<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Khobragade, Taresh P.</style></author><author><style face="normal" font="default" size="100%">Giri, Pritam</style></author><author><style face="normal" font="default" size="100%">Patil, Mahesh D.</style></author><author><style face="normal" font="default" size="100%">Joo, Sangwoo</style></author><author><style face="normal" font="default" size="100%">Cho, Sunga</style></author><author><style face="normal" font="default" size="100%">Kim, Yechan</style></author><author><style face="normal" font="default" size="100%">Ghosh, Rohan</style></author><author><style face="normal" font="default" size="100%">Jeong, Sanghun</style></author><author><style face="normal" font="default" size="100%">Maeng, Minyeong</style></author><author><style face="normal" font="default" size="100%">Song, Min-Ho</style></author><author><style face="normal" font="default" size="100%">Park, Jeong-Min</style></author><author><style face="normal" font="default" size="100%">Lee, Eun Ho</style></author><author><style face="normal" font="default" size="100%">Keum, Young-Soo</style></author><author><style face="normal" font="default" size="100%">Kang, Taek Jin</style></author><author><style face="normal" font="default" size="100%">Heo, Yong-Seok</style></author><author><style face="normal" font="default" size="100%">Yun, Hyungdon</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Total biocatalytic synthesis of capsaicinoids using ferulic acid: a versatile two-step strategy for natural product diversification</style></title><secondary-title><style face="normal" font="default" size="100%">Angewandte Chemie-International Edition</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Capsaicinoids</style></keyword><keyword><style  face="normal" font="default" size="100%">Carboxylic acid reductase</style></keyword><keyword><style  face="normal" font="default" size="100%">Transaminase</style></keyword><keyword><style  face="normal" font="default" size="100%">Vanillylamine</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">DEC </style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">64</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;
	The wide-ranging application of capsaicinoids, the active compounds in chili peppers, has driven increasing interest in the development of sustainable production strategies. However, capsaicinoid synthesis remains a challenge. The objective of this pioneering study is to report the total biocatalytic synthesis of structurally diverse capsaicinoids from bio-based ferulic acids. An X-ray crystallographic study elucidated the structural basis for the exceptional potential of a novel transaminase from Phaeobacter porticola (PPTA) to transform the highest ever reported concentration of vanillin (100-200 mM) to vanillylamine, with &amp;gt;99% conversion and modest conversion ranging from 48% to 79% for 300 to 500 mM substrate. Using PPTA in tandem with phenolic acid decarboxylase (PAD) and aromatic dioxygenase (ADO) further enabled the direct synthesis of vanillylamine from ferulic acid with &amp;gt;99% conversion. Furthermore, the integration of a multi-enzymatic cascade with carboxylic acid reductases (CARs) successfully synthesized structurally diverse capsaicinoids via amide bond formation between vanillylamine and free fatty acids, with excellent conversions ranging from 72% to &amp;gt;88%. A 50-mM enzymatic reaction afforded 95% and 80% conversion of vanillylamine and capsaicin, respectively.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">49</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
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