<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">SreeHarsha, Nagaraja</style></author><author><style face="normal" font="default" size="100%">Ghorpade, Ravindra, V.</style></author><author><style face="normal" font="default" size="100%">Alzahrani, Abdullah Mossa</style></author><author><style face="normal" font="default" size="100%">Al-Dhubiab, Bandar E.</style></author><author><style face="normal" font="default" size="100%">Venugopala, Katharigatta N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of nanomedicine</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAY</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">3235-3244</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Purpose: Here, we present &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; successful preparation &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;a&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;highly&lt;/span&gt; efficient &lt;span class=&quot;hitHilite&quot;&gt;gallic&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;acid&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;resin grafted&lt;/span&gt; with &lt;span class=&quot;hitHilite&quot;&gt;magnetic&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;nanoparticles&lt;/span&gt; (MNPs) and containing &lt;span class=&quot;hitHilite&quot;&gt;a&lt;/span&gt; branched brush polymeric shell.&lt;br /&gt;
	&lt;br /&gt;
	Methods: &lt;span class=&quot;hitHilite&quot;&gt;Using&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;a&lt;/span&gt; convenient co-precipitation &lt;span class=&quot;hitHilite&quot;&gt;method&lt;/span&gt;, we prepared Fe3O4 &lt;span class=&quot;hitHilite&quot;&gt;nanoparticles&lt;/span&gt; stabilized by citric &lt;span class=&quot;hitHilite&quot;&gt;acid&lt;/span&gt;. These &lt;span class=&quot;hitHilite&quot;&gt;nanoparticles&lt;/span&gt; underwent further silica modification and amino functionalization followed by &lt;span class=&quot;hitHilite&quot;&gt;gallic&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;acid&lt;/span&gt; functionalization &lt;span class=&quot;hitHilite&quot;&gt;on&lt;/span&gt; their surface. Under alkaline conditions, we used &lt;span class=&quot;hitHilite&quot;&gt;a&lt;/span&gt; condensation &lt;span class=&quot;hitHilite&quot;&gt;reaction&lt;/span&gt; that combined formaldehyde and &lt;span class=&quot;hitHilite&quot;&gt;gallic&lt;/span&gt;, &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; graft &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;gallic&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;acid&lt;/span&gt;-formaldehyde resin &lt;span class=&quot;hitHilite&quot;&gt;on&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; surface. We then evaluated &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; polymer-grafted MNPs &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; assay &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;Candida&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;Antarctica&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;B&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;lipase&lt;/span&gt;(Cal-&lt;span class=&quot;hitHilite&quot;&gt;B&lt;/span&gt;) &lt;span class=&quot;hitHilite&quot;&gt;immobilization&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;via&lt;/span&gt; physical adsorption.&lt;br /&gt;
	&lt;br /&gt;
	Conclusion: Furthermore, during optimization &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; parameters that defined conditions &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;immobilization&lt;/span&gt;, we found that &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; optimum &lt;span class=&quot;hitHilite&quot;&gt;immobilization&lt;/span&gt; was achieved in 15 mins Also, optimal &lt;span class=&quot;hitHilite&quot;&gt;immobilization&lt;/span&gt; temperature and pH were 38 degrees C and 7.5, respectively. In addition, &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; reusability study &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; immobilized &lt;span class=&quot;hitHilite&quot;&gt;lipase&lt;/span&gt; polymer-grafted MNPs was done by isolating &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; MNPs from &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;reaction&lt;/span&gt; medium &lt;span class=&quot;hitHilite&quot;&gt;using&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;magnetic&lt;/span&gt; separation, which showed that grafted MNPs reached 5 cycles with 91% activity retention.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;4.471&lt;/p&gt;
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Afzal, Haq Asif</style></author><author><style face="normal" font="default" size="100%">Ghorpade, Ravindra V.</style></author><author><style face="normal" font="default" size="100%">Thorve, Asmita K.</style></author><author><style face="normal" font="default" size="100%">Nagaraja, Sreeharsha</style></author><author><style face="normal" font="default" size="100%">Al-Dhubiab, Bandar E.</style></author><author><style face="normal" font="default" size="100%">Meravanige, Girish</style></author><author><style face="normal" font="default" size="100%">Rasool, Sahibzada Tasleem</style></author><author><style face="normal" font="default" size="100%">Roopashree, Teeka S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Epoxy functionalized polymer grafted magnetic nanoparticles by facile surface initiated polymerization for immobilization studies of Candida Antarctica lipase B</style></title><secondary-title><style face="normal" font="default" size="100%">Reactive &amp; Functional Polymers</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cal-B enzymes</style></keyword><keyword><style  face="normal" font="default" size="100%">enzymes</style></keyword><keyword><style  face="normal" font="default" size="100%">Immoblization</style></keyword><keyword><style  face="normal" font="default" size="100%">Magnetic nanoparticles</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">FEB</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">147</style></volume><pages><style face="normal" font="default" size="100%">104454</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Immobilization of Candida Antarctica lipase B (Cal-B) was done on the epoxy functionalized polymer (EFP) grafted magnetic nanoparticles (MNPs) via covalent attachment with the active epoxy groups. The EFP brushes were grafted on iron-oxide based MNPs by a facile surface-initiated atom transfer radical polymerization (ATRP) using activators generated by electron transfer (AGET) of glycidyl methacrylate (GMA). Each step of the surface modification, polymer grafting, and enzyme immobilization process on the polymer grafted MNPs was studied using Fourier transform infrared spectroscopy (FTIR). A thermogravimetric analysis (TGA) calculated the amount of engineered organic components, a transmission electron microscopy (TEM) visualized the core-shell formation of the MNPs, and a vibrating sample magnetometer (VSM) validated their magnetic properties at various modification stages. The lipase immobilization efficiency was described as a function of immobilization time, as well as, enzyme amount. The activity was characterized within a range of pH, temperature, kinetic parameters, resusability and storage stability, for both the free and immoblized Cal-B enzyme. The results of this study suggested that poly(GMA) grafted MNPs can be successfully used for the immobilization of Cal-B with improved efficiencies compared to those obtained with free soluble lipase. The reported enzyme immobilization method appears to be reproducible and scalable for industrial production.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;3.333&lt;/p&gt;
</style></custom4></record></records></xml>