<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Panda, Sayantan</style></author><author><style face="normal" font="default" size="100%">Jozwiak, Adam</style></author><author><style face="normal" font="default" size="100%">Sonawane, Prashant D.</style></author><author><style face="normal" font="default" size="100%">Szymanski, Jedrzej</style></author><author><style face="normal" font="default" size="100%">Kazachkova, Yana</style></author><author><style face="normal" font="default" size="100%">Vainer, Andrii</style></author><author><style face="normal" font="default" size="100%">Kilambi, Himabindu Vasuki</style></author><author><style face="normal" font="default" size="100%">Almekias-Siegl, Efrat</style></author><author><style face="normal" font="default" size="100%">Dikaya, Varvara</style></author><author><style face="normal" font="default" size="100%">Bocobza, Samuel</style></author><author><style face="normal" font="default" size="100%">Shohat, Hagai</style></author><author><style face="normal" font="default" size="100%">Meir, Sagit</style></author><author><style face="normal" font="default" size="100%">Wizler, Guy</style></author><author><style face="normal" font="default" size="100%">Giri, Ashok P.</style></author><author><style face="normal" font="default" size="100%">Schuurink, Robert</style></author><author><style face="normal" font="default" size="100%">Weiss, David</style></author><author><style face="normal" font="default" size="100%">Yasuor, Hagai</style></author><author><style face="normal" font="default" size="100%">Kamble, Avinash</style></author><author><style face="normal" font="default" size="100%">Aharoni, Asaph</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Steroidal alkaloids defence metabolism and plant growth are modulated by the joint action of gibberellin and jasmonate signalling</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">alpha-tomatine</style></keyword><keyword><style  face="normal" font="default" size="100%">defence</style></keyword><keyword><style  face="normal" font="default" size="100%">gibberellin (GA)</style></keyword><keyword><style  face="normal" font="default" size="100%">growth</style></keyword><keyword><style  face="normal" font="default" size="100%">jasmonate (JA)</style></keyword><keyword><style  face="normal" font="default" size="100%">steroidal glycoalkaloids (SGAs)</style></keyword><keyword><style  face="normal" font="default" size="100%">Tomato</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">FEB</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">233</style></volume><pages><style face="normal" font="default" size="100%">1220-1237</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Steroidal glycoalkaloids (SGAs) are protective metabolites constitutively produced by Solanaceae species. Genes and enzymes generating the vast structural diversity of SGAs have been largely identified. Yet, mechanisms of hormone pathways coordinating defence (jasmonate; JA) and growth (gibberellin; GA) controlling SGAs metabolism remain unclear. We used tomato to decipher the hormonal regulation of SGAs metabolism during growth vs defence tradeoff. This was performed by genetic and biochemical characterisation of different JA and GA pathways components, coupled with in vitro experiments to elucidate the crosstalk between these hormone pathways mediating SGAs metabolism. We discovered that reduced active JA results in decreased SGA production, while low levels of GA or its receptor led to elevated SGA accumulation. We showed that MYC1 and MYC2 transcription factors mediate the JA/GA crosstalk by transcriptional activation of SGA biosynthesis and GA catabolism genes. Furthermore, MYC1 and MYC2 transcriptionally regulate the GA signalling suppressor DELLA that by itself interferes in JA-mediated SGA control by modulating MYC activity through protein-protein interaction. Chemical and fungal pathogen treatments reinforced the concept of JA/GA crosstalk during SGA metabolism. These findings revealed the mechanism of JA/GA interplay in SGA biosynthesis to balance the cost of chemical defence with growth.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">10.151</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sonawane, Prashant D.</style></author><author><style face="normal" font="default" size="100%">Gharat, Sachin A.</style></author><author><style face="normal" font="default" size="100%">Jozwiak, Adam</style></author><author><style face="normal" font="default" size="100%">Barbole, Ranjit</style></author><author><style face="normal" font="default" size="100%">Heinicke, Sarah</style></author><author><style face="normal" font="default" size="100%">Almekias-Siegl, Efrat</style></author><author><style face="normal" font="default" size="100%">Meir, Sagit</style></author><author><style face="normal" font="default" size="100%">Rogachev, Ilana</style></author><author><style face="normal" font="default" size="100%">O'Connor, Sarah E.</style></author><author><style face="normal" font="default" size="100%">Giri, Ashok P.</style></author><author><style face="normal" font="default" size="100%">Aharoni, Asaph</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">BAHD-type acyltransferase concludes the biosynthetic pathway of non-bitter glycoalkaloids in ripe tomato fruit</style></title><secondary-title><style face="normal" font="default" size="100%">Nature Communications</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JUL</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">4540</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;
	During tomato fruit ripening, bitter and toxic steroidal glycoalkaloids (SGAs) are converted to nonbitter and less toxic forms, but proposed acylating enzyme in pathway remain unknown. Here, authors report BAHD-type acyltransferase that catalyze acylation step in biosynthesis of non-bitter SGAs in tomato. Tomato is the highest value fruit and vegetable crop worldwide, yet produces &amp;amp; alpha;-tomatine, a renowned toxic and bitter-tasting anti-nutritional steroidal glycoalkaloid (SGA) involved in plant defense. A suite of modifications during tomato fruit maturation and ripening converts &amp;amp; alpha;-tomatine to the non-bitter and less toxic Esculeoside A. This important metabolic shift prevents bitterness and toxicity in ripe tomato fruit. While the enzymes catalyzing glycosylation and hydroxylation reactions in the Esculeoside A pathway have been resolved, the proposed acetylating step remains, to date, elusive. Here, we discovered that GAME36 (GLYCOALKALOID METABOLISM36), a BAHD-type acyltransferase catalyzes SGA-acetylation in cultivated and wild tomatoes. This finding completes the elucidation of the core Esculeoside A biosynthetic pathway in ripe tomato, allowing reconstitution of Esculeoside A production in heterologous microbial and plant hosts. The involvement of GAME36 in bitter SGA detoxification pathway points to a key role in the evolution of sweet-tasting tomato as well as in the domestication and breeding of modern cultivated tomato fruit.&lt;/p&gt;
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	Foreign&lt;/p&gt;
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	16.6&lt;/p&gt;
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