<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Itkin, M.</style></author><author><style face="normal" font="default" size="100%">Heinig, U.</style></author><author><style face="normal" font="default" size="100%">Tzfadia, O.</style></author><author><style face="normal" font="default" size="100%">Bhide, Amey J.</style></author><author><style face="normal" font="default" size="100%">Shinde, B.</style></author><author><style face="normal" font="default" size="100%">Cardenas, Pablo D.</style></author><author><style face="normal" font="default" size="100%">Bocobza, S. E.</style></author><author><style face="normal" font="default" size="100%">Unger, T.</style></author><author><style face="normal" font="default" size="100%">Malitsky, Sergey</style></author><author><style face="normal" font="default" size="100%">Finkers, R.</style></author><author><style face="normal" font="default" size="100%">Tikunov, Y.</style></author><author><style face="normal" font="default" size="100%">Bovy, A.</style></author><author><style face="normal" font="default" size="100%">Chikate, Y.</style></author><author><style face="normal" font="default" size="100%">Singh, P.</style></author><author><style face="normal" font="default" size="100%">Rogachev, I.</style></author><author><style face="normal" font="default" size="100%">Beekwilder, J.</style></author><author><style face="normal" font="default" size="100%">Giri, Ashok P.</style></author><author><style face="normal" font="default" size="100%">Aharoni, Asaph</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes</style></title><secondary-title><style face="normal" font="default" size="100%">Science</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JUL</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">6142</style></number><publisher><style face="normal" font="default" size="100%">AMER ASSOC ADVANCEMENT SCIENCE</style></publisher><pub-location><style face="normal" font="default" size="100%">1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA</style></pub-location><volume><style face="normal" font="default" size="100%">341</style></volume><pages><style face="normal" font="default" size="100%">175-179</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Steroidal glycoalkaloids (SGAs) such as alpha-solanine found in solanaceous food plants-as, for example, potato-are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6142</style></issue><custom2><style face="normal" font="default" size="100%">&lt;p&gt;Council of Scientific &amp;amp; Industrial Research (CSIR) - India&lt;/p&gt;</style></custom2><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">31.477
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sonawane, P. D.</style></author><author><style face="normal" font="default" size="100%">Pollier, J.</style></author><author><style face="normal" font="default" size="100%">Panda, S.</style></author><author><style face="normal" font="default" size="100%">Szymanski, J.</style></author><author><style face="normal" font="default" size="100%">Massalha, H.</style></author><author><style face="normal" font="default" size="100%">Yona, M.</style></author><author><style face="normal" font="default" size="100%">Unger, T.</style></author><author><style face="normal" font="default" size="100%">Malitsky, S.</style></author><author><style face="normal" font="default" size="100%">Arendt, P.</style></author><author><style face="normal" font="default" size="100%">Pauwels, L.</style></author><author><style face="normal" font="default" size="100%">Almekias-Siegl, E.</style></author><author><style face="normal" font="default" size="100%">Rogachev, I.</style></author><author><style face="normal" font="default" size="100%">Meir, S.</style></author><author><style face="normal" font="default" size="100%">Cardenas, P.D.</style></author><author><style face="normal" font="default" size="100%">Masri, A.</style></author><author><style face="normal" font="default" size="100%">Petrikov, M.</style></author><author><style face="normal" font="default" size="100%">Schaller, H.</style></author><author><style face="normal" font="default" size="100%">Schaffer, A. A.</style></author><author><style face="normal" font="default" size="100%">Kamble, A.</style></author><author><style face="normal" font="default" size="100%">Giri, A. P.</style></author><author><style face="normal" font="default" size="100%">Goossens, A.</style></author><author><style face="normal" font="default" size="100%">Aharoni, A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Plant cholesterol biosynthetic pathway overlaps with phytosterol metabolism</style></title><secondary-title><style face="normal" font="default" size="100%">Nature Plants</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JAN</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">3</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The amount of cholesterol made by many plants is not negligible. Whereas cholesterogenesis in animals was elucidated decades ago, the plant pathway has remained enigmatic. Among other roles, cholesterol is a key precursor for thousands of bioactive plant metabolites, including the well-known Solanum steroidal glycoalkaloids. Integrating tomato transcript and protein co-expression data revealed candidate genes putatively associated with cholesterol biosynthesis. A combination of functional assays including gene silencing, examination of recombinant enzyme activity and yeast mutant complementation suggests the cholesterol pathway comprises 12 enzymes acting in 10 steps. It appears that half of the cholesterogenesis-specific enzymes evolved through gene duplication and divergence from phytosterol biosynthetic enzymes, whereas others act reciprocally in both cholesterol and phytosterol metabolism. Our findings provide a unique example of nature's capacity to exploit existing protein folds and catalytic machineries from primary metabolism to assemble a new, multi-step metabolic pathway. Finally, the engineering of a 'high-cholesterol' model plant underscores the future value of our gene toolbox to produce high-value steroidal compounds via synthetic biology.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%"> 11.471</style></custom4></record></records></xml>