<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sawant, Amol Muralidhar</style></author><author><style face="normal" font="default" size="100%">Vamkudoth, Koteswara Rao</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comprehensive investigation on statistical approaches and classical strain improvement for penicillin V production by Penicillium rubens BIONCL P45 strain</style></title><secondary-title><style face="normal" font="default" size="100%">Systems Microbiology and Biomanufacturing </style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Box-Behnken design</style></keyword><keyword><style  face="normal" font="default" size="100%">Classical strain improvement (CSI)</style></keyword><keyword><style  face="normal" font="default" size="100%">Media optimization</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillin V</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillium rubens</style></keyword><keyword><style  face="normal" font="default" size="100%">Response surface methodology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JAN</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">5</style></volume><pages><style face="normal" font="default" size="100%">1302 -1327</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;
	&lt;span style=&quot;color: rgb(51, 51, 51); font-family: Calibri, airal; font-size: 14px; text-align: justify; background-color: rgb(251, 251, 251);&quot;&gt;Media engineering and strain improvement are critical aspects of microbial biotechnology playing a vital role in enhancing microbial productivity, and ensuring cost-effective bioprocessing. In this investigation, we optimized the various medium components, nutritional condition, and fermentation parameters for the industrial production of phenoxymethylpenicillin or penicillin V (PenV). We have isolated, characterized&amp;nbsp;&lt;/span&gt;&lt;i style=&quot;box-sizing: border-box; margin: 0px; padding: 0px; font-family: Calibri, airal; color: rgb(51, 51, 51); font-size: 14px; text-align: justify; background-color: rgb(251, 251, 251);&quot;&gt;Penicillium rubens&lt;/i&gt;&lt;span style=&quot;color: rgb(51, 51, 51); font-family: Calibri, airal; font-size: 14px; text-align: justify; background-color: rgb(251, 251, 251);&quot;&gt;&amp;nbsp;BIONCL P45 strain which initially produced 100&amp;nbsp;mg/L of PenV. Further, optimization using production medium 4 (PM4) comprising lactose, corn steep solids, sodium sulfate, calcium carbonate, and phenoxy acetic acid lead to a significant increase in production, reaching 430&amp;nbsp;mg/L. Further improvements through response surface methodology (RSM) predicted a production of 646&amp;nbsp;mg/L, which was experimentally validated at 685&amp;nbsp;mg/L. Subsequently, mutagenesis studies using UV (ultraviolet) exposure resulted in the UV-65 mutant, which demonstrated a superior performance, achieving 934&amp;nbsp;mg/L, surpassing the parental strain. ​These combined strategies lead to a tenfold increase in PenV titer, highlighting their effectiveness in bioprocess development and industrial-scale antibiotic production.&lt;/span&gt;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;
	Foreign&lt;/p&gt;
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	3.5&lt;/p&gt;
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