<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Shinde, Balkrishna A.</style></author><author><style face="normal" font="default" size="100%">Dholakia, Bhushan B.</style></author><author><style face="normal" font="default" size="100%">Hussain, Khalid</style></author><author><style face="normal" font="default" size="100%">Aharoni, Asaph</style></author><author><style face="normal" font="default" size="100%">Giri, Ashok P.</style></author><author><style face="normal" font="default" size="100%">Kamble, Avinash C.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Biotechnology Journal </style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alternaria solani</style></keyword><keyword><style  face="normal" font="default" size="100%">early blight</style></keyword><keyword><style  face="normal" font="default" size="100%">Solanum arcanum</style></keyword><keyword><style  face="normal" font="default" size="100%">Tomato</style></keyword><keyword><style  face="normal" font="default" size="100%">WRKY1</style></keyword><keyword><style  face="normal" font="default" size="100%">XTH5</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">AUG</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">1502-1513</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Early blight (EB), caused by Alternaria solani, is a major threat to global tomato production. In comparison with cultivated tomato (Solanum lycopersicum), a wild relative, S.arcanum exhibits strong resistance against EB. However, molecular cascades operating during EB resistance in wild or cultivated tomato plants are largely obscure. Here, we provide novel insight into spatio-temporal molecular events in S.arcanum against A.solani. Transcriptome and co-expression analysis presented 33-WRKYs as promising candidates of which 12 SaWRKYs displayed differential expression patterns in resistant and susceptible accessions during EB disease progression. Among these, SaWRKY1 exhibited induced expression with significant modulation in xyloglucan endotrans hydrolase 5 (XTH5) and MYB2 expressions that correlated with the disease phenotypes. Electro-mobility shift assay confirmed physical interaction of recombinant SaWRKY1 to SaXTH5 and SaMYB2 promoters. Comparative WRKY1 promoter analysis between resistant and susceptible plants revealed the presence of crucial motifs for defence mechanism exclusively in resistant accession. Additionally, many defence-related genes displayed significant expression variations in both the accessions. Further, WRKY1 overexpressing transgenic plants exhibited higher levels of EB resistance while RNAi silencing lines had increased susceptibility to A.solani with altered expression of XTH5 and MYB2. Overall, these findings demonstrate the positive influence of WRKY1 in improving EB resistance in wild tomato and this could be further utilized as a potential target through genetic engineering to augment protection against A.solani in crop plants.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">7.443</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sonawane, Prashant D.</style></author><author><style face="normal" font="default" size="100%">Jozawiak, Adam</style></author><author><style face="normal" font="default" size="100%">Barbole, Ranjit</style></author><author><style face="normal" font="default" size="100%">Panda, Sayantan</style></author><author><style face="normal" font="default" size="100%">Abebie, Bekele</style></author><author><style face="normal" font="default" size="100%">Kazachkova, Yana</style></author><author><style face="normal" font="default" size="100%">Gharat, Sachin A.</style></author><author><style face="normal" font="default" size="100%">Ramot, Ofir</style></author><author><style face="normal" font="default" size="100%">Unger, Tamar</style></author><author><style face="normal" font="default" size="100%">Wizler, Guy</style></author><author><style face="normal" font="default" size="100%">Meir, Sagit</style></author><author><style face="normal" font="default" size="100%">Rogachev, Ilana</style></author><author><style face="normal" font="default" size="100%">Doron-Faigenboim, Adi</style></author><author><style face="normal" font="default" size="100%">Petreikov, Marina</style></author><author><style face="normal" font="default" size="100%">Schaffer, Arthur</style></author><author><style face="normal" font="default" size="100%">Giri, Ashok P.</style></author><author><style face="normal" font="default" size="100%">Scherf, Tali</style></author><author><style face="normal" font="default" size="100%">Aharoni, Asaph</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">2-oxoglutarate-dependent dioxygenases drive expansion of steroidal alkaloid structural diversity in the genus Solanum</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Solanum</style></keyword><keyword><style  face="normal" font="default" size="100%">specialized metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">steroidal glycoalkaloids (SGAs)</style></keyword><keyword><style  face="normal" font="default" size="100%">structural diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">Tomato</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAY</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">234</style></volume><pages><style face="normal" font="default" size="100%">1394-1410</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;
	Solanum steroidal glycoalkaloids (SGAs) are renowned defence metabolites exhibiting spectacular structural diversity. Genes and enzymes generating the SGA precursor pathway, SGA scaffold and glycosylated forms have been largely identified. Yet, the majority of downstream metabolic steps creating the vast repertoire of SGAs remain untapped. Here, we discovered that members of the 2-OXOGLUTARATE-DEPENDENT DIOXYGENASE (2-ODD) family play a prominent role in SGA metabolism, carrying out three distinct backbone-modifying oxidative steps in addition to the three formerly reported pathway reactions. The GLYCOALKALOID METABOLISM34 (GAME34) enzyme catalyses the conversion of core SGAs to habrochaitosides in wild tomato S. habrochaites. Cultivated tomato plants overexpressing GAME34 ectopically accumulate habrochaitosides. These habrochaitoside enriched plants extracts potently inhibit Puccinia spp. spore germination, a significant Solanaceae crops fungal pathogen. Another 2-ODD enzyme, GAME33, acts as a desaturase (via hydroxylation and E/F ring rearrangement) forming unique, yet unreported SGAs. Conversion of bitter alpha-tomatine to ripe fruit, nonbitter SGAs (e.g. esculeoside A) requires two hydroxylations; while the known GAME31 2-ODD enzyme catalyses hydroxytomatine formation, we find that GAME40 catalyses the penultimate step in the pathway and generates acetoxy-hydroxytomatine towards esculeosides accumulation. Our results highlight the significant contribution of 2-ODD enzymes to the remarkable structural diversity found in plant steroidal specialized metabolism.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;
	Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;
	10.323&lt;/p&gt;
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Panda, Sayantan</style></author><author><style face="normal" font="default" size="100%">Jozwiak, Adam</style></author><author><style face="normal" font="default" size="100%">Sonawane, Prashant D.</style></author><author><style face="normal" font="default" size="100%">Szymanski, Jedrzej</style></author><author><style face="normal" font="default" size="100%">Kazachkova, Yana</style></author><author><style face="normal" font="default" size="100%">Vainer, Andrii</style></author><author><style face="normal" font="default" size="100%">Kilambi, Himabindu Vasuki</style></author><author><style face="normal" font="default" size="100%">Almekias-Siegl, Efrat</style></author><author><style face="normal" font="default" size="100%">Dikaya, Varvara</style></author><author><style face="normal" font="default" size="100%">Bocobza, Samuel</style></author><author><style face="normal" font="default" size="100%">Shohat, Hagai</style></author><author><style face="normal" font="default" size="100%">Meir, Sagit</style></author><author><style face="normal" font="default" size="100%">Wizler, Guy</style></author><author><style face="normal" font="default" size="100%">Giri, Ashok P.</style></author><author><style face="normal" font="default" size="100%">Schuurink, Robert</style></author><author><style face="normal" font="default" size="100%">Weiss, David</style></author><author><style face="normal" font="default" size="100%">Yasuor, Hagai</style></author><author><style face="normal" font="default" size="100%">Kamble, Avinash</style></author><author><style face="normal" font="default" size="100%">Aharoni, Asaph</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Steroidal alkaloids defence metabolism and plant growth are modulated by the joint action of gibberellin and jasmonate signalling</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">alpha-tomatine</style></keyword><keyword><style  face="normal" font="default" size="100%">defence</style></keyword><keyword><style  face="normal" font="default" size="100%">gibberellin (GA)</style></keyword><keyword><style  face="normal" font="default" size="100%">growth</style></keyword><keyword><style  face="normal" font="default" size="100%">jasmonate (JA)</style></keyword><keyword><style  face="normal" font="default" size="100%">steroidal glycoalkaloids (SGAs)</style></keyword><keyword><style  face="normal" font="default" size="100%">Tomato</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">FEB</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">233</style></volume><pages><style face="normal" font="default" size="100%">1220-1237</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Steroidal glycoalkaloids (SGAs) are protective metabolites constitutively produced by Solanaceae species. Genes and enzymes generating the vast structural diversity of SGAs have been largely identified. Yet, mechanisms of hormone pathways coordinating defence (jasmonate; JA) and growth (gibberellin; GA) controlling SGAs metabolism remain unclear. We used tomato to decipher the hormonal regulation of SGAs metabolism during growth vs defence tradeoff. This was performed by genetic and biochemical characterisation of different JA and GA pathways components, coupled with in vitro experiments to elucidate the crosstalk between these hormone pathways mediating SGAs metabolism. We discovered that reduced active JA results in decreased SGA production, while low levels of GA or its receptor led to elevated SGA accumulation. We showed that MYC1 and MYC2 transcription factors mediate the JA/GA crosstalk by transcriptional activation of SGA biosynthesis and GA catabolism genes. Furthermore, MYC1 and MYC2 transcriptionally regulate the GA signalling suppressor DELLA that by itself interferes in JA-mediated SGA control by modulating MYC activity through protein-protein interaction. Chemical and fungal pathogen treatments reinforced the concept of JA/GA crosstalk during SGA metabolism. These findings revealed the mechanism of JA/GA interplay in SGA biosynthesis to balance the cost of chemical defence with growth.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">10.151</style></custom4></record></records></xml>