<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Parekh, Nimisha</style></author><author><style face="normal" font="default" size="100%">Bijosh, C. K.</style></author><author><style face="normal" font="default" size="100%">Kane, Kartiki</style></author><author><style face="normal" font="default" size="100%">Panicker, Alaka</style></author><author><style face="normal" font="default" size="100%">Nisal, Anuya</style></author><author><style face="normal" font="default" size="100%">Wangikar, Pralhad</style></author><author><style face="normal" font="default" size="100%">Agawane, Sachin</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Superior processability of Antheraea mylitta silk with cryo-milling: performance in bone tissue regeneration</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Biological Macromolecules</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bone tissue engineering</style></keyword><keyword><style  face="normal" font="default" size="100%">Calvarial defect</style></keyword><keyword><style  face="normal" font="default" size="100%">hMSCs</style></keyword><keyword><style  face="normal" font="default" size="100%">Non-mulberry silk fiber</style></keyword><keyword><style  face="normal" font="default" size="100%">Processing technique</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JUL</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">213</style></volume><pages><style face="normal" font="default" size="100%">155-165</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;
	Non-mulberry silk polymers have a promising future in biomedical applications. However, the dissolution of nonmulberry silk fiber is a still challenge and this poor processability has limited the use of this material. Here, we report a unique protocol to process the Antheraea mylitta (AM) silk fiber. We have shown that the cryo-milling of silk fiber reduces the beta sheet content by more than 10% and results in an SF powder that completely dissolves in routine solvents like trifluoroacetic acid (TFA) within few hours to form highly concentrated solutions (\~20 wt %). Further, these solutions can be processed using conventional processing techniques such as electrospinning to form 3D scaffolds. Bombyx mori (BM) silk was used as a control sample in the study. In-vitro studies were also performed to monitor cell adhesion and proliferation and hMSCs differentiation into osteogenic lineage. Finally, the osteogenic potential of the scaffolds was also evaluated by a 4-week implantation study in rat calvarial model. The in-vitro and in-vivo results show that the processing techniques do not affect the biocompatibility of the material and the AM scaffolds support bone regeneration. Our results, thus, show that cryo-milling facilitates enhanced processability of non-mulberry silk and therefore expands its potential in biomedical applications.&lt;/p&gt;
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	Foreign&lt;/p&gt;
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	8.025&lt;/p&gt;
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