<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Thakre, Archana</style></author><author><style face="normal" font="default" size="100%">Zore, Gajanan</style></author><author><style face="normal" font="default" size="100%">Kodgire, Santosh</style></author><author><style face="normal" font="default" size="100%">Kazi, Rubina</style></author><author><style face="normal" font="default" size="100%">Mulange, Shradha</style></author><author><style face="normal" font="default" size="100%">Patil, Rajendra</style></author><author><style face="normal" font="default" size="100%">Shelar, Amruta</style></author><author><style face="normal" font="default" size="100%">Santhakumari, Bayitigeri</style></author><author><style face="normal" font="default" size="100%">Kulkarni, Mahesh J.</style></author><author><style face="normal" font="default" size="100%">Kharat, Kiran</style></author><author><style face="normal" font="default" size="100%">Karuppayil, Sankunny Mohan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Limonene inhibits Candida albicans growth by inducing apoptosis</style></title><secondary-title><style face="normal" font="default" size="100%">Medical Mycology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JUL</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">56</style></volume><pages><style face="normal" font="default" size="100%"> 565-578</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Anti-Candida potential of limonene was evaluated against planktonic growth, biofilm (adhesion, development and maturation) and morphogenesis of Candida albicans in this study. Limonene is a major constituent of citrus oil and most frequently used terpene in food and beverage industry due to its pleasant fragrance, nontoxic, and is generally recognized as safe (GRAS) flavoring agent as well as treatment option in many gastrointestinal diseases. Limonene exhibited excellent anti-Candida activity and was equally effective against planktonic growth of C. albicans isolates differentially susceptible to FLC (N = 35). Limonene inhibited morphogenesis significantly at low concentration. However, it showed stage dependent activity against biofilm formation, that is, it was more effective against adhesion followed by development and maturation. Limonene also exhibited excellent synergy with FLC against planktonic and biofilm growth. SWATH-MS analysis led to identification of limonene responsive proteins that provided molecular insight of its anti-Candida activity. Proteomic analysis revealed upregulation of proteins involved in cell wall glucan synthesis (Kre6); oxidative stress (Rhr2, Adh7 and Ebp1); DNA damage stress (Mbf1 and Npl3); nucleolar stress (Rpl11, Rpl7, Rpl29, Rpl15) and down regulation of cytoskeleton organization (Crn1, Pin3, Cct8, Rbl2), and so forth, in response to limonene. Limonene mediated down regulation of Tps3 indicates activation of caspase (CaMca1) and induction of apoptosis in C. albicans. These results suggest that limonene inhibits C. albicans growth by cell wall/membrane damage induced oxidative stress that leads to DNA damage resulting into modulation of cell cycle and induction of apoptosis through nucleolar stress and metacaspase dependent pathway.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;2.799&lt;/p&gt;
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ahmed, Radfan</style></author><author><style face="normal" font="default" size="100%">Kodgire, Santosh</style></author><author><style face="normal" font="default" size="100%">Santhakumari, B.</style></author><author><style face="normal" font="default" size="100%">Patil, Rajendra</style></author><author><style face="normal" font="default" size="100%">Kulkarni, Mahesh J.</style></author><author><style face="normal" font="default" size="100%">Zore, Gajanan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Serum responsive proteome reveals correlation between oxidative phosphorylation and morphogenesis in Candida albicans ATCC10231</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Proteomics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Candida albicans</style></keyword><keyword><style  face="normal" font="default" size="100%">Fetal bovin serum</style></keyword><keyword><style  face="normal" font="default" size="100%">Liquid chromatography mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">morphogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Oxidative phosphorylation</style></keyword><keyword><style  face="normal" font="default" size="100%">pathogenicity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">AUG</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">185</style></volume><pages><style face="normal" font="default" size="100%">25-38</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;To understand the impact of fetal bovine serum (FBS) on metabolism and cellular architecture in addition to morphogenesis, we have identified FBS responsive proteome of Candida albicans. FBS induced 34% hyphae and 60% pseudohyphae in C. albicans at 30 C while 98% hyphae at 37 C. LC-MS/MS analysis revealed that 285 proteins modulated significantly in response to FBS at 30 degrees C and 37 degrees C. Out of which 152 were upregulated and 62 were downregulated at 30 degrees C while 18 were up and 53 were downregulated at 37 degrees C. Functional annotation suggests that FBS may inhibit glycolysis and fermentative pathway and enhance oxidative phosphorylation (OxPhos), TCA cycle, amino acid and fatty acid metabolism indicating a use of alternative energy source by C. albicans. OxPhos inhibition assay using sodium azide corroborated the correlation between inhibition of glycolysis and enhanced OxPhos with pseudohyphae formation. C. albicans induced hyphae in response to FBS irrespective of down regulation of Rasl,Asrl/Asr2, indicates the possible involvement of MAPK and cAMP-PKA independent pathway. The Cell wall of cells grown in presence of FBS at 30 degrees C was rich in mannan, Beta 1,3glucan and chitin while membranes were rich in ergosterol compared to those grown at 37 degrees C. Significance of the study: This is the first study suggesting a correlation between OxPhos and morphogenesis especially pseudohyphae formation in C. albicans. Our data also indicate that fetal bovine serum (FBS) induced morphogenesis is multifactorial and may involve MAPK and cAMP-PKA independent pathway. In addition to morphogenesis, our study provides an insight in to the modulation of metabolism and cellular architecture of C. albicans in response to FBS.&lt;/p&gt;
</style></abstract><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;3.914&lt;/p&gt;
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Zore, Gajanan</style></author><author><style face="normal" font="default" size="100%">Abdulghani, Mazen</style></author><author><style face="normal" font="default" size="100%">Kodgire, Santosh</style></author><author><style face="normal" font="default" size="100%">Kazi, Rubina</style></author><author><style face="normal" font="default" size="100%">Shelar, Amruta</style></author><author><style face="normal" font="default" size="100%">Patil, Rajendra</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Proteome dataset of Candida albicans (ATCC10231) opaque cell</style></title><secondary-title><style face="normal" font="default" size="100%">BMC Research Notes</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JAN</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;h3 class=&quot;c-article__sub-heading&quot; data-test=&quot;abstract-sub-heading&quot; style=&quot;margin: 0px 0px 8px; box-sizing: inherit; font-size: 1.5rem; font-family: -apple-system, BlinkMacSystemFont, &amp;quot;Segoe UI&amp;quot;, Roboto, Oxygen-Sans, Ubuntu, Cantarell, &amp;quot;Helvetica Neue&amp;quot;, sans-serif; line-height: 1.24; font-weight: 400;&quot;&gt;
	Objectives&lt;/h3&gt;
&lt;p style=&quot;margin: 0px; box-sizing: inherit; padding: 0px; overflow-wrap: break-word; word-break: break-word; color: rgb(51, 51, 51); font-family: Georgia, Palatino, serif; font-size: 18px;&quot;&gt;
	&lt;i style=&quot;margin: 0px; box-sizing: inherit;&quot;&gt;Candida albicans&lt;/i&gt;, a polymorphic yeast, is one of the most common, opportunistic fungal pathogens of humans. Among the different morphological forms, opaque form is one of the least-studied ones. This opaque phenotype is essential for mating and is also reported to be involved in colonizing the gastrointestinal tract. Considering the significance of the clinical and sexual reproduction of&amp;nbsp;&lt;i style=&quot;margin: 0px; box-sizing: inherit;&quot;&gt;C. albicans&lt;/i&gt;, we have investigated the morphophysiological modulations in opaque form using a proteomic approach.&lt;/p&gt;
&lt;h3 class=&quot;c-article__sub-heading&quot; data-test=&quot;abstract-sub-heading&quot; style=&quot;margin: 24px 0px 8px; box-sizing: inherit; font-size: 1.5rem; font-family: -apple-system, BlinkMacSystemFont, &amp;quot;Segoe UI&amp;quot;, Roboto, Oxygen-Sans, Ubuntu, Cantarell, &amp;quot;Helvetica Neue&amp;quot;, sans-serif; line-height: 1.24; font-weight: 400;&quot;&gt;
	Data description&lt;/h3&gt;
&lt;p style=&quot;margin: 0px; box-sizing: inherit; padding: 0px; overflow-wrap: break-word; word-break: break-word; color: rgb(51, 51, 51); font-family: Georgia, Palatino, serif; font-size: 18px;&quot;&gt;
	In the current investigation, we have used Micro-Liquid Chromatography-Mass Spectrometry (LC-MS/MS) analysis to create a protein profile for opaque-specific proteins. Whole-cell proteins from&amp;nbsp;&lt;i style=&quot;margin: 0px; box-sizing: inherit;&quot;&gt;C. albicans&lt;/i&gt;&amp;nbsp;(ATCC10231) cells that had been cultured for seven days on synthetic complete dextrose (SCD) medium in both as an opaque (test) and as a white (control) form cells were extracted, digested, and identified using LC-MS/MS. This information is meant to serve the scientific community and represents the proteome profile (SWATH Spectral Libraries) of&amp;nbsp;&lt;i style=&quot;margin: 0px; box-sizing: inherit;&quot;&gt;C. albicans&lt;/i&gt;&amp;nbsp;opaque form.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;
	Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;
	1.6&lt;/p&gt;
</style></custom4></record></records></xml>