<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jasti, Lakshmi Swarnalatha</style></author><author><style face="normal" font="default" size="100%">Dola, Sandhya Rani</style></author><author><style face="normal" font="default" size="100%">Fadnavis, Nitin W.</style></author><author><style face="normal" font="default" size="100%">Addepally, Uma</style></author><author><style face="normal" font="default" size="100%">Daniels, Siona</style></author><author><style face="normal" font="default" size="100%">Ponrathnam, Surendra</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Co-immobilized glucose oxidase and beta-galactosidase on bovine serum albumin coated allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer as a biosensor for lactose determination in milk</style></title><secondary-title><style face="normal" font="default" size="100%">Enzyme and Microbial Technology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">beta-Galactosidase</style></keyword><keyword><style  face="normal" font="default" size="100%">Bovine serum albumin</style></keyword><keyword><style  face="normal" font="default" size="100%">Glucose oxidase</style></keyword><keyword><style  face="normal" font="default" size="100%">Immobilization</style></keyword><keyword><style  face="normal" font="default" size="100%">Lactose assay</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">OCT</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">ELSEVIER SCIENCE INC</style></publisher><pub-location><style face="normal" font="default" size="100%">360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA</style></pub-location><volume><style face="normal" font="default" size="100%">64-65</style></volume><pages><style face="normal" font="default" size="100%">67-73</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Bovine serum albumin (BSA) was adsorbed on allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer with 25% crosslink density (AGE-25) at pH 8.0 (16% w/w). The amino, thiol and carboxylic acid functional groups available on protein coated surface were utilized for covalent immobilization of glucose oxidase and beta-galactosidase, both independently, and in a step-wise manner on the same matrix, with no more than 10% loss of enzyme activity during immobilization. Glutaraldehyde cross-linking after immobilization provided stable enzyme preparations. The pH-optima of the immobilized enzymes were similar to those for free enzyme but their thermal stability was vastly improved. The co-immobilized enzyme support was used as a biosensor for determination of lactose in milk with excellent reproducibility and reusability. (C) 2014 Elsevier Inc. All rights reserved.&lt;/p&gt;</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">2.624</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jasti, Lakshmi Swarnalatha</style></author><author><style face="normal" font="default" size="100%">Dola, Sandhya Rani</style></author><author><style face="normal" font="default" size="100%">Kumaraguru, Thenkrishnan</style></author><author><style face="normal" font="default" size="100%">Bajja, Sreedhar</style></author><author><style face="normal" font="default" size="100%">Fadnavis, Nitin W.</style></author><author><style face="normal" font="default" size="100%">Addepally, Uma</style></author><author><style face="normal" font="default" size="100%">Rajdeo, Kishor</style></author><author><style face="normal" font="default" size="100%">Ponrathnam, Surendra</style></author><author><style face="normal" font="default" size="100%">Deokar, Sarika Babasaheb</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Protein-coated polymer as a matrix for enzyme immobilization: immobilization of trypsin on bovine serum albumin-coated allyl glycidyl ether-ethylene glycol dimethacrylate copolymer</style></title><secondary-title><style face="normal" font="default" size="100%">Biotechnology Progress</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">allyl glycidyl ether</style></keyword><keyword><style  face="normal" font="default" size="100%">Bovine serum albumin</style></keyword><keyword><style  face="normal" font="default" size="100%">ethylene glycol dimethacrylate</style></keyword><keyword><style  face="normal" font="default" size="100%">Immobilization</style></keyword><keyword><style  face="normal" font="default" size="100%">Trypsin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">2</style></number><publisher><style face="normal" font="default" size="100%">WILEY-BLACKWELL</style></publisher><pub-location><style face="normal" font="default" size="100%">111 RIVER ST, HOBOKEN 07030-5774, NJ USA</style></pub-location><volume><style face="normal" font="default" size="100%">30</style></volume><pages><style face="normal" font="default" size="100%">317-323</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer with 25% crosslink density (AGE-25) shows excellent bovine serum albumin (BSA) adsorption (up to 16% (w/w)) at pH 8.0 and the adsorbed BSA is strongly bound. This protein-coated polymer provides a novel matrix with naturally existing functional groups such as thiol, amino, and carboxylic acid that are available for covalent immobilization of functional enzymes. Employing appropriate strategies, trypsin as a model protein was covalently bound to BSA-coated matrix both independently, and in a stepwise manner on the same matrix, with less than 5% loss of enzyme activity during immobilization. Glutaraldehyde crosslinking after immobilization provide stable enzyme preparation with activity of 510 units/g recycled up to six times without loss of enzyme activity. AFM studies reveal that the polymer surface has protein peaks and valleys rather than a uniform monolayer distribution of the protein and the immobilized enzyme preparation can best be described as polymer supported cross-linked enzyme aggregates (CLEAs). (c) 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:317-323, 2014&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">2.65</style></custom4></record></records></xml>