<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gaikaiwari, Raghavendra P.</style></author><author><style face="normal" font="default" size="100%">Wagh, Shilpa A.</style></author><author><style face="normal" font="default" size="100%">Kulkarni, Bhaskar D.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Efficient lipase purification using reverse micellar extraction</style></title><secondary-title><style face="normal" font="default" size="100%">Bioresource Technology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">(Hexadicetyltrimethylammoniumbromide)</style></keyword><keyword><style  face="normal" font="default" size="100%">AOT</style></keyword><keyword><style  face="normal" font="default" size="100%">CTAB</style></keyword><keyword><style  face="normal" font="default" size="100%">Lipase</style></keyword><keyword><style  face="normal" font="default" size="100%">RME</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">ELSEVIER SCI LTD</style></publisher><pub-location><style face="normal" font="default" size="100%">THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND</style></pub-location><volume><style face="normal" font="default" size="100%">108</style></volume><pages><style face="normal" font="default" size="100%">224-230</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Reverse micellar extraction (RME) of enzyme provides an attractive option for conventional method with the potential to achieve purification and concentration in a single step with high yield. This study presents a methodology for optimization of RME with Pseudomonas lipase as model system. Fold-purification, percent recovery and extraction time were the objective functions while the type and concentration of surfactant, contact time, pH, ionic strength, and the ratio of organic to aqueous phase were the decision variables. Under optimized conditions, the AOT (Aerosol OT (bis 2-ethylhexyl) sodium sulfosuccinate)-isooctane system gave a 15-fold purification, 80% recovery and 2.5-fold concentration of the Pseudomonas lipase with process time of 45 min. (C) 2011 Elsevier Ltd. All rights reserved.&lt;/p&gt;</style></abstract><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">4.75
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gaikaiwari, Raghavendra P.</style></author><author><style face="normal" font="default" size="100%">Wagh, Shilpa A.</style></author><author><style face="normal" font="default" size="100%">Kulkarni, Bhaskar D.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Extraction and purification of tannase by reverse micelle system</style></title><secondary-title><style face="normal" font="default" size="100%">Separation and Purification Technology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">CTAB</style></keyword><keyword><style  face="normal" font="default" size="100%">Reverse micellar extraction</style></keyword><keyword><style  face="normal" font="default" size="100%">Tannase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">ELSEVIER SCIENCE BV</style></publisher><pub-location><style face="normal" font="default" size="100%">PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS</style></pub-location><volume><style face="normal" font="default" size="100%">89</style></volume><pages><style face="normal" font="default" size="100%">288-296</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Tannin acyl hydrolase commonly called as tannase (EC 3.1.1.20) is a commercially important enzyme. Partially purified and concentrated tannase is required for commercial applications. Typical objectives of purification process comprise high fold-purification, recovery and concentration. These objectives may be potentially conflicting. Conventional methods of purification require multiple steps which are time consuming and may cause higher loss. Reverse micellar extraction (RME) using ionic surfactants provides an attractive option for concentration and purification of tannase which is a highly hydrophilic glycoprotein. This study presents an optimized methodology for RME and purification of Aspergillus allahabadi intracellular tannase. Fold-purification, percent recovery and extraction time were the objective while the type and concentration of surfactant, contact time, pH, ionic strength, and the ratio of organic to aqueous phase were the decision variables. Some of these parameters were also studied for their effect on back-extraction. Among the surfactants tested, CTAB-isooctane system was found to be suitable. Under optimized conditions, 12.7-fold purification, 81.2% recovery and 3-fold concentration of tannase with a process time of 45 min was obtained. Conventional purification methods provided a higher fold-purification albeit at a much lower enzyme recovery. Further, the conventional method requires a process time of several hours. (c) 2012 Elsevier B.V. All rights reserved.&lt;/p&gt;</style></abstract><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">2.894
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Khokhani, Mrinal</style></author><author><style face="normal" font="default" size="100%">Khomane, Ramdas B.</style></author><author><style face="normal" font="default" size="100%">Kulkarni, Bhaskar D.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Sodium-doped lithium zirconate nano squares: synthesis, characterization and applications for CO2 sequestration</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Sol-Gel Science and Technology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Carbon dioxide</style></keyword><keyword><style  face="normal" font="default" size="100%">CTAB</style></keyword><keyword><style  face="normal" font="default" size="100%">Lithium zirconate</style></keyword><keyword><style  face="normal" font="default" size="100%">Sodium doped lithium zirconate</style></keyword><keyword><style  face="normal" font="default" size="100%">sol-gel</style></keyword><keyword><style  face="normal" font="default" size="100%">TEM</style></keyword><keyword><style  face="normal" font="default" size="100%">XRD</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">FEB</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">2</style></number><publisher><style face="normal" font="default" size="100%">SPRINGER</style></publisher><pub-location><style face="normal" font="default" size="100%">VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS</style></pub-location><volume><style face="normal" font="default" size="100%">61</style></volume><pages><style face="normal" font="default" size="100%">316-320</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Nano squares of sodium-doped lithium zirconate have been synthesized by a simple citrate based sol-gel method at room temperature in the presence of cetyl trimethylammonium bromide (CTAB) as a surfactant. The structural and morphological properties were investigated by X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM) respectively. XRD analysis of sample calcined at 900 A degrees C for 4 h shows the formation of monoclinic lithium zirconate phase. The TEM shows the interesting morphology of formation of nano squares of sodium doped lithium zirconate sample. The carbon dioxide sorption capacity was carried out by TGA analysis at different high temperatures. It is observed that the sample shows similar to 20 wt% carbon dioxide sorption at higher temperature (650 A degrees C) within 20 min which are better than the known reports.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">1.66
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