<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Yadav, Sagar</style></author><author><style face="normal" font="default" size="100%">Maiti, Saborni</style></author><author><style face="normal" font="default" size="100%">Mundhe, Swapnil</style></author><author><style face="normal" font="default" size="100%">Kadoo, Narendra</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Metabolomic profiling unravels the role of sphingolipid pathways in spot blotch resistance in wheat</style></title><secondary-title><style face="normal" font="default" size="100%">Acta Physiologiae Plantarum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cochliobolus sativus</style></keyword><keyword><style  face="normal" font="default" size="100%">High-resolution mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">LC-HRMS</style></keyword><keyword><style  face="normal" font="default" size="100%">Metabolite profiling</style></keyword><keyword><style  face="normal" font="default" size="100%">OPLS-DA</style></keyword><keyword><style  face="normal" font="default" size="100%">plant-pathogen interaction</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">JUN</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">47</style></volume><pages><style face="normal" font="default" size="100%">67</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;
	Spot blotch, caused by the soil- and air-borne fungal pathogen Bipolaris sorokiniana, is a major threat to wheat production. The disease is reaching epidemic proportions in wheat-growing areas, particularly in South Asia, South America, Africa, and Australia. In India, over 25 million hectares of wheat-growing area is threatened by this disease. A systematic study of metabolites can provide insights into the molecular basis of this disease. In the present study, we evaluated the impact of B. sorokiniana inoculation on two wheat varieties, Chirya3 (resistant to spot blotch) and DDK1025 (susceptible to spot blotch). We performed time-course non-targeted metabolite profiling of the pathogen-inoculated and mock-inoculated plants using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS). Multivariate analysis was performed to construct a comprehensive statistical workflow, which led to the defined ``metabolomic phenotypes''. Modeling by Orthogonal Projection to Latent Structures-Discriminant Analysis (OPLS-DA) revealed significant metabolites in responses of the resistant and susceptible varieties to pathogen inoculation. A total of 699 metabolites displayed significant variations during the progression of infection. B. sorokiniana-inoculated Chirya3 exhibited high levels of some metabolites, such as sphingolipids, cysteine, phenylalanine, shikimates, etc. The study revealed that sphingolipid pathways are critical in resistance mechanisms contributing to enhanced lignification and disease resistance in wheat.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;
	Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;
	2.8&lt;/p&gt;
</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Salunke, Gayatri</style></author><author><style face="normal" font="default" size="100%">Badhe, Yogesh</style></author><author><style face="normal" font="default" size="100%">Singh, Vrijendra</style></author><author><style face="normal" font="default" size="100%">Ghorpade, Prakash</style></author><author><style face="normal" font="default" size="100%">Hegde, Mahabaleshwar</style></author><author><style face="normal" font="default" size="100%">Kadoo, Narendra</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Molecular insights into the oleic acid accumulation in safflower</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of the American Oil Chemists Society</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Carthamus tinctorius</style></keyword><keyword><style  face="normal" font="default" size="100%">FAD2</style></keyword><keyword><style  face="normal" font="default" size="100%">FATB</style></keyword><keyword><style  face="normal" font="default" size="100%">Fatty acid desaturase</style></keyword><keyword><style  face="normal" font="default" size="100%">fatty acid thioesterase</style></keyword><keyword><style  face="normal" font="default" size="100%">linoleic acid</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">FEB</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">102</style></volume><pages><style face="normal" font="default" size="100%">351-363</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;
	Most of the Indian safflower (Carthamus tinctorius L.) varieties produce oil rich in linoleic acid (LA, similar to 75%) and low in oleic acid (OA, similar to 15%). In the fatty acid biosynthetic pathway, the fatty acid desaturase 2 (FAD2) enzyme converts OA to LA. Safflower is reported to have 12-20 FAD2 genes. Gene expression analysis of four FAD2 genes during seed development in a high LA variety, PBNS-12, revealed high expression of FAD2-1 at 21 days after flowering (DAF), correlating with high LA accumulation. Fatty acid profiling of 448 Indian safflower germplasm accessions revealed four lines to have high (58%-77%) OA content, with NASF-39 having the highest OA content. Interestingly, all four high OA lines showed the same mutation in the FAD2-1 gene. The DNA sequence of FAD2-1 from the four high OA lines showed a deletion of C at the +606 position, resulting in a premature stop codon at the +733 position and a truncated protein of 244 amino acids. Hence, despite the high expression levels of FAD2-1 in NASF-39 at 18-21 DAF, it exhibited high OA (77%). The dysfunctional nature of the truncated FAD2-1 in NASF-39 was evident in molecular docking studies with 1-stearoyl-2-oleoyl phosphatidylcholine. We also sequenced FATB, a thioesterase responsible for releasing stearic acid from acyl carrier protein for further desaturation to oleic acid, where an A773G substitution was observed. This resulted in E258G substitution in NASF-39 FATB compared to that of PBNS-12. This probably made the acyl-binding pocket of NASF-39 FATB unstable, contributing to high OA accumulation. Thus, the outcomes of this study can help develop super and ultra-high oleic safflower varieties through various genetics and genomics approaches.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;
	Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;
	1.9&lt;/p&gt;
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