<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sen, Pooja</style></author><author><style face="normal" font="default" size="100%">Gupta, Lovely</style></author><author><style face="normal" font="default" size="100%">Chauhan, Aastha</style></author><author><style face="normal" font="default" size="100%">Goswami, Lakshmi</style></author><author><style face="normal" font="default" size="100%">Bhattacharya, Asish K.</style></author><author><style face="normal" font="default" size="100%">Sengupta, Abhishek</style></author><author><style face="normal" font="default" size="100%">Vijayaraghavan, Pooja</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comprehensive in vitro and in silico assessment of eugenol glycoconjugates against azole and amphotericin B resistant Rhizopus spp.</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular Biology Reports</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antifungals</style></keyword><keyword><style  face="normal" font="default" size="100%">Eugenol</style></keyword><keyword><style  face="normal" font="default" size="100%">Glycoconjugates</style></keyword><keyword><style  face="normal" font="default" size="100%">Mucormycosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Virulence</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JUN </style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">52</style></volume><pages><style face="normal" font="default" size="100%">589</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;
	Background Rhizopus spp. is a major cause of mucormycosis, a severe infectious disease with high morbidity and mortality. Treatment is challenging due to rising antifungal resistance. Glycosylation is a crucial technique for enhancing the properties of phenolic compounds like eugenol. The present study aims to examine the antifungal efficacy of eugenol glycoconjugates against azole and amphotericin B-resistant Rhizopus isolates. Methods and results Out of 50 soil samples, 12 isolates belonging to Mucorales were obtained, of which 7 were identified as Rhizopus spp. via 18S ITS sequencing. Antifungal susceptibility testing (AST) revealed that all Rhizopus isolates were resistant to amphotericin B (MIC &amp;gt; 1 mu g/mL). Most isolates also showed resistance towards posaconazole (MIC &amp;gt; 1 mu g/mL) and itraconazole (MIC &amp;gt; 2 mu g/mL). AST of eugenol glycoconjugate (coded 6g) showed efficacy against resistant Rhizopus isolates, with MIC values ranging from 6.25 mu g/mL to 25 mu g/mL. Flow cytometry confirmed its fungicidal activity, correlating with MIC data. Compound 6g significantly reduced conidial germination within 24 h and exhibited no cytotoxicity on A549 lung cancer cells. In-silico analysis revealed a negative binding affinity of compound 6g for the spore coat protein CotH3, which could be a potential antifungal target. Conclusion Compound 6g could be an potential antifungal molecule against resistant Rhizopus spp, which requires further studies.&lt;/p&gt;
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