secondary xylem when compared to untransformed plants. Furthermore, Klason lignin analysis of CAD antisense transgenics showed 7-32% reduced lignin and normal phenotype as compared to untransformed plants. Such a reduction was not noticed in up-regulated transgenics. These results demonstrate a unique opportunity to explore the significant role that down-regulation of CAD gene plays in reducing lignin content thereby offering potential benefits to the pulp and paper industry.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">1.99
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