<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sharma, Trupti K.</style></author><author><style face="normal" font="default" size="100%">Bhadane, Vaibhav A.</style></author><author><style face="normal" font="default" size="100%">Kumar, Lalitha S.</style></author><author><style face="normal" font="default" size="100%">Rele, Meenakshi V.</style></author><author><style face="normal" font="default" size="100%">Bhawar, Gajanan</style></author><author><style face="normal" font="default" size="100%">Rahman, Imran</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Optimization of the production of a maltooligosaccharides producing amylase from the alkalophilic streptomyces lonarensis strain NCL 716 using SVR modeling</style></title><secondary-title><style face="normal" font="default" size="100%">Starch-Starke</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alkaline a-amylase</style></keyword><keyword><style  face="normal" font="default" size="100%">Optimization</style></keyword><keyword><style  face="normal" font="default" size="100%">Streptomyces</style></keyword><keyword><style  face="normal" font="default" size="100%">Support vector regression analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JAN</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">1-2</style></number><publisher><style face="normal" font="default" size="100%">WILEY-V C H VERLAG GMBH</style></publisher><pub-location><style face="normal" font="default" size="100%">BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY</style></pub-location><volume><style face="normal" font="default" size="100%">65</style></volume><pages><style face="normal" font="default" size="100%">179-185</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The Streptomyces lonarensis strain NCL 716 hydrolyses starch to produce a mixture of maltotriose (G3) and maltotetraose (G4) along with maltose (G2). The objective of the present work was to determine an optimum cost effective media composition for the production of a-amylase from this strain. The most influential factor was found to be starch while the least influential factor found was peptone by PlackettBurman method. Peptone amount was kept constant throughout the fermentation. Peptone, which is one of the expensive media components was used at a concentration of 1?g/L, which made the optimum media composition cost effective. A support vector regression-based process model was developed for approximating the non-linear relationship between the fermentation operating variables and the a-amylase yield. Multicanonical Jump Walk Annealing, a stochastic optimization technique is used to obtain optimal operating variables to maximize amylase yield. The maximum amylase activity thus obtained was in good agreement with the experimental values at the optimized levels. The optimum media composition obtained by this method was: yeast extract: 4.53?g/L, starch: 20.246?g/L, K2HPO4: 0.0827%, MgSO4: 0.15%, peptone: 1?g/L. A maximum enzyme activity of 297?U/mL, which was achieved using the above approaches compares well with the activity of reported amylases producing maltooligosaccharides.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1-2</style></issue><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">1.401
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sharma, Trupti K.</style></author><author><style face="normal" font="default" size="100%">Mawlankar, Rahul R.</style></author><author><style face="normal" font="default" size="100%">Sonalkar, Vidya V.</style></author><author><style face="normal" font="default" size="100%">Shinde, Vidhya K.</style></author><author><style face="normal" font="default" size="100%">Zhan, Jing</style></author><author><style face="normal" font="default" size="100%">Li, Wen-Jun</style></author><author><style face="normal" font="default" size="100%">Rele, Meenakshi V.</style></author><author><style face="normal" font="default" size="100%">Dastager, Syed Gulam</style></author><author><style face="normal" font="default" size="100%">Kumar, Lalitha Sunil</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Streptomyces lonarensis sp nov., isolated from lonar lake, a meteorite salt water lake in India</style></title><secondary-title><style face="normal" font="default" size="100%">Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alkaliphile</style></keyword><keyword><style  face="normal" font="default" size="100%">DDH analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Polyphasic taxonomy</style></keyword><keyword><style  face="normal" font="default" size="100%">Streptomyces lonarensis sp nov.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">FEB</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">2</style></number><publisher><style face="normal" font="default" size="100%">SPRINGER</style></publisher><pub-location><style face="normal" font="default" size="100%">VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS</style></pub-location><volume><style face="normal" font="default" size="100%">109</style></volume><pages><style face="normal" font="default" size="100%">225-235</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A novel alkaliphilic actinomycete, strain NCL716(T), was isolated from a soil sample collected from the vicinity of Lonar Lake, an alkaline salt water meteorite lake in Buldhana district of Maharashtra State in India. The strain was characterised using a polyphasic taxonomic approach which confirmed that it belongs to the genus Streptomyces. Growth was observed over a pH range of 7-11 at 28 A degrees C. The cell wall was found to contain ll-diaminopimelic acid and traces of meso-diaminopimelic acid. The major fatty acid components were identified as iso-C-16:0 (46.8 %), C-17:1 (12.4 %), anteiso-C-15:0 (5.1 %) and anteiso-C-17:1 (4.8 %). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The major menaquinones were determined to be MK-9 (H-6) (70.3 %), MK-9 (H-4) (15.5 %) and MK-9 (H-8) (7.2 %). The G+C content of the DNA of the type strain was determined to be 71.4 mol %. The 16S rRNA gene sequence has been deposited in GenBank with accession number FJ919811. Although the 16S rRNA gene sequence analysis revealed that strain NCL716(T) shares &amp;gt; 99 % similarity with that of Streptomyces bohaiensis strain 11A07(T), DNA-DNA hybridization revealed only 33.2 +/- A 3.0 % relatedness between them. Moreover, these two strains can be readily distinguished by some distinct phenotypic characteristics. Hence, on the basis of phenotypic and genetic analyses, it is proposed that strain NCL716(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces lonarensis sp. nov., is proposed. The type strain is NCL 716(T) (=DSM 42084(T) = MTCC 11708(T) = KCTC 39684(T)).&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;</style></custom3><custom4><style face="normal" font="default" size="100%">1.944</style></custom4></record></records></xml>