<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">SreeHarsha, Nagaraja</style></author><author><style face="normal" font="default" size="100%">Ghorpade, Ravindra, V.</style></author><author><style face="normal" font="default" size="100%">Alzahrani, Abdullah Mossa</style></author><author><style face="normal" font="default" size="100%">Al-Dhubiab, Bandar E.</style></author><author><style face="normal" font="default" size="100%">Venugopala, Katharigatta N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of nanomedicine</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAY</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">3235-3244</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Purpose: Here, we present &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; successful preparation &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;a&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;highly&lt;/span&gt; efficient &lt;span class=&quot;hitHilite&quot;&gt;gallic&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;acid&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;resin grafted&lt;/span&gt; with &lt;span class=&quot;hitHilite&quot;&gt;magnetic&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;nanoparticles&lt;/span&gt; (MNPs) and containing &lt;span class=&quot;hitHilite&quot;&gt;a&lt;/span&gt; branched brush polymeric shell.&lt;br /&gt;
	&lt;br /&gt;
	Methods: &lt;span class=&quot;hitHilite&quot;&gt;Using&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;a&lt;/span&gt; convenient co-precipitation &lt;span class=&quot;hitHilite&quot;&gt;method&lt;/span&gt;, we prepared Fe3O4 &lt;span class=&quot;hitHilite&quot;&gt;nanoparticles&lt;/span&gt; stabilized by citric &lt;span class=&quot;hitHilite&quot;&gt;acid&lt;/span&gt;. These &lt;span class=&quot;hitHilite&quot;&gt;nanoparticles&lt;/span&gt; underwent further silica modification and amino functionalization followed by &lt;span class=&quot;hitHilite&quot;&gt;gallic&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;acid&lt;/span&gt; functionalization &lt;span class=&quot;hitHilite&quot;&gt;on&lt;/span&gt; their surface. Under alkaline conditions, we used &lt;span class=&quot;hitHilite&quot;&gt;a&lt;/span&gt; condensation &lt;span class=&quot;hitHilite&quot;&gt;reaction&lt;/span&gt; that combined formaldehyde and &lt;span class=&quot;hitHilite&quot;&gt;gallic&lt;/span&gt;, &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; graft &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;gallic&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;acid&lt;/span&gt;-formaldehyde resin &lt;span class=&quot;hitHilite&quot;&gt;on&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; surface. We then evaluated &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; polymer-grafted MNPs &lt;span class=&quot;hitHilite&quot;&gt;to&lt;/span&gt; assay &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;Candida&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;Antarctica&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;B&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;lipase&lt;/span&gt;(Cal-&lt;span class=&quot;hitHilite&quot;&gt;B&lt;/span&gt;) &lt;span class=&quot;hitHilite&quot;&gt;immobilization&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;via&lt;/span&gt; physical adsorption.&lt;br /&gt;
	&lt;br /&gt;
	Conclusion: Furthermore, during optimization &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; parameters that defined conditions &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;immobilization&lt;/span&gt;, we found that &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; optimum &lt;span class=&quot;hitHilite&quot;&gt;immobilization&lt;/span&gt; was achieved in 15 mins Also, optimal &lt;span class=&quot;hitHilite&quot;&gt;immobilization&lt;/span&gt; temperature and pH were 38 degrees C and 7.5, respectively. In addition, &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; reusability study &lt;span class=&quot;hitHilite&quot;&gt;of&lt;/span&gt; immobilized &lt;span class=&quot;hitHilite&quot;&gt;lipase&lt;/span&gt; polymer-grafted MNPs was done by isolating &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; MNPs from &lt;span class=&quot;hitHilite&quot;&gt;the&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;reaction&lt;/span&gt; medium &lt;span class=&quot;hitHilite&quot;&gt;using&lt;/span&gt; &lt;span class=&quot;hitHilite&quot;&gt;magnetic&lt;/span&gt; separation, which showed that grafted MNPs reached 5 cycles with 91% activity retention.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;4.471&lt;/p&gt;
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