<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Verma, Ashish</style></author><author><style face="normal" font="default" size="100%">Pal, Yash</style></author><author><style face="normal" font="default" size="100%">Khatri, Indu</style></author><author><style face="normal" font="default" size="100%">Ojha, Anup Kumar</style></author><author><style face="normal" font="default" size="100%">Gruber-Vodicka, Harald</style></author><author><style face="normal" font="default" size="100%">Schumann, Peter</style></author><author><style face="normal" font="default" size="100%">Dastager, Syed Gulam</style></author><author><style face="normal" font="default" size="100%">Subramanian, Srikrishna</style></author><author><style face="normal" font="default" size="100%">Mayilraj, Shanmugam</style></author><author><style face="normal" font="default" size="100%">Krishnamurthi, Srinivasan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Examination into the taxonomic position of Bacillus thermotolerans Yang et al., 2013, proposal for its reclassification into a new genus and species Quasibacillus thermotolerans gen. nov., comb. nov. and reclassification of B. encimensis Dastager et al., </style></title><secondary-title><style face="normal" font="default" size="100%">Systematic and Applied Microbiology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">SEP</style></date></pub-dates></dates><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Two novel Gram-staining positive, rod-shaped, moderately halotolerant, endospore forming bacterial strains 5.5LF 38TD and 5.5LF 48TD were isolated and taxonomically characterized from a landfill in Chandigarh, India. The analysis of 16S rRNA gene sequences of the strains confirmed their closest identity to Bacillus thermotolerans SgZ-8T with 99.9% sequence similarity. A comparative phylogenetic analysis of strains 5.5LF 38TD, 5.5LF 48TD and B. thermotolerans SgZ-8(T) confirmed their separation into a novel genus with B. badius and genus Domibacillus as the closest phylogenetic relatives. The major fatty acids of the strains are iso-C15:0 and iso-C16:0 and MK-7 is the only quinone. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The digital DNA-DNA hybridization (DDH) and ortho average nucleotide identity (ANI) values calculated through whole genome sequences indicated that the three strains showed low relatedness with their phylogenetic neighbours. Based on evidences from phylogenomic analyses and polyphasic taxonomic characterization we propose reclassification of the species B. thermotolerans into a novel genus named Quasibacillus thermotolerans gen. nov., comb. nov with the type strain SgZ-8(T) (=CCTCC AB2012108(T)=KACC 16706(T)). Further our analyses also revealed that B. encimensis SGD-V-25(T) is a later heterotypic synonym of Bacillus badius DSM 23(T).</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">3.691</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Verma, Ashish</style></author><author><style face="normal" font="default" size="100%">Pal, Yash</style></author><author><style face="normal" font="default" size="100%">Ojha, Anup Kumar</style></author><author><style face="normal" font="default" size="100%">Kumari, Munesh</style></author><author><style face="normal" font="default" size="100%">Khatri, Indu</style></author><author><style face="normal" font="default" size="100%">Rameshkumar, N.</style></author><author><style face="normal" font="default" size="100%">Schumann, Peter</style></author><author><style face="normal" font="default" size="100%">Dastager, Syed G.</style></author><author><style face="normal" font="default" size="100%">Mayilraj, Shanmugam</style></author><author><style face="normal" font="default" size="100%">Subramanian, Srikrishna</style></author><author><style face="normal" font="default" size="100%">Krishnamurthi, Srinivasan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Taxonomic insights into the phylogeny of Bacillus badius and proposal for its reclassification to the genus Pseudobacillus as Pseudobacillus badius comb. nov. and reclassification of Bacillus wudalianchiensis Liu et al.</style></title><secondary-title><style face="normal" font="default" size="100%">Systematic and Applied Microbiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">16S rRNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacillus</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacillus wudalianchiensis</style></keyword><keyword><style  face="normal" font="default" size="100%">Fatty acids</style></keyword><keyword><style  face="normal" font="default" size="100%">polar lipids</style></keyword><keyword><style  face="normal" font="default" size="100%">Pseudobacillus</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAY</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">42</style></volume><pages><style face="normal" font="default" size="100%">360-372</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The species Bacillus badius is one of the oldest members of the genus Bacillus isolated from faeces of children and was classified based on its ability to form endospores [8]. In 16S rRNA gene sequence and phylogenetic analysis, Bacillus badius DSM 23(T) shared low similarity (93.0%) and distant relationship with B. subtilis, the type species of the genus Bacillus indicating that it does not belong to this genus. Additional strains of the species, B. badius DSM 5610, DSM 30822 and B. encimensis SGD-V-25 (which has been recently reclassified as a member of this species) were included in the study to consider intraspecies diversity. Detailed molecular phylogenetic and comparative genome analysis clearly showed that the strains of B. badius were consistently retrieved outside the cluster of Bacillus sensu stricto and also distantly related to the genera Domibacillus and Quasibacillus. Further, the data from biochemical reactions (inability to ferment most carbohydrates), polar lipids profile (presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an aminophosphoglycolipid) and fatty acids supported the molecular analysis. Thus the four B. badius strains; DSM 23(T), DSM 5610, DSM 30822 and SGD-V-25 displayed sufficient demarcating phenotypic characteristics that warrant their classification as members of a novel genus and single species, for which the name Pseudobacillus badius gen. nov. comb. nov. is proposed with Pseudobacillus badius DSM 23(T) (= ATCC 14574(T)) as the type strain. Additionally, based on our findings from phenotypic, chemotaxonomic and genotypic parameters, Bacillus wudalianchiensis DSM 100757(T) was reclassified as Pseudobacillus wudalianchiensis comb. nov. (C) 2019 Elsevier GmbH. All rights reserved.&lt;/p&gt;
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