<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vasilescu, Alina</style></author><author><style face="normal" font="default" size="100%">Ye, Ran</style></author><author><style face="normal" font="default" size="100%">Boulahneche, Samia</style></author><author><style face="normal" font="default" size="100%">Lamraoui, Sabrina</style></author><author><style face="normal" font="default" size="100%">Jijie, Roxana</style></author><author><style face="normal" font="default" size="100%">Medjram, Mohamed Salah</style></author><author><style face="normal" font="default" size="100%">Gaspar, Szilveszter</style></author><author><style face="normal" font="default" size="100%">Singh, Santosh K.</style></author><author><style face="normal" font="default" size="100%">Kurungot, Sreekumar</style></author><author><style face="normal" font="default" size="100%">Melinte, Sorin</style></author><author><style face="normal" font="default" size="100%">Boukherroub, Rabah</style></author><author><style face="normal" font="default" size="100%">Szunerits, Sabine</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 2: Application to the analysis of calcitonin containing pharmaceutical formulation</style></title><secondary-title><style face="normal" font="default" size="100%">Electrochimica Acta</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Calcitonin</style></keyword><keyword><style  face="normal" font="default" size="100%">Disposable electrodes</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrochemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Porous reduced graphene oxide</style></keyword><keyword><style  face="normal" font="default" size="100%">Protein aggregation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR </style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">266</style></volume><pages><style face="normal" font="default" size="100%">364-372</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;In part 1 (A. Vasilescu et al., Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 1: Lysozyme aggregation at pH 2 and 7.4 Electrochem. Acta, 254 (2017) 375 -383) we proposed porous reduced graphene oxide coated glassy carbon electrode (GC/prGO) in combination with differential pulse voltammetry as a new analytical tool for aggregation studies of proteins. Lysozyme was used as a model to follow its aggregation by electrochemical means at pH 2 and pH 7.4, leading to the formation of amyloid and amorphous aggregates, respectively. Part 2 of this work widens the scope of this approach by investigating a biopharmaceutical product, as the aggregation of peptide based drugs affects their therapeutic activity and can induce allergic reactions in patients. The salmon polypeptide calcitonin (sCT) was chosen as an example of a bioactive peptide with limited pharmaceutical potential due to a tendency to form cytotoxic aggregates and amyloid fibrils. For practical applications, screen printed electrodes (SPE) and flexible electrodes (FE) modified with polydiallyldimethylammonium (PDDA) and prGO by using the layer-by-layer deposition technique have been developed for the detection of sCT. The results indicate that these electrodes can differentiate between formation of amyloid aggregates of calcitonin (2 mg mL(-1)) in citrate buffer to no aggregation in acetate buffer. It was further demonstrated that these electrodes are able to analyze a pharmaceutical drug product of low potency, Miacalcic (8.3 mu g mL(-1)), where no aggregation was observed. (C) 2018 Elsevier Ltd. All rights reserved.&lt;/p&gt;</style></abstract><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">4.798</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chekin, Fereshteh</style></author><author><style face="normal" font="default" size="100%">Myshin, Vladyslav</style></author><author><style face="normal" font="default" size="100%">Ye, Ran</style></author><author><style face="normal" font="default" size="100%">Melinte, Sorin</style></author><author><style face="normal" font="default" size="100%">Singh, Santosh K.</style></author><author><style face="normal" font="default" size="100%">Kurungot, Sreekumar</style></author><author><style face="normal" font="default" size="100%">Boukherroub, Rabah</style></author><author><style face="normal" font="default" size="100%">Szunerits, Sabine</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Graphene-modified electrodes for sensing doxorubicin hydrochloride in human plasma</style></title><secondary-title><style face="normal" font="default" size="100%">Analytical and Bioanalytical Chemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Disposable electrodes</style></keyword><keyword><style  face="normal" font="default" size="100%">doxorubicin</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitrogen-doped reduced graphene oxide</style></keyword><keyword><style  face="normal" font="default" size="100%">Serum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">411</style></volume><pages><style face="normal" font="default" size="100%">1509-1516</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Doxorubicin (DOX), an anthracycline molecule, is currently one of the most widely used anticancer drugs in clinics. Systematic treatment of patients with DOX is known to be accompanied by several unpleasant side effects due to the toxicity of the drug. Thus, monitoring of DOX concentration in serum samples has become increasingly important to avoid side effects and ensure therapeutic efficiency. In this study, we discuss the construction of a disposable electrochemical sensor for the direct monitoring of DOX in clinical blood samples. The sensor is based on coating a gold electrode in a flexible integrated electrode construct formed on polyimide sheets using photolithography, with nitrogen-doped reduced graphene oxide (N-rGO) suspended in chitosan. Under optimized conditions, a linear relationship between the oxidative peak current and the concentration of DOX in the range of 0.010-15M with a detection limit of 10nM could be achieved. The sensor was adapted to monitor DOX in serum samples of patients under anticancer treatment.&lt;/p&gt;
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