<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mehetre, Gajanan T.</style></author><author><style face="normal" font="default" size="100%">Vinodh, J. S.</style></author><author><style face="normal" font="default" size="100%">Burkul, Bhushan B.</style></author><author><style face="normal" font="default" size="100%">Desai, D.</style></author><author><style face="normal" font="default" size="100%">Santhakumari, B.</style></author><author><style face="normal" font="default" size="100%">Dharne, Mahesh S.</style></author><author><style face="normal" font="default" size="100%">Dastager, Syed G</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Bioactivities and molecular networking-based elucidation of metabolites of potent actinobacterial strains isolated from the Unkeshwar geothermal springs in India</style></title><secondary-title><style face="normal" font="default" size="100%">RSC Advances</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">9850-9859</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;span class=&quot;hitHilite&quot;&gt;The&lt;/span&gt; bioactive potential &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; Actinobacteria endemic &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; hot &lt;span class=&quot;hitHilite&quot;&gt;springs&lt;/span&gt; has rarely been investigated. This study highlights &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; cultivable diversity and &lt;span class=&quot;hitHilite&quot;&gt;bioactivities&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; Actinobacteria associated &lt;span class=&quot;hitHilite&quot;&gt;with&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;Unkeshwar&lt;/span&gt; hot &lt;span class=&quot;hitHilite&quot;&gt;springs&lt;/span&gt;, &lt;span class=&quot;hitHilite&quot;&gt;India&lt;/span&gt;. &lt;span class=&quot;hitHilite&quot;&gt;Potent&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; were evaluated for their biosynthetic potentials and metabolite analysis was performed using effective dereplication &lt;span class=&quot;hitHilite&quot;&gt;molecular&lt;/span&gt; networking tools. &lt;span class=&quot;hitHilite&quot;&gt;A&lt;/span&gt; total &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; 86 &lt;span class=&quot;hitHilite&quot;&gt;actinobacterial&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; were &lt;span class=&quot;hitHilite&quot;&gt;isolated&lt;/span&gt; and grouped &lt;span class=&quot;hitHilite&quot;&gt;into&lt;/span&gt; 21 distinct genera, based &lt;span class=&quot;hitHilite&quot;&gt;on&lt;/span&gt; 16S rRNA gene sequence analysis. These &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; included rare members such as Micromonospora, Marmoricola, Actinomadura, Cellulomonas, Cellulosimicrobium, Janibacter, Rothia, Barrentisimonas, Dietzia and Glycomyces. &lt;span class=&quot;hitHilite&quot;&gt;In&lt;/span&gt; antimicrobial screening, Micromonospora sp. strain GH99 and Streptomyces sp. strain GH176 were found &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; be &lt;span class=&quot;hitHilite&quot;&gt;potent&lt;/span&gt; antimicrobial &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt;. &lt;span class=&quot;hitHilite&quot;&gt;The&lt;/span&gt; metabolic extracts &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; these &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; exhibited strong antimicrobial activity against Staphylococcus epidermidis (NCIM 2493), Shigella flexneri (NCIM 5265), Klebsiella pneumonia (NCIM 2098), and Salmonella abony (NCIM 2257). &lt;span class=&quot;hitHilite&quot;&gt;The&lt;/span&gt; extracts also displayed strong anti-biofilm and anticancer &lt;span class=&quot;hitHilite&quot;&gt;activities&lt;/span&gt; against Pseudomonas aeruginosa (NCIM 5029), Acinetobacter junii (NCIM 5188) and breast cancer cell line MCF7, respectively. Both &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; also tested positive for &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; presence &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; PKS biosynthetic gene cluster &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; their genomes. &lt;span class=&quot;hitHilite&quot;&gt;To&lt;/span&gt; effectively delineate &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; secondary &lt;span class=&quot;hitHilite&quot;&gt;metabolites&lt;/span&gt;, &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; extracts were subjected &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; MS/MS-guided &lt;span class=&quot;hitHilite&quot;&gt;molecular&lt;/span&gt; networking analysis. Structurally diverse compounds including &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; polyketides 22-dehydroxymethyl-kijanolide (GH99 strain) and Abyssomicin I (GH176 strain) were detected &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; extracts. Interestingly, Brevianamide F was detected &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; extract &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; Micromonospora, which has previously been mostly found &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; fungal species. Other compounds such as cyclic tripeptides, Cyclo(l-Pro-d-Ile) and Cyclo(d-Pro-l-Phe), were also identified &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; this strain. &lt;span class=&quot;hitHilite&quot;&gt;In&lt;/span&gt; summary, for &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; first time, we explored &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; diversity &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; Actinobacteria and evaluated their bioactive potential &lt;span class=&quot;hitHilite&quot;&gt;from&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;Unkeshwar&lt;/span&gt; hot &lt;span class=&quot;hitHilite&quot;&gt;springs&lt;/span&gt;. &lt;span class=&quot;hitHilite&quot;&gt;The&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;potent&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;strains&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;isolated&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; study could be useful &lt;span class=&quot;hitHilite&quot;&gt;in&lt;/span&gt; drug &lt;span class=&quot;hitHilite&quot;&gt;discovery&lt;/span&gt; programs.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">17</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;&lt;span class=&quot;LrzXr kno-fv&quot;&gt;3.049&lt;/span&gt;&lt;/p&gt;
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