<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Talekar, Sachin</style></author><author><style face="normal" font="default" size="100%">Pandharbale, Amol</style></author><author><style face="normal" font="default" size="100%">Ladole, Mayur</style></author><author><style face="normal" font="default" size="100%">Nadar, Shamraja</style></author><author><style face="normal" font="default" size="100%">Mulla, Mosin</style></author><author><style face="normal" font="default" size="100%">Japhalekar, Kshitija</style></author><author><style face="normal" font="default" size="100%">Pattankude, Kishori</style></author><author><style face="normal" font="default" size="100%">Arage, Devika</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Carrier free co-immobilization of alpha amylase, glucoamylase and pullulanase as combined cross-linked enzyme aggregates (combi-CLEAs): a tri-enzyme biocatalyst with one pot starch hydrolytic activity</style></title><secondary-title><style face="normal" font="default" size="100%">Bioresource Technology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alpha amylase</style></keyword><keyword><style  face="normal" font="default" size="100%">Combined cross-linked enzyme aggregates (combi-CLEAs)</style></keyword><keyword><style  face="normal" font="default" size="100%">Glucoamylase</style></keyword><keyword><style  face="normal" font="default" size="100%">One pot starch hydrolysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Pullulanase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">NOV</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">ELSEVIER SCI LTD</style></publisher><pub-location><style face="normal" font="default" size="100%">THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND</style></pub-location><volume><style face="normal" font="default" size="100%">147</style></volume><pages><style face="normal" font="default" size="100%">269-275</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A tri-enzyme biocatalyst ``combi-CLEAs'' with starch hydrolytic activity was prepared from commercially available alpha amylase, glucoamylase and pullulanase preparations by aggregating enzymes with ammonium sulphate followed by cross-linking formed aggregates for 4.5 h with 40 mM glutaraldehyde. The effects of precipitant type and cross-linking were studied and the biocatalyst was characterized. Scanning electron microscopy analysis showed that tri-enzyme biocatalyst was of spherical structure. For one pot starch hydrolytic activity, shift in optimum pH from 6 to 7 and temperature from 65 to 75 degrees C were observed after co-immobilization of enzymes. After one pot starch hydrolysis reaction in batch mode, 100%, 60% and 40% conversions were obtained with combi-CLEAs, separate CLEAs mixture and free enzyme mixture, respectively. Co-immobilization also enhanced the thermal stability of enzymes. Finally, the catalytic activity of enzymes in combi-CLEAs during one pot starch hydrolysis was well maintained up to five cycles without performance changes. (C) 2013 Elsevier Ltd. All rights reserved.&lt;/p&gt;</style></abstract><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">5.039
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