<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Nupura, Hirve</style></author><author><style face="normal" font="default" size="100%">Asmita, Tingare</style></author><author><style face="normal" font="default" size="100%">Sharat, B.</style></author><author><style face="normal" font="default" size="100%">Asmita, Prabhune</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Media optimization for the production of cephalosporin C acylase from a novel bacterial source: alcaligenes xylosoxidans MTCC*491</style></title><secondary-title><style face="normal" font="default" size="100%">Research Journal of Biotechnology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">alcaligenes xylosoxidans MTCC*491</style></keyword><keyword><style  face="normal" font="default" size="100%">cephalosporin C (CPC)</style></keyword><keyword><style  face="normal" font="default" size="100%">cephalosporin C acylase (CCA)</style></keyword><keyword><style  face="normal" font="default" size="100%">fermentation carbon source</style></keyword><keyword><style  face="normal" font="default" size="100%">nitrogen source</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">FEB</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">1</style></number><publisher><style face="normal" font="default" size="100%">RESEARCH JOURNAL BIOTECHNOLOGY</style></publisher><pub-location><style face="normal" font="default" size="100%">SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA</style></pub-location><volume><style face="normal" font="default" size="100%">3</style></volume><pages><style face="normal" font="default" size="100%">16-21</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The Gram-negative bacterium Alcaligenes xylosoxidans MTCC *491 has been studied for the production of cephalosporin C acylase (CCA), a pharmaceutically important enzyme. The enzyme hydrolyses cephalosporin C (CPC), a natural product, to 7-aminocephalosporanic acid, a key intermediate in the production of semisynthetic cephalosporin antibiotics. Effect of medium, pH, temperature, carbon source, nitrogen source and incubation time, on the production of CCA was studied. 7.7 IU/ml activity was obtained when Luria Bertani broth (LB) was used as a fermentation medium. Glucose and galactose increased the enzyme activity by 9 % and 22 %, while all the nitrogen sources tested showed a repressive effect. Maximum CCA activity was observed around the 5(th) day of incubation indicating potential role of CCA in secondary metabolism. pH 8.0 and 40 degrees C were found to be the optimum conditions for acylase activity.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">0.08</style></custom4></record></records></xml>