00882nas a2200145 4500008004100000022001400041245011200055210006900167260011000236300001200346520021700358100001900575700001400594856012800608 2005 eng d a1359-734500aBackbone-extended pyrrolidine peptide nucleic acids (bepPNA): design, synthesis and DNA/RNA binding studies0 aBackboneextended pyrrolidine peptide nucleic acids bepPNA design aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJAN a495-4973 a

One-carbon extended conformationally constrained pyrrolidine PNA monomer (bepPNA) has been synthesized, incorporated into PNA sequences at predefined positions, and showed selective RNA binding properties.

1 aGovindaraju, T1 aKumar, VA uhttps://library.ncl.res.in/content/backbone-extended-pyrrolidine-peptide-nucleic-acids-beppna-design-synthesis-and-dnarna-000679nas a2200193 4500008004100000022001400041245006100055210006100116260007100177300001400248490000800262100002100270700001300291700001600304700002500320700003000345700001500375856009500390 2005 eng d a0002-786300aBacterial aerobic synthesis of nanocrystalline magnetite0 aBacterial aerobic synthesis of nanocrystalline magnetite a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcJUL a9326-93270 v1271 aBharde, Atul, A.1 aWani, A.1 aShouche, Y.1 aJoy, Pattayil, Alias1 aPrasad, Bhagavatula L. V.1 aSastry, M. uhttps://library.ncl.res.in/content/bacterial-aerobic-synthesis-nanocrystalline-magnetite-002591nas a2200241 4500008004100000022001400041245020000055210006900255260007700324300001400401490000700415520160100422653001902023653001302042653002602055653002602081653002402107100001902131700002402150700002302174700002502197856012702222 2005 eng d a0947-653900aBenzoyl transfer reactivities of racemic 2,4-Di-O-acyl-myo-inosityl 1,3,5-orthoesters in the solid state: molecular packing and intermolecular interactions correlate with the ease of the reaction0 aBenzoyl transfer reactivities of racemic 24DiOacylmyoinosityl 13 aPO BOX 10 11 61, D-69451 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcMAR a2103-21100 v113 a

Racemic 2,4-di-O-acyl-myoinosityl 1,3,5-orthoesters undergo transesterification catalyzed by sodium carbonate with varying ease of reaction in the solid state; reactions in solution and melt do not show such varied differences. An interesting crystal of a 1:1 molecular complex of highly reactive racemic 2,4-di-O-benzoyl-myo-inosityl 1,3,5-orthoformate and its orthoacetate analogue exhibited better reactivity than the latter component alone. Single-crystal X-ray structures of the reactants have been correlated with the observed differences in the acyl-transfer efficiencies in the solid state. Although each of the derivatives helically self-assembles around the crystallographic 2(1) axis linked through O-(HO)-O-... hydrogen bonding, the pre-organization of the reactive groups (C=O [El] and OH [Nu]), C-(HO)-O-... and the C=H(...)pi interactions are significantly more favourable for the reactive derivatives than the less reactive ones. Bond-length distributions also showed differences; the O-C bond of the axial benzoyl group, which gets cleaved during the reaction, is longer (1.345-1.361 angstrom) relative to the chemically equivalent O-C bond of the equatorial benzoyl group (1.316-1.344 angstrom) in the reactive derivatives. These bond-length differences are not significant in the less reactive derivatives. The overall molecular organization is different too; the strikingly discrete helices, which may be viewed as ``reaction tunnels'' and are held by interhelical interactions, are clearly evident in the reactive derivatives in comparison with the less reactive ones.

10ahydrogen bonds10aInositol10asolid-state reactions10astructure elucidation10atransesterification1 aSarmah, M., P.1 aGonnade, Rajesh, G.1 aShashidhar, M., S.1 aBhadbhade, Mohan, M. uhttps://library.ncl.res.in/content/benzoyl-transfer-reactivities-racemic-24-di-o-acyl-myo-inosityl-135-orthoesters-solid-102012nas a2200229 4500008004100000022001400041245009400055210006900149260010600218300001400324490000700338520114600345653001701491653002101508653002401529653001601553100002201569700002201591700001901613700002301632856012701655 2005 eng d a0014-305700aBenzoylation of polyphenylene oxide: characterization and gas permeability investigations0 aBenzoylation of polyphenylene oxide characterization and gas per aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcOCT a2461-24710 v413 a

Benzoylation of polyphenylene oxide (PPO) was carried out with aromatic acid chlorides bearing specific groups at para-position (H methyl, Br, Cl and nitro), which differ in their polarity and bulk. The reaction conditions were optimized individually to get the high degree of substitution. These materials were characterized for thermal as well as other physical properties that are known to affect the gas permeation. In a series investigated, the nitrobenzoyl substitution on PPO resulted in the highest increase in glass transition temperature and the lowest thermal stability. An estimation of the packing density parameters-fractional free volume by density measurement and the d-spacing by X-ray diffraction analysis showed an increase in the packing density. The gas permeability was found to decrease in all the cases of benzoylation. The helium and oxygen based selectivities were increased, while CO2 based selectivities were decreased. The unusual trend observed in the gas permeation properties is explained on the basis of nature of substituent and the degree of substitution. (c) 2005 Elsevier Ltd. All rights reserved.

10abenzoylation10aGas permeability10apolyphenylene oxide10aselectivity1 aBhole, Yogesh, S.1 aKharul, Ulhas, K.1 aSomani, S., P.1 aKumbharkar, S., C. uhttps://library.ncl.res.in/content/benzoylation-polyphenylene-oxide-characterization-and-gas-permeability-investigations-002262nas a2200241 4500008004100000022001400041245010100055210006900156260007300225300001200298490000700310520141400317653001501731653001301746653001601759653001101775100001301786700001901799700002101818700002101839700002801860856013201888 2005 eng d a1387-181100aBenzylation of benzene and other aromatics by benzyl chloride over mesoporous AlSBA-15 catalysts0 aBenzylation of benzene and other aromatics by benzyl chloride ov aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAY a195-2030 v803 a

Aluminum-containing mesoporous molecular sieves AISBA-15 with different n(Si)/n(Al), ratios and AIMCM-41 have been synthesized hydrothermally and characterized in detail by physicochemical methods, viz. XRD, N(2) adsorption and (27)Al NMR spectroscopy. The low angle XRD and N2 adsorption measurements reveal that the structural order of SBA-15 was retained after the incorporation of Al. The increase of the unit cell parameter with increasing aluminum content and (27)Al MAS NMR spectroscopy confirm the incorporation of aluminum in the framework. Benzylation of benzene and substituted benzenes reaction employing benzyl chloride as the alkylating agent over AISBA-15 and AIMCM-41 have been investigated. The influence of various reaction parameters such as reaction temperature, reactant feed ratio and catalyst amount affecting the activity and selectivity of AISBA-15, have been studied. Among the mesoporous catalysts studied, AISBA-15(45), where the number in parentheses indicates the molar nsilnA, ratio, shows both high conversion and high selectivity for the benzylation of benzene. The activity of this catalyst for the benzylation of different aromatic compounds is in the following order: benzene > toluene > p-xylene > mesitylene > anisole. Kinetics of the benzene benzylation over different catalysts have also been investigated. (c) 2005 Elsevier Inc. All rights reserved.

10aAlkylation10aAlSBA-1510aBenzylation10aSBA-151 aVinu, A.1 aSawant, D., P.1 aAriga, Katsuhiko1 aHartmann, Martin1 aHalligudi, Shivappa, B. uhttps://library.ncl.res.in/content/benzylation-benzene-and-other-aromatics-benzyl-chloride-over-mesoporous-alsba-15-catalysts-002056nas a2200205 4500008004100000022001400041245012600055210006900181260007100250300001600321490000700337520125700344100001701601700001801618700002401636700001601660700002301676700001901699856013201718 2005 eng d a0743-746300aBiocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview0 aBiocompatibility of gold nanoparticles and their endocytotic fat a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcNOV a10644-106540 v213 a

Macrophages are one of the principal immune effector cells that play essential roles as secretory, phagocytic, and antigen-presenting cells in the immune system. In this study, we address the issue of cytotoxicity and immunogenic effects of gold nanoparticles on RAW264.7 macrophage cells. The cytotoxicity of gold nanoparticles has been correlated with a detailed study of their endocytotic uptake using various microscopy tools such as atomic force microscopy (AFM), confocal-laser-scanning microscopy (CFLSM), and transmission electron microscopy (TEM). Our findings suggest that Au(0) nanoparticles are not cytotoxic, reduce the production of reactive oxygen and nitrite species, and do not elicit secretion of proinflammatory cytokines TNF-alpha and IL1-, making them suitable candidates for nanomedicine. AFM measurements suggest that gold nanoparticles are internalized inside the cell via a mechanism involving pinocytosis, while CFLSM and TEM studies indicate their internalization in lysosomal bodies arranged in perinuclear fashion. Our studies thus underline the noncytotoxic, nonimmunogenic, and biocompatible properties ofgold nanoparticles with the potential for application in nanoimmunology, nanomedicine, and nanobiotechnology.

1 aShukla, Ravi1 aBansal, Vipul1 aChaudhary, Manakshi1 aBasu, Atanu1 aBhonde, Ramesh, R.1 aSastry, Murali uhttps://library.ncl.res.in/content/biocompatibility-gold-nanoparticles-and-their-endocytotic-fate-inside-cellular-compartment-001154nas a2200193 4500008004100000022001400041245011600055210006900171260007700240300000900317490000700326520042000333100001800753700001500771700001700786700001700803700001900820856012100839 2005 eng d a0935-964800aBioleaching of sand by the fungus fusarium oxysporum as a means of producing extracellular silica nanoparticles0 aBioleaching of sand by the fungus fusarium oxysporum as a means  aPO BOX 10 11 61, D-69451 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcAPR a889+0 v173 a

A plant pathogenic fungus, Fusarium oxysporum, can be used as a biological model system for the extracellular bioleaching of hollow spherical silica nanoparticles (see Figure) from sand. The room-temperature synthesis of oxide nanomaterials using microorganisms starting from potential waste materials could lead to eco-friendly and economically viable methods for the large-scale synthesis of nanomaterials.

1 aBansal, Vipul1 aSanyal, A.1 aRautaray, D.1 aAhmad, Absar1 aSastry, Murali uhttps://library.ncl.res.in/content/bioleaching-sand-fungus-fusarium-oxysporum-means-producing-extracellular-silica-001229nas a2200193 4500008004100000022001400041245011700055210006900172260007100241300001200312490000600324520049000330100001700820700001500837700002100852700001700873700001500890856013000905 2005 eng d a1528-748300aBiological synthesis of stable vaterite crystals by the reaction of calcium ions with germinating chickpea seeds0 aBiological synthesis of stable vaterite crystals by the reaction a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcMAR a399-4020 v53 a

The total biological synthesis of CaCO3 crystals of the metastable vaterite polymorph at room temperature by simple protein-mediated reaction of aqueous Ca2+ ions with the CO2 generated during the root growth from germinating chickpea (Cicer arietinum) seeds is described. Highly stable vaterite crystals of spherical morphology are obtained, indicating that the proteins secreted from the roots during growth play a crucial role in stabilizing and directing the crystal growth.

1 aRautaray, D.1 aSanyal, A.1 aBharde, Atul, A.1 aAhmad, Absar1 aSastry, M. uhttps://library.ncl.res.in/content/biological-synthesis-stable-vaterite-crystals-reaction-calcium-ions-germinating-chickpea-002139nas a2200229 4500008004100000022001400041245015600055210006900211260009200280300001400372490000600386520124100392653001701633653001801650653001901668653002101687100002501708700001401733700001701747700001501764856013001779 2005 eng d a1533-488000aBiosynthesis of gold and silver nanoparticles using emblica officinalis fruit extract, their phase transfer and transmetallation in an organic solution0 aBiosynthesis of gold and silver nanoparticles using emblica offi a26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USAbAMER SCIENTIFIC PUBLISHERScOCT a1665-16710 v53 a

The design, synthesis and characterization of biologically synthesized nanomaterials have become an area of significant interest. In this paper, we report the extracellular synthesis of gold and silver nanoparticles using Emblica Officinalis (amla, Indian Gooseberry) fruit extract as the reducing agent to synthesize Ag and Au nanoparticles, their subsequent phase transfer to an organic solution and the transmetallation reaction of hydrophobized silver nanoparticles with hydrophobized chloroaurate ions. On treating aqueous silver sulfate and chloroauric acid solutions with Emblica Officinalis fruit extract, rapid reduction of the silver and chloroaurate ions is observed leading to the formation of highly stable silver and gold nanoparticles in solution. Transmission Electron Microscopy analysis of the silver and gold nanoparticles indicated that they ranged in size from 10 to 20 nm and 15 to 25 nm respectively. Ag and Au nanoparticles thus synthesized were then phase transferred into an organic solution using a cationic surfactant octadecylamine. Transmetallation reaction between hydrophobized silver nanoparticles and hydrophobized chloroaurate ions in chloroform resulted in the formation of gold nanoparticles.

10aBiosynthesis10aNanomaterials10aPhase transfer10aTransmetallation1 aAnkamwar, Balaprasad1 aDamle, C.1 aAhmad, Absar1 aSastry, M. uhttps://library.ncl.res.in/content/biosynthesis-gold-and-silver-nanoparticles-using-emblica-officinalis-fruit-extract-their-001797nas a2200265 4500008004100000022001400041245011200055210006900167260007300236300001000309490000800319520082700327653001701154653001301171653004001184653004201224653002101266653001801287653003301305100002201338700001701360700001301377700001501390856012601405 2005 eng d a0926-860X00aBiphenyl hydrogenation over supported transition metal catalysts under supercritical carbon dioxide solvent0 aBiphenyl hydrogenation over supported transition metal catalysts aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUL a43-470 v2883 a

Catalytic hydrogenation of biphenyl to bicyclohexyl, an organic hydrogen storage medium, was examined over supported transition metal catalysts in supercritical carbon dioxide solvent. The yield of bicyclohexyl was almost 100% over the charcoal-supported rhodium (Rh/C) and ruthenium (Ru/C) catalysts at the temperature of 323 K, which was much lower than that required for biphenyl hydrogenation in organic solvents (573 K). The initial activity was higher over the Rh/C catalyst, while the initial selectivity to bicyclohexyl was higher over the Ru/C catalyst. The conversion of biphenyl increased with increase in hydrogen and carbon dioxide pressures, while the selectivity to bicyclohexyl was independent of hydrogen and carbon dioxide pressures over both catalysts. (c) 2005 Elsevier B.V. All rights reserved.

10abicyclohexyl10abiphenyl10acharcoal-supported rhodium catalyst10acharcoal-supported ruthenium catalyst10aHydrogen storage10aHydrogenation10aSupercritical carbon dioxide1 aHiyoshi, Norihito1 aRode, C., V.1 aSato, O.1 aShirai, M. uhttps://library.ncl.res.in/content/biphenyl-hydrogenation-over-supported-transition-metal-catalysts-under-supercritical-002326nas a2200241 4500008004100000022001400041245018300055210006900238260007300307300001200380490000800392520131100400653001801711653002901729653005701758653003301815653002301848100002301871700001701894700001901911700002801930856012601958 2005 eng d a1381-116900aBis(2-[alpha-hydroxyethyl]benzimidazolato)copper(II) anchored onto chloromethylated polystyrene for the biomimetic oxidative coupling of 2-aminophenol to 2-aminophenoxazine-3-one0 aBis2alphahydroxyethylbenzimidazolatocopperII anchored onto chlor aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUL a132-1380 v2363 a

2-(alpha-Hydroxyethyl)benzimidazole (Hhebmz) has been covalently anchored to chloromethylated polystyrene cross-linked with 5% divinylbenzene. This polymer-anchored chelating resin reacts with cupric acetate in presence of Hhebmz to give polymer-anchored copper(II) complex PS-[Cu(hebmz)(2)]. Elemental analyses, FT-IR, electronic, scanning electron micrographs (SEM) and TGA-DTA pattern have been used to characterize the complex. Oxidative coupling of 2-aminophenol (OAP) to 2-aminophenoxazine-3-one (APX) catalyzed by PS-[Cu(hebmz)(2)] in presence of air at 70 degrees C in DMF gave 62% substrate conversion over a period of 8 h. Kinetics of the above reaction indicated that the rate of oxidative coupling of OAP has a first order dependence with respect to substrate, catalyst and air pressure (dissolved O-2) concentrations in the range of conditions evaluated. Based on these observations, a plausible mechanism for the above reaction has been proposed. From, the study on the effect of temperature on the rates of oxidative coupling, an activation energy 34.2 kcal/degree mole was evaluated from Arrhenius plot of -ln rate versus 1/T. Enthalpy, entropy and free energy were calculated using standard thermodynamic equations for the above reaction. (c) 2005 Elsevier B.V. All rights reserved.

10a2-aminophenol10a2-aminophenoxazine-3-one10abis(2-[alpha-hydroxyethyl]benzimidazolato)copper(II)10achloromethylated polystyrene10aoxidative coupling1 aMaurya, Mannar, R.1 aSikarwar, S.1 aJoseph, Trissa1 aHalligudi, Shivappa, B. uhttps://library.ncl.res.in/content/bis2-alpha-hydroxyethylbenzimidazolatocopperii-anchored-chloromethylated-polystyrene-101799nas a2200217 4500008004100000022001400041245013000055210006900185260007300254300001200327490000800339520095800347653001001305653003201315653002701347653001701374100001901391700001401410700002801424856012901452 2005 eng d a1381-116900aBronsted acidic ionic liquids: a green, efficient and reusable catalyst system and reaction medium for fischer esterification0 aBronsted acidic ionic liquids a green efficient and reusable cat aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUN a107-1100 v2343 a

Bronsted acidic ionic liquid containing nitrogen-based organic cations 1-methylimidazole and 1-butyl-3-methylimidazolium and inorganic anions of the type BF4-, PF6- and PTSA(-) has been synthesized in good yields and used as a catalysts and reaction medium for Fischer esterification of alcohols with acids. These were characterized using FTIR and NMR spectroscopy. Ionic liquids as catalyst afforded good alcohol conversion and excellent ester selectivity. Fischer esterification of acetic acid with benzyl alcohol with different Bronsted acidic ionic liquids was carried out. Maximum substrate conversion 100 % and product selectivity 100 % was observed on using [BMIM](+)PTSAas catalyst over a period of 2 h. The ester was easily separated from the reaction mixture and the ionic liquid was reused four times after removing water. No significant loss in catalytic activity was observed on recycling. (c) 2005 Elsevier B.V. All rights reserved.

10aacids10aalcohol and reaction medium10aFischer esterification10aIonic liquid1 aJoseph, Trissa1 aSahoo, S.1 aHalligudi, Shivappa, B. uhttps://library.ncl.res.in/content/bronsted-acidic-ionic-liquids-green-efficient-and-reusable-catalyst-system-and-reaction-101077nas a2200241 4500008004100000022001400041245008000055210006900135260010600204300001400310490000700324520018500331653002300516653002500539653002600564653002100590100001900611700002600630700002600656700001500682700002600697856011200723 2006 eng d a0040-403900aBeta-lactam-azasugar hybrid as a competitive potent galactosidase inhibitor0 aBetalactamazasugar hybrid as a competitive potent galactosidase  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcNOV a7923-79260 v473 a

A beta-lactam-azasugar hybrid (polyhydroxylated carbacephem) has been designed and synthesized as a potent glycosidase inhibitor. (c) 2006 Elsevier Ltd. All rights reserved.

10abeta-Galactosidase10abeta-lactam-azasugar10aglycosidase inhibitor10ahybrid molecules1 aPandey, Ganesh1 aDumbre, Shrinivas, G.1 aKhan, Mohammad, Islam1 aShabab, M.1 aPuranik, Vedavati, G. uhttps://library.ncl.res.in/content/beta-lactam-azasugar-hybrid-competitive-potent-galactosidase-inhibitor-002114nas a2200157 4500008004100000022001400041245010800055210006900163260009600232300001400328490000700342520142700349100002501776700002601801856012901827 2006 eng d a0031-865500aBinding of T-antigen disaccharides to artocarpus hirsuta lectin and jacalin are energetically different0 aBinding of Tantigen disaccharides to artocarpus hirsuta lectin a aBIOTECH PARK, 1021 15TH ST, SUITE 9, AUGUSTA, GA 30901-3158 USAbAMER SOC PHOTOBIOLOGYcSEP a1315-13180 v823 a

The thermodynamics of binding of Me-alpha-GalNAc, Gal-beta-1-3GalNAc-alpha-O-Me (T-antigen-alpha), Gal-beta-1-3GalNAc and Gal-alpha-1-6Glc (mellibiose) to Artocarpus hirsuta lectin was studied using fluorescence spectroscopy. The binding affinities of the saccharides are in the order Gal-beta-1-3GalNAc-alpha-O-Me > Me-alpha-GalNAc > Me-alpha-Gal > Gal-beta-1-3GalNAc > Gal-alpha-1-6Glc. The binding affinities were comparable to those for jacalin. However, binding of the saccharides to the A. hirsuta lectin was not affected as strongly by temperature as observed in jacalin and the trend was found to be reversed. Values for Delta H and Delta S were found to be positive in A. hirsuta lectin-disaccharide binding despite similar binding affinities. Thus, with 99% structural and 96% sequence homology, with similar sugar specificity and affinity, the energetics of the disaccharide binding of the two lectins seem to be different. Me-alpha-GalNAc binding to A. hirsuta lectin is enthalpically driven, because the association constant decreases with increasing temperature. However, the binding of the T-antigen disaccharides and mellibiose disaccharides to the lectin is entropically driven. The difference in the molecular associations in the packing and variation of the C-terminal length of the P chain of the A. hirsuta lectin could be reflected in the different disaccharide binding energetics.

1 aGaikwad, Sushama, M.1 aKhan, Mohammad, Islam uhttps://library.ncl.res.in/content/binding-t-antigen-disaccharides-artocarpus-hirsuta-lectin-and-jacalin-are-energetically-000442nas a2200121 4500008004100000022001400041245003800055210003700093260007500130300001400205100002900219856007200248 2006 eng d a0936-521400aBINOL: a versatile chiral reagent0 aBINOL a versatile chiral reagent aRUDIGERSTR 14, D-70469 STUTTGART, GERMANYbGEORG THIEME VERLAG KGcDEC a3366-33671 aPandey, Satyendra, Kumar uhttps://library.ncl.res.in/content/binol-versatile-chiral-reagent-002417nas a2200217 4500008004100000022001400041245016500055210006900220260007300289300001400362490000900376520153500385653003201920653002701952653001901979653001101998653003402009100001602043700001402059856012602073 2006 eng d a0304-416500aBiochemical characterization of a low molecular weight aspartic protease inhibitor from thermo-tolerant bacillus licheniformis: kinetic interactions with pepsin0 aBiochemical characterization of a low molecular weight aspartic  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcDEC a1845-18560 v17603 a

The present article reports a low molecular weight aspartic protease inhibitor, API, from a newly isolated thermo-tolerant Bacillus licheniformis. The inhibitor was purified to homogeneity as shown by rp-HPLC and SDS-PAGE. API is found to be stable over a broad pH range of 2-11 and at temperature 90 degrees C for 2 1/2 h. It has a Mr (relative molecular mass) of 1363 Da as shown by MALDI-TOF spectra and 1358 Da as analyzed by SDS-PAGE The amino acid analysis of the peptide shows the presence of 12 amino acid residues having Mr of 1425Da. The secondary structure of API as analyzed by the CD spectra showed 7% alpha-helix, 49% beta-sheet and 44% aperiodic structure. The Kinetic studies of Pepsin-API interactions reveal that API is a slow-tight binding competitive inhibitor with the IC(50) and K(i) values 4.0nM and (3.83 nM - 5.31 nM) respectively. The overall inhibition constant K(i)* value is 0.107 +/- 0.015 nM. The progress curves are time-dependent and consistent with slow-tight binding inhibition: E+I reversible arrow (k(4), k(5)) EI reversible arrow (k(6), k(7)) EI*. Rate constant k(6) = 2.73 +/- 0.32 s(-1) reveals a fast isomerization of enzyme-inhibitor complex and very slow dissociation as proved by k(7)=0.068 +/- 0.009s(-1). The Rate constants from the intrinsic tryptophanyl fluorescence data is in agreement with those obtained from the kinetic analysis; therefore, the induced conformational changes were correlated to the isomerization of EI to EI*. (c) 2006 Elsevier B.V. Ail rights reserved.

10aAspartic protease inhibitor10aBacillus licheniformis10aenzyme kinetic10aPepsin10aSlow-tight binding inhibition1 aKumar, Ajit1 aRao, Mala uhttps://library.ncl.res.in/content/biochemical-characterization-low-molecular-weight-aspartic-protease-inhibitor-thermo-102185nas a2200241 4500008004100000022001400041245011100055210006900166260009900235300001400334490000700348520126300355653001901618653001901637653003401656653002401690653001601714100002601730700001801756700001901774700001801793856013201811 2006 eng d a0141-391000aBiodegradation of gamma-sterilised biomedical polyolefins under composting and fungal culture environments0 aBiodegradation of gammasterilised biomedical polyolefins under c aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcMAY a1105-11160 v913 a

Polyolefin-based commodities are widely used as biomedical devices and food packaging after gamma-sterilisation. The aim of the present work was to study the effect of gamma-sterilisation on the biodegradation of polyolefins. Films of isotactic polypropylene, high-density polyethylene and ethylene-propylene (EP) copolymers were sterilised under gamma-radiation with doses of 10 and 25 kGy. Neat and sterilised samples were incubated in compost and fungal culture environments. The changes in functional groups, surface morphology and chain scission in polymer chains were characterized by FTIR spectroscopy, SEM and viscometric measurements, respectively. A gradual decrease in intrinsic viscosity [eta] and increase in carbonyl and hydroxyl regions in FTIR spectra were found for the gamma-sterilised samples as a function of increasing dose. Polypropylene was found to be more susceptible to both radio-oxidation and biodegradation. It was observed that in case of ethylene-propylene copolymers, extent of gamma-sterilisation and/or biodegradation depends on the composition and distribution of comonomers. Important surface erosion was detected by SEM, for higher sterilisation doses, after composting. (c) 2005 Elsevier Ltd. All rights reserved.

10aBiodegradation10achain scission10aComposting and fungal culture10agamma-sterilisation10aPolyolefins1 aAlariqi, Sameh, A. S.1 aKumar, A., P.1 aRao, B., S. M.1 aSingh, R., P. uhttps://library.ncl.res.in/content/biodegradation-gamma-sterilised-biomedical-polyolefins-under-composting-and-fungal-culture-101983nas a2200145 4500008004100000245015500041210006900196260000800265300001400273520135300287100002001640700002001660700003101680856012601711 2006 eng d00aBis(2-diphenylphosphinoxynaphthalen-1-yl)methane: transition metal chemistry, Suzuki cross-coupling reactions and homogeneous hydrogenation of olefins0 aBis2diphenylphosphinoxynaphthalen1ylmethane transition metal che cNOV a1322-13303 a

Transition metal complexes of bis(2-diphenylphosphinoxynaphthalen-1-yl)methane (1) are described. Bis(phosphinite) 1 reacts with Group 6 metal carbonyls, [Rh(CO)2Cl]2, anhydrous NiCl2, [Pd(C3H5)Cl]2/AgBF4 and Pt(COD)I2 to give the corresponding 10-membered chelate complexes 2, 3 and 5–8. Reaction of 1 with [Rh(COD)Cl]2 in the presence of AgBF4 affords a cationic complex, [Rh(COD){Ph2P(–OC10H6)(µ-CH2)(C10H6O–)PPh2-κP,κP}]BF4 (4). Treatment of 1 with AuCl(SMe2) gives mononuclear chelate complex, [(AuCl){Ph2P(–OC10H6)(µ-CH2)(C10H6O–)PPh2-κP,κP}] (9) as well as a binuclear complex, [Au(Cl){µ-Ph2P(–OC10H6)(µ-CH2)(C10H6O–)PPh2-κP,κP}AuCl] (10) with ligand 1 exhibiting both chelating and bridged bidentate modes of coordination respectively. The molecular structures of 2, 6, 7, 9 and 10 are determined by X-ray studies. The mixture of Pd(OAc)2 and 1 effectively catalyzes Suzuki cross-coupling reactions of a range of aryl halides with aryl boronic acid in MeOH at room temperature or at 60 °C, giving generally high yields even under low catalytic loads. The cationic rhodium(I) complex, [Rh(COD){Ph2P(–OC10H6)(µ-CH2)(C10H6O–)PPh2-κP,κP}]BF4 (4) catalyzes the hydrogenation of styrenes to afford the corresponding alkyl benzenes in THF at room temperature or at 70 °C with excellent turnover frequencies.

1 aPunji, Benudhar1 aMague, Joel, T.1 aBalakrishna, Maravanji, S. uhttps://library.ncl.res.in/content/bis2-diphenylphosphinoxynaphthalen-1-ylmethane-transition-metal-chemistry-suzuki-cross00528nas a2200145 4500008004100000245007800041210006900119260000800188300001200196490000700208100002300215700002200238700001800260856010400278 2006 eng d00aBrewster angle microscope - an excellent tool for nanoscience researchers0 aBrewster angle microscope an excellent tool for nanoscience rese cAUG a227-2290 v361 aJoshi, Bhagyashree1 aKhatavkar, Rupali1 aIyer, Neelima uhttps://library.ncl.res.in/content/brewster-angle-microscope-excellent-tool-nanoscience-researchers01831nas a2200229 4500008004100000022001400041245006500055210006500120260009500185300001400280490000600294520104900300653001701349653001701366653002301383100002101406700002101427700001401448700002101462700001901483856009901502 2007 eng d a1533-488000aBacterial enzyme mediated biosynthesis of gold nanoparticles0 aBacterial enzyme mediated biosynthesis of gold nanoparticles a25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USAbAMER SCIENTIFIC PUBLISHERScDEC a4369-43770 v73 a

Development of synthesis methods for anisotropic metal nanoparticles is of considerable interest due to their remarkable optoelectronic properties. Various shapes ranging from rods to cubes to tetrapods and prisms may be obtained by chemical methods. Here we show that anisotropic gold nanoparticles can be synthesized biologically by the bacterium Actinobacter spp. when challenged with gold chloride in the presence of Bovine serum albumin (BSA). We also observed that synthesis of gold nanoparticles occur with simultaneous induction of the protease enzyme secreted by the bacterium in the presence of BSA. The presence of BSA helps to enhance the rate of gold nanoparticles biosynthesis and may also impart some shape control. Controlling simple experimental conditions like incubation temperature and presence or absence of oxygen have drastic effect on the reaction rate and the morphology of the particles. Various assay experiments show that the presence of enzyme protease can act as a reducing as well as shape directing agent.

10aBiosynthesis10abovine serum10agold nanoparticles1 aBharde, Atul, A.1 aKulkarni, Aarohi1 aRao, Mala1 aPrabhune, Asmita1 aSastry, Murali uhttps://library.ncl.res.in/content/bacterial-enzyme-mediated-biosynthesis-gold-nanoparticles-001919nas a2200205 4500008004100000022001400041245018300055210006900238260007300307300001200380490000600392520104200398653002401440653004001464653002501504100001901529700002001548700001801568856012701586 2007 eng d a1566-736700aBenzylsulfonic acid functionalized mesoporous Zr-TMS catalysts: an efficient and recyclable catalyst for the preparation of coumarin derivatives by pechmann condensation reaction0 aBenzylsulfonic acid functionalized mesoporous ZrTMS catalysts an aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAY a777-7830 v83 a

Benzylsulfonic acid functionalized mesoporous Zr-TMS (Zr-TMS-BSA, Zr-TMS, zirconia based transition metal oxide mesoporous molecular sieves) catalyst is used as an alternative to conventional acid catalysts in the von Pechmann condensation of aromatic alcohols with ethyl acetoacetate leading to the formation of coumarin derivatives in solvent free condition at 150 degrees C. Three types of coumarins, 7-hydroxy-4-methylcoumarin (beta-methylumbelliferone), 7-methoxy-4-methylcoumarin (Methyl-4-methylumbelliferyl ether) and 7,8-benzo-4-methylcoumarin derivatives have been prepared using resorcinol, 3-methoxyphenol and 1-naphthol, respectively. The catalytic activity and the recycle studies of the catalyst in all the three reactions proved the superior performance of the catalyst. Further the products were confirmed by GC, elemental analysis, melting point and NMR techniques. Finally, the probable mechanism for Pechmarm condensation using Zr-TMS-BSA catalyst has been reported. (c) 2006 Elsevier B.V. All rights reserved.

10abenzylsulfonic acid10aPechmann condensation and coumarins10aZr-TMS-BSA catalysts1 aSelvakumar, S.1 aChidambaram, M.1 aSingh, A., P. uhttps://library.ncl.res.in/content/benzylsulfonic-acid-functionalized-mesoporous-zr-tms-catalysts-efficient-and-recyclable02107nas a2200241 4500008004100000022001400041245014100055210006900196260008600265300001200351490000700363520118300370653001601553653002401569653001901593653001401612100001801626700001901644700002501663700002001688700002501708856013201733 2007 eng d a1521-654300aBile salt hydrolase, the member of Ntn-hydrolase family: differential modes of structural and functional transitions during denaturation0 aBile salt hydrolase the member of Ntnhydrolase family differenti a325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USAbTAYLOR & FRANCIS INCcFEB a118-1250 v593 a

Conformational transitions and functional stability of the bile salt hydrolase (BSH; cholylglycine EC: 3.5.1.24) from Bifidobacterium longum (BlBSH) cloned and expressed in E. coli were studied under thermal, chemical and pH-mediated denaturation conditions using fluorescence and CD spectroscopy. Thermal and Gdn-HCl-mediated denaturation of BlBSH is a multistep process of inactivation and unfolding. The inactivation and unfolding of the enzyme was found to be irreversible. Enzyme activity seems sensitive to even minor conformational changes at the active site. Thermal denaturation as such did not result in any insoluble protein aggregates. However, on treating with 0.25-1 M Gdn-HCl the enzyme showed increasing aggregation at temperatures of 40-55 degrees C indicating more complex structural changes taking place in the presence of chemical denaturants. The enzyme secondary structure was still intact at acidic pH (pH 1-3). The perturbation in the tertiary structure at the acidic pH was detected through freshly formed solvent exposed hydrophobic patches on the enzyme. These changes could be due to the formation of an acid-induced molten globule-like state.

10aAggregation10aBile salt hydrolase10amolten-globule10aUnfolding1 aKumar, Suresh1 aSuresh, C., G.1 aBrannigan, James, A.1 aDodson, Guy, G.1 aGaikwad, Sushama, M. uhttps://library.ncl.res.in/content/bile-salt-hydrolase-member-ntn-hydrolase-family-differential-modes-structural-and-functional01672nas a2200229 4500008004100000022001400041245013600055210006900191260007300260300001200333490000600345520074600351653003001097653003601127653005001163653001701213653001701230100001901247700001701266700002901283856013001312 2007 eng d a1566-736700aBimetallic Pt-Sn/gamma-alumina catalyst for highly selective liquid phase hydrogenation of diethyl succinate to gamma-butyrolactone0 aBimetallic PtSngammaalumina catalyst for highly selective liquid aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR a340-3440 v83 a

Platinum-tin bimetallic catalyst on gamma-alumina support was prepared by impregnation method and was reduced by sodium borohydride at room temperature. XRD and XPS characterization revealed that platinum was reduced to Pt-0 while, tin was probably partially reduced to Sn2+ due to the low temperature reduction method and Sn-0 was completely absent, avoiding the formation of P-Sn alloy. Pt-Sn/gamma-alumina (Pt 1%, Sn 9%) thus prepared was found to give almost complete selectivity to gamma-butyrolactone in liquid phase hydrogenation of diethyl succinate. A plausible reaction pathway is proposed involving Pt-O-Sn state and high selectivity to GBL is due to the Lewis acidity of Sn2+/4+ (c) 2006 Elsevier B.V. All rights reserved.

10abimetallic Pt-Sn catalyst10agamma-butyrolactone selectivity10aliquid phase hydrogenation of diethyl maleate10aXPS of Pt-Sn10aXRD of Pt-Sn1 aVaidya, S., H.1 aRode, C., V.1 aChaudhari, Raghunath, V. uhttps://library.ncl.res.in/content/bimetallic-pt-sngamma-alumina-catalyst-highly-selective-liquid-phase-hydrogenation-diethyl02040nas a2200241 4500008004100000022001400041245015900055210006900214260010300283300001400386490000700400520107700407653003401484653001301518653001701531653002101548653002301569653002401592653001301616100002401629700001401653856013101667 2007 eng d a0300-908400aBiochemical characterization of a novel beta-1-3, 1-4 glucan 4-glucanohydrolase from thermomonospora sp having a single active site for lichenan and xylan0 aBiochemical characterization of a novel beta13 14 glucan 4glucan a23 RUE LINOIS, 75724 PARIS, FRANCEbELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIERcDEC a1489-14970 v893 a

A bifunctional high molecular weight (Mr, 64,500 Da) beta-1-3, 1-4 glucan 4-glucanohydrolase was purified to homogeneity from Thermomonospora sp., exhibiting activity towards lichenan and xylan. A kinetic method was used to analyze the active site that hydrolyzes lichenan and xylan. The experimental data was in agreement with the theoretical values calculated for a single active site. Probing the conformation and microenvironment at active site of the enzyme by fluorescent chemo-affinity label, OPTA resulted in the formation of an isoindole derivative with complete inactivation of the enzyme to hydrolyse both lichenan and xylan confirmed the results of kinetic method. OPTA forms an isoindole derivative by cross-linking the proximal thiol and amino groups. The modification of cysteine and lysine residues by DTNB and TNBS respectively abolished the ability of the enzyme to form an isoindole derivative with OPTA, indicating the participation of cysteine and lysine in the formation of isoindole complex. (C) 2007 Elsevier Masson SAS. All rights reserved.

10a1-4 glucan 4-glucanohydrolase10abeta-1-310abifunctional10aO-phthaialdehyde10asingle active site10aThermomonospora sp.10aXylanase1 aAnish, Ramakrishnan1 aRao, Mala uhttps://library.ncl.res.in/content/biochemical-characterization-novel-beta-1-3-1-4-glucan-4-glucanohydrolase-thermomonospora-002197nas a2200217 4500008004100000022001400041245017500055210006900230260007900299300001200378490000900390520129600399653001501695653002401710653001901734653003601753653003201789100002101821700001401842856012301856 2007 eng d a0096-300300aBiochemical characterization of an aspartic protease from vigna radiata: kinetic interactions with the classical inhibitor pepstatin implicating a tight binding mechanism0 aBiochemical characterization of an aspartic protease from vigna  a360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAbELSEVIER SCIENCE INCcMAY a619-6270 v17743 a

{Aspartic proteases are the focus of recent research interest in understanding the physiological importance of this class of enzymes in plants. This is the first report of an aspartic protease from the seeds of Vigna radiata. The aspartic protease was purified to homogeneity by fractional ammonium sulfate precipitation and pepstatin-A agarose affinity column. It was found to have a molecular weight of 67,406 Da by gel filtration chromatography. SDS-PAGE analysis revealed the presence of a heterodimer with subunits of molecular weights of 44,024 and 23,349 Da respectively. The enzyme was pH stable with the amino acid analysis confirming the molecular weight of the protein. The substrate cleavage site as analyzed by using the synthetic substrate was found to be the Phe-Tyr bond. The kinetic interactions of the enzyme were studied with the universal inhibitor, pepstatin A. This is the first report on the interactions of a plant aspartic protease with pepstatin-A, an inhibitor from a microbial source. A competitive one-step mechanism of binding is observed. The progress curves are time-dependent and consistent with tight binding inhibition. The K(i) value of the reversible complex of pepstatin with the enzyme was 0.87 mu M whereas the overall inhibition constant K(i)*

10aant colony10aglobal optimization10ametaheuristics10amultimodal continuous functions10aparticle swarm optimization1 aKulkarni, Aarohi1 aRao, Mala uhttps://library.ncl.res.in/content/biochemical-characterization-aspartic-protease-vigna-radiata-kinetic-interactions-000448nas a2200133 4500008004100000245004100041210004100082260004700123300001000170100001700180700002700197700001700224856007300241 2007 eng d00aBioconjugation of soft nanomaterials0 aBioconjugation of soft nanomaterials aHoboken, NJ, USA.bJohn Wiley & Sons, Inc. a61-911 aSingh, Neetu1 aBlackburn, William, H.1 aLyon, Andrew uhttps://library.ncl.res.in/content/bioconjugation-soft-nanomaterials01792nas a2200217 4500008004100000022001400041245009300055210006900148260011000217300001400327490000700341520097000348100002401318700001701342700001701359700001701376700002601393700001901419700001701438856011901455 2007 eng d a0959-942800aBio-milling technique for the size reduction of chemically synthesized BiMnO3 nanoplates0 aBiomilling technique for the size reduction of chemically synthe aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAUG a3910-39140 v173 a

Wet- chemical techniques for the synthesis of complex oxide materials have advanced significantly; however, achieving finely dispersed nanoparticles with sizes less than 10 nm still remains challenging, especially for the perovskite family of compounds. On the other hand, a fungusmediated synthesis technique has recently shown potential to synthesize perovskites such as BaTiO3 with sizes as small as 5 nm. Here we report, for the first time, the use of fungal biomass, at room temperature, to break down chemically synthesized BiMnO3 nanoplates ( size similar to 150 - 200 nm) into very small particles (, 10 nm) while maintaining their crystalline structure and the phase purity. This novel technique that we have named as `` bio- milling'' holds immense potential for synergically utilizing both chemical and biological synthesis techniques to synthesize complex oxide nanoparticles with particle sizes less than 10 nm with the proper crystalline phase.

1 aMazumder, Baishakhi1 aUddin, Imran1 aKhan, Shadab1 aRavi, Venkat1 aSelvraj, Kaliaperumal1 aPoddar, Pankaj1 aAhmad, Absar uhttps://library.ncl.res.in/content/bio-milling-technique-size-reduction-chemically-synthesized-bimno3-nanoplates-002341nas a2200169 4500008004100000022001400041245007300055210006800128260007100196300001400267490000700281520170300288100002301991700002502014700002902039856010302068 2007 eng d a0888-588500aBiphasic hydroformylation of 1,4-diacetoxy-2-butene: a kinetic study0 aBiphasic hydroformylation of 14diacetoxy2butene a kinetic study a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcDEC a8629-86370 v463 a

Hydroformylation of 1,4-diacetoxy-2-butene was studied using a water-soluble Rh complex catalyst prepared in situ from [Rh(COD)Cl](2) complex and trisodium salt of tri-(m-sulfophenyl)phosphine (TPPTS) in a biphasic system. The sequence of addition of catalyst precursor, ligand, and reactant/solvent showed a significant effect on leaching of Rh from aqueous to organic phase, and hence, the procedure was optimized to develop a nonleaching and stable biphasic catalyst system. The only hydroformylation product (1,4-diacetoxy-2-formyl butane, DAFB) formed was found to deacetoxylate completely to 2-formyl-4-acetoxybutene (FAB), thus allowing a one-pot synthesis of FAB, an important intermediate for Vitamin A. Experimental data on the concentration-time and CO/H-2 consumption-time profiles were obtained, and the effects of DAB concentration, CO partial pressure, H-2 partial pressure, and catalyst concentration were studied in a stirred batch reactor over a temperature range of 338-358 K. The effect of aqueous phase holdup on the initial rate of hydroformylation and analysis of gas-liquid and liquid-liquid mass transfer effects were also investigated to identify the reaction rate data operating in a kinetic regime. A rate equation based on the known hydroformylation reaction mechanism was used to fit the experimental rate data and to evaluate kinetic parameters. The agreement between the model prediction and the experimental data was found to be excellent. The activation energy was calculated as 30.1 kJ/mol. The biphasic catalyst system reported here is not only efficient for catalyst-product separation but also provides a tandem synthesis of Vitamin A intermediate, FAB.

1 aChansarkar, Rashmi1 aKelkar, Ashutosh, A.1 aChaudhari, Raghunath, V. uhttps://library.ncl.res.in/content/biphasic-hydroformylation-14-diacetoxy-2-butene-kinetic-study-001538nas a2200229 4500008004100000022001400041245007300055210006900128260007300197300001200270490000800282520072400290653001501014653002401029653002801053653003901081100002301120700002401143700001801167700001901185856010401204 2007 eng d a0039-602800aBlocking effects in irreversible adsorption of linear macromolecules0 aBlocking effects in irreversible adsorption of linear macromolec aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN a274-2790 v6013 a

{A new variant of random sequential adsorption (RSA), namely random sequential ballistic adsorption (RSBA), is proposed to explore the possible role of blocking effects in the adsorption dynamics of ballistically arriving objects. These objects upon adsorption can protrude outside the substrate and in turn can obstruct and hence reject the adsorption of newly arriving objects. Adsorption of linear macromolecules (modeled as infinitesimally thin needles), on a two-dimensional continuum substrate is studied using RSBA model. It is shown analytically that in late time regime, the number n(t) of adsorbed objects at time t follows a power law n(t) similar to t(alpha), as in RSA, but with a different exponent

10aAdsorption10aadsorption kinetics10aMonte Carlo simulations10arandom sequential adsorption (RSA)1 aShelke, Pradip, B.1 aBanpurkar, Arun, G.1 aOgale, S., B.1 aLimaye, A., V. uhttps://library.ncl.res.in/content/blocking-effects-irreversible-adsorption-linear-macromolecules-002062nas a2200205 4500008004100000022001400041245011100055210006900166260007300235300001400308490000900322520124800331653003501579653002901614653003201643653002201675100001601697700001901713856012401732 2007 eng d a1570-963900aBowman-birk protease inhibitor from the seeds of vigna unguiculata forms a highly stable dimeric structure0 aBowmanbirk protease inhibitor from the seeds of vigna unguiculat aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT a1264-12730 v17743 a

Different protease inhibitors including Bowman-Birk type (BBI) have been reported from the seeds of rigna unguiculata. Protease isoinhibitors of double-headed Bowman-Birk type from the seeds of Vigna unguiculata have been purified and characterized. The BBI from Vigna unguiculata (Vu-BBI) has been found to undergo self-association to form very stable dimers and more complex oligomers, by size-exclusion chromatography and SDS-PAGE in the presence of urea. Many BBIs have been reported to undergo self-association to form homodimers or more complex oligomers in solution. Only one dimeric crystal structure of a BBI (pea-BBI) is reported to date. We report the three-dimensional structure of a Vu-BBI determined at 2.5 A resolution. Although, the inhibitor has a monomer fold similar to that found in other known structures of Bowman-Birk protease inhibitors, its quaternary structure is different from that commonly observed in this family. The structural elements responsible for the stability of monomer molecule and dimeric association are discussed. The Vu-BBI may use dimeric or higher quaternary association to maintain the physiological state and to execute its biological function. (C) 2007 Elsevier B.V. All rights reserved.

10aBowman-Birk protease inhibitor10aplant protease inhibitor10aprotein-protein interaction10aVigna unguiculata1 aRao, K., N.1 aSuresh, C., G. uhttps://library.ncl.res.in/content/bowman-birk-protease-inhibitor-seeds-vigna-unguiculata-forms-highly-stable-dimeric-002067nas a2200229 4500008004100000022001400041245010500055210006900160260007300229300001200302490000800314520122600322653002101548653001201569653001501581653002401596653001201620100001901632700002301651700003301674856013001707 2007 eng d a1381-116900aButylation of toluene: influence of zeolite structure and acidity on 4-tert-butyltoluene selectivity0 aButylation of toluene influence of zeolite structure and acidity aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR a109-1160 v2653 a

Vapour phase alkylation of toluene with tert-butyl alcohol was studied over the large pore zeolites H beta, HY and HMCM-22. The influence of the acidity, reaction temperature, run duration, feed rate (WHSV) and molar ratio of the reactants on conversion of toluene and selectivity for 4-tertbutyl toluene was studied. Among the isomers formed during the reaction, the para-isomer was the predominant one, followed by the meta-isomer, while no ortho-isomer was found in the products due to steric factors. 4-tert-Butyl toluene selectivity decreases at higher temperatures, while going through a maximum in the 413-433 K temperature range. Zeolite HY with a high silica to alumina ratio (SiO2/Al2O3 = 80), that has strong acidity, but lower acid site density, possesses high activity for toluene conversion, high alkylation selectivity and also high para-selectivity. The improved para-selectivity of high silica HY zeolite is attributed to the low isomerization activity that results in the suppression of the secondary isomerization of 4-tert-butyl toluene. Both alkylation and selectivity are higher with feed mixtures containing a lower fraction of tert-butyl alcohol. (c) 2006 Elsevier B.V. All rights reserved.

10a4-t-butyltoluene10aAcidity10abutylation10alarge pore zeolites10aToluene1 aPai, Shivanand1 aGupta, Upendranath1 aChilukuri, Satyanarayana, V. uhttps://library.ncl.res.in/content/butylation-toluene-influence-zeolite-structure-and-acidity-4-tert-butyltoluene-selectivity01757nas a2200277 4500008004100000022001400041245006100055210006000116260009200176300001400268490000600282520086800288653002401156653001801180653000801198653000801206653000801214100002201222700001801244700002301262700002301285700002301308700003001331700002401361856009401385 2008 eng d a1533-488000aBacterial synthesis of copper/copper oxide nanoparticles0 aBacterial synthesis of coppercopper oxide nanoparticles a26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USAbAMER SCIENTIFIC PUBLISHERScJUN a3191-31960 v83 a

A bacterial mediated synthesis of copper/copper oxide nanoparticle composite is reported. A Gram-negative bacterium belonging to the genus Serratia was isolated from the midgut of Stibara sp., an insect of the Cerambycidae family of beetles found in the Northwestern Ghats of India. This is a unique bacterium that is quite specific for the synthesis of copper oxide nanoparticles as several other strains isolated from the same insect and common Indian mosquitoes did not result in nanoparticle formation. By following the reaction systematically, we could delineate that the nanoparticle formation occurs intracellularly. However, the process results in the killing of bacterial cells. Subsequently the nanoparticles leak out as the cell wall disintegrates. The nanoparticles formed are thoroughly characterized by UV-Vis, TEM, XRD, XPS and FTIR studies.

10abacterial synthesis10aNanoparticles10aTEM10aXPS10aXRD1 aHasan, Syed, Saif1 aSingh, Sanjay1 aParikh, Rasesh, Y.1 aDharne, Mahesh, S.1 aPatole, Milind, S.1 aPrasad, Bhagavatula L. V.1 aShouche, Yogesh, S. uhttps://library.ncl.res.in/content/bacterial-synthesis-coppercopper-oxide-nanoparticles-001714nas a2200205 4500008004100000022001400041245005700055210005600112260011000168300001400278490000700292520099100299100001801290700002501308700001901333700001701352700001901369700003001388856009001418 2008 eng d a0959-942800aBacterial synthesis of silicon/silica nanocomposites0 aBacterial synthesis of siliconsilica nanocomposites aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAPR a2601-26060 v183 a

The synthesis of silicon/silica nanoparticle composites by the bacterium Actinobacter sp. is demonstrated. More specifically, the formation of silicon/silica nanocomposite is shown to occur when the bacterium is exposed to K(2)SiF(6) precursor under ambient conditions. Based on the earlier reports where this bacterium has been shown to synthesize iron oxide and iron sulfide nanoparticles, it is hypothesized that this bacterium secretes reductases and oxidising enzymes, which lead to the Si/SiO(2) nanocomposite synthesis. The particles obtained by bacterial synthesis were thoroughly characterized. The cytotoxicity studies revealed that the particles do not display any cytotoxicity to human skin cells. The synthesis of silica nanoparticles by bacteria in the present study demonstrates the versatility of the organism, and the formation of elemental silicon by this environmentally friendly process expands further the scope of microorganism based nanomaterial synthesis.

1 aSingh, Sanjay1 aBhatta, Umananda, M.1 aSatyam, P., V.1 aDhawan, Alok1 aSastry, Murali1 aPrasad, Bhagavatula L. V. uhttps://library.ncl.res.in/content/bacterial-synthesis-siliconsilica-nanocomposites-002037nas a2200253 4500008004100000022001400041245011800055210006900173260007100242300001400313490000700327520109000334100002101424700002301445700002201468700001801490700002301508700002001531700003001551700002401581700002901605700001901634856013001653 2008 eng d a0743-746300aBacteria-mediated precursor-dependent biosynthesis of superparamagnetic iron oxide and iron sulfide nanoparticles0 aBacteriamediated precursordependent biosynthesis of superparamag a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcJUN a5787-57940 v243 a

The bacterium Actinobacter sp. has been shown to be capable of extracellularly synthesizing iron based magnetic nanoparticles, namely maghemite (gamma-Fe2O3) and greigite (Fe3S4) Under ambient conditions depending on the nature of precursors used. More precisely, the bacterium synthesized maghemite when reacted with ferric chloride and iron sulfide when exposed to the aqueous solution of ferric chloride-ferrous sulfate. Challenging the bacterium with different metal ions resulted in induction of different proteins, which bring about the specific biochemical transformations in each case leading to the observed products. Maghemite and iron sulfide nanoparticles show superparamagnetic characteristics as expected. Compared to the earlier reports of magnetite and greigite synthesis by magnetotactic bacteria and iron reducing bacteria, which take place strictly under anaerobic conditions, the present procedure offers significant advancement since the reaction occurs under aerobic condition. Moreover, reaction end products can be tuned by the choice of precursors used.

1 aBharde, Atul, A.1 aParikh, Rasesh, Y.1 aBaidakova, Marina1 aJouen, Samuel1 aHannoyer, Beatrice1 aEnoki, Toshiaki1 aPrasad, Bhagavatula L. V.1 aShouche, Yogesh, S.1 aOgale, Satishchandra, B.1 aSastry, Murali uhttps://library.ncl.res.in/content/bacteria-mediated-precursor-dependent-biosynthesis-superparamagnetic-iron-oxide-and-iron-002578nas a2200229 4500008004100000022001400041245019000055210006900245260007300314300001000387490000700397520165500404653002102059653002902080653002002109653002102129653001702150100001702167700001802184700001902202856012702221 2008 eng d a0924-857900aBactericidal activity of 2-nitroimidazole against the active replicating stage of mycobacterium bovis BCG and mycobacterium tuberculosis with intracellular efficacy in THP-1 macrophages0 aBactericidal activity of 2nitroimidazole against the active repl aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUL a40-450 v323 a

This study evaluated the antituberculous potential of 2-nitroimidazole under in vitro conditions. Minimal bactericidal concentrations of the compound against actively replicating Mycobacterium bovis BCG and Mycobacterium tuberculosis H37Ra were found to be 0.226 mu g/mL and 0.556 mu g/mL in enriched and minimal medium, respectively. Minimal inhibitory concentrations were > 100 times lower than reported antituberculous nitroimidazoles such as nitrofurantoin and furaltadone, indicating the greater potential of 2-nitroimidazole. No discernible effect of 2-nitroimidazole was seen on saprophytic Mycobacterium smegmatis and the representative bacterial strain Escherichia coli DH5 alpha, indicating the specificity of the molecule against tuberculous mycobacteria. The compound was also found to be effective against M. tuberculosis in the intracellular environment of the human monocytic cell line THP-1, with a reduction in viability of bacilli by 2.5 log after 144 h of incubation at a concentration of 0.113 mu g/mL. A five-fold higher concentration (0.565 mu g/mL) of 2-nitroimidazole sterilised the macrophages of intracellular pathogens within 192 h, without affecting the host. However, 2-nitroimidazole was unable to affect significantly the viability of dormant non-replicating bacilli of M. bovis BCG and M. tuberculosis in Wayne's in vitro model. Overall, the results indicate that 2-nitroimidazole is a potent antituberculous agent active against the organism's active replicating stage, with promising intracellular efficacy as well. (c) 2008 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

10a2-nitroimidazole10aantimycobacterial agents10anitroimidazoles10aTHP-1 macrophage10atuberculosis1 aKhan, Arshad1 aSarkar, Sampa1 aSarkar, Dhiman uhttps://library.ncl.res.in/content/bactericidal-activity-2-nitroimidazole-against-active-replicating-stage-mycobacterium-001830nas a2200205 4500008004100000022001400041245010600055210006900161260009900230300001400329490000700343520102100350653001801371653001401389653003901403653001101442100003101453700002001484856012001504 2008 eng d a0960-852400aBiocomposites of cellulose reinforced starch: improvement of properties by photo-induced crosslinking0 aBiocomposites of cellulose reinforced starch improvement of prop aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcDEC a8803-88090 v993 a

In the present study, the composite films have been prepared from the aqueous dispersions of starch with microcrystalline cellulose using glycerol as plasticizer and irradiated under ultraviolet (UV) light using sodium benzoate as photo-sensitizer. Photocrosslinking was characterized by measuring the water absorption under 100% relative humidity, swelling degree and gel fraction in dimethylsulphoxide (DMSC), upon irradiation time. Both, the incorporation of cellulose and photo-irradiation were found to decrease the water absorption, swelling in DMSO and increase the gel fraction. Thermal transitions indicated the anti-plasticization of amylopectin chains at the fiber/matrix interface. With increasing content of cellulose and photo-irradiation time, the tensile modulus and strength were found to improve. It is summarized that the combination of cellulose reinforcement and photo-crosslinking of matrix has improved the physical and mechanical properties. (C) 2008 Elsevier Ltd. All rights reserved.

10aBiocomposites10acellulose10aPhoto-irradiation and crosslinking10aStarch1 aKumar, Annamalai, Pratheep1 aSingh, Raj, Pal uhttps://library.ncl.res.in/content/biocomposites-cellulose-reinforced-starch-improvement-properties-photo-induced-002025nas a2200265 4500008004100000020002200041022001400063245007900077210006900156260008300225300001200308490000900320520111100329653002901440653002701469653002601496100002101522700002401543700002301567700001101590700001701601700001801618700002101636856010201657 2008 eng d a978-0-7354-0549-3 a0094-243X00aBlob-spring model for the dynamics of ring polymer in obstacle environment0 aBlobspring model for the dynamics of ring polymer in obstacle en a2 Huntington Quadrangle, STE 1no1, Melville, NY 11747-4501 USAbSoc RheolcAUG a445-4470 v10273 a

The dynamical behavior of cyclic macromolecules in a fixed obstacle (FO) environment is very different than the behavior of linear chains in the same topological environment; while the latter relax by a snake-like reptational motion from their chain ends(1) the former can relax only by contour length fluctuations since they are endless.(2) Duke, Obukhov and Rubinstein proposed a scaling model (the DOR model) to interpret the dynamical scaling exponents shown by Monte Carlo simulations of rings in a FO environment.(2) We present a model (blob-spring model) to describe the dynamics of flexible and non-concatenated ring polymer in FO environment based on a theoretical formulation developed for the dynamics of an unentangled fractal polymer.(3) We argue that the perpetual evolution of ring perimeter by the motion of contour segments results in an extra frictional load. Our model predicts self-similar dynamics with scaling exponents for the molecular weight dependence of diffusion coefficient and relaxation times that are in agreement with the scaling model proposed by Obukhov et al.(2).

10afractal polymer dynamics10alinear viscoelasticity10aring polymer dynamics1 aLele, Ashish, K.1 aIyer, Balaji, V. S.1 aJuvekar, Vinay, A.1 aCo, A.1 aLeal, L., G.1 aColby, R., H.1 aGiacomin, A., J. uhttps://library.ncl.res.in/content/blob-spring-model-dynamics-ring-polymer-obstacle-environment-002664nas a2200241 4500008004100000022001400041245013100055210006900186260007300255300001000328490000800338520179500346653002502141653001302166653001602179653001102195653001402206100001602220700002102236700001802257700002102275856012602296 2008 eng d a1387-181100aBridging the gap between micropores and mesopores by the controlled transformation of bifunctional periodic mesoporous silicas0 aBridging the gap between micropores and mesopores by the control aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcFEB a29-400 v1083 a

Novel bifunctional periodic mesoporous silicas having high content of organic groups in the pore channels and chromium in the frame wall has been synthesized by direct co-condensation method, using organo trialkoxysilanes (chloro propyl, vinyl, methyl), chromium nitrate and tetraethyl orthosilicate, in presence of cetyltrimethyl ammonium surfactants. The obtained materials show one main peak in the XRD patterns up to a molar ratio of 1: 1 between TEOS and organosilane in the synthesis gel and N-2 adsorption-desorption analysis shows that the mesopore structure remains intact after the surfactant removal process. Among the organosilanes, chloro propyl and vinyl pendant chromium samples shows a shift in the main peak to higher 2-theta values with a corresponding decrease in pore volume and pore diameter, with the percentage of organosilane functionalization. Small-pore silicas and chromium silicas can be prepared from the large pore mesoporous MCM-41, by effectively removing the organic groups by calcination. By this method, the pore size of the material can be tailored into the super-microporous region, without changing the chain length of the surfactant used in the assembly process. The shrinkage in pore size is dependent on the nature and percentage of the organic pendant groups that the chloro propyl and vinyl pendant mesoporous materials show the maximum pore size shrinkage than the small chain length methyl units. Because of the tailorable pore size and with better textural characteristics, chromium-silica materials find applications in the field of shape selective heterogeneous catalysis, as demonstrated in the oxidation reaction of cyclohexane, than the conventional metal-containing mesoporous materials. (c) 2007 Elsevier Inc. All rights reserved.

10abifunctional silicas10achromium10aCyclohexane10aMCM-4110aOxidation1 aShylesh, S.1 aSrilakshini, Ch.1 aSingh, A., P.1 aAnderson, B., G. uhttps://library.ncl.res.in/content/bridging-gap-between-micropores-and-mesopores-controlled-transformation-bifunctional-001855nas a2200193 4500008004100000022001400041245008100055210006900136260005700205300001200262490000800274520115200282653002801434653002301462653002401485100001901509700001801528856011501546 2009 eng d a1011-372X00aBenzoylation of anisole over silicotungstic acid modified mesoporous alumina0 aBenzoylation of anisole over silicotungstic acid modified mesopo a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcMAR a363-3720 v1283 a

Mesoporous alumina (MA) molecular sieves were synthesized by using aluminum sec-butoxide as Al precursor and lauric acid as the structure directing agent. The synthesized MA was functionalized with silicotungstic acid (STA) via wet impregnation method and characterized by various physico-chemical techniques. The XRD patterns of a series of HPA functionalized MA are showing the ordered structures. N(2) sorption analysis shows type IV isotherm. NH(3)-TPD measurements revealed an increase in number of acid sites with an increase in loading of STA over MA. At the same time decrease in the acidity was observed with the increase in calcination temperature of the supported materials. Functionalization of STA were also carried out over different alumina supports such as catapol-B (CB) and alumina synthesized without surfactant (ASW) and their activities were evaluated by carrying out liquid phase Friedel-Craft acylation (FC) reaction of anisole with p-toluoyl chloride in a batch reactor at 120 degrees C. Recycling was performed in the FC reaction using 30 wt% STA-MA two times and no major deactivation of the catalyst was observed.

10aFriedel-Craft acylation10aMesoporous alumina10aSilicotungstic acid1 aSelvakumar, S.1 aSingh, A., P. uhttps://library.ncl.res.in/content/benzoylation-anisole-over-silicotungstic-acid-modified-mesoporous-alumina-001984nas a2200205 4500008004100000022001400041245011900055210006900174260009900243300001400342490000800356520113000364653002801494653002601522653002501548100002201573700003001595700002601625856012701651 2009 eng d a0960-852400aBiochemical characterization of two xylanases from yeast Pseudozyma hubeiensis producing only xylooligosaccharides0 aBiochemical characterization of two xylanases from yeast Pseudoz aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcDEC a6488-64950 v1003 a

Two distinct xylanases from Pseudozyma hubeiensis NCIM 3574 were purified to homogeneity. The molecular masses of two native xylanases were 33.3 kDa (PhX33) and 20.1 kDa (PhX20). PhX33 is predominant with alpha-helix and PhX20 contained predominantly beta-sheets. Xylanase, PhX33, possesses three tryptophan and one carboxyl residues at the active site. The active site of PhX20 comprises one residue each of tryptophan, carboxyl and histidine. Carboxyl residue is mainly involved in catalysis and tryptophane residues are solely involved in substrate binding. Histidine residue present at the active site of PhX20 appeared to have a role in substrate binding. Both the xylanases produced only xylooligosaccharides (XOS) with degree of polymerization (DP) 3-7 without formation of xylose and xylobiose. These XOS could be used in functional foods or as prebiotics. Lc ms-ms ion search of cryptic digestion of these xylanases revealed that there is no significant homology of peptides with known fungal xylanase sequences which indicate that these xylanases appear to be new. (c) 2009 Elsevier Ltd. All rights reserved.

10aCellulase-free xylanase10aPseudozyma hubeiensis10aXylooligosaccharides1 aAdsul, Mukund, G.1 aBastawade, Kulbhushan, B.1 aGokhale, Digambar, V. uhttps://library.ncl.res.in/content/biochemical-characterization-two-xylanases-yeast-pseudozyma-hubeiensis-producing-only-001794nas a2200229 4500008004100000022001400041245009000055210006900145260009900214300001200313490000700325520097900332653001901311653002301330653001501353653001101368653001201379653001401391100001801405700002001423856012101443 2009 eng d a0964-830500aBiodegradation and phenol tolerance by recycled cells of candida tropicalis NCIM 35560 aBiodegradation and phenol tolerance by recycled cells of candida aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJUN a539-5420 v633 a

Resting cells of Candida tropicalis NCIM 3556 rapidly degraded almost completely 2 g L(-1) phenol in 16h. In this study, we explored the possibility of further increasing the efficiency of the culture by repeatedly reusing the cell for biodegradation. The effect of continuous recycling of whole cells of C. tropicalis, for biodegradation of phenol indicated that though with each recycle of the cell there was steady decline in phenol biodegradation the conversion was appreciable for five recycle (similar to 70%) and reached half-life of 50% after eleven recycles. Inhibition due to substrate, recycling of cells and adaptation of residual cell were estimated and an equation derived; which indicated that the cell resilience to phenol increased with each cycle and at the end of eleven recycle adaptation was 68%. However, when the adapted cells were sub cultured and showed marginal increase <10% in biodegradation. (C) 2009 Elsevier Ltd. All rights reserved.

10aBiodegradation10aCandida tropicalis10aInhibition10aphenol10aRecycle10aTolerance1 aVarma, R., J.1 aGaikwad, B., G. uhttps://library.ncl.res.in/content/biodegradation-and-phenol-tolerance-recycled-cells-candida-tropicalis-ncim-3556-002759nas a2200229 4500008004100000022001400041245008300055210006900138260007300207300000800280490000800288520196600296653001002262653001602272653002402288653000802312653002202320100002702342700002502369700001602394856011902410 2009 eng d a0022-286000aBond length variations: electron number profiles and transferable atomic sizes0 aBond length variations electron number profiles and transferable aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcNOV a1-80 v9363 a

A profile of the number of electrons with distance along the M-X bond in gas-phase diatomic molecules has been obtained from electron density plots calculated using DFT B3LYP 6-311G** method for some representative molecules. This ``number profile'' is compared with that expected from the partitioning of the 1D bond-distance into atom-specific transferable ``hub'' or core atomic sizes of the M and X atoms and another ``axle'' size which is associated with a pair of (bonding) electrons. The ``hub'' size is proportional to a core atom-specific size, r(nZ)(c) with r(nZ)(c)(M) >= r(nZ)(c)(X). For ``single bonds'', the ``hub'' size for M atom is C(M)r(nZ)(c)(M) and for X atom is C(X)r(nZ)(c)(X). The ``axle'' size, DMX, is usually the ordinary (similar to 4a(H)/3 where a(H) is the Bohr radius of the hydrogen atom) or elongated (similar to 2a(H)) bond length of the hydrogen molecule. The ``hub'' and ``axle'' sizes could be characterized ``charge-transfer'' (C(M) = pi(2/3) = 2.144; C(X) = pi(4/3)/2 = 2.300 and D(MX) = 4a(H)/3) or ``neutral'' (CM or C(X) = 1, 2, ... and D(MX) = 2a(H)). We use a new ``static'' or ``peripatetic'' classification for the core sizes which is derived from a new condition for metallization in elements based on atomic size. The charge-transfer distance, d(MX)(+/-), is usually found for ``static'' conditions while the ``neutral'' description is usually found when X = F or for ``peripatetic'' conditions. Such a partitioning is seen to agree with that from the plot of the total number of electrons, N(el), vs r along a bond axis. The Nel vs r plots from each atom are described by a simple hydrogen-atom-like function which differ away (''out'') or towards (''in'') the M-X bond. Thus N(in,out)(M, X) = (Z(M,X) +/- 1) exp (-r/B(in,out)) where the minus sign is associated with M and plus sign with X and Bin, out being related inversely to the Slater orbital exponent. (C) 2009 Elsevier B.V. All rights reserved.

10aB3LYP10aBond length10aBond-stretch isomer10aDFT10aDiatomic molecule1 aGanguly, Parthasarathy1 aKulkarni, Bhakti, S.1 aPal, Sourav uhttps://library.ncl.res.in/content/bond-length-variations-electron-number-profiles-and-transferable-atomic-sizes-001541nas a2200229 4500008004100000022001400041245009200055210006900147260007900216300001200295490000800307520072400315653002401039653002101063653001701084653002601101653001601127653001601143100001601159700002101175856011501196 2009 eng d a0273-228900aBrevibacillus sp: a novel thermophilic source for the production of bile salt hydrolase0 aBrevibacillus sp a novel thermophilic source for the production  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcMAY a254-2620 v1573 a

A thermophilic microorganism growing within the temperature range of 40-65 A degrees C (optimum at 55 A degrees C) was isolated from hot water springs near Konkan, Maharashtra, India. Based on 16S rDNA sequence analysis, it was concluded that the isolate belongs to the genus Brevibacillus. The present paper reports the isolation, identification, and standardization of fermentation conditions for the production of enzyme, bile salt hydrolase (EC 3.5.1.24) which is produced intracellularly at high temperatures. This is the first report regarding the production of bile salt hydrolase from a thermophilic source. Optimization of fermentation conditions resulted in a 2.9-fold enhancement in enzyme production.

10aBile salt hydrolase10aBrevibacillus sp10aFermentation10aGlycodeoxycholic acid10aHot springs10aThermophile1 aSridevi, N.1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/brevibacillus-sp-novel-thermophilic-source-production-bile-salt-hydrolase-001382nas a2200169 4500008004100000020002200041022001400063245008700077210006900164260007400233300001200307490000800319520073000327100002601057700001101083856011801094 2010 eng d a978-1-4419-5978-2 a0065-259800aBacterial biosurfactants, and their role in microbial enhanced oil recovery (MEOR)0 aBacterial biosurfactants and their role in microbial enhanced oi aHeidelberger Platz 3, D-14197 Berlin, GermanybSpringer-Verlag Berlin a146-1570 v6723 a

Surfactants are chemically synthesized surface-active compounds widely used for large number of applications in various industries. During last few years there is increase demand of biological surface-active compounds or biosurfactants which are produced by large number of microorganisms as they exert biodegradability, low toxicity and widespread application compared to chemical surfactants. They can be used as emulsifiers, de-emulsifiers, wetting agents, spreading agents, foaming agents, functional food ingredients and detergents. Various experiments at laboratory scale on sand-pack columns and field trials have successfully indicated effectiveness of biosurfactants in microbial enhanced oil recovery (MEOR).

1 aKhire, Jayant, Malhar1 aSen, R uhttps://library.ncl.res.in/content/bacterial-biosurfactants-and-their-role-microbial-enhanced-oil-recovery-meor-001819nas a2200253 4500008004100000245009700041210006900138260000800207300001000215490000600225520098700231653001701218653001701235653001801252653001701270653001901287100001801306700002501324700002101349700001901370700001701389700003001406856012901436 2010 eng d00aBacterial synthesis of phtocatalytically active and biocompatible TiO2 and ZnO nanoparticles0 aBacterial synthesis of phtocatalytically active and biocompatibl cDEC a80-990 v23 a

TiO2 and ZnO nanoparticles are well known for their photocatalytic and ultraviolet (UV)-absorbent properties and have successfully entered into the market as consumer products. Large volume production of these nanoparticles by industries require environmentally friendly processes of synthesis. In this article, we show that when challenged with an appropriate precursor, the bacterium Actinobacter spp. can lead to the extracellular synthesis of ZnO and TiO2 nanoparticles. The biological way of synthesizing these materials probably leads to the in situ doping of elements like C, F, and N into their crystal lattices, which is evidenced by a red shift in the absorption edge. Because the doping is expected to affect the photocatalytic activity of these materials under different light conditions, such investigations have also been undertaken here. Further, the biocompatibility of these nanomaterials (cytotoxicity and genotoxicity) has also been investigated in detail.

10aBiosynthesis10agenotoxicity10aNanoparticles10aNanotoxicity10aPhotocatalysis1 aSingh, Sanjay1 aBritto, Virginia, D.1 aBharde, Atul, A.1 aSastry, Murali1 aDhawan, Alok1 aPrasad, Bhagavatula L. V. uhttps://library.ncl.res.in/content/bacterial-synthesis-phtocatalytically-active-and-biocompatible-tio2-and-zno-nanoparticles01710nas a2200217 4500008004100000022001400041245008800055210006900143260010600212300001400318490000700332520088500339653001601224653001201240653002801252653002001280653002101300100002501321700002401346856012201370 2010 eng d a0040-402000aBase dependent pyrrolidine ring pucker in aep-PNA monomers NMR and PSEUROT analysis0 aBase dependent pyrrolidine ring pucker in aepPNA monomers NMR an aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcNOV a9165-91700 v663 a

The aep-PNA is a chiral and cyclic PNA analogue which has a stronger and base dependent binding affinity with complementary DNA To understand the base dependent properties at monomer level the structural studies of aep-PNA-(T/C/A) monomers have been carried out focussing on the conformational analysis of pyrrolidine ring pucker in aep-PNA by H-1 NMR and the coupling constant data fitted into PSEUROT software The results indicate that the type of pyrrolidine pucker depends on the electronic nature of substituent implying the effect of pyrimidine or purine substituents in determining the ring pucker in monomers This may consequently influence the aep-PNA oligomer conformation Since pyrrolidine nucleic acids have emerged as an important class of PNA analogues present results may have importance for their future development (C) 2010 Elsevier Ltd All rights reserved

10aA T G and C10aaep PNA10aConformational analysis10aDNA RNA and PNA10aPyrrolidine ring1 aSharma, Nagendra, K.1 aGanesh, Krishna, N. uhttps://library.ncl.res.in/content/base-dependent-pyrrolidine-ring-pucker-aep-pna-monomers-nmr-and-pseurot-analysis-000802nas a2200205 4500008004100000245008000041210006900121260000800190100002000198700003100218700003100249700003100280700002800311700003300339700002500372700004300397700002200440700001900462856011500481 2010 eng d00aBeaded cross linked polymers containing tert-amino functional group moities0 aBeaded cross linked polymers containing tertamino functional gro cDEC1 aDwivedi, Mayank1 aRao, Locanindi, Hari Sarvo1 aReddy, Krishna, Mohan Srin1 aDhanasekharan, Janakiraman1 aRao, Bevara, Madhusudan1 aKumar, Sriperambudur, Rajesh1 aPonrathnam, Surendra1 aChelanattukizhakkemadath, Raman, Rajan1 aTayal, Rajivkumar1 aQureshi, Moham uhttps://library.ncl.res.in/content/beaded-cross-linked-polymers-containing-tert-amino-functional-group-moities00442nas a2200133 4500008004100000022001400041245002300055210002300078260010200101300000600203490000700209100003200216856006000248 2010 eng d a0011-389100aBeyond irreverence0 aBeyond irreverence aC V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIAbINDIAN ACAD SCIENCEScJUL a70 v991 aMashelkar, Raghunath, Anant uhttps://library.ncl.res.in/content/beyond-irreverence-001982nas a2200241 4500008004100000022001400041245016500055210006900220260009900289300001400388490000800402520103100410653001801441653001201459653001801471653002601489653002501515100001801540700001801558700002101576700001401597856012901611 2010 eng d a0960-852400aBiocatalytic approach for the utilization of hemicellulose for ethanol production from agricultural residue using thermostable xylanase and thermotolerant yeast0 aBiocatalytic approach for the utilization of hemicellulose for e aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJUL a5366-53730 v1013 a

A hydrolysis of 62% and 50% for OSX (Oat spelt xylan) and WBH (Wheat bran hemicellulose) were obtained in 36 h and 48 h using Accellerase(TM) 1000 at 50 degrees C wherein thermostable xylanase from alkalothermophilic Thermomonospora sp. yielded 67% (OSX) in 3 h and 58% (WBH) in 24 h at 60 degrees C, favouring a reduction in process time and enzyme dosage. The rate of hydrolysis with thermostable xylanase was increased by 20% with the addition of nonionic surfactant tween 80 or biosurfactant sophorolipid. The simultaneous saccharification and fermentation (SSF) of OSX and WBH using thermostable xylanase and D. hansenii in batch cultures produced 9.1 g/L and 9.5 g/L of ethanol, respectively and had a shorter overall process time than the separate hydrolysis and fermentation (SHF). The immobilized yeast cells in Ca-alginate matrix produced ethanol with a yield of 0.46 g/g from hemicellulosic hydrolysates and were reused six times with 100% fermentation efficiency. (C) 2010 Elsevier Ltd. All rights reserved.

10aBiosurfactant10aEthanol10aHemicellulose10aThermostable xylanase10aThermotolerant yeast1 aMenon, Vishnu1 aPrakash, Gyan1 aPrabhune, Asmita1 aRao, Mala uhttps://library.ncl.res.in/content/biocatalytic-approach-utilization-hemicellulose-ethanol-production-agricultural-residue-001595nas a2200181 4500008004100000022001400041245014400055210006900199260011000268300001400378490000700392520078800399100002501187700002101212700002401233700003001257856012601287 2010 eng d a0959-942800aBio-inspired catalyst compositions for enhanced oxygen reduction using nanostructured Pt electrocatalysts in polymer electrolyte fuel cells0 aBioinspired catalyst compositions for enhanced oxygen reduction  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcSEP a9651-96570 v203 a

Composites of Nafion with a class of bio-molecules viz., plant hormones, are explored as potential polymer electrolytes for improving the proton transport inside the catalyst layer of a H(2)/O(2) fuel cell. Specifically, four nitrogenous plant hormones, two each from the class of auxins and cytokinins have been investigated, following preliminary characterization of the composite dispersions and membranes. Interestingly, the use of indole-3-acetic acid (an auxin) in the catalyst layer reveals a 30% enhancement in Pt catalyst utilization and improved fuel cell performance by 150 mW cm(-2). The effect of these bio-molecules on the kinetic and mass transport parameters has been analyzed systematically using a combination of electrochemical and spectroscopic techniques.

1 aParthasarathy, Meera1 aKannan, Ramaiyan1 aSreekumar, Kurungot1 aPillai, Vijayamohanan, K. uhttps://library.ncl.res.in/content/bio-inspired-catalyst-compositions-enhanced-oxygen-reduction-using-nanostructured-pt-000547nas a2200133 4500008004100000022001400041245007300055210006900128260007900197300000700276490000700283100001700290856010600307 2010 eng d a1054-252300aBiotechnology of endophytic fungi: inorganic nanomaterials and drugs0 aBiotechnology of endophytic fungi inorganic nanomaterials and dr a675 MASSACHUSETTS AVE, CAMBRIDGE, MA 02139 USAbBIRKHAUSER BOSTON INCcFEB aS30 v191 aAhmad, Absar uhttps://library.ncl.res.in/content/biotechnology-endophytic-fungi-inorganic-nanomaterials-and-drugs-001300nas a2200205 4500008004100000022001400041245014600055210006900201260007500270300001400345520045900359653002600818653001200844653001600856653003200872653002100904100002200925700001900947856012800966 2010 eng d a0039-788100aBromine-induced facile synthesis of butenolides and spirobutenolides from sterically congested tetrasubstituted dialkyl alkylidene succinates0 aBromineinduced facile synthesis of butenolides and spirobutenoli aRUDIGERSTR 14, D-70469 STUTTGART, GERMANYbGEORG THIEME VERLAG KGcAPR a1188-11943 a

Starting from sterically congested tetrasubstituted dialkyl alkylidene succinates, facile general approach to several dialkyl substituted butenolides and spirobutenolides with the generation of quaternary carbon center has been demonstrated via bromine-induced dealkylative regioselective intramolecular cyclization and dehydrobromination pathway. The mechanistic aspects involved in the formation of butenolides have been also described in brief.

10aalkylidene succinates10abromine10abutenolides10aintramolecular cyclizations10aspirobutenolides1 aPatel, Ramesh, M.1 aArgade, N., P. uhttps://library.ncl.res.in/content/bromine-induced-facile-synthesis-butenolides-and-spirobutenolides-sterically-congested-001559nas a2200193 4500008004100000022001400041245013000055210006900185260011000254300001400364490000600378520072500384100002201109700002301131700002601154700002601180700002801206856013101234 2011 eng d a1477-052000aBicyclic amino acid-carbohydrate-conjugates as conformationally restricted hydroxyethylamine (HEA) transition-state isosteres0 aBicyclic amino acidcarbohydrateconjugates as conformationally re aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcSEP a7300-73020 v93 a

This communication describes a general synthetic route to bicyclic amino acid-carbohydrate-conjugates, which would be useful as conformationally restricted hydroxyethylamine (HEA) transition-state isosteres. The synthesis was achieved in 12 steps starting from D-glucose. The striking features of this system are the bicyclic rigid core displaying an a-amino acid side chain and hydroxyethylamine moiety - both of which would be potentially important for receptor interactions, leading to various biomedical responses, as described in the literature. Crystal structure investigation suggested extensive intermolecular hydrogen-bonding interactions in this system, involving the backbone amide and hydroxyl groups.

1 aKale, Sangram, S.1 aChavan, Sanjay, T.1 aSabharwal, Sushma, G.1 aPuranik, Vedavati, G.1 aSanjayan, Gangadhar, J. uhttps://library.ncl.res.in/content/bicyclic-amino-acid-carbohydrate-conjugates-conformationally-restricted-hydroxyethylamine-000855nas a2200217 4500008004100000022001400041245008100055210006900136260010600205300001400311490000700325520005700332653000600389653001500395653002500410653002200435653001500457100002500472700002800497856011200525 2011 eng d a0040-403900aBidentate ligand-catalyzed enantioselective addition of RZnX to benzaldehyde0 aBidentate ligandcatalyzed enantioselective addition of RZnX to b aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcNOV a6501-65030 v523 a

{Enantioselective addition of RZnX (R = alkyl

10a110a2-Addition10aAsymmetric synthesis10aChelating ligands10aOrganozinc1 aJagtap, Ravindra, S.1 aJoshi, Navalkishore, N. uhttps://library.ncl.res.in/content/bidentate-ligand-catalyzed-enantioselective-addition-rznx-benzaldehyde-002157nas a2200181 4500008004100000022001400041245010000055210006900155260005700224300001200281490000800293520145200301100002501753700002301778700002101801700002201822856013101844 2011 eng d a0932-011300aBioactivity of essential oils of zingiber officinalis and achyranthes aspera against mosquitoes0 aBioactivity of essential oils of zingiber officinalis and achyra a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcAUG a339-3430 v1093 a

Due to the global health problems associated with mosquito-borne diseases, over two million people primarily in the tropical countries are at risk. The widely and commonly used chemical method though effective, has some major disadvantages making insect control practically difficult. In view of the above, it is unavoidable to search for new molecules, which are eco-friendly, cheaper, and safer. The present study deals with evaluation of bioactive potential of two commonly occurring plants against mosquitoes presenting an alternative to the conventional chemical methods. Essential oils extracted by steam distillation from rhizome of Zingiber officinalis and leaf and stem of Achyranthes aspera were evaluated for larvicidal, attractant/repellent, and oviposition attractant/deterrent activity against two mosquito species viz. Aedes aegypti and Culex quinquefasciatus. The highest larvicidal activity, i.e., LC(50) = 154 ppm and LC(50) = 197 ppm for A. aegypti and C. quinquefasciatus, respectively was shown by Z. officinalis. This oil also offers 5-h protection at the concentration of 0.5 mg/cmA(2) from both mosquito species. The highest oviposition deterrence activity was exhibited by A. aspera stem oil, i.e., 100% and 85.71%, in case of A. aegypti and C. quinquefasciatus, respectively, at the concentration of 0.1%. These results reveal that both these oils have control potential against A. aegypti and C. quinquefasciatus.

1 aKhandagle, Abhay, J.1 aTare, Vrushali, S.1 aRaut, Kishor, D.1 aMorey, Rashmi, A. uhttps://library.ncl.res.in/content/bioactivity-essential-oils-zingiber-officinalis-and-achyranthes-aspera-against-mosquitoes-002457nas a2200253 4500008004100000022001400041245017200055210006900227260006700296300001400363490000700377520145800384653002301842653002301865653001301888653002901901653002301930100002501953700002801978700002002006700002302026700003002049856012402079 2011 eng d a0022-514200aBiochemical characterisation of alpha-amylase inhibitors from achyranthes aspera and their interactions with digestive amylases of coleopteran and lepidopteran insects0 aBiochemical characterisation of alphaamylase inhibitors from ach a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcAUG a1773-17800 v913 a

BACKGROUND: Starchy seeds are an important food and a source of dietary ingredients in many countries. However, they suffer from extensive predation by bruchids (weevils) and other pests. alpha-Amylase inhibitors are attractive candidates for the control of seed weevils, as these insects are highly dependent on starch as an energy source. RESULTS: A proteinaceous alpha-amylase inhibitor from the seeds of Achyranthes aspera was identified, purified and characterised. In electrophoretic analysis, two prominent amylase inhibitor activity bands (AI1 and AI2) were detected. The inhibitor was purified 9.99-fold with 1206.95 total amylase inhibitor units mg(-1) protein. The molecular weight of the purified inhibitor was around 6 kDa. The isolated alpha-amylase inhibitor was found to be resistant to heat and proteolysis. Feeding analysis of Callosobruchus maculatus larvae on a diet containing seed powder of A. aspera revealed that survival of the larvae was severely affected, with the highest mortality rate occurring on the fifth day of feeding. The isolated inhibitor inhibited the majority of amylase isoforms of C. maculatus, Tribolium confusum and Helicoverpa armigera in electrophoretic analysis and solution assays. CONCLUSION: The information obtained in the present investigation could be useful for a genetic engineering approach that would make seeds resistant to storage pest infestations. (C) 2011 Society of Chemical Industry

10aAchyranthes aspera10aAmylase inhibitors10aamylases10aCallosobruchus maculatus10aTribolium confusum1 aHivrale, Vandana, K.1 aChougule, Nanasaheb, P.1 aGiri, Ashok, P.1 aChhabda, Pavan, J.1 aKachole, Andmanvendra, S. uhttps://library.ncl.res.in/content/biochemical-characterisation-alpha-amylase-inhibitors-achyranthes-aspera-and-their-002299nas a2200265 4500008004100000022001400041245010800055210006900163260007600232300001200308490000700320520135100327653001501678653002001693653001501713653002301728653002201751100001801773700001701791700002701808700001901835700003001854700002201884856012701906 2011 eng d a0260-437X00aBiocompatible gellan gum-reduced gold nanoparticles: cellular uptake and subacute oral toxicity studies0 aBiocompatible gellan gumreduced gold nanoparticles cellular upta aCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USAbWILEY-BLACKWELLcJUL a411-4200 v313 a

Currently gold nanoparticles are being explored for drug delivery and other biomedical applications; therefore it is necessary to study the fate of such nanoparticles inside the body. The objective of the present study was to investigate the cellular uptake and toxicity of the gold nanoparticles synthesized using a microbial polysaccharide, gellan gum, as a capping and reducing agent. The cellular uptake of gold nanoparticles was studied on mouse embryonic fibroblast cells, NIH3T3 and human glioma cell line, LN-229. The cellular uptake study indicated that the gellan gum-reduced gold nanoparticles were located in cancer cells (LN-229) while no uptake was observed in normal mouse embryonic fibroblast cells (NIH3T3). The toxicity of the gold nanoparticles was evaluated by carrying out subacute 28 day oral toxicity studies in rats. Subacute administration of gum-reduced gold nanoparticles to the rats did not show any hematological or biochemical abnormalities. The weight and normal architecture of various organs did not change compared with control. The current findings, while establishing the specific uptake of nanoparticles into cancerous cells, also demonstrates that the gellan gum-reduced gold nanoparticles are devoid of toxicity in animals following oral administration. Copyright (c) 2010 John Wiley & Sons, Ltd.

10acell lines10acellular uptake10aGellan gum10agold nanoparticles10asubacute toxicity1 aDhar, Sheetal1 aMali, Vishal1 aBodhankar, Subhash, L.1 aShiras, Anjali1 aPrasad, Bhagavatula L. V.1 aPokharkar, Varsha uhttps://library.ncl.res.in/content/biocompatible-gellan-gum-reduced-gold-nanoparticles-cellular-uptake-and-subacute-oral-002012nas a2200229 4500008004100000022001400041245010700055210006900162260007500231300001200306490000700318520115700325653001401482653002501496653001901521653001501540653002501555653002401580100002101604700002801625856012901653 2011 eng d a1571-101300aBiodiesel production from vegetable oils and animal fat over solid acid double-metal cyanide catalysts0 aBiodiesel production from vegetable oils and animal fat over sol a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGER/PLENUM PUBLISHERScSEP a145-1600 v153 a

Biodiesel comprises of fatty acid alkyl esters prepared from vegetable oils or animal fat by esterification/transesterification with short-chain alcohols (methanol or ethanol, for example). It is a biodegradable renewable fuel. Its production is growing exponentially due to greater concerns about environmental protection and depletion of fossil fuel resources. Further, its production from non-edible oils and animal fat is more desirable than from edible oils due to lower cost of non-edible feedstocks and elimination of food verses fuel issues. Solid acid catalysts are ideal for conversion of such low-grade oils to biodiesel. Biodiesel from non-edible oils can be produced by two methods: (1) simultaneous esterification of fatty acids and transesterification of fatty acid glycerides and (2) hydrolysis of glycerides followed by esterification. This account reports the catalytic performance of solid, Fe-Zn double-metal cyanide (DMC) complexes and other acid catalysts in these transformations for biodiesel production. The factors influencing the catalytic performance of the solid acid catalysts in biodiesel production are discussed.

10aBiodiesel10aDouble-metal cyanide10aEsterification10aHydrolysis10asolid acid catalysts10atransesterification1 aSrinivas, Darbha1 aSatyarthi, Jitendra, K. uhttps://library.ncl.res.in/content/biodiesel-production-vegetable-oils-and-animal-fat-over-solid-acid-double-metal-cyanide-002048nas a2200241 4500008004100000022001400041245014500055210006900200260007300269300001200342490000700354520117600361653001201537653001501549653001301564653001401577653001401591653002501605100001801630700001901648700001401667856012501681 2011 eng d a0378-382000aBioethanol production from renewable polymer lichenan using lichenase from an alkalothermophilic thermomonospora sp and thermotolerant yeast0 aBioethanol production from renewable polymer lichenan using lich aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR a401-4060 v923 a

Biomass feedstocks available decentrally will be more commodious for localized biorefinery approach than the exhaustive large scale and centralized plants driven by cost intensive technology. Lichen is present in a wide range of habitats in a distributed manner. A maximum hydrolysis of 73%-76% for lichenan from Cetraria islandica, Usnea barbata and Parmelia sp. were obtained in 24 h using lichenase from an alkalothermophilic Thermomonospora sp. wherein the hydrolysis was 100% with commercial enzyme Accellerase (TM) 1000. The synergistic role of beta-glucosidase in lichenan hydrolysis was demonstrated by the exogenous addition of beta-glucosidase to Thermomonospora lichenase which resulted in complete hydrolysis. The hydrolysates of lichenan obtained using Accellerase or a cocktail of Thermomonospora lichenase and beta-glucosidase when fermented with free cells of Saccharomyces at 40 degrees C produced an ethanol yield of 0.45 g/g-0.48 g/g with theoretical conversion efficiencies of 93%-96%. The Ca-alginate immobilized yeast cells were reused eight times at 40 degrees C with 100% fermentation efficiency. (C) 2010 Elsevier B.V. All rights reserved.

10aEthanol10aHydrolysis10aLichenan10aLichenase10aSynergism10aThermotolerant yeast1 aMenon, Vishnu1 aDivate, Rupesh1 aRao, Mala uhttps://library.ncl.res.in/content/bioethanol-production-renewable-polymer-lichenan-using-lichenase-alkalothermophilic-001996nas a2200277 4500008004100000022001400041245012500055210006900180260009400249300001400343490000700357520097500364653002301339653002201362653002501384653001201409653002201421100002801443700002001471700001801491700001901509700001601528700001801544700002701562856012901589 2011 eng d a1684-531500aBiogenic synthesis of silver nanoparticles using nicotiana tobaccum leaf extract and study of their antibacterial effect0 aBiogenic synthesis of silver nanoparticles using nicotiana tobac aP O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIAbACADEMIC JOURNALScAUG a8122-81300 v103 a

A green synthesis of silver nanoparticle was carried out using tobacco leaf extract. Synthesized nanoparticles were characterized using UV-Vis absorption spectroscopy, TEM, EDAX, FT-IR and photoluminescence study, respectively. UV-Vis absorption spectroscopy of prepared silver colloidal solution showed absorption maxima at 418 nm. Excitation maximum and emission maximum obtained from photoluminescence study were found at 414 and 576 nm, respectively. TEM analysis showed average particle size of 8 nm, while SAED pattern confirmed the crystalline nature of synthesized nanoparticles. FT-IR analysis indicated the involvement of carboxyl (-C = O), hydroxyl (-OH) and amine (-NH) functional groups of tobacco leaf extract in preparation of silver nanoparticles. EDAX analysis showed proportion of silver (54.55%) among other elements in nanoparticle. Pseudomonas aeruginosa and Escherichia coli DH5 alpha showed highest sensitivity towards silver nanoparticles.

10aFT-IR spectroscopy10aphotoluminescence10asilver nanoparticles10aTobacco10aUV-Vis absorption1 aPrasad, Kumar, Suranjit1 aPathak, Darshit1 aPatel, Ankita1 aDalwadi, Palak1 aPrasad, Ram1 aPatel, Pradip1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/biogenic-synthesis-silver-nanoparticles-using-nicotiana-tobaccum-leaf-extract-and-study-001971nas a2200193 4500008004100000022001400041245011300055210006900168260009100237300001200328490000600340520117000346653002601516653001901542653004401561100001801605700002901623856012501652 2011 eng d a0973-751000aBiological control of cyanobacteria using polyphosphate accumulating bacteria isolated from activated sludge0 aBiological control of cyanobacteria using polyphosphate accumula a54, NEAR POST OFFICE, THANA ST, BHOPAL, SHAHJAHANABAD 462 001, INDIAbDR M N KHANcAPR a469-4710 v53 a

Increased input of nutrients into water body leads to massive proliferation of cyanobacteria and other algae. This ultimately results in the eutrophication of the water body. The cyanobacterial blooms exert negative environmental impacts such as extensive growth of alga, bad odor, decline in dissolved oxygen, production of cyanotoxin, decreased growth of submerged aquatic organisms and change in taste of water. This results into reduction in qualitative and quantitative properties of water. Various chemical based methods of eutrophication control are suggested by researchers but those endanger the aquatic ecosystem. Considering hazardous effect of chemical agents, biological agents may be used more safely. Here we have shown that polyphosphate accumulating bacteria (PABs) isolated from activated sludge are efficient in reducing cyanobacterial blooms up to 95.56% by trapping phosphorus in metapolyphosphate form. This is the first study that provides direct evidence that use of these microorganisms gives promising result in controlling cyanobacterial blooms and consequently eutrophication process without affecting the ecosystem of water body.

10aCyanobacterial blooms10aEutrophication10aMetapolyphosphate accumulating bacteria1 aDudhane, Amol1 aMhaindarkar, Vaibhav, P. uhttps://library.ncl.res.in/content/biological-control-cyanobacteria-using-polyphosphate-accumulating-bacteria-isolated-000895nas a2200169 4500008004100000022001400041245005400055210005400109260007100163300001400234490000800248520031600256100002600572700002200598700001700620856008800637 2011 eng d a0002-786300aBiomimetic total syntheses of flinderoles B and C0 aBiomimetic total syntheses of flinderoles B and C a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcMAR a2864-28670 v1333 a

A simple and efficient biomimetic synthesis of pyrrolo[1,2-a]indoles using a highly stereo- and regioselective [3 + 2] reaction cascade was developed and then further applied in the first total synthesis of flinderoles B and C, which proceeded in 17.2% yield over the longest linear sequence of 11 steps.

1 aDethe, Dattatraya, H.1 aErande, Rohan, D.1 aRanjan, Alok uhttps://library.ncl.res.in/content/biomimetic-total-syntheses-flinderoles-b-and-c-000699nas a2200205 4500008004100000245007700041210006900118260000800187300001600195490000800211100001700219700002400236700001200260700001500272700002300287700002600310700002400336700002500360856010800385 2011 eng d00aBioresponsive mesoporous silica nanoparticles for triggered drug release0 aBioresponsive mesoporous silica nanoparticles for triggered drug cDEC a19582-195850 v1331 aSingh, Neetu1 aKarambelkar, Amrita1 aGu, Luo1 aLin, Kevin1 aMiller, Jordan, S.1 aChen, Christopher, S.1 aSailor, Michael, J.1 aBhatia, Sangeeta, N. uhttps://library.ncl.res.in/content/bioresponsive-mesoporous-silica-nanoparticles-triggered-drug-release01050nas a2200169 4500008004100000245008200041210006900123260000800192300001600200490000700216520046600223100002600689700002000715700001700735700002300752856010500775 2011 eng d00aBorazine as a sensor for fluoride ion: a computational and experimental study0 aBorazine as a sensor for fluoride ion a computational and experi cMAY a3636–36390 v523 a

The computational and experimental studies have revealed that even simple molecule like borazine can act as a sensor for fluoride ions. This study further reported the various binding modes of analytes using quantum chemical calculations and the nature of such interactions have been examined using electron density surface analysis. Total charge transfer analysis (qCT) correlates well with the binding affinities of analytes with the borazine receptor.

1 aKesharwani, Manoj, K.1 aSuresh, Moorthy1 aDas, Amitava1 aGanguly, Bishwajit uhttps://library.ncl.res.in/content/borazine-sensor-fluoride-ion-computational-and-experimental-study01836nas a2200181 4500008004100000022001400041245006900055210006900124260011000193300001400303490000700317520112700324100002601451700002501477700002001502700003001522856010201552 2012 eng d a1463-907600aBand edge modulated conjugated polymers for oxidation prevention0 aBand edge modulated conjugated polymers for oxidation prevention aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcFEB a4577-45830 v143 a

The impact of electron transfer (ET) from a series of band edge modulated polymers to atmospheric oxygen is examined in connection with substrate oxidation prevention. Polymers with the highest occupied molecular orbital (HOMO) energy level below and above the oxygen energy level were tested and the former showed better efficiency. Furthermore, the oxidation prevention efficiency of a polymer with lower HOMO increased by two orders of magnitude, when the pores on the film were filled with spherical molecules, [6,6]-phenyl-C61-butyric acid methyl ester. We found that the polymer surface hydrophobicity has little or no influence on oxidation prevention. It is interesting to note that a polymer with a hole mobility of 8 x 10(-10) cm(2) V-1 s(-1) showed a two-fold increase in oxidation prevention efficiency compared to a polymer with a hole mobility of 6 x 10(-5) cm(2) V-1 s(-1). Over all, from the concerted approach, we conclude that a polymer devoid of pores with the HOMO energy level below oxygen and low charge carrier mobility is a suitable candidate for prevention of substrate oxidation/corrosion.

1 aMahale, Rajashree, Y.1 aArulkashmir, Arulraj1 aDutta, Kingshuk1 aKrishnamoorthy, Kothandam uhttps://library.ncl.res.in/content/band-edge-modulated-conjugated-polymers-oxidation-prevention-001322nas a2200181 4500008004100000022001400041245014600055210006900201260011000270300001600380490000600396520050000402100003100902700002500933700002700958700002400985856013101009 2012 eng d a2046-206900aBase promoted highly efficient copper fluorapatite catalyzed coupling of phenols with arylboronic acids under mild and ligand-free conditions0 aBase promoted highly efficient copper fluorapatite catalyzed cou aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcOCT a12818-128230 v23 a

A mild, general and highly efficient protocol has been developed for the synthesis of diaryl ethers in good to excellent yield under mild and ligand-free conditions. This is the first example in which a recyclable, heterogeneous copper fluorapatite catalyst is used for the arylation of phenols with arylboronic acids at room temperature in the presence of Cs2CO3 as a base and methanol as a solvent. The catalyst was recovered and reused several times without loss of catalytic activity.

1 aMulla, Shafeek, Abdul Rash1 aInamdar, Suleman, M.1 aPathan, Mohsinkhan, Y.1 aChavan, Santosh, S. uhttps://library.ncl.res.in/content/base-promoted-highly-efficient-copper-fluorapatite-catalyzed-coupling-phenols-arylboronic-001862nas a2200265 4500008004100000022001400041245014100055210006900196260015900265300001200424490000700436520082700443653001901270653002901289653001601318653002301334653001401357653001301371653001501384653001501399653002201414653001201436100002301448856012501471 2012 eng d a1857-555200aBehavior of the transport and separation of lanthanum, yttrium and lutetium metal ions through a celluose fiber supported solid membrane0 aBehavior of the transport and separation of lanthanum yttrium an aSTS CYRIL & METHODIUS UNIV, FAC TECHNOL & METALLURGY, PO BOX 560, RUGER BOSKOVIC 16, SKOPJE, MK-1001, MACEDONIAbSOC CHEMISTS TECHNOLOGISTS MADECONIAcJan a255-2690 v313 a

A newly designed membrane cell was used to study the behavior of the transport and separation of lanthanum, yttrium and lutetium metal ions using a cellulose fiber supported solid membrane. The cellulose fiber membrane was prepared by the chemical modification of cellulose fiber using an esterification reaction with citric acid. Different experimental variables were investigated, such as time, membrane size, stirring of the source and receiving phases and the pH of the source phase. The use of different stripping agents in the receiving phase was explored, including nitric acid, hydrochloric acid, sodium nitrate, ammonium thiocyanate, D(2)EHPA, TBP, Aliquat-336, tartaric acid, EDTA and organic solvents. Pre-concentration of lanthanum, yttrium and lutetium metal ions from dilute solutions was carried out.

10abinary mixture10acellulose fiber membrane10aCitric acid10acomplexing reagent10aLanthanum10alutetium10ametal ions10aSeparation10atransport studies10ayttrium1 aGaikwad, Abaji, G. uhttps://library.ncl.res.in/content/behavior-transport-and-separation-lanthanum-yttrium-and-lutetium-metal-ions-through-002149nas a2200229 4500008004100000022001400041245011300055210006900168260005700237300000900294490000800303520130600311653000801617653003301625653003701658653002301695653001301718100002301731700002201754700001601776856012701792 2012 eng d a1432-881X00aBehaviour of density functional theory for electric response properties at distorted geometries of molecules0 aBehaviour of density functional theory for electric response pro a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcFEB a10940 v1313 a

The role of exchange-correlation is well known for accurate calculations of electric response properties. The exchange-correlation functional in density functional theory (DFT) has been well studied for ground state equilibrium geometry. However, the behaviour of these functional in stretched geometries, where static correlation play an important role, has not been studied systematically, particularly for response electric properties. Thus, we present here the rigorous calculation of electric response properties at distorted geometries of the molecules. We have considered dipole polarizability and dipole-quadrupole polarizability for description of role of static and dynamic correlation for electric response properties. The calculations are performed with our new approach, noniterative approximation to coupled-perturbed Kohn-Sham method. These DFT results are compared with higher level ab initio such as coupled perturbed singles and doubles and fully correlated full CI. We have studied single, double and triple-bonded systems at different inter-nuclear separation. We report here the dipole polarizability and dipole-quadrupole polarizability of HF, BH, H2CO, CO and NO+. We also present the effect of basis and functional on polarizability and dipole-quadrupole polarizability.

10aDFT10aDipole-dipole polarizability10aDipole-quadrupole polarizability10aDistorted geometry10aNIA-CPKS1 aShedge, Sapana, V.1 aJoshi, Sayali, P.1 aPal, Sourav uhttps://library.ncl.res.in/content/behaviour-density-functional-theory-electric-response-properties-distorted-geometries-001706nas a2200217 4500008004100000022001400041245013400055210006900189260007300258300001200331490000800343520084800351653002901199653003401228653003301262653001801295653001601313100001801329700001801347856012301365 2012 eng d a0920-586100aBinapthyl Schiff base diamine complex covalently bonded to modified SBA-15: synthesis, characterization and catalytic application0 aBinapthyl Schiff base diamine complex covalently bonded to modif aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcDEC a184-1880 v1983 a

Protected mesoporous SBA-15 phase was synthesized by grafting the complex Chloro (S,S)(-)[N-3-tert-butyl-5-chloromethyl salicylidene]-N'-[3',5'-di tert-butyl salicylidene] 1,1'-binapthyl-2,2'-diamine manganese(III) through the reactive 3-aminopropyl trimethoxysilane(3-APTMS) group. The surface properties of the functionalized catalyst were analyzed by a series of characterization techniques like elemental analysis, XRD, N-2 sorption measurement isotherm, FT-IR, XPS, and solid state C-13 NMR. The screening of the catalyst Mn(III)-L-SBA-15 and neat Mn(III)-L complexes was done for the oxidation reaction of thioanisole (methyl phenyl sulfide) using TBHP as an oxidant. Mn(III)-L-SBA-15 catalyst shows higher activities and exhibit enantiomeric excess comparable to homogeneous catalyst. (C) 2012 Elsevier B. V. All rights reserved.

10a-OH protected NH2-SBA-1510aBinaphthyl Schiff base ligand10aOrdered mesoporous materials10aSulfoxidation10aThioanisole1 aSharma, Priti1 aSingh, A., P. uhttps://library.ncl.res.in/content/binapthyl-schiff-base-diamine-complex-covalently-bonded-modified-sba-15-synthesis-000684nas a2200181 4500008004100000245011100041210006900152260000800221300001200229490000600241100001900247700002100266700001900287700001900306700001700325700002500342856013500367 2012 eng d00aBioanalytical method development and validation of levalbuterol a β2-adrenergic agonist by RP-HPLC method0 aBioanalytical method development and validation of levalbuterol  cMAR a249-2530 v41 aGhemud, A., S.1 aSanthakumari, B.1 aPharne, A., B.1 aJadhav, M., M.1 aJain, K., S.1 aKulkarni, Mahesh, J. uhttps://library.ncl.res.in/content/bioanalytical-method-development-and-validation-levalbuterol-%CE%B22-adrenergic-agonist-rp-hplc01823nas a2200181 4500008004100000245012800041210006900169260000800238300001200246490000600258520114600264100001901410700002101429700001901450700001701469700002501486856013001511 2012 eng d00aBioanalytical method development and validation of vildagliptin a novel dipeptidyl peptidase IV inhibitor by RP-HPLC method0 aBioanalytical method development and validation of vildagliptin  cJUN a119-1230 v43 a

A simple, selective, rapid, precise and economical Reverse-Phase HPLC method has been developed and validated for quantitative determination of vildagliptin in plasma. Vildagliptin is a potent dipeptidyl peptidase IV inhibitor used for treatment of diabetes. Tolbutamide is used as an internal standard. The method was carried out with UV Spectrophotometric detection using a Perkin Elmer Series 200 HPLC system equipped with XBridge Shield C18 column (3.5 μm, 4.6x150mm) and a guard column of the same type, at a flow rate of 1.0mL/min. Detection was carried out at 210 nm. The mobile phase consisted of 50mM ammonium bicarbonate (pH 7.8) (solvent A) and acetonitrile (solvent B). The retention times of vildagliptin and tolbutamide were 11.2 min and 13.4 min respectively. The method was developed and tested for linearity range of 10μg/ml to 120μg/ml. The developed method was validated in terms of selectivity, accuracy, precision, linearity, and stability study. The proposed method uses less biological material and the method is MS compatible also. Method can be applicable for pharmacokinetic studies using HPLC or LC-MS.

1 aPharne, A., B.1 aSanthakumari, B.1 aGhemud, A., S.1 aJain, H., K.1 aKulkarni, Mahesh, J. uhttps://library.ncl.res.in/content/bioanalytical-method-development-and-validation-vildagliptin-novel-dipeptidyl-peptidase-iv02277nas a2200229 4500008004100000022001400041245011800055210006900173260010700242300001200349490000700361520138000368653004301748653003401791653001701825653001701842653001801859100001601877700001801893700001401911856012201925 2012 eng d a1431-065100aBiochemical characterization of a novel thermostable xyloglucanase from an alkalothermophilic thermomonospora sp.0 aBiochemical characterization of a novel thermostable xyloglucana aCHIYODA FIRST BLDG EAST, 3-8-1 NISHI-KANDA, CHIYODA-KU, TOKYO, 101-0065, JAPANbSPRINGER JAPAN KKcJAN a135-1460 v163 a

Xyloglucanase from an extracellular culture filtrate of alkalothermophilic Thermomonospora sp. was purified to homogeneity with a molecular weight of 144 kDa as determined by SDS-PAGE and exhibited specificity towards xyloglucan with apparent K (m) of 1.67 mg/ml. The enzyme was active at a broad range of pH (5-8) and temperatures (40-80A degrees C). The optimum pH and temperature were 7 and 70A degrees C, respectively. The enzyme retained 100% activity at 50A degrees C for 60 h with half-lives of 14 h, 6 h and 7 min at 60, 70 and 80A degrees C, respectively. The kinetics of thermal denaturation revealed that the inactivation at 80A degrees C is due to unfolding of the enzyme as evidenced by the distinct red shift in the wavelength maximum of the fluorescence profile. Xyloglucanase activity was positively modulated in the presence of Zn2+, K+, cysteine, beta-mercaptoethanol and polyols. Thermostability was enhanced in the presence of additives (polyols and glycine) at 80A degrees C. A hydrolysis of 55% for galactoxyloglucan (GXG) from tamarind kernel powder (TKP) was obtained in 12 h at 60A degrees C and 6 h at 70A degrees C using thermostable xyloglucanases, favouring a reduction in process time and enzyme dosage. The enzyme was stable in the presence of commercial detergents (Ariel), indicating its potential as an additive to laundry detergents.

10aAlkalothermophilic Thermomonospora sp.10aBiotechnological applications10aPurification10aThermostable10aXyloglucanase1 aPol, Dipali1 aMenon, Vishnu1 aRao, Mala uhttps://library.ncl.res.in/content/biochemical-characterization-novel-thermostable-xyloglucanase-alkalothermophilic-001852nas a2200193 4500008004100000022001400041245010200055210006900157260011500226300000800341490000600349520109500355100001901450700001301469700001801482700001401500700001701514856012701531 2012 eng d a1750-044300aBiomimetic synthesis of selenium nanoparticles using cell-free extract of microbacterium sp ARB050 aBiomimetic synthesis of selenium nanoparticles using cellfree ex aMICHAEL FARADAY HOUSE SIX HILLS WAY STEVENAGE, HERTFORD SG1 2AY, ENGLANDbINST ENGINEERING TECHNOLOGY-IETcJAN a1-40 v73 a

This Letter is a novel approach to synthesise a bio-functionalised selenium nanoparticles using cell-free extract of selenium-resistant bacterial isolate. Phylogenetic analysis of isolate suggested its close similarity with Microbacterium luteolum (Y17235.1) and Microbacterium oxydans str. TPL09 (EU373379.1) while it was distantly related to Microbacterium dextranolyticum (Y17230.1). Selenium colloidal solution exhibited an absorption maximum at 300 nm and gave emission maximum at 590 nm. Transmission electron microscopy followed by selected area electron diffraction pattern analysis indicated the formation of spherical, polydispersed, crystalline, Se nanoparticles of diameter ranging from similar to 30 to 150 nm. X-ray diffraction results showed 111, 200 and 220 planes of face-centred cubic selenium. Energy dispersive analysis of X-rays confirmed the presence of selenium in nanosphere. Selenium nanoparticles synthesised in this manner can be studied for chemo-prevention as well as its fluorescent property can be utilised for molecular diagnostics in cancer research.

1 aPrasad, K., S.1 aVyas, P.1 aPrajapati, V.1 aPatel, P.1 aSelvaraj, K. uhttps://library.ncl.res.in/content/biomimetic-synthesis-selenium-nanoparticles-using-cell-free-extract-microbacterium-sp-001134nas a2200181 4500008004100000022001400041245010300055210006900158260007100227300001400298490000700312520043000319100002500749700001400774700001900788700002100807856012400828 2012 eng d a0020-166900aBistable dynamic coordination polymer showing reversible structural and functional transformations0 aBistable dynamic coordination polymer showing reversible structu a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcAUG a8317-83210 v513 a

A bistable dynamic coordination polymer [Ni(pca)(bdc)(0.5)(H2O)(2)] having a two-dimensional (2D) zigzag sheet structure is synthesized solvothermally. Topological analysis revealed that the frameworks have an hcb type of uninodal net. The compound exhibits guest specific reversible structural transformations accompanying reversible changes in physical properties driven by inherent flexibility and transformability.

1 aNagarkar, Sanjog, S.1 aDas, Raja1 aPoddar, Pankaj1 aGhosh, Sujit, K. uhttps://library.ncl.res.in/content/bistable-dynamic-coordination-polymer-showing-reversible-structural-and-functional-001552nas a2200217 4500008004100000245008600041210006900127260000800196300002700204490000800231520080500239100001901044700002301063700002401086700002101110700002401131700002101155700002401176700002101200856011301221 2013 eng d00aBand-like transport in high mobility unencapsulated single-layer MoS2 transistors0 aBandlike transport in high mobility unencapsulated singlelayer M cAPR aArticle Number: 1731070 v1023 aUltra-thin MoS2 has recently emerged as a promising two-dimensional semiconductor for electronic and optoelectronic applications. Here, we report high mobility (>60 cm(2)/Vs at room temperature) field-effect transistors that employ unencapsulated single-layer MoS2 on oxidized Si wafers with a low level of extrinsic contamination. While charge transport in the sub-threshold regime is consistent with a variable range hopping model, monotonically decreasing field-effect mobility with increasing temperature suggests band-like transport in the linear regime. At temperatures below 100 K, temperature-independent mobility is limited by Coulomb scattering, whereas, at temperatures above 100 K, phonon-limited mobility decreases as a power law with increasing temperature. (C) 2013 AIP Publishing LLC.1 aJariwala, Deep1 aSangwan, Vinod, K.1 aLate, Dattatray, J.1 aJohns, James, E.1 aDravid, Vinayak, P.1 aMarks, Tobin, J.1 aLauhon, Lincoln, J.1 aHersam, Mark, C. uhttps://library.ncl.res.in/content/band-transport-high-mobility-unencapsulated-single-layer-mos2-transistors01329nas a2200157 4500008004100000022001400041245009100055210006900146260010600215300001400321490000700335520065500342100002500997700002801022856012101050 2013 eng d a0957-416600aBase-catalyzed mukaiyama-type aldol additions, a continued quest for stereoselectivity0 aBasecatalyzed mukaiyamatype aldol additions a continued quest fo aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcNOV a1345-13630 v243 a

Base-catalyzed reactions of silyl enolates with carbonyl compounds are an attractive variant of conventional directed aldol reactions. Over the past two decades, the reaction has gained prominence due to its inherent advantage for stereoselective manipulations. The present review provides a brief account of developments in this area. Various approaches are grouped together in order to provide the salient features of each conceptual development. It appears that the methods which generate nascent chiral enolates or cationic siliconium species have the biggest influence on the stereoselectivity. (C) 2013 Elsevier Ltd. All rights reserved.

1 aSutar, Revannath, L.1 aJoshi, Navalkishore, N. uhttps://library.ncl.res.in/content/base-catalyzed-mukaiyama-type-aldol-additions-continued-quest-stereoselectivity-002291nas a2200361 4500008004100000022001400041245011000055210006900165260007300234300001000307490000800317520103700325653002401362653004201386653002401428653002001452100003101472700001901503700002401522700002501546700002201571700001501593700002201608700001701630700001801647700001901665700002201684700001901706700002501725700002901750700001901779856013101798 2013 eng d a0301-010400aBasic structural motif and major biophysical properties of Amyloid-beta are encoded in the fragment 18-350 aBasic structural motif and major biophysical properties of Amylo aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcAUG a80-870 v4223 a

Aggregation and misfolding of the amyloid beta (A beta) peptide is thought to initiate Alzheimer's disease (AD). Here we study the role played by its central segment (A beta(18-35)) in determining these properties. A beta(18-35) has a solubility of 18 mu M. The soluble fraction consists mainly of small oligomers, which have mixed beta-sheet and random coil structures. The monomer is mostly a random coil with some residual compactness. Aggregated A beta(18-35) forms fibrils of width 3.0 +/- 0.7 nm, which is consistent with a hairpin shape. Each of these properties has a close similarity to A beta(40). Remarkably, solid state NMR indicates that the fibrils also retain the secondary structure and tertiary contacts of A beta(40). This is the shortest fragment of A beta reported so far which preserves its fibrillar architecture, including the hairpin turn, as well as its solution phase conformational properties. Residues 18-35 should therefore be a key target of AD therapeutics. (C) 2013 Published by Elsevier B. V.

10aAlzheimer's disease10aFluorescence correlation spectroscopy10aProtein aggregation10aSolid-state NMR1 aChandrakesan, Muralidharan1 aSarkar, Bidyut1 aMithu, Venus, Singh1 aAbhyankar, Rajiv, M.1 aBhowmik, Debanjan1 aNag, Suman1 aSahoo, Bankanidhi1 aShah, Riddhi1 aGurav, Sushma1 aBanerjee, Raja1 aDandekar, Sucheta1 aJose, Jaya, C.1 aSengupta, Neelanjana1 aMadhu, Perunthiruthy, K.1 aMaiti, Sudipta uhttps://library.ncl.res.in/content/basic-structural-motif-and-major-biophysical-properties-amyloid-beta-are-encoded-fragment-001812nas a2200169 4500008004100000022001400041245011800055210006900173260011000242300001400352490000700366520106400373100001901437700002101456700003701477856012801514 2013 eng d a1463-926200aBiocatalysis: fungi mediated novel and selective 12 beta- or 17 beta-hydroxylation on the basic limonoid skeleton0 aBiocatalysis fungi mediated novel and selective 12 beta or 17 be aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcMAR a1311-13170 v153 a

Basic limonoids carrying a 4,4,8-trimethyl-17-furanylsteroid skeleton are a class of triterpenoids and well-known for their insecticidal as well as a vast array of pharmacological activities. Rare and synthetically challenging 12 beta- and 17 beta-hydroxylation was achieved on the basic limonoid skeleton to produce a novel series of hydroxylated limonoids using fungi-mediated biocatalysis. The fungal system belonging to the genera of Mucor efficiently converted azadiradione, epoxyazadiradione, gedunin and their derivatives into corresponding 12 beta- and/or 17 beta-hydroxy derivatives. The position and stereochemistry of hydroxylation was determined by rigorous spectroscopic and crystallographic studies. This fungi-mediated stereo- and regio-selective hydroxylation process was highly efficient and mild enough to sustain chemically sensitive functional groups around the basic limonoid skeleton. Modifications of specific functional groups and variation in biocatalyst were shown to bring selectivity among 12 beta- or 17 beta-hydroxylation.

1 aHaldar, Saikat1 aKolet, Swati, P.1 aThulasiram, Hirekodathakallu, V. uhttps://library.ncl.res.in/content/biocatalysis-fungi-mediated-novel-and-selective-12-beta-or-17-beta-hydroxylation-basic-001870nas a2200229 4500008004100000022001400041245010100055210006900156260007900225300001400304490000700318520099500325653002501320653002201345653001401367100002101381700002701402700001901429700002401448700003701472856013101509 2013 eng d a0039-128X00aBiocatalyst mediated production of 6 beta,11 alpha-dihydroxy derivatives of 4-ene-3-one steroids0 aBiocatalyst mediated production of 6 beta11 alphadihydroxy deriv a360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAbELSEVIER SCIENCE INCcNOV a1152-11580 v783 a

Biotransformation of steroids with 4-ene-3-one functionality such as progesterone (I), testosterone (II), 17 alpha-methyltestosterone (III), 4-androstene-3,17-dione (IV) and 19-nortestosterone (V) were studied by using a fungal system belonging to the genera of Mucor (M881). The fungal system efficiently and quantitatively converted these steroids in regio- and stereo-selective manner into corresponding 6 beta,11 alpha-dihydroxy compounds. Time course experiments suggested that the transformation was initiated by hydroxylation at 6 beta- or 11 alpha-(10 beta-hydroxy in case of V) to form monohydroxy derivatives which upon prolonged incubation were converted into corresponding 613,11oc-dihydroxy derivatives. The fermentation studies carried out using 5 L table-top fermentor with substrates (I and II) clearly indicates that 6 beta,11 alpha-dihydroxy derivatives of steroids with 4-ene-3-one functionality can be produced in large scale by using M881. (C) 2013 Elsevier Ltd.

10a4-Ene-3-one steroids10aBiotransformation10aMucor sp.1 aKolet, Swati, P.1 aNiloferjahan, Siddiqui1 aHaldar, Saikat1 aGonnade, Rajesh, G.1 aThulasiram, Hirekodathakallu, V. uhttps://library.ncl.res.in/content/biocatalyst-mediated-production-6-beta11-alpha-dihydroxy-derivatives-4-ene-3-one-steroids-001978nas a2200229 4500008004100000022001400041245011200055210006900167260007300236300001200309490000700321520111200328653002201440653002501462653003001487653000901517653001401526100002301540700003201563700002701595856012601622 2013 eng d a0141-813000aBiochemical characterization of recombinant cinnamoyl CoA reductase 1 (Ll-CCRH1) from Leucaena leucocephala0 aBiochemical characterization of recombinant cinnamoyl CoA reduct aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUL a154-1590 v583 a

Recombinant cinnamoyl CoA reductase 1 (Ll-CCRH1) protein from Leucaena leucocephala was overexpressed in Escherichia coli BL21 (DE3) strain and purified to apparent homogeneity. Optimum pH for forward and reverse reaction was found to be 6.5 and 7.8 respectively. The enzyme was most stable around pH 6.5 at 25 degrees C for 90 min. The enzyme showed k(cat)/k(m) for feruloyl, caffeoyl, sinapoyl, coumaroyl CoA, coniferaldehyde and sinapaldehyde as 4.6, 2.4, 2.3, 1.7, 1.9 and 1.2 (x10(6) M-1 s(-1)), respectively, indicating affinity of enzyme for feruloyl CoA over other substrates and preference of reduction reaction over oxidation. Activation energy, E-a for various substrates was found to be in the range of 20-50 kJ/mol. Involvement of probable carboxylate ion, histidine, lysine or tyrosine at the active site of enzyme was predicted by pH activity profile. SAXS studies of protein showed radius 3.04 nm and volume 49.25 nm(3) with oblate ellipsoid shape. Finally, metal ion inhibition studies revealed that Ll-CCRH1 is a metal independent enzyme. (C) 2013 Elsevier B.V. All rights reserved.

10aActivation energy10aCinnamoyl CoA esters10aCinnamoyl CoA reductase 110aSAXS10astability1 aSonawane, Prashant1 aVishwakarma, Rishi, Kishore1 aKhan, Bashir, Mohammad uhttps://library.ncl.res.in/content/biochemical-characterization-recombinant-cinnamoyl-coa-reductase-1-ll-ccrh1-leucaena-002552nas a2200289 4500008004100000022001400041245018000055210006900235260007400304300001000378490000800388520141500396653002101811653002801832653004001860653002101900653003201921653002401953653003801977100001802015700001902033700002002052700001702072700002102089700002602110856012602136 2013 eng d a0003-607200aBiochemical correlate of dimorphism in a zygomycete benjaminiella poitrasii: characterization of purified NAD-dependent glutamate dehydrogenase, a target for antifungal agents0 aBiochemical correlate of dimorphism in a zygomycete benjaminiell aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJUL a25-360 v1043 a

The fungal organisms, especially pathogens, change their vegetative (Y, unicellular yeast and H, hypha) morphology reversibly for survival and proliferation in the host environment. NAD-dependent glutamate dehydrogenase (NAD-GDH, EC 1.4.1.2) from a non-pathogenic dimorphic zygomycete Benjaminiella poitrasii was previously reported to be an important biochemical correlate of the transition process. The enzyme was purified to homogeneity and characterized. It is a 371 kDa native molecular weight protein made up of four identical subunits. Kinetic studies showed that unlike other NAD-GDHs, it may act as an anabolic enzyme and has more affinity towards 2-oxoglutarate than l-glutamate. Chemical modifications revealed the involvement of single histidine and lysine residues in the catalytic activity of the enzyme. The phosphorylation and dephosphorylation study showed that the NAD-GDH is present in active phosphorylated form in hyphal cells of B. poitrasii. Two of the 1,2,3 triazole linked beta-lactam-bile acid conjugates synthesized in the laboratory (B18, B20) were found to be potent inhibitors of purified NAD-GDH which also significantly affected Y-H transition in B. poitrasii. Furthermore, the compound B20 inhibited germ tube formation during Y-H transition in Candida albicans strains and Yarrowia lipolytica. The possible use of NAD-GDH as a target for antifungal agents is discussed.

10aAntifungal agent10aBenjaminiella poitrasii10aBiochemical correlate of dimorphism10aCandida albicans10aNAD-GDH activity regulation10aYarrowia lipolytica10aYeast-hypha reversible transition1 aJoshi, C., V.1 aPathan, E., K.1 aPunekar, N., S.1 aTupe, S., G.1 aKapadnis, B., P.1 aDeshpande, Mukund, V. uhttps://library.ncl.res.in/content/biochemical-correlate-dimorphism-zygomycete-benjaminiella-poitrasii-characterization-001373nas a2200229 4500008004100000022001400041245007800055210006900133260009900202300001000301490000700311520057900318653001900897653001300916653001800929653000900947653001400956653001900970100002500989700002001014856010901034 2013 eng d a0972-062600aBiodegradation of chlorobenzene and chlorophenols by pseudomonas cultures0 aBiodegradation of chlorobenzene and chlorophenols by pseudomonas aSECTOR A/80 SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE MP, 452 010, INDIAbDR JYOTI GARGcJUL a40-430 v173 a

Cultures from NCIM culture collection (National Chemical Laboratory, Pune, India) were screened for biodegradation chlorobenzene (CB). A strain, Pseudomonas sp. NCIM 2303, was found to be most effective. The biodegradation was carried out in aqueous solution by resting cells. The decomposition of 0.02% CB was 96.64% in 24h. We have screened cultures for biodegradation of o-chlorophenol and p-chlorophenol. Pseudomonas sp. NCIM 2668 has shown 94.59% degradation of 0.15% o-chlorophenol. Pseudomonas sp. NCIM 2668 has shown 31.42% degradation of 0.2% p-chlorophenol.

10aBiodegradation10achloride10achlorobenzene10aetc.10apollution10aPseudomonas sp1 aBhaskar, Gaikwad, G.1 aRita, Varma, J. uhttps://library.ncl.res.in/content/biodegradation-chlorobenzene-and-chlorophenols-pseudomonas-cultures-001863nas a2200265 4500008004100000022001400041245009900055210006900154260007900223300001400302490000800316520096200324653001701286653002001303653001401323653001101337653002101348653001101369100002101380700002401401700002001425700001801445700001501463856011901478 2013 eng d a0273-228900aBiodegradation of phenol by the yeast candida tropicalis: an investigation by NMR spectroscopy0 aBiodegradation of phenol by the yeast candida tropicalis an inve a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcAPR a2029-20370 v1693 a

The process of phenol biodegradation by the yeast Candida tropicalis NCIM 3556 in aqueous medium was studied by H-1, C-13, and DOSY NMR techniques. Samples at regular intervals were centrifuged to separate the cells, and H-1 spectral data were collected at 400 MHz. Though a gradual decrease in the concentration of phenol was observed, after an incubation period of similar to 8 h, formation of any intermediate products could not be detected. Experiments carried out with uniformly C-13-labeled phenol also failed to detect formation of any carboxylic acid intermediates during degradation. The studies indicated that the phenol was completely degraded to carbon dioxide and water in approximately 20 h. Self-diffusion coefficient measurements showed that the lifetime of phenol in the bound form is too small to impart any change in its diffusion behavior and the intermediates formed are converted to carbon dioxide and water at a very fast rate.

10abiocatalysis10aCarboxylic acid10aDiffusion10aEnzyme10aNMR spectroscopy10aphenol1 aPhalgune, U., D.1 aRajamohanan, P., R.1 aGaikwad, B., G.1 aVarma, R., J.1 aGeorge, S. uhttps://library.ncl.res.in/content/biodegradation-phenol-yeast-candida-tropicalis-investigation-nmr-spectroscopy-002065nas a2200241 4500008004100000022001400041245014800055210006900203260010600272300001200378490000800390520114400398653001901542653001101561653001701572653001801589653001101607100001501618700002301633700002101656700001701677856012901694 2013 eng d a1386-142500aBiological synthesis of silver nanoparticles using the fungus humicola sp and evaluation of their cytoxicity using normal and cancer cell lines0 aBiological synthesis of silver nanoparticles using the fungus hu aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcOCT a144-1470 v1143 a

Nanoscience is a new born science of the modern era and taps into the potential of particles at nanoscale. Bulk materials reduced to nanoscale dimensions thus obtain unique properties such as electronic, optical, magnetic and chemical. As far as synthesis of nanoparticles is concerned, biological synthesis has recently sparked a great interest as compared to other available chemical and physical methods on account of its eco-friendliness and cost-effectiveness. Here we report, for the first time, the biosynthesis of silver nanoparticles by the thermophilic fungus Humicola sp. The fungus when reacted with Ag+ ions reduces the precursor solution and leads to the formation of extracellular nanoparticles as monitored by ultra violet visible spectroscopy (UV-Vis). The morphology of nanoparticles is found to be spherical with good dispersity as revealed by transmission electron microscopy (TEM). Cell viability assays were carried out to assess the cytotoxicity of silver nanoparticles on NIH3T3 mouse embryonic fibroblast cell line and MDA-MB-231 human breast carcinoma cell line. (C) 2013 Elsevier B.V. All rights reserved.

10aCell viability10aFungus10aHumicola sp.10aNanoparticles10aSilver1 aSyed, Asad1 aSaraswati, Supriya1 aKundu, Gopal, C.1 aAhmad, Absar uhttps://library.ncl.res.in/content/biological-synthesis-silver-nanoparticles-using-fungus-humicola-sp-and-evaluation-their-001328nas a2200217 4500008004100000022001400041245010100055210006900156260007500225300001400300490000700314520048900321653003500810653001300845653003600858653002900894653001400923100002300937700001900960856013100979 2013 eng d a0039-788100aBiology-orientated synthesis of putrescine bisamides gigantamide A, dasyclamide, and cucullamide0 aBiologyorientated synthesis of putrescine bisamides gigantamide  aRUDIGERSTR 14, D-70469 STUTTGART, GERMANYbGEORG THIEME VERLAG KGcOCT a2888-28920 v453 a

Starting from putrescine and the requisite carboxylic acids, first syntheses of bioactive natural products gigantamide A, dasyclamide, and cucullamide were accomplished in very good overall yields using an appropriate sequence of dehydrative coupling reactions. The syntheses of the corresponding dehomo analogues of these natural products are also described. Regioselective diisobutylaluminum hydride reduction of an unhindered carbonyl group in citraconimide is the key step.

10adehydrative coupling reactions10adiamines10anatural and unnatural bisamides10aregioselective reduction10asynthesis1 aBatwal, Ramesh, U.1 aArgade, N., P. uhttps://library.ncl.res.in/content/biology-orientated-synthesis-putrescine-bisamides-gigantamide-dasyclamide-and-cucullamide-001287nas a2200157 4500008004100000022001400041245004300055210004100098260011000139300001600249490000600265520073700271100002001008700002601028856007501054 2013 eng d a2046-206900aBiomass to biodegradable polymer (PLA)0 aBiomass to biodegradable polymer PLA aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJUN a13558-135680 v33 a

Lignocellulosic biomass is renewable and cheap, and it has the potential to displace fossil fuels for the production of fuels and chemicals. Biomass derived lactic acid is an important compound that can be used as a chemical platform for the production of a variety of important chemicals on a large scale. The quality of the monomers of lactic acid and lactide, as well as the chemical changes induced during polymerization and processing, are crucial parameters for controlling the properties of the resulting polylactic acid (PLA) products. In this review, we outline the process of exploiting biomass for the production of polylactic acid, a biodegradable polymer which is well-known as a sustainable bioplastic material.

1 aSinghvi, Mamata1 aGokhale, Digambar, V. uhttps://library.ncl.res.in/content/biomass-biodegradable-polymer-pla-001829nas a2200145 4500008004100000022001400041245009200055210006900147260010400216300001100320520118700331100002601518700002101544856011801565 2013 eng d a2314-613300aBiosurfactant-sophorolipid acts in synergy with antibiotics to enhance their efficiency0 aBiosurfactantsophorolipid acts in synergy with antibiotics to en a410 PARK AVENUE, 15TH FLOOR, \#287 PMB, NEW YORK, NY 10022 USAbHINDAWI PUBLISHING CORPORATIONcAPR a5124953 a

Sophorolipids (SLs), biosurfactants with antimicrobial properties, have been tried to address the problem of antibiotic resistance. The synergistic action of SL and antibiotics was checked using standard microdilution and spread plate methods. With Staphylococcus aureus, SL-tetracycline combination achieved total inhibition before 4 h of exposure while tetracycline alone couldnot achieve total inhibition till the end of 6 h. The inhibition caused by exposure of bacterium to SL-tetracycline mixture was similar to 25% more as compared to SL alone. In spite of known robustness of gram-negative bacteria, SL-cefaclor mixture proved to be efficient against Escherichia coli which showed similar to 48% more inhibition within 2 h of exposure as compared to cefaclor alone. Scanning electron microscopy of the cells treated with mixture revealed bacterial cell membrane damage and pore formation. Moreover, SLs being a type of asymmetric bola, they are expected to form self-assemblies with unique functionality. This led to the speculation that SLs being amphiphilic in nature can span through the structurally alike cell membrane and facilitate the entry of drug molecules.

1 aJoshi-Navare, Kasturi1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/biosurfactant-sophorolipid-acts-synergy-antibiotics-enhance-their-efficiency-001988nas a2200349 4500008004100000022001400041245009800055210006900153260008900222300001200311490000800323520087500331100001401206700001501220700001601235700002001251700001501271700002401286700002001310700001401330700002101344700001601365700001601381700001301397700001601410700001401426700001701440700001901457700002001476700001901496856012301515 2013 eng d a0036-807500aBiosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes0 aBiosynthesis of antinutritional alkaloids in solanaceous crops i a1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USAbAMER ASSOC ADVANCEMENT SCIENCEcJUL a175-1790 v3413 a

Steroidal glycoalkaloids (SGAs) such as alpha-solanine found in solanaceous food plants-as, for example, potato-are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

1 aItkin, M.1 aHeinig, U.1 aTzfadia, O.1 aBhide, Amey, J.1 aShinde, B.1 aCardenas, Pablo, D.1 aBocobza, S., E.1 aUnger, T.1 aMalitsky, Sergey1 aFinkers, R.1 aTikunov, Y.1 aBovy, A.1 aChikate, Y.1 aSingh, P.1 aRogachev, I.1 aBeekwilder, J.1 aGiri, Ashok, P.1 aAharoni, Asaph uhttps://library.ncl.res.in/content/biosynthesis-antinutritional-alkaloids-solanaceous-crops-mediated-clustered-genes-002522nas a2200265 4500008004100000022001400041245007700055210006900132260007300201300001200274490000800286520164000294653001601934653000901950653001001959653001401969653002701983653002902010100002802039700001802067700001802085700001802103700002702121856010802148 2013 eng d a0927-776500aBiosynthesis of Se nanoparticles and its effect on UV-induced DNA damage0 aBiosynthesis of Se nanoparticles and its effect on UVinduced DNA aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR a261-2660 v1033 a

This paper reports, an environmentally benign procedure of synthesis and characterizations of selenium nanoparticles and their protective effect against UV-induced DNA damage activities. An aqueous leaf extract of lemon plant was used as a precursor for synthesis of colloidal selenium nanoparticles. Resulting nanoparticles were characterized using UV-vis spectrophotometer, photoluminescence, TEM, EDAX, FT-IR and XRD, respectively. Selenium colloidal solution exhibited an absorption maximum at 395 nm and produced an emission maximum at 525 nm. Transmission electron microscopy followed by selected area electron diffraction pattern analysis indicated the formation of spherical, polydispersed, crystalline, selenium nanoparticles of diameter ranging from (similar to 60 to 80 nm). X-ray diffraction studies showed the formation of 111, 2 0 0 and 220 planes of face-centered cubic (fcc) selenium. EDAX analysis confirmed the presence of selenium in nanosphere. Fourier transformed infrared spectroscopic investigation reveled the involvement of carboxyl (-C=0), hydroxyl (-OH), amine (-NH) functional group of lemon plant extract in preparation of selenium nano particles. MU assay as well single cell gel electrophoresis assay or comet assay revealed that synthesized selenium nanoparticles, caused less cell death of lymphocytes and prevented DNA damage, when cells were exposed to UVB. The fluorescent property of selenium nanopartides can be used as diagnostic agent. Further, their anti DNA damaging property can be investigated as a chemotherapeutic agent in cancer therapy. (C) 2012 Elsevier B.V. All rights reserved.

10aComet assay10aEDAX10aFT-IR10aMTT assay10aSelenium nanoparticles10aUV-vis spectrophotometer1 aPrasad, Kumar, Suranjit1 aPatel, Hirnee1 aPatel, Tirtha1 aPatel, Khusbu1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/biosynthesis-se-nanoparticles-and-its-effect-uv-induced-dna-damage-001875nas a2200217 4500008004100000022001400041245013000055210006900185260009000254300001000344490000700354520103800361653002201399653001501421653002301436653001601459653001601475100002001491700001701511856012901528 2013 eng d a1024-242200aBiotransformation of vinblastine to vincristine by the endophytic fungus fusarium oxysporum isolated from catharanthus roseus0 aBiotransformation of vinblastine to vincristine by the endophyti aTELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLANDbINFORMA HEALTHCAREcAPR a89-930 v313 a

A fungal transformation method for the production of the less abundant but more valuable anticancer drug vincristine using the more abundant anticancer drug vinblastine by an endophytic fungus Fusarium oxysporum isolated from the plant Catharanthus roseus is described. Vinblastine dissolved in sterile water when incubated with the fungal mycelial mass was transformed into vincristine at room temperature. The biotransformation process was monitored by periodic sampling of aliquots that were extracted with ethyl acetate. The crude transformed compounds were analysed using TLC where the Rf value of the spot obtained on the 8th day was found to be similar to that of standard vincristine (Rf 0.74). Purification by HPLC using a C 18 symmetry column showed a peak with RT (34.9 min) similar to that of standard vincristine. Further characterization with the help of ESI-MS showed a peak at m/z 825 attributed to the (M + H)(+) ion. This indicated that the fungus Fusarium oxysporum had converted vinblastine to vincristine.

10aBiotransformation10aendophytes10afusarium oxysporum10aVinblastine10avincristine1 aKumar, Ashutosh1 aAhmad, Absar uhttps://library.ncl.res.in/content/biotransformation-vinblastine-vincristine-endophytic-fungus-fusarium-oxysporum-isolated-001669nas a2200253 4500008004100000022001400041245009500055210006900150260007300219300001200292490000800304520069300312653004101005653003501046653003001081653003701111653002201148653003701170100001801207700001801225700001801243700002501261856012901286 2013 eng d a0169-433200aBulk and surface structure characterization of nanoscopic silver doped lanthanum chromites0 aBulk and surface structure characterization of nanoscopic silver aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN a574-5800 v2643 a

Crystalline state of lanthanum chromites with silver as a dopant has been studied by X-ray diffraction and transmission electron microscopy reveals microscopic properties of grain boundaries. X-ray photoelectron spectroscopy has been used to analyze surface states with atomic ratio of La, Cr, O and Ag as a dopant. LaCrO3 shows mixed valence states of chromium while the silver doped samples exhibit differences in chromium concentration with the oxidation of Cr3+ to Cr6+ in presence of chromium nitrate as a precursor salt. Trivalent stable state of chromium is observed for samples synthesized by chromic acetate as a precursor salt. (C) 2012 Elsevier B.V. All rights reserved.

10aEnergy dispersive analysis of X-rays10aMicrowave combustion synthesis10aPerovskite nanostructures10atransmission electron microscopy10aX-ray diffraction10aX-ray photoelectron spectroscopy1 aDesai, P., A.1 aJoshi, P., N.1 aPatil, K., R.1 aAthawale, Anjali, A. uhttps://library.ncl.res.in/content/bulk-and-surface-structure-characterization-nanoscopic-silver-doped-lanthanum-chromites-002102nas a2200241 4500008004100000022001400041245006200055210006000117260007400177300001200251490000800263520130200271653001601573653002001589653001501609100002601624700002501650700001901675700003001694700002001724700002401744856009201768 2014 eng d a0003-607200aBacillus enclensis sp nov., isolated from sediment sample0 aBacillus enclensis sp nov isolated from sediment sample aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJAN a199-2060 v1053 a

A novel bacterial strain, designated SGD-1123(T) was isolated from Chorao Island, in Goa Province, India. The strain was found to be able to grow at 15-42 A degrees C, pH 5-12 and 0-12 % (w/v) NaCl. The whole cell hydrolysates were found to contain meso-diaminopimelic acid, galactose and arabinose. The major fatty acids were identified as iso-C-15:0 and anteiso-C-15:0, MK-7 was identified as the predominant menaquinone and the predominant polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The genomic DNA G+C content was determined to be 44.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and further revealed that strain SGD-1123(T) had highest sequence similarity with Bacillus aquimaris, and forms a separate clade with its closest relatives i.e. B. aquimaris, Bacillus vietnamensis and Bacillus marisflavi, with which it shares 94.5, 94.1 and 94.1 % similarity respectively. The phylogenetic, chemotaxonomic and phenotypic analyses indicated that strain SGD-1123(T) represents a novel species within the genus Bacillus, for which the name Bacillus enclensis is proposed. The type strain is SGD-1123(T) (NCIM 5450(T)=CCTCC AB 2011125(T)).

10aBacillus sp10aMarine sediment10aPolyphasic1 aDastager, Syed, Gulam1 aMawlankar, Rahul, R.1 aTang, Shan-Kun1 aSrinivasan, Krishnamurthi1 aRamana, Venkata1 aShouche, Yogesh, S. uhttps://library.ncl.res.in/content/bacillus-enclensis-sp-nov-isolated-sediment-sample-001165nas a2200181 4500008004100000022001400041245017800055210007900233260000800312300001400320490000600334520040500340100001900745700002000764700001700784700001400801856016800815 2014 eng d a2040-337200aBack-to-back MOS–Schottky (Pt–SiO2–Si–C–Pt) nano-heterojunction device as an efficient self-powered photodetector: one step fabrication by pulsed laser deposition 0 aBacktoback MOS–Schottky Pt–SiO2–Si–C–Pt nanoheterojunction devic cJAN a3550-35560 v63 aAn efficient self-powered photodetector design involving a C–Si hetero-interface with back-to-back MOS–Schottky (Pt–SiO2–Si–C–Pt) device action is presented. Pulsed laser deposition of a carbon thin film is used which dynamically removes the native surface oxide to form the desired Schottky interface. The combined device action yields two orders of magnitude photoresponse at zero bias.1 aShaikh, P., A.1 aThakare, V., P.1 aLate, D., J.1 aOgale, S. uhttps://library.ncl.res.in/content/back-back-mos%E2%80%93schottky-pt%E2%80%93sio2%E2%80%93si%E2%80%93c%E2%80%93pt-nano-heterojunction-device-efficient-self-powered01661nas a2200157 4500008004100000022001400041245017500055210006900230260007100299300001400370490000700384520094400391100002401335700001901359856012501378 2014 eng d a0022-326300aBase-stimulated 1,2-, 1,4-, and 1,6-eliminations in suitably substituted alkylidenesuccinates leading to natural and unnatural conjugated alkenyl(methyl)maleic anhydrides0 aBasestimulated 12 14 and 16eliminations in suitably substituted  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcMAR a2538-25460 v793 a

With dimethyl maleate as the starting material, facile stereoselective syntheses of natural and unnatural conjugated alkenyl(methyl)maleic anhydrides have been described. The key reactions were base-endorsed novel 1,2-, 1,4-, and 1,6-eliminations in the corresponding alkylidenesuccinate derivatives. The 1,2-eliminations in cyclic carbonate and sulfite by regioselective abstraction of methine protons with the respective release of CO2 and SO2 provided a conjugated ketone product. The characteristic 1,4- and 1,6-elimination reactions with respective release of acetone and mesylate furnished the corresponding unsaturated alcohols. The obtained allylic alcohols were transformed into conjugated alkenyl(methyl)maleic anhydrides via oxidation followed by a Horner-Wadsworth-Emmons reaction pathway in very good yields. The mechanistic aspects involved in these significant elimination reactions have also been described in brief.

1 aDeore, Prashant, S.1 aArgade, N., P. uhttps://library.ncl.res.in/content/base-stimulated-12-14-and-16-eliminations-suitably-substituted-alkylidenesuccinates-001282nas a2200205 4500008004100000022001400041245008100055210006900136260011000205300001600315490000600331520047900337100001900816700002100835700002700856700002100883700002400904700003700928856011100965 2014 eng d a2046-206900aBiocatalyst mediated functionalization of salannin, an insecticidal limonoid0 aBiocatalyst mediated functionalization of salannin an insecticid aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJUN a27661-276640 v43 a

Transformation of salannin, an insecticidal C-seco limonoid was investigated using a fungal system, Cunninghamella echinulata. Salannin was efficiently converted into two metabolites, where the C-17 furan moiety was transformed into gamma-hydroxybutenolide (salanninolide) and N-(2-hydroxyethyl)-alpha,beta-unsaturated-gamma-lactam (salanninactam) analogues. Present studies have indicated salanninolide to be a metabolite in the C-seco limonoid biosynthetic pathway.

1 aHaldar, Saikat1 aKolet, Swati, P.1 aDandekar, Devdutta, S.1 aKale, Balaji, S.1 aGonnade, Rajesh, G.1 aThulasiram, Hirekodathakallu, V. uhttps://library.ncl.res.in/content/biocatalyst-mediated-functionalization-salannin-insecticidal-limonoid-001309nas a2200181 4500008004100000022001400041245010500055210006900160260011000229300001400339490000700353520052600360100002500886700003100911700002100942700003700963856012701000 2014 eng d a1477-052000aBiocatalyst mediated regio- and stereo-selective hydroxylation and epoxidation of (Z)-alpha-santalol0 aBiocatalyst mediated regio and stereoselective hydroxylation and aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJAN a1048-10510 v123 a

Biocatalyst mediated regio- and stereo-selective hydroxylation and epoxidation on (Z)-alpha-santalol were achieved for the first time, using a fungal strain Mucor piriformis. Four novel metabolites were characterized as 10,11-cis-beta-epoxy-alpha-santalol, 5 alpha-hydroxy-(Z)-alpha-santalol, 10,11-dihydroxy-alpha-santalol and 5 alpha-hydroxy-10,11-cis-beta-epoxy-alpha-santalol. Using Amano PS lipase from Burkholderia cepacia, alpha- and beta-isomers of 10,11-cis-epoxy-alpha-santalol were resolved efficiently.

1 aDaramwar, Pankaj, P.1 aSrivastava, Prabhakar, Lal1 aKolet, Swati, P.1 aThulasiram, Hirekodathakallu, V. uhttps://library.ncl.res.in/content/biocatalyst-mediated-regio-and-stereo-selective-hydroxylation-and-epoxidation-z-alpha-002522nas a2200265 4500008004100000022001400041245011700055210006900172260009500241300001400336490000700350520150500357653003001862653003101892653001801923653002301941653002501964100001801989700002902007700002202036700002402058700002302082700002602105856012502131 2014 eng d a0263-876200aBiodegradable iron chelate for H2S abatement: modeling and optimization using artificial intelligence strategies0 aBiodegradable iron chelate for H2S abatement modeling and optimi a165-189 RAILWAY TERRACE, DAVIS BLDG, RUGBY CV21 3HQ, ENGLANDbINST CHEMICAL ENGINEERScJUN a1119-11320 v923 a

A batch reactor process for the abatement of a common pollutant, namely, H2S using Fe3+-malic acid chelate (Fe3+-MA) catalyst has been developed. Further, process modeling and optimization was conducted in the three stages with a view to maximize the H2S conversion: (i) sensitivity analysis of process inputs was performed to select the most influential process operating variables and parameters, (ii) an artificial neural network (ANN)-based data-driven process model was developed using the influential process variables and parameters as model inputs, and H2S conversion (%) as the model output, and (iii) the input space of the ANN model was optimized using the artificial immune systems (AIS) formalism. The AIS is a recently proposed stochastic nonlinear search and optimization method based on the human biological immune system and has been introduced in this study for chemical process optimization. The AIS-based optimum process conditions have been compared with those obtained using the genetic algorithms (GA) formalism. The AIS-optimized process conditions leading to high (approximate to 97%) H2S conversion, were tested experimentally and the results obtained thereby show an excellent match with the AIS-maximized H2S conversion. It was also observed that the AIS required lesser number of generations and function evaluations to reach the convergence when compared with the GA. (C) 2013 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

10aArtificial immune systems10aArtificial neural networks10aBatch reactor10aGenetic algorithms10aSensitivity analysis1 aHamid, Aashti1 aDeshpande, Aniruddha, S.1 aBadhe, Yogesh, P.1 aBarve, Prashant, P.1 aTambe, Sanjeev, S.1 aKulkarni, Bhaskar, D. uhttps://library.ncl.res.in/content/biodegradable-iron-chelate-h2s-abatement-modeling-and-optimization-using-artificial-001662nas a2200229 4500008004100000022001400041245011500055210006900170260007900239300001200318490000800330520080900338653001301147653001601160653000901176653001401185653002701199653002201226100002801248700002701276856012901303 2014 eng d a0163-498400aBiogenic synthesis of selenium nanoparticles and their effect on as(III)-induced toxicity on human lymphocytes0 aBiogenic synthesis of selenium nanoparticles and their effect on a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcMAR a275-2830 v1573 a

{A bioreductive capacity of a plant, Terminalia arjuna leaf extract, was utilized for preparation of selenium nanoparticles. The leaf extract worked as good capping as well as stabilizing agent and facilitated the formation of stable colloidal nanoparticles. Resulting nanoparticles were characterized using UV-Vis spectrophotometer, transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDAX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction analysis (XRD), respectively. The colloidal solution showed the absorption maximum at 390 nm while TEM and selected area electron diffraction (SAED) indicated the formation of polydispersed, crystalline selenium nanoparticles of size raging from 10 to 80 nm. FT-IR analysis suggested the involvement of O-H, N-H

10aArsenite10aComet assay10aEDAX10aMTT assay10aSelenium nanoparticles10aTerminalia arjuna1 aPrasad, Kumar, Suranjit1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/biogenic-synthesis-selenium-nanoparticles-and-their-effect-asiii-induced-toxicity-human-001717nas a2200265 4500008004100000022001400041245008300055210006900138260007300207300001200280490000800292520084600300653001701146653001301163653001801176653001601194653001401210653000801224100001301232700002701245700002701272700002101299700001701320856011401337 2014 eng d a0167-577X00aBiological synthesis of cationic gold nanoparticles and binding of plasmid DNA0 aBiological synthesis of cationic gold nanoparticles and binding  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcAUG a159-1610 v1293 a

Nanobiotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials. The present work focuses on synthesis of cationic gold nanoparticles (C-GNPs) for biological applications, especially in gene and drug delivery studies. A biosynthesis methodology has been developed for the functionalization of gold nanoparticles to cationic nature. The synthesis of C-GNPs was done by using peanut leaf extract in the presence of cysteamine. The formed C-GNPs were characterized by using UV-visible spectroscopy (UV-vis), the particles sizes and shapes were confirmed by a Transmission electron microscope (TEM) and crystallinity of C-GNPs was characterized by diffraction. The binding of plasmid DNA on the C-GNPs was confirmed by agarose gel electrophoresis. (C) 2014 Elsevier B.V. All rights reserved.

10aBiomaterials10aCationic10aNanoparticles10aPlasmid DNA10asynthesis10aTEM1 aRaju, D.1 aVishwakarma, Rishi, K.1 aKhan, Bashir, Mohammad1 aMehta, Urmil, J.1 aAhmad, Absar uhttps://library.ncl.res.in/content/biological-synthesis-cationic-gold-nanoparticles-and-binding-plasmid-dna-001704nas a2200157 4500008004100000022001400041245012100055210006900176260007100245300001400316490000800330520103800338100002601376700001701402856012701419 2014 eng d a1520-610600aBlue, green, and orange-red emission from Polystyrene microbeads for solid-state white-light and multicolor emission0 aBlue green and orangered emission from Polystyrene microbeads fo a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcAUG a9467-94750 v1183 a

Solid-state white-light emission was achieved from polystyrene (PS) microbeads incorporated with fluorophores based on perylene bisimide (PBITEG) and oligo(p-phenylenevinylene) (OPV) as acrylic cross-linkers. The PS beads incorporated with only PBITEG gave intense orange-red emission; PS incorporated with OPV exhibited blue-emission, whereas a series of polymers incorporating both cross-linkers exhibited varying shades of white-light emission. One of the PS samples, PS-PBITEG-6.25-OPV-4.28 (PBITEG incorporation: 6.25 X 10(-7) mole; OPV incorporation: 4.28 x 10(-7) mol), exhibited pure white-light emission in the powder form with CIE coordinates (0.33, 0.32). The rigid aromatic cross-linkers were incorporated into the PS backbone in a two-stage dispersion polymerization to afford PS beads in the size range 2 to 3 mu m. The incorporation of fluorophores as cross-linkers enabled covalent attachment of the dye to the polymer backbone, avoiding dye leakage besides avoiding aggregation-induced fluorescence quenching.

1 aSonawane, Swapnil, L.1 aAsha, S., K. uhttps://library.ncl.res.in/content/blue-green-and-orange-red-emission-polystyrene-microbeads-solid-state-white-light-and-001262nas a2200217 4500008004100000022001400041245014200055210006900197260007700266300001200343490000700355520041700362653001500779653001800794653002600812653002800838653001300866100001900879700001700898856012900915 2014 eng d a0934-086600aBlue luminescent graphene quantum dots by photochemical stitching of small aromatic molecules: fluorescent nanoprobes in cellular imaging0 aBlue luminescent graphene quantum dots by photochemical stitchin aBOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcAPR a433-4380 v313 a

Blue fluorescent graphene quantum dots (GQDs) are synthesized from small haloaromatic molecules by laser photochemistry. The process involves a bottom-up photochemical stitching mechanism of the free radicals generated by irradiation of ultraviolet photons (lambda = 248 nm) on o-dichlorobenzene. The GQDs are further demonstrated to be of importance as fluorescent nanoprobes in bioimaging of cells.image

10abioimaging10afree radicals10agraphene quantum dots10aphotochemical stitching10aUV laser1 aGokhale, Rohan1 aSingh, Pooja uhttps://library.ncl.res.in/content/blue-luminescent-graphene-quantum-dots-photochemical-stitching-small-aromatic-molecules-002272nas a2200217 4500008004100000022001400041245006100055210006100116260011800177300001400295490000700309520148700316100002601803700002501829700001701854700001801871700003001889700001801919700002401937856009301961 2015 eng d a1466-502600aBacillus encimensis sp nov isolated from marine sediment0 aBacillus encimensis sp nov isolated from marine sediment aMARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLANDbSOC GENERAL MICROBIOLOGYcMAY a1421-14250 v653 a

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium designated SGD-V-25(T) was isolated from Veraval sediment sample, India. Strain SGD-V-25(T) was capable of growing at 25-50 degrees C (optimum 37 degrees C), pH 6-12 (optimum pH 7.0) and with 0-5 % (w/v) NaCl. The taxonomic position of this strain was deduced using a polyphasic approach and the 16S rRNA gene sequence analysis showed that the isolate belongs to the phylum Firmicutes, forming the cluster with Bacillus badius MTCC 1548(T), with which it shares highest similarity of 99.1 % with 13 nt differences. Other type strains of the genus Bacillus showed less than 96% similarity. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipid profile of strain SGD-V-25(T) showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phsophoglycolipid and two aminophospholipids. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were iso-C-15:0, anteiso-C-15:0, anteiso-C-17:0 iso-C-16:0, C-16:1 omega 11c and C-16:0. The genomic DNA G + C content of strain SGD-V-25(T) was 37.6 molok. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA hybridization, strain SGD-V-25(T) could be clearly distinguished from closely related members of the genus Bacillus, and the name Bacillus encimensis sp. nov., is proposed to accommodate this strain. The type strain is SGD-V-25(T) (= NCIM 5513(T)=DSM 28241(T)).

1 aDastager, Syed, Gulam1 aMawlankar, Rahul, R.1 aMual, Poonam1 aVerma, Ashish1 aKrishnamurthi, Srinivasan1 aJoseph, Neeta1 aShouche, Yogesh, S. uhttps://library.ncl.res.in/content/bacillus-encimensis-sp-nov-isolated-marine-sediment-002460nas a2200253 4500008004100000022001400041245006500055210006300120260007400183300001200257490000800269520164200277653001301919653001401932653001501946653001301961100002401974700002501998700002002023700001802043700002402061700002602085856009502111 2015 eng d a0003-607200aBacillus filamentosus sp nov., isolated from sediment sample0 aBacillus filamentosus sp nov isolated from sediment sample aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB a433-4410 v1073 a

A novel Gram-stain positive, endospore-forming bacterium, designated SGD-14(T), was isolated from a marine sediment sample in Goa Province, India. Cells of the isolate were found to be strictly aerobic. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SGD-14(T) showed a similarity of 99.5 % with Bacillus endophyticus and similarities to other Bacillus type strains were below 96 %. The whole-cell sugar pattern was found to consist of ribose, xylose and glucose. The predominant menaquinone was identified as MK-7 and the major fatty acids as anteiso-C-15:0, iso-C-15:0, iso-C-16:0, anteiso-C-17:0, C-16:0 and iso-C-14:0. The strain was found to grow optimally at 30 A degrees C and pH 7.0-7.5. DNA G + C content was determined to be 39.6 mol%. The phospholipid pattern was found to consist of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. DNA-DNA hybridization studies between strain SGD-14(T) and B. endophyticus CIP106778(T) showed that strain SGD-14(T) exhibited < 70 % similarity with B. endophyticus CIP106778(T). Differences in phenotypic and chemotaxonomic characteristics between the novel strain and B. endophyticus CIP106778(T) further confirmed that this isolate represents novel species. Phylogenetic analysis showed that strain SGD-14(T) fits in the same clade with B. endophyticus with 100 % bootstrap values. Strain SGD-14(T) is therefore considered to represent a novel species of the genus Bacillus, for which the name Bacillus filamentosus sp. nov. is proposed. The type strain of Bacillus filamentosus is SGD-14(T) = (=NCIM 5491(T) = DSM 27955(T)).

10aBacillus10aFilaments10aPolyphasic10aSediment1 aSonalkar, Vidya, V.1 aMawlankar, Rahul, R.1 aRamana, Venkata1 aJoseph, Neeta1 aShouche, Yogesh, S.1 aDastager, Syed, Gulam uhttps://library.ncl.res.in/content/bacillus-filamentosus-sp-nov-isolated-sediment-sample-002188nas a2200241 4500008004100000022001400041245009900055210006900154260007300223300001200296490000700308520130000315653004101615653002401656653002601680653001401706100002101720700002701741700001701768700001601785700002701801856011801828 2015 eng d a0141-813000aBacopa monniera recombinant mevalonate diphosphate decarboxylase: biochemical characterization0 aBacopa monniera recombinant mevalonate diphosphate decarboxylase aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcAUG a661-6680 v793 a

Mevalonate diphosphate decarboxylase (MDD; EC 4.1.1.33) is an important enzyme in the mevalonic acid pathway catalyzing the Mg2+-ATP dependant decarboxylation of mevalonate 5-diphosphate (MVAPP) to isopentenyl diphosphate (IPP). Bacopa monniera recombinant MDD (BmMDD) protein was overexpressed in Escherichia coli BL21 (DE3) strain and purified to apparent homogeneity. K-m and V-max for MVAPP were 144 mu M and 52 U mg(-1) respectively. The values of turnover (k(cat)) and k(cat)/K-m, for mevalonate 5-diphosphate were determined to be 40 s(-1) and 2.77 x 10(5) M-1 s(-1) and k(cat) and k(cat)/K-m values for ATP were found to be 30 s(-1) and 2.20 x 10(4) M-1 s(-1), respectively. pH activity profile indicated the involvement of carboxylate ion, lysine and arginine for the activity of enzyme. The apparent activation energy for the BmMDD catalyzed reaction was 12.7 kJ mol(-1). Optimum pH and temperature for the forward reaction was found to be 8.0 and 45 degrees C. The enzyme was most stable at pH 7 at 20 degrees C with the deactivation rate constant (K-d(*)) of 1.69 x 10(-4) and half life (t(1/2)) of 68 h. The cation studies suggested that BmMDD is a cation dependant enzyme and optimum activity was achieved in the presence of Mg2+. (C) 2015 Elsevier B.V. All rights reserved.

10aMevalonate diphosphate decarboxylase10apH activity profile10aPhylogenetic analysis10astability1 aAbbassi, Shakeel1 aVishwakarma, Rishi, K.1 aPatel, Parth1 aKumari, Uma1 aKhan, Bashir, Mohammad uhttps://library.ncl.res.in/content/bacopa-monniera-recombinant-mevalonate-diphosphate-decarboxylase-biochemical-102167nas a2200253 4500008004100000022001400041245009900055210006900154260009500223300001200318490000700330520123800337653001601575653001901591653001901610653001601629653002201645653002201667100002901689700002401718700002001742700002201762856012901784 2015 eng d a0263-876200aBenzylation of acetic acid to benzyl acetate over highly active and reusable micro/meso-HZSM-50 aBenzylation of acetic acid to benzyl acetate over highly active  a165-189 RAILWAY TERRACE, DAVIS BLDG, RUGBY CV21 3HQ, ENGLANDbINST CHEMICAL ENGINEERScJAN a584-5900 v933 a

Catalytic benzylation of acetic acid (AA) with benzyl alcohol (BA) to benzyl acetate was studied over zeolites viz. H-ZSM-5 (microporous, medium pore), Micro/Meso-HZSM-5 (combination of micro- and meso pore), H-Beta (microporous, large pore) to investigate catalytic activity and selectivity towards benzyl acetate. Micro/Meso-HZSM-5 obtained by desilication post-treatment has been employed as a heterogeneous catalyst for benzylation reaction probably for the first time. Micro/Meso-HZSM-5 was found to be a promising catalyst for benzylation with AA conversion of 94%, selectivity towards benzyl acetate of 95%. The detailed optimization of process parameters such as molar ratio, catalyst loading, reaction temperature and time was also presented. Micro/Meso-HZSM-5 catalyst was observed to be stable for six cycles (1 fresh and 5 recycles). The first order reaction kinetics (R-2 >0.98) indicated that reaction rate constants increased with increasing reaction temperature. The activation energy for benzylation of AA with BA over Micro/Meso-HZSM-5 was obtained to be 15.07 kJ mol(-1), which is far less than the reported. (C) 2014 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

10aAcetic acid10aBenzyl acetate10aBenzyl alcohol10aBenzylation10aMicro/Meso-HZSM-510aReaction kinetics1 aNandiwale, Kakasaheb, Y.1 aGalande, Nitish, D.1 aRaut, Sunil, A.1 aBokade, Vijay, V. uhttps://library.ncl.res.in/content/benzylation-acetic-acid-benzyl-acetate-over-highly-active-and-reusable-micromeso-hzsm-5-001217nas a2200193 4500008004100000022001400041245013800055210006900193260011000262300001400372490000700386520040200393100001700795700002000812700001700832700001800849700002600867856013000893 2015 eng d a1359-734500aBeta, gamma-Bis-substituted PNA with configurational and conformational switch: preferred binding to cDNA/RNA and cell-uptake studies0 aBeta gammaBissubstituted PNA with configurational and conformati aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcMAR a7693-76960 v513 a

(S,S)- and (R,R)-beta,gamma-Bis-substituted PNAs were synthesized from the C-2 symmetric vicinal diamine system embedded in 1,4 dihydroxybutane and 1,4-dimethoxybutane scaffolds. (R,R)-beta,gamma-Bis-methoxymethyl-PNA derived from D-tartaric acid was found to be in the right configuration and conformation to be an excellent mimic of PNA, endowed with superior ability to enter into cells.

1 aBose, Tanaya1 aBanerjee, Anjan1 aNahar, Smita1 aMaiti, Souvik1 aKumar, Vaijayanti, A. uhttps://library.ncl.res.in/content/beta-gamma-bis-substituted-pna-configurational-and-conformational-switch-preferred-binding01833nas a2200157 4500008004100000022001400041245015200055210006900207260011000276300001200386490000700398520110000405100001901505700002501524856012601549 2015 eng d a1744-683X00aBeta self-association and adsorption on a hydrophobic nanosurface: competitive effects and the detection of small oligomers via electrical response0 aBeta selfassociation and adsorption on a hydrophobic nanosurface aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcOCT a269-2790 v113 a

Treatment of Alzheimer's disease (AD) is impeded by the lack of effective early diagnostic methods. Small, soluble A beta globulomers play a major role in AD neurotoxicity, and detecting their presence in aqueous fluids could lead to suitable sensors. We evaluate the adsorption behavior of small A beta oligomers on the surface of a single walled carbon nanotube of high curvature. While the intrinsic self-assembly propensity of A beta is markedly hindered by adsorption, the oligomeric units show high degrees of surface immobilization. Immobilized complexes are capable of oligomeric growth, but with a shifted monomer-oligomer equilibrium compared to the free states. In the presence of an ionic solution and suitable external electric fields, magnitudes of the current blockades are found to be sensitive to the oligomeric number of the adsorbed complex. However, this sensitivity gradually diminishes with increasing oligomeric size. The results provide a proof-of-concept basis for further investigations in the design of sensors for detecting the toxic small oligomers of A beta.

1 aJana, Asis, K.1 aSengupta, Neelanjana uhttps://library.ncl.res.in/content/beta-self-association-and-adsorption-hydrophobic-nanosurface-competitive-effects-and-001116nas a2200193 4500008004100000022001400041245009300055210006900148260011000217300001200327490000700339520033400346100002900680700002400709700002100733700002300754700002000777856012500797 2015 eng d a1359-734500aBifunctional covalent organic frameworks with two dimensional organocatalytic micropores0 aBifunctional covalent organic frameworks with two dimensional or aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcNOV a310-3130 v513 a

We report the successful incorporation of bifunctional (acid/base) catalytic sites in the crystalline organocatalytic porous COF (2,3-DhaTph). Due to the presence of acidic (catachol) and basic (porphyrin) sites, 2,3-DhaTph shows significant selectivity, reusability, and excellent ability to perform the cascade reaction.

1 aShinde, Digambar, Balaji1 aKandambeth, Sharath1 aPachfule, Pradip1 aKumar, Raya, Rahul1 aBanerjee, Rahul uhttps://library.ncl.res.in/content/bifunctional-covalent-organic-frameworks-two-dimensional-organocatalytic-micropores-001662nas a2200193 4500008004100000022001400041245008600055210006900141260011000210300001200320490000700332520089000339100002201229700002401251700002701275700002401302700002201326856012001348 2015 eng d a1144-054600aBile acid hydrazides: gelation, structural, physical and spectroscopic properties0 aBile acid hydrazides gelation structural physical and spectrosco aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJAN a453-4600 v393 a

Synthesis and gelation properties of a series of novel bile acid hydrazides are presented. These compounds are found to undergo self-assembly leading to organogelation in certain organic solvents. Compound 1 was found to be the most ``effective'' gelator in this series. The properties of this gel have been thoroughly investigated by conventional methods typical for molecular gel studies. Sol gel transition temperature (T-g) of chloroform gels of compounds 1 and 3 was found to increase with increase in the chain length. Sol-gel transition was probed using the isothermal time test and results show that there is instantaneous increase in both the moduli after shear melting, which suggests that the kinetics of formation of the network was very fast. IR and NMR studies revealed hydrogen bonding between amidic carbonyl in the side chain and hydroxyl groups of cholic acid.

1 aPore, Vandana, S.1 aAgalave, Sandip, G.1 aPharande, Shrikant, G.1 aPatil, Prashant, A.1 aKotmale, Amol, S. uhttps://library.ncl.res.in/content/bile-acid-hydrazides-gelation-structural-physical-and-spectroscopic-properties-002662nas a2200193 4500008004100000022001400041245013800055210006900193260007100262300001400333490000700347520187800354100001602232700003002248700002302278700002302301700002202324856012202346 2015 eng d a0743-746300aBioactive polymersomes self-assembled from amphiphilic.PPO-glycopolypeptides: synthesis, characterization, and dual-dye encapsulation0 aBioactive polymersomes selfassembled from amphiphilicPPOglycopol a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcAPR a3402-34120 v313 a

Glycopolypeptide-based polymersomes have promising applications as vehicles for targeted drug delivery because they are capable of encapsulating different pharmaceuticals of diverse polarity as well as interacting with specific cell surfaces due to their hollow structural morphology and bioactive surfaces. We have synthesized glycopolypeptide-b-poly(propylene oxide) by ROP of glyco-N-carboxyanhydride (NCA) using the hydrophobic amine-terminated poly(propylene oxide) (PPO) as the initiator. This block copolymer is composed of an FDA-approved PPO hydrophobic block in conjugation with hydrophilic glycopolypeptides which are expected to be biocompatible. We demonstrate the formation of glycopolypeptide-based polymersomes from the self-assembly of glycopolypeptide-b-poly(propylene oxide) in which the presence of an ordered helical glycopolypeptide segment is required for their self-assembly into spherical nanoscale (similar to 50 nm) polymersomes. The polymersomes were characterized in detail using a variety of techniques such as TEM, AFM, cryo-SEM, and light-scattering measurements. As a model for drugs, both hydrophobic (RBOE) and hydrophilic (calcein) dyes have been incorporated within the polymersomes from solution. To substantiate the simultaneous entrapment of the two dyes, spectrally resolved fluorescence microscopy was performed on the glycopeptide polymersomes cast on a glass substrate. We show that it is possible to visualize individual nanoscale polymersomes and effectively probe the dyes colocalization and energy-transfer behaviors therein as well as investigate the variation in dual-dye encapsulation over a large number of single polymersomes. Finally, we show that the galactose moieties present on the surface can specifically recognize lectin RCA(120), which reveals that the polymersomes surface is indeed biologically active.

1 aDas, Soumen1 aSharma, Dharmendar, Kumar1 aChakrabarty, Suman1 aChowdhury, Arindam1 aGupta, Sayam, Sen uhttps://library.ncl.res.in/content/bioactive-polymersomes-self-assembled-amphiphilicppo-glycopolypeptides-synthesis-002327nas a2200253 4500008004100000022001400041245008200055210006900137260000900206300001200215490000700227520152500234653001801759653001901777653001701796653001501813653002101828653001801849100002201867700002201889700002201911700002701933856011301960 2015 eng d a0928-493100aBioactive thermoresponsive polyblend nanofiber formulations for wound healing0 aBioactive thermoresponsive polyblend nanofiber formulations for  cMAR  a126-1370 v483 a

The rationale of this work is to develop new bioactive thermoresponsive polyblend nanofiber formulations for wound healing (topical). Various polymer compositions of thermoresponsive, poly(N-isopropylacrylamide), egg albumen and poly(epsilon-caprolactone) blend solutions with and without a drug [gatifloxacin hydrochloride, Gati] were prepared. Non-woven nanofibers of various compositions were fabricated using an electrospinning technique. The morphology of the nanofibers was analyzed by an environmental scanning electron microscope. The morphology was influenced by the concentration of polymer, drug, and polymer blend composition. Fourier transform infrared spectroscopy analysis showed the shift in bands due to hydrogen ion interactions between polymers and drug. Thermogram of PNIPAM/PCL/EA with Gati recorded a shift in lower critical solution temperature (LCST) and glass transition temperature (T-g) of PNIPAM. Similarly T-g and melting temperature (T-m) of PCL were shifted. X-ray diffraction patterns recorded a decrease in the crystalline state of PCL nanofibers and transformed crystalline drug to an amorphous state. In vitro release study of nanofibers with Gati showed initial rapid release up to 10 h, followed by slow and controlled release for 696 h (29 days). Nanofiber mats with Gati exhibited antibacterial properties to Staphylococcus aureus, supported suitable controlled drug release with in vitro cell viability and in vivo wound healing. (C) 2014 Elsevier B.V. All rights reserved.

10aAntibacterial10aCell viability10adrug release10ananofibers10athermoresponsive10awound Healing1 aPawar, Mahesh, D.1 aRathna, G., V. N.1 aAgrawal, Shubhang1 aKuchekar, Bhanudas, S. uhttps://library.ncl.res.in/content/bioactive-thermoresponsive-polyblend-nanofiber-formulations-wound-healing01981nas a2200181 4500008004100000022001400041245010600055210006900161260011000230300001400340490000600354520122700360100002001587700002001607700001701627700002601644856012901670 2015 eng d a2046-206900aBiocatalyst development for lactic acid production at acidic pH using inter-generic protoplast fusion0 aBiocatalyst development for lactic acid production at acidic pH  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcDEC a2024-20310 v53 a

Global warming and environmental problems force us to develop sustainable processes based on the use of biocatalysts that are eco-friendly with the least potential toxicity. Lactic acid fermentation at neutral pH generates a large amount of gypsum during down-stream processing. Hence it is essential to develop Lactobacillus strains which produce lactic acid at acidic pH thus making the whole downstream process environmentally friendly. Fusant F3 was generated using protoplast fusion between Lactobacillus delbrueckii Mut Uc-3 and Acetobacter pasteurianus NCIM 2314 on solid media at pH 4.0. Fusant F3 was further treated by UV irradiation to generate a mutant, FM1, with improvements in acid tolerance which produced five-fold more lactic acid than the parent strain at acidic pH. The molecular studies using RAPD markers demonstrated that the fusant is derived from both the parental strains, Acetobacter and Lactobacillus and the mutant is derived from the fusant. The utilization of such acid tolerant strains could be able to produce free lactic acid at acidic pH without using neutralizing agents and will offer an effective means for designing environmentally benign processes for lactic acid production.

1 aSinghvi, Mamata1 aGurjar, Gayatri1 aGupta, Vidya1 aGokhale, Digambar, V. uhttps://library.ncl.res.in/content/biocatalyst-development-lactic-acid-production-acidic-ph-using-inter-generic-protoplast-002146nas a2200241 4500008004100000022001400041245008700055210006900142260007300211300001200284490000700296520129500303653002001598653002001618653002201638653001401660100001601674700002701690700002301717700002101740700002701761856011601788 2015 eng d a0141-813000aBiochemical characterization of recombinant mevalonate kinase from Bacopa monniera0 aBiochemical characterization of recombinant mevalonate kinase fr aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN a776-7830 v723 a

Mevalonate kinase (MK; ATP: mevalonate 5-phosphotransferase; EC 2.7.1.36) plays a key role in isoprenoid biosynthetic pathway in plants. MK catalyzes the phosphorylation of mevalonate to form mevalonate-5-phosphate. The recombinant BmMK was cloned and over-expressed in E. coli BL21 (DE3), and purified to homogeneity by affinity chromatography followed by gel filtration. Optimum pH and temperature for forward reaction was found to be 7.0 and 30 degrees C, respectively. The enzyme was most stable at pH 8 at 25 degrees C with deactivation rate constant (Kd*) 1.398 x 10(-4) and half life (t(1/2)) 49 h. pH activity profile of BmMK indicates the involvement of carboxylate ion, histidine, lysine, arginine or aspartic acid at the active site of enzyme. Activity of recombinant BmMK was confirmed by phosphorylation of RS-mevalonate in the presence of Mg2+, having K-m and V-max 331.9 mu M and 719.1 pKat mu g(-1), respectively. The values of k(cat) and k(cat)/K-m for RS-mevalonate were determined to be 143.82 s(-1) and 0.43332 M-1 s(-1) and k(cat) and k(cat)/K-m values for ATP were found 150.9 s(-1) and 1.023 M-1 s(-1). The metal ion studies suggested that BmMK is a metal dependent enzyme and highly active in the presence of MgCl2. (C) 2014 Elsevier B.V. All rights reserved.

10aBacopa monniera10aEnzyme kinetics10aMevalonate kinase10astability1 aKumari, Uma1 aVishwakarma, Rishi, K.1 aSonawane, Prashant1 aAbbassi, Shakeel1 aKhan, Bashir, Mohammad uhttps://library.ncl.res.in/content/biochemical-characterization-recombinant-mevalonate-kinase-bacopa-monniera-002746nas a2200265 4500008004100000022001400041245011600055210006900171260007300240300001400313490000900327520177500336653001902111653002302130653002202153653002002175653002502195100002002220700002402240700002302264700002102287700002602308700002002334856012602354 2015 eng d a0304-416500aBiochemical, structural and functional diversity between two digestive alpha-amylases from helicoverpa armigera0 aBiochemical structural and functional diversity between two dige aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcSEP a1719-17280 v18503 a

Background: Helicoverpa armigera (Lepidoptera) feeds on various plants using diverse digestive enzymes as one of the survival tool-kit. The aim of the present study was to understand biochemical properties of recombinant alpha-amylases of H. armigera viz., HaAmy1 and HaAmy2. Methods: The open reading frames of HaAmy1 and HaAmy2 were cloned in Pichia pastoris and expressed heterologously. Purified recombinant enzymes were characterized for their biochemical and biophysical attributes using established methods. Results: Sequence alignment and homology modeling showed that HaAmy1 and HaAmy2 were conserved in their amino acid sequences and structures. HaAmy1 and HaAmy2 showed optimum activity at 60 degrees C; however, they differed in their optimum pH. Furthermore, HaAmy2 showed higher affinity for starch and amylopectin whereas HaAmy1 had higher catalytic efficiency. HaAmy1 and HaAmy2 were inhibited to the same magnitude by a synthetic amylase inhibitor (acarbose) while wheat amylase inhibitor showed about 2-fold higher inhibition of HaAmy1 than HaAmy2 at pH 7 while 6-fold difference at pH 11. Interactions of HaAmy1 and HaAmy2 with wheat amylase inhibitor revealed 2:1 stoichiometric ratio and much more complex interaction with HaAmy1. Conclusions: The diversity of amylases in perspective of their biochemical and biophysical properties, and their differential interactions with amylase inhibitors signify the potential role of these enzymes in adaptation of H. armigera on diverse plant diets. General significance: Characterization of digestive enzymes of H. armigera provides the molecular basis for the polyphagous nature and thus could assist in designing future strategies for the insect control. (C) 2015 Elsevier B.V. All rights reserved.

10aalpha-amylases10aAmylase inhibitors10aDigestive enzymes10aEnzyme activity10aHelicoverpa armigera1 aBhide, Amey, J.1 aChannale, Sonal, M.1 aPatil, Sucheta, S.1 aGupta, Vidya, S.1 aRamasamy, Sureshkumar1 aGiri, Ashok, P. uhttps://library.ncl.res.in/content/biochemical-structural-and-functional-diversity-between-two-digestive-alpha-amylases-002471nas a2200205 4500008004100000022001400041245008700055210006900142260007100211300001400282490000700296520170000303100001902003700001902022700003102041700002202072700002202094700002902116856012002145 2015 eng d a0897-475600aBioinspired catecholic flame retardant nanocoating for flexible polyurethane foams0 aBioinspired catecholic flame retardant nanocoating for flexible  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcOCT a6784-67900 v273 a

An efficient, environmentally friendly, and water-applied flame retardant surface nanocoating based on polydopamine (PDA) was developed for foamed materials such as polyurethane (PU). The PDA nanocoating, deposited by simple dip-coating in an aqueous dopamine solution, consists of a planar sublayer and a secondary granular layer structure that evolve together, eventually turning into a dense, uniform, and conformal layer on all foam surfaces. In contrast to flexible PU foams that are known to be highly flammable without flame retardant additives, micro combustion calorimetry (MCC) and thermogravimetric analysis (TGA) confirm that the neat PDA is relatively inflammable with a strong tendency to form carbonaceous, porous char that is highly advantageous for flame retardancy. By depositing nano coatings of PDA onto flexible PU foams, the flammability of the PU foam was significantly reduced with increasing coating thickness. For the thickest coating (3 days of PDA deposition), the foam quickly self-extinguished and its original shape was completely preserved after exposure to a flame in a torch burn test. In addition to the char forming ability of PDA, it is hypothesized that its catechol units likely scavenge nearby radicals that typically evolve additional fuel for the fire as they attack surrounding materials. This multiple flame retardancy action of PDA (i.e., char formation + radical scavenging) enables flame retardant foams with a peak heat release rate (P-HRR) that is significantly reduced (up to 67%) relative to control foams, representing much better performance than many conventional additives reported in the literature at comparable or higher loadings.

1 aCho, Joon, Hee1 aVasagar, Vivek1 aShanmuganathan, Kadhiravan1 aJones, Amanda, R.1 aNazarenko, Sergei1 aEllison, Christopher, J. uhttps://library.ncl.res.in/content/bioinspired-catecholic-flame-retardant-nanocoating-flexible-polyurethane-foams-001691nas a2200169 4500008004100000022001400041245012100055210006900176260011000245300001600355490000600371520094400377100002201321700002101343700002501364856013201389 2015 eng d a2046-206900aBiomass derived beta-cyclodextrin-SO3H as a solid acid catalyst for esterification of carboxylic acids with alcohols0 aBiomass derived betacyclodextrinSO3H as a solid acid catalyst fo aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJAN a12981-129860 v53 a

A novel beta-cyclodextrin-SO3H carbon based solid acid catalyst was prepared in a convenient and ecofriendly manner and was characterized using FTIR, PXRD, EDAX and NH3TPD to illustrate that the carbon material has been functionalized with -SO3H, -COOH and -OH groups. The catalyst was studied for esterification of various carboxylic acids and alcohols under solvent free conditions and showed excellent catalytic performance and gave good yields of esters in the range 78-99% at 70 degrees C. No solvent was used either for catalyst preparation nor for esterification reaction. The catalyst can be easily recovered by simple filtration and reused for subsequent three runs without any significant impact on yields of products. The main advantage of this methodology is easy and ecofriendly catalyst preparation, easy catalyst separation, practical simplicity, safe reaction conditions, recyclable catalyst and high product yields.

1 aThombal, Raju, S.1 aJadhav, Amol, R.1 aJadhav, Vrushali, H. uhttps://library.ncl.res.in/content/biomass-derived-beta-cyclodextrin-so3h-solid-acid-catalyst-esterification-carboxylic-acids-001906nas a2200157 4500008004100000022001400041245006600055210006400121260010200185300001400287490000800301520129300309100002401602700002601626856009601652 2015 eng d a0011-389100aBiomass exploitation - a challenge finding its way to reality0 aBiomass exploitation a challenge finding its way to reality aC V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIAbINDIAN ACAD SCIENCEScMAY a1593-15940 v1083 a

Lignocellulosic biomass holds the key to supplying the basic needs of society for sustainable production of chemicals and fuels without impacting the human food supply. The production of second-generation biofuels and chemicals from lignocellulosic biomass has not yet been commercialized due to its complex and recalcitrance structure. Therefore, the challenges involved in the production of lignocellulosic biomass-derived fuels and chemicals must be addressed. Search for economic pretreatment methods has been recognized as one of the main hurdles for processing of biomass to biofuels and chemicals. The conversion of all biomass components, lignin in particular, would greatly contribute to the economic viability of biomass-based processes for second-generation biofuels and chemicals. Hydrolysis of lignocellulose carbohydrates into fermentable sugars requires a suitable cellulase enzyme cocktail acting on both raw as well as pretreated biomass. Depending on raw material and pretreatment technology, the enzyme mixtures must be designed to degrade biomass carbohydrates. Recent publications on GVL-pretreatment to solubilize and degrade carbohydrates in biomass and CelA enzyme acting on raw biomass would probably meet the challenges in biomass conversion technologies.

1 aSinghvi, Mamata, S.1 aGokhale, Digambar, V. uhttps://library.ncl.res.in/content/biomass-exploitation-challenge-finding-its-way-reality-002647nas a2200169 4500008004100000022001400041245011600055210006900171260011000240300001400350490000600364520191700370100002702287700002302314700001902337856012102356 2015 eng d a2046-206900aBiomilling of rod-shaped ZnO nanoparticles: a potential role of saccharomyces cerevisiae extracellular proteins0 aBiomilling of rodshaped ZnO nanoparticles a potential role of sa aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcNOV a1883-18890 v53 a

There is a tremendous interest in newly-discovered, green, room-temperature, biological routes for the fabrication of biologically-benign functional nanostructures. The bottom-up biogenic synthesis, where the precursor molecules form crystalline solids at the nanoscale by a redox process, has been validated over the years and gained its popularity. However, a new top-down technique has recently been developed by our group, in which small isotropic nanoparticles (NPs) are formed by the break-down of chemically-synthesized anisotropic rod or plate-shaped NPs using microbes (termed as biomilling). This technique, which holds great promise, is still in its infancy. Here, an improved process with an easy isolation of NPs from the biomass and better control of the technique is reported. This novel technique is demonstrated to break-down the chemically synthesized ZnO nanorods (NRs), similar to 250 nm in length, to small quasi-spherical ZnO NPs (similar to 10 nm in diameter) possibly due to the proteins secreted by the yeast (Saccharomyces cerevisiae), which also led to the formation of `` corona'' around the NPs. The UV-vis, PL and FTIR results show the dynamic nature of the protein corona, which is further supported by the SDS-PAGE study of the extracellular proteins. The SDS-PAGE study of the intracellular proteins shows the over-expression of a single protein which is supposed to have a role in zinc transport in the cells. The ICP-OES results show the accumulation of a higher amount of zinc in the yeast cells as biomilling progresses, while the extracellular zinc contents were almost same. Therefore, we believe that the yeast cells play an important role in the biomilling process by secreting the proteins and maintaining the zinc content in the extracellular fluid. The biomilled NPs exhibit a uniform dispersity and better aqueous stability than chemically synthesized ZnO NRs.

1 aSharan, Chandrashekhar1 aKhandelwal, Puneet1 aPoddar, Pankaj uhttps://library.ncl.res.in/content/biomilling-rod-shaped-zno-nanoparticles-potential-role-saccharomyces-cerevisiae-001166nas a2200157 4500008004100000022001400041245016200055210006900217260007100286300001400357490000700371520046500378100002200843700001900865856012400884 2015 eng d a1523-706000aBiomimetic synthesis of phaitanthrin e involving a fragmentation of sp(3) carbon-carbon bond: synthesis and rearrangement of phaitanthrin D to phaitanthrin E0 aBiomimetic synthesis of phaitanthrin e involving a fragmentation a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcDEC a6218-62210 v173 a

A biogenetic type total synthesis of alkaloids phaitanthrin D and phaitanthrin E has been described. The Csp(3)-Csp(3) bond cleavage with the release of several heteroatoms bearing unexpected leaving groups in intramolecular substitution reactions on an iminium double bond in the quinazolinones has been demonstrated using HMDS/ZnCl2 or NaHMDS. The mechanistic aspects have been supported by isolation and characterization of appropriate intermediates.

1 aVaidya, Sagar, D.1 aArgade, N., P. uhttps://library.ncl.res.in/content/biomimetic-synthesis-phaitanthrin-e-involving-fragmentation-sp3-carbon-carbon-bond-002698nas a2200229 4500008004100000022001400041245013900055210006900194260007100263300001200334490000700346520180400353100002302157700002602180700002802206700002102234700002302255700002202278700002602300700002402326856011802350 2015 eng d a1525-779700aBiophysical characterization and molecular docking studies of imidazolium based polyelectrolytes-DNA complexes: role of hydrophobicity0 aBiophysical characterization and molecular docking studies of im a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcMAR a894-9030 v163 a

Nonviral gene delivery vectors are acquiring greater attention in the field of gene therapy by replacing the biological viral vectors. DNA-cationic polymer complexes are one of the most promising systems to find application in gene therapy. Hence, a complete insight of their biophysical characterization and binding energy profile is important in understanding the mechanism involved in nonviral gene therapy. In this investigation, the interaction between calf thymus DNA (ctDNA) and imidazolium-based poly(ionic liquids) (PILs) also known as polyelectrolytes with three different alkyl side chains (ethyl, butyl, and hexyl) in physiological conditions using various spectroscopic experiments with constant DNA concentration and varying polyelectrolyte concentrations is reported. UV-visible absorption, fluorescence quenching studies, gel electrophoresis, circular dichroism (CD), and Fourier transform infrared spectroscopy (FTIR) have confirmed the binding of polyelectrolytes with DNA. UV-vis absorption measurements and fluorescence quenching revealed that the binding between DNA and the polyelectrolyte is dominated by electrostatic interactions. Additionally, CD and FTIR results indicated that the DNA retained its B-form with minor perturbation in the phosphate backbone without significant change in the conformation of its base pairs. Preference for alkyl side chains (KPIL-Ethyl Br < KPIL-Butyl Br < KPIL-Hexyl Br) toward efficient binding between the polyelectrolyte and DNA was inferred from the binding and quenching constants calculated from the absorption and emission spectra, respectively. Further, in silico molecular docking studies not only validated the observed binding trend but also provided insight into the binding mode of the polyelectrolyte-DNA complex.

1 aManojkumar, Kasina1 aCharan, K., T. Prabhu1 aSivaramakrishna, Akella1 aJha, Prakash, C.1 aKhedkar, Vijay, M.1 aSiva, Ramamoorthy1 aJayaraman, Gurunathan1 aVijayakrishna, Kari uhttps://library.ncl.res.in/content/biophysical-characterization-and-molecular-docking-studies-imidazolium-based-002373nas a2200265 4500008004100000022001400041245011300055210006900168260007300237300001000310490000800320520140200328653001601730653002401746653002101770653002501791653002301816100002101839700002401860700002501884700002501909700002401934700002001958856012901978 2015 eng d a0927-776500aBio-physical evaluation and in vivo delivery of plant proteinase inhibitor immobilized on silica nanospheres0 aBiophysical evaluation and in vivo delivery of plant proteinase  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUN a84-920 v1303 a

Recombinant expression of Capsicum annuum proteinase inhibitors (CanPI-13) and its application via synthetic carrier for the crop protection is the prime objective of our study. Herein, we explored proteinase inhibitor peptide immobilization on silica based nanospheres and rods followed by its pH mediated release in vitro and in vivo. Initial studies suggested silica nanospheres to be a suitable candidate for peptide immobilization. Furthermore, the interactions were characterized biophysically to ascertain their conformational stability and biological activity. Interestingly, bioactive peptide loading at acidic pH on nanospheres was found to be 62% and showed 56% of peptide release at pH 10, simulating gut milieu of the target pest Helicoverpa armigera. Additionally, in vivo study demonstrated significant reduction in insect body mass (158 mg) as compared to the control insects (265 mg) on 8th day after feeding with CanPI-13 based silica nanospheres. The study confirms that peptide immobilized silica nanosphere is capable of affecting overall growth and development of the feeding insects, which is known to hamper fecundity and fertility of the insects. Our study illustrates the utility and development of peptide-nanocarrier based platform in delivering diverse biologically active complexes specific to gut pH of H. armigera. (C) 2015 Elsevier B.V. All rights reserved.

10aBiodelivery10aHelicovera armigera10aProtein `corona'10aProteinase inhibitor10aSilica nanospheres1 aKhandelwal, Neha1 aDoke, Dhananjay, S.1 aKhandare, Jayant, J.1 aJawale, Priyanka, V.1 aBiradar, Ankush, V.1 aGiri, Ashok, P. uhttps://library.ncl.res.in/content/bio-physical-evaluation-and-vivo-delivery-plant-proteinase-inhibitor-immobilized-silica-002082nas a2200217 4500008004100000022001400041245012000055210006900175260007900244300001200323490000800335520125300343653001601596653002201612653001701634653002001651653002301671100001801694700002501712856012701737 2015 eng d a0273-228900aBiophysicochemical characterization of an alkaline protease from beauveria sp. MTCC 5184 with multiple applications0 aBiophysicochemical characterization of an alkaline protease from a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcJAN a589-6020 v1753 a

This study illustrates the biophysicochemical properties of an alkaline protease, BAP (Beauveria sp. alkaline protease) from Beauveria sp. MTCC 5184. This protease exhibited maximum activity at 50 degrees C, pH 9.0, and stability in a broad pH range, in the presence of organic solvents, denaturants, as well as detergents. Wash performance studies revealed that BAP was able to remove blood clots/stains from blood-soaked cloth. Peptide mass fingerprinting results demonstrated partial homology of BAP with subtilisin-like proteinase. BAP showed catalytic activity against natural as well as synthetic substrates. Active site characterization of BAP confirmed the involvement of serine, tryptophan, and aspartic acid in catalytic activity. Detailed kinetic and thermodynamic studies of BAP demonstrated that the activation energy (Ea) for casein hydrolysis was 82.55 kJ/M, the specificity constant (Kcat/K-m), and the values of Delta G (change in Gibbs free energy) decreased with increase in temperature, whereas Delta H (change in enthalapy) and Delta S (change in entropy) were constant. The results of the present study indicate that BAP has potential for applications as detergent additive, in peptide synthesis, and in basic research.

10aActive site10aAlkaline Protease10aBeauveria sp10aOrganic solvent10aSubstrate kinetics1 aShankar, Shiv1 aLaxman, Ryali, Seeta uhttps://library.ncl.res.in/content/biophysicochemical-characterization-alkaline-protease-beauveria-sp-mtcc-5184-multiple-002128nas a2200277 4500008004100000022001400041245007700055210006900132260008800201300001200289490000700301520119600308653001701504653001401521653002301535653002501558653002801583100001701611700002001628700002401648700001801672700001701690700001601707700001901723856010801742 2015 eng d a0217-984900aBiosynthesis of silver nanoparticles by using ganoderma-mushroom extract0 aBiosynthesis of silver nanoparticles by using ganodermamushroom  a5 TOH TUCK LINK, SINGAPORE 596224, SINGAPOREbWORLD SCIENTIFIC PUBL CO PTE LTDcMAR a15400470 v293 a

Present study reports the biochemical synthesis of silver nanoparticles (Ag-NPs) from aqueous medium by using the extract of medicinal mushroom Ganoderma, as a reducing and stabilizing agents. The Ag-NPs are prepared at room temperature by the reduction of Ag+ to Ag in aqueous solution of AgNO3. The resultant particles are characterized by using UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy and transmission electron microscopy (TEM) measurement techniques. The formation of Ag-NPs is confirmed by recording the UV-visible absorption spectra for surface plasmon resonance (SPR) where peak around 427 nm. The prominent changes observed in FTIR spectra supported the reduction of Ag+ to Ag. The morphological features of Ag-NPs are evaluated from HRTEM. The spherical Ag-NPs are observed in transmission electron microscopy (TEM) studies. The particle size distribution is found to be nearly uniform with average particle size of 2 nm. The Ag-NPs aged for 15, 30, 60 and 120 days showed no profound effect on the position of SPR peak in UV-visible studies, indicating the protecting/capping ability of medicinal mushroom Ganoderma in the synthesis of AgNPs.

10aBiosynthesis10aGanoderma10amedicinal mushroom10asilver nanoparticles10aUV-visible spectroscopy1 aEkar, S., U.1 aKhollam, Y., B.1 aKoinkar, Pankaj, M.1 aMirji, S., A.1 aMane, R., S.1 aNaushad, M.1 aJadhav, S., S. uhttps://library.ncl.res.in/content/biosynthesis-silver-nanoparticles-using-ganoderma-mushroom-extract-101614nas a2200157 4500008004100000245006000041210005900101260008100160300001000241520101200251100002401263700002401287700002801311700002301339856009401362 2015 eng d00aBlack phosphorous nanosheets: prospective field emitter0 aBlack phosphorous nanosheets prospective field emitter aGuangzhou, Peoples R ChinabIEEE, 345 E 47th St, New York, NY 10017 USAcJUL a94-953 a

We report for the first time field electron emission investigations on few layered black phosphorous nanosheets emitter's synthesized using chemical method with thickness similar to 2-5 nm. The turn-on electric field required to draw an emission current density of similar to 10 mu A/cm(2) was found to be similar to 4.2 V/mu m. Furthermore, a few layer black phosphorous nanosheets emitter deliver an large emission current density of similar to 170 mu A/cm(2) at an applied field of similar to 7.5 V/mu m. The emission current versus time plot measured at the preset current values of similar to 5 mu A shows field emission current fluctuations within +/- 10% of the average value indicating the well stable nature of black phosphorous field emitter. Thus, the two dimensional black phosphorous nanosheets synthesized using simple electrochemical exfoliation method can be realized and utilized for the next generation micro/nanoelectronics and flat panel field emission based display applications.

1 aLate, Dattatray, J.1 aErande, Manisha, B.1 aSuryawanshi, Sachin, R.1 aMore, Mahendra, A. uhttps://library.ncl.res.in/content/black-phosphorous-nanosheets-prospective-field-emitter02401nas a2200241 4500008004100000022001400041245006600055210006600121260008700187300001200274490000700286520154300293653003101836653001501867653001201882653002101894653002401915100002901939700003401968700003502002700002902037856009302066 2015 eng d a0883-911500aBlends of shellac as nanofiber formulations for wound healing0 aBlends of shellac as nanofiber formulations for wound healing a1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLANDbSAGE PUBLICATIONS LTDcSEP a472-4890 v303 a

Shellac is being used in food, pharmaceutical, and agricultural industries. It is seldom used for biomedical applications due to its poor mechanical property and instability. We designed thermoresponsive shellac-based bioactive nanofiber mats that mimic extracellular matrix to extend their utility in wound healing. Various blend compositions of shellac, gelatin, and poly(N-isopropylacrylamide) enriched with a bioactive agent, nadifloxacin, were prepared and nanofiber mats were fabricated. The morphology of the nanofiber formation was influenced by the concentration of polymer, drug, and polymer blend composition. Polymer-drug interactions and thermal and crystalline properties of nanofiber mats were analyzed. The shellac/gelatin/poly(N-isopropylacrylamide) blend of composition 3%/7%/3% (w/v) was chosen to evaluate in vitro drug release. Release studies recorded slow, constant, and sustained release for 140h. The release kinetics and mechanism confirmed zero-order release with resultant r(2) values greater than 0.99, and the Korsmeyer-Peppas release exponent (n) was slightly higher than 0.8, which indicated that drug diffusion was anomalous or non-Fickian type and drug release followed diffusion involving chain stretching. Indirect cytotoxicity studies recorded insignificant toxicity against normal human fibroblast cells. In vivo studies demonstrated that drug-loaded nanofiber mats were more suitable for faster tissue regeneration as compared to nanofiber mats without drug and commercial nadifloxacin cream.

10adrug release and mechanism10ananofibers10aShellac10athermoresponsive10atissue regeneration1 aVannuruswamy, Garikapati1 aRathna, Gundloori, Venkata Na1 aGadgil, Bhagyashri, Shashikant1 aGadad, Anand, Panchaksha uhttps://library.ncl.res.in/content/blends-shellac-nanofiber-formulations-wound-healing-001218nas a2200169 4500008004100000022001400041245016100055210006900216260007100285300001400356490000700370520048500377100001800862700001700880700002100897856013000918 2015 eng d a1523-706000aBreaking and making of olefins simultaneously using ozonolysis: application to the synthesis of useful building blocks and macrocyclic core of solomonamides0 aBreaking and making of olefins simultaneously using ozonolysis a a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcMAY a2090-20930 v173 a

A simple and practical one-pot) two-directional approach to access olefinic esters through simultaneous breaking and making of olefins using ozonolysis of alkenyl aryl selenides is disclosed The scope of the method with a variety of example's is demonstrated, and the end products obtained here are useful building blocks. As a direct application of the present method,the macrocyclic cote of potent anti-inflammatory natural cyclic peptides, solomonamides, is synthesized.

1 aKashinath, K.1 aDhara, Santu1 aReddy, Srinivasa uhttps://library.ncl.res.in/content/breaking-and-making-olefins-simultaneously-using-ozonolysis-application-synthesis-useful-001263nas a2200217 4500008004100000022001400041245013600055210006900191260007500260300001400335520042400349653001100773653001600784653002300800653001400823653001500837100001800852700002700870700002100897856012700918 2015 eng d a1434-193X00aBreaking and making of rings: a method for the preparation of 4-quinolone-3-carboxylic acid amides and the expensive drug ivacaftor0 aBreaking and making of rings a method for the preparation of 4qu aPOSTFACH 101161, 69451 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcDEC a7433-74373 a

A simple and convenient method to access 4-quinolone-3-carboxylic acid amides from indole-3-acetic acid amides through one-pot oxidative cleavage of the indole ring followed by condensation (Witkop-Winterfeldt type oxidation) was explored. The scope of the method was confirmed with more than 20 examples and was successfully applied to the synthesis of the drug Ivacaftor, the most expensive drug on the market.

10aAmides10aDrug design10aFused-ring systems10aOxidation10aOzonolysis1 aVasudevan, N.1 aJachak, Gorakhnath, R.1 aReddy, Srinivasa uhttps://library.ncl.res.in/content/breaking-and-making-rings-method-preparation-4-quinolone-3-carboxylic-acid-amides-and-001353nas a2200157 4500008004100000022001400041245008300055210006900138260011000207300001200317490000600329520069100335100002801026700002901054856011201083 2015 eng d a2044-475300aBronsted acidic ionic liquid-catalyzed conversion of hemicellulose into sugars0 aBronsted acidic ionic liquidcatalyzed conversion of hemicellulos aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcSEP a531-5390 v53 a

Development of a competent method for the conversion of hemicellulose, a lignocellulosic component, into sugars is essential. In a one-pot method, Bronsted acidic ionic liquid (BAIL)-catalyzed hydrolysis of hardwood hemicellulose at 160 degrees C in water media gave C5 sugars (xylose + arabinose) in 87% yield. The efficiency of the ILs and their acid strength both followed the similar trend, [C3SO3HMIM][HSO4] > [C3SO3HMIM][PTS] > [C3SO3HMIM][Cl] > [BMIM][Cl]. The ion-dipole-type interaction present between the BAIL and the substrate, which is proposed by the H-1 NMR study, is suggested to help in achieving better activity with BAIL than with mineral acid, H2SO4.

1 aMatsagar, Babasaheb, M.1 aDhepe, Paresh, Laxmikant uhttps://library.ncl.res.in/content/bronsted-acidic-ionic-liquid-catalyzed-conversion-hemicellulose-sugars-002035nas a2200229 4500008004100000022001400041245006600055210006400121260005700185300001000242490000800252520127000260653001601530653001401546653002001560653002401580100002501604700002401629700003001653700002601683856009601709 2016 eng d a0302-893300aBacillus cellulasensis sp nov., isolated from marine sediment0 aBacillus cellulasensis sp nov isolated from marine sediment a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJAN a83-890 v1983 a

A novel bacterial strain NIO-1130(T) was isolated from sediment sample taken from Chorao Island, Goa Province, India, and subjected to a taxonomic investigation. The strain was Gram-positive, aerobic, and motile. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and strain NIO-1130(T) showed highest sequence similarity with Bacillus halosaccharovorans DSM 25387(T) (98.4 %) and Bacillus niabensis CIP 109816(T) (98.1 %), whereas other Bacillus species showed < 97.0 % similarity. Tree based on gyrB gene sequence revealed that strain bacillus group. The major menaquinone was MK-7 and the predominant cellular fatty acids were iso-C-15:0, anteiso-C-15:0, iso-C-17:0, and anteiso-C-17:0. The strain showed a DNA G+C content of 39.9 mol%. DNA-DNA hybridization studies revealed that strain NIO-1130(T) exhibits 70 % similarity with Bacillus halosaccharovorans DSM 25387(T) and Bacillus niabensis CIP 109816T. On the basis of physiological, biochemical, chemotaxonomic and phylogenetic analyses, we consider the isolate to represent a novel species of the genus Bacillus, for which the name Bacillus cellulasensis sp. nov., is proposed. The type strain is NIO-1130(T) (=NCIM 5461(T) = CCTCC AB 2011126(T)).

10aBacillus sp10aCellulase10aMarine sediment10aPolyphasic taxonomy1 aMawlankar, Rahul, R.1 aThorat, Meghana, N.1 aKrishnamurthi, Srinivasan1 aDastager, Syed, Gulam uhttps://library.ncl.res.in/content/bacillus-cellulasensis-sp-nov-isolated-marine-sediment-002108nas a2200169 4500008004100000245005000041210004700091260003800138300001200176520157700188100002101765700001501786700002001801700001801821700001801839856008101857 2016 eng d00aBalancing omega-6: Omega-3 ratios in oilseeds0 aBalancing omega6 Omega3 ratios in oilseeds bSpringer International Publishing a203-2203 aFats and oils are the essential constituents of human diet, and nearly 80 % of these are obtained from plants. The predominant fatty acids present in plant oils are saturated and unsaturated compounds with straight aliphatic chains of carbon atoms and a single carboxyl group. Depending on the position of the first double bond from the methyl end in the fatty acyl chain, the mono- and polyunsaturated fatty acids can be denoted as -9, -6, or -3. Excess consumption of -6 fatty acids has greatly and unfavorably increased the -6: -3 ratio up to 25:1, which is associated with prevalence of many negative health effects, including cardiovascular diseases, cancer, osteoporosis, and inflammatory and autoimmune diseases. Because the -3 fatty acids (FAs) are the precursors for synthesis of anti-inflammatory eicosanoids, balancing the -6: -3 ratio is vital. The easiest approach to achieve this would be consumption of oils rich in -3 FAs, such as linseed oil. Alternatively, the fatty acid biosynthetic pathway in plants producing high -6 FAs can be altered by biotechnological means, so that they produce higher proportion of -3 FAs. This chapter describes the current knowledge of the fatty acid biosynthesis pathway in plants, including the genes involved, their temporal and spatial expression patterns, and various fluxes that they drive. The choice of oilseeds, genes, and promoters for modulating the fatty acid biosynthesis flux from -6 to -3 fatty acids is also dealt with. The potential hurdles in achieving these and possible solutions have also been described.1 aChirmade, T., P.1 aSanghi, S.1 aRajwade, A, .V.1 aGupta, V., S.1 aKadoo, N., Y. uhttps://library.ncl.res.in/content/balancing-omega-6-omega-3-ratios-oilseeds01905nas a2200169 4500008004100000022001400041245012300055210006900178260007500247300001200322490000600334520118700340100002501527700002401552700003001576856012901606 2016 eng d a1614-683200aBand edge modulated polymer layer to decrease back electron transfer and increase efficiency in sensitized solar cells0 aBand edge modulated polymer layer to decrease back electron tran aPOSTFACH 101161, 69451 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcMAY a15023340 v63 a

Recombination of charges residing in the TiO2 and redox electrolyte is one of the factors affecting the efficiency of dye sensitized solar cells (DSSCs). To circumvent this recombination, inorganic oxide barrier layers and organic silanes have been coated on TiO2/dyes. Due to the insulating nature of these layers, the efficiency increase is not very impressive. Conducting polymers with different band edges are used to suppress the charge recombination. Amongst the four polymers that are used as barrier layers, a polymer with a highest occupied molecular orbital energy at -5.8 eV and lowest unoccupied molecular orbital at -3.1 eV is found to increase the electron life time at TiO2 and decrease the charge recombination. The electron life time is found to be 88 ms. In addition to the long electron life time, the recombination resistance of this polymer is also high (91 Omega). This resistance is 18% higher than that measured for DSSCs without polymer barrier layer. These factors impact the efficiency of DSSCs. DSSCs fabricated with this polymer as barrier layer exhibit an efficiency of 9.2%, which is 22% higher than that of DSSCs without polymer barrier layer.

1 aArulkashmir, Arulraj1 aSudhakar, Vediappan1 aKrishnamoorthy, Kothandam uhttps://library.ncl.res.in/content/band-edge-modulated-polymer-layer-decrease-back-electron-transfer-and-increase-efficiency01657nas a2200229 4500008004100000022001400041245006000055210006000115260010200175300001200277490000800289520087100297653002401168653001201192653001601204653001401220653002701234100002201261700002701283700002601310856009101336 2016 eng d a0011-389100aBehaviour of biomass multicomponent ashes as adsorbents0 aBehaviour of biomass multicomponent ashes as adsorbents aC V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIAbINDIAN ACAD SCIENCEScJAN a180-1860 v1103 a

Physico-chemical characteristics of rice husk ash and baggase fly ash, commonly referred to as biomass ashes enable their use as adsorbents. Contrary to normal expectations, it is observed that larger particles have more number, narrower and deeper pores than smaller particles. As a consequence they have higher pore volume, total surface area and hence adsorption capacity. Also, the uptake rate of adsorption depends on the silica to carbon ratio, which is seen to be smaller for larger particles and hence they take a longer time to reach equilibrium. The extent of carbon content determines the capacity, whereas silica to carbon ratio determines the kinetics of adsorption. Removal of 2,4-dichlorophenoxyacetic acid, from aqueous solution was chosen as a representative case for study and the results obtained are compared with earlier reported results.

10aAdsorption capacity10abagasse10abiomass ash10arice husk10asilica to carbon ratio1 aDeokar, Sunil, K.1 aMandavgane, Sachin, A.1 aKulkarni, Bhaskar, D. uhttps://library.ncl.res.in/content/behaviour-biomass-multicomponent-ashes-adsorbents-001806nas a2200169 4500008004100000245013500041210007100176260000800247300001600255490000600271520113200277100001901409700002601428700002401454700001901478856013901497 2016 eng d00aBenz–amidinato stabilized a monomeric calcium Iodide and a lithium calciate(II) cluster featuring group 1 and group 2 elements0 aBenz–amidinato stabilized a monomeric calcium Iodide and a lithi cAPR a1066–10710 v13 a

Soluble calcium halides reported so far are mostly dimeric in nature. The halides occupy the bridging position and thus provide additional coordination to the metal. We obtained a monomeric calcium iodide [{PhC(NiPr)(2)} CaI(thf)(3)] (1) from the reaction of [PhC(NiPr)(2)] Li with Cal(2) in THF. The compound has been stabilized by electronic donation and steric shielding from the amidinate ligand as well as coordination of three THF molecules. 1 does not show any propensity towards ligand exchange reaction. When the same reaction is carried out in diethyl ether instead of THF, it led to the formation of a Li calciate(II) cluster of composition L2Ca4I8Li4O (L= PhC(NiPr)(2)) (2) with an encapsulated O2+ in the middle of a tetrahedron spanned by four Ca2+ ions. 2 represents a metal-rich halide comprising of both alkali and alkaline earth metals which is quite unprecedented. Another notable aspect is that the amidinate ligand binds to the calcium atom in chelating bidentate mode in 1, whereas in 2 each N atom of the amidinate ligands binds to two Ca atoms leading to bridging bis-chelating coordination mode.

1 aYadav, Sandeep1 aSwamy, V., S. V. S. N1 aGonnade, Rajesh, G.1 aSen, Sakya, S. uhttps://library.ncl.res.in/content/benz%E2%80%93amidinato-stabilized-monomeric-calcium-iodide-and-lithium-calciateii-cluster-featuring02754nas a2200277 4500008004100000022001400041245010100055210006900156260006900225300001200294490000800306520182100314653000802135653002302143653001602166653002402182653001102206653001202217100002502229700002402254700001702278700001502295700001802310700002002328856012802348 2016 eng d a0925-400500aBicinchoninic acid-based colorimetric chemosensor for detection of low concentrations of cyanide0 aBicinchoninic acidbased colorimetric chemosensor for detection o aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcJAN a112-1190 v2223 a

Bicinchoninic acid (BCA) is reagent that is widely used to determine the concentration of proteins in solutions through the colored complex it forms with Cu(I) and the proteins. In this report, we present a new use for BCA, where a highly sensitive method for detection of cyanide ions in solutions by using Cu2+-BCA complex as the chemosensing agent is demonstrated. The detection of cyanide ions is achieved by taking advantage of the nucleophilic attack of the Cu2+-BCA complex by cyanide ions. Upon the addition of cyanide ions, the Cu2+-BCA complex undergoes transformations to free BCA via a series of equilibriums and, as a result, forms different Cu(II)-(BCA)(x)(CN)(y) species depending on the concentrations of cyanide ions in the solution. These different Cu(II)-(BCA)(x)(CN)(y) species, in turn, give different metal-to-ligand charge transfer electronic spectra and colors that are easily detectable both with naked eyes and UV-vis spectroscopy. As the cyanide ion concentration increases, the color of the solution containing the complexes changes from green to purple, red, yellow, and finally colorless, giving a corresponding blue shift in the absorption maxima on their UV-vis spectra. Through this process, the complexes enable detection of cyanide ions with a detection level of up to 0.06 ppm in solutions. Thus, this colorimetric technique based on Cu2+-BCA can make low concentrations of cyanide detectable, well before the concentrations possibly reach lethal amounts. Furthermore, the method is shown to give fast detection response with no interference from other anionic and cationic species and has the potential to be adopted for accurate and convenient analysis of cyanide ions in drinking water sources as well as industrial effluents. (C) 2015 Elsevier B.V. All rights reserved.

10aBCA10aBicinchoninic acid10aChemosensor10aColorimetric sensor10aCopper10aCyanide1 aBiradar, Archana, A.1 aBiradar, Ankush, V.1 aSun, Tiffany1 aChan, Yung1 aHuang, Xiaoxi1 aAsefa, Tewodros uhttps://library.ncl.res.in/content/bicinchoninic-acid-based-colorimetric-chemosensor-detection-low-concentrations-cyanide-000407nas a2200121 4500008004100000245005700041210005600098260000800154300000800162490000600170100002100176856008800197 2016 eng d00aBig Data Deluge in Biology: Challenges and Solutions0 aBig Data Deluge in Biology Challenges and Solutions cJUN a1-30 v11 aSarkar, Ram, Rup uhttps://library.ncl.res.in/content/big-data-deluge-biology-challenges-and-solutions02864nas a2200229 4500008004100000245010200041210006900143260000800212300001600220490000600236520208600242100001802328700001702346700001402363700001902377700002102396700002402417700002702441700002302468700001702491856012602508 2016 eng d00aBilayer cryogel wound dressing and skin regeneration grafts for the treatment of acute skinwounds0 aBilayer cryogel wound dressing and skin regeneration grafts for  cJUN a15145-151590 v83 aIn this study, the potential of cryogel bilayer wound dressing and skin regenerating graft for the treatment of surgically created full thickness wounds was evaluated. The top layer was composed of polyvinylpyrrolidone-iodine (PVP-I) and served as the antiseptic layer, while the bottom regenerative layer was made using gelatin cryogel. Both components of the bilayer showed typical features of a cryogel interconnected macropore network, rapid swelling, high water uptake capacity of about 90%. Both PVP and gelatin cryogel showed high tensile strength of 45 and 10 kPa, respectively. Gelatin cryogel sheets were essentially elastic and could be stretched without any visible deformation. The antiseptic PVP-I layer cryogel sheet showed sustained iodine release and suppressed microbial growth when tested with skin pathogens (zone of inhibition cm for sheet of 0.9 cm diameter). The gelatin cryogel sheet degraded in vitro in weeks. The gelatin cryogel sheet supported cell infiltration, attachment, and proliferation of fibroblasts and keratinocytes. Microparticles loaded with bioactive molecules (mannose-6-phosphate and human fibrinogen) were also incorporated in the gelatin cryogel sheets for their role in enhancing skin regeneration and scar free wound healing. In vivo evaluation of healing capacity of the bilayer cryogel was checked in rabbits by creating full thickness wound, defect (diameter 2 cm). Macroscopic and microscopic observation at regular time intervals for 4 weeks demonstrated better and faster skin regeneration in the wound treated with cryogel bilayer as compared to untreated defect and the repair was comparable to commercial skin regeneration scaffold Neuskin-F. Complete skin regeneration was observed after 4 weeks' of implantation with no sign of inflammatory response. Defects implanted with cryogel having mannose-6-phosphate showed no scar formation,, while the wound treated with bilayer incorporated with human fibrinogen microparticles showed early signs of skin regeneration; epidermis formation occurred at 2 weeks after implantation.1 aPriya, Geetha1 aGupta, Ankur1 aJain, Era1 aSarkar, Joyita1 aDamania, Apeksha1 aJagdale, Pankaj, R.1 aChaudhari, Bhushan, P.1 aGupta, Kailash, C.1 aKumar, Ashok uhttps://library.ncl.res.in/content/bilayer-cryogel-wound-dressing-and-skin-regeneration-grafts-treatment-acute-skinwounds01908nas a2200145 4500008004100000245013800041210006900179260000800248300001400256490000600270520130600276100002501582700003001607856012501637 2016 eng d00aBimetallic and plasmonic Ag-Au on TiO2 for solar water splitting : an active nanocomposite for entire visible light region absorption0 aBimetallic and plasmonic AgAu on TiO2 for solar water splitting  cOCT a3294-33010 v83 a

The current study demonstrates a potential method for maximum utilization of the entire visible light region of the solar spectrum by designing a plasmonic-metals semiconductor composite. A combination of Ag+Au nanoclusters would broaden the visible-light absorption at least between 400 and 650nm. This hypothesis was evaluated through solar water splitting (SWS) activity of Au-TiO2, Ag-TiO2, and Ag on Au-TiO2 (AgAuT) composites. AgAuT bimetallic nanocomposite shows the maximum apparent quantum yield of 3.3% with hydrogen generation (718molh.g(-1)) from aqueous methanol, and overall water splitting activity (7molg.h(-1), AQY=0.04%) under one sun conditions. Enhanced photocatalytic activity of AgAuT is partly attributed to the formation of hot electrons. A thin layer of Ag coating on Au particles leads to a core-shell morphology with Au in the core. Fermi level equilibration between metal and titania, and Schottky junction formation are directly demonstrated. The strong electronic interaction between Ag and Au, and with TiO2 is evident from its electron rich character and confirmed by Raman spectroscopy, X-ray photoelectron spectroscopy, photoluminescence, and high-resolution TEM measurements. Electronic factors seem to be responsible for the high rate of hydrogen production.

1 aPatra, Kshirodra, K.1 aGopinath, Chinnakonda, S. uhttps://library.ncl.res.in/content/bimetallic-and-plasmonic-ag-au-tio2-solar-water-splitting-active-nanocomposite-entire02469nas a2200181 4500008004100000022001400041245014300055210006900198260011000267300001600377490000600393520167100399100002102070700002102091700002402112700002102136856013002157 2016 eng d a2046-206900aBioavailability studies of curcumin-sophorolipid nano-conjugates in the aqueous phase: role in the synthesis of uniform gold nanoparticles0 aBioavailability studies of curcuminsophorolipid nanoconjugates i aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJUL a68504-685140 v63 a

The major limiting factors for curcumin to be accepted as a modern drug, despite its widespread applications, are its low aqueous solubility, low retention time and poor bioavailability. When subjected to a mild physical stress, curcumin is observed to internalize within the micellar hydrophobic core of oleic acid sophorolipid resulting in the formation of curcumin-sophorolipid nanoconjugates (CurSL). These bio-composites show enhanced retention time and increased bioavailability of curcumin in rat models. In the presence of gold salts, CurSL act as potent reducing and capping agents, resulting in the synthesis of monodispersed, spherical gold nanoparticles (CurSL-GNPs) of 8-10 nm in size. Physicochemical, morphological and optical characteristics of both the nanoparticles are discussed based on spectroscopic absorption, photoluminescence (PL), dynamic light scattering (DLS), zeta potential, SEM and TEM measurements. FTIR spectroscopy signatures of these nanoparticles confirm the retention of functional groups in the end products. A retention time of 2 hours in blood plasma and an increase in curcumin recovery by about 150 times that previously reported were observed in the pharmacokinetic (pK(a)) studies performed on Wistar rats. The bio-distribution of gold nanoparticles in rats was studied using EDX, which revealed their presence in different vital organs. The absence of unusual legions or necrosis in histopathological analysis of vital organs in all the rat models suggests the use of curcuminsophorolipid nano-conjugates enhances curcumin bioavailability and the Cur-SL based nano-gold formulation is a good drug delivery carrier.

1 aDarne, Priti, A.1 aMehta, Mihir, R.1 aAgawane, Sachin, B.1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/bioavailability-studies-curcumin-sophorolipid-nano-conjugates-aqueous-phase-role-synthesis03390nas a2200553 4500008004100000022001400041245020600055210006900261260011500330300001200445490000700457520135600464653002701820653002901847653002601876653001201902653002801914653002501942653001201967653000701979653002201986653004202008653004902050653004802099653000902147653003202156653006002188653002602248653002102274653002202295653001902317653002702336653001802363653002302381653001702404653001602421653003302437653002202470653003702492653002402529653003702553653001802590653002002608653002202628100001802650700002102668700002602689856012102715 2016 eng d a1751-874100aBiogenic green synthesis of monodispersed gum kondagogu (Cochlospermum gossypium) iron nanocomposite material and its application in germination and growth of mung bean (Vigna radiata) as a plant model0 aBiogenic green synthesis of monodispersed gum kondagogu Cochlosp aMICHAEL FARADAY HOUSE SIX HILLS WAY STEVENAGE, HERTFORD SG1 2AY, ENGLANDbINST ENGINEERING TECHNOLOGY-IETcJUN a141-1460 v103 a

An eco-friendly green and one-pot synthesis of highly monodispersed iron (Fe) nanoparticles (NPs) by using a natural biopolymer, gum kondagogu (GK) as reducing and capping agent is proposed. The NPs synthesised were characterised by ultra-violet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy and X-ray diffraction. As the concentration of gum and time increases, the intensity of NPs formation increased. The NPs were highly monodispersed with uniform circular shapes of 2-6 nm in size. The formed NPs were crystalline in nature which was confirmed by diffraction analysis. The conversion ratio of Fe ionic form to NPs was 21% which was quantified by inductively coupled plasma mass spectroscopy (ICP-MS). Fe is essential for plant growth and development. A study was conducted to examine the effect of these NPs on the growth of mung bean (Vigna radiata). The radical length and biomass was increased in seeds exposed to Fe NPs than the ions. The uptake of Fe NPs by the sprouts was also quantified by ICP-MS, in which Fe was more in mung bean seeds exposed to NPs. The -amylase activity was increased in the seeds exposed to NPs. The observed increase in the biomass by Fe NPs and seed germination may facilitate its application in the agriculture as an important cost-effective method for plant growth.

10aalpha-amylase activity10aBiogenic green synthesis10abiological techniques10aBiomass10aCochlospermum gossypium10adiffraction analysis10aenzymes10aFe10agum concentration10ahigh-monodispersed iron nanoparticles10ainductively coupled plasma mass spectroscopy10aionic-to-nanoparticle iron conversion ratio10airon10amonodispersed gum kondagogu10amonodispersed gum kondagogu iron nanocomposite material10amung bean germination10amung bean growth10aNanobiotechnology10aNanocomposites10ananoparticle formation10aNanoparticles10anatural biopolymer10aplant growth10aplant model10ascanning electron microscopy10asize 2 nm to 6 nm10atransmission electron microscopy10aultraviolet spectra10aultraviolet-visible spectroscopy10aVigna radiata10avisible spectra10aX-ray diffraction1 aRaju, Dugyala1 aMehta, Urmil, J.1 aBeedu, Sashidhar, Rao uhttps://library.ncl.res.in/content/biogenic-green-synthesis-monodispersed-gum-kondagogu-cochlospermum-gossypium-iron02651nas a2200289 4500008004100000022001400041245007800055210006900133260010600202300001200308490000800320520167200328653001702000653001702017653002302034653001702057653002402074100001502098700001702113700002502130700001702155700002402172700001602196700002102212700002202233856010602255 2016 eng d a1386-142500aBiogenic terbium oxide nanoparticles as the vanguard against osteosarcoma0 aBiogenic terbium oxide nanoparticles as the vanguard against ost aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcNOV a123-1310 v1683 a

The synthesis of inner transition metal nanoparticles via an ecofriendly route is quite difficult. This study, for the first time, reports synthesis of terbium oxide nanoparticles using fungus, Fusarium oxysporum. The biocompatible terbium oxide nanoparticles (Tb2O3 NPs) were synthesized by incubating Tb4O7 with the biomass of fungus F. oxysporum. Multiple physical characterization techniques, such as UV-visible and photoluminescence spectroscopy, TEM, SAED, and zeta-potential were used to confirm the synthesis, purity, optical and surface characteristics, crystallinity, size, shape, distribution, and stability of the nanoemulsion of Tb2O3 NPs. The Tb2O3 NPs were found to inhibit the propagation of MG-63 and Saos-2 cell-lines (IC50 value of 0.102 mu g/mL) and remained non-toxic up to a concentration of 0373 gg/mL toward primary osteoblasts. Cell viability decreased in a concentration-dependent manner upon exposure to 10 nmTb(2)O(3) NPs in the concentration range 0.023-0373 mu g/mL Cell toxicity was evaluated by observing changes in cell morphology, cell viability, oxidative stress parameters, and FACS analysis. Morphological examinations of cells revealed cell shrinkage, nuclear condensation, and formation of apoptotic bodies. The level of ROS within the cells an indicator of oxidative stress was significantly increased. The induction of apoptosis at concentrations <= IC50 was corroborated by 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (DNA damage and nuclear fragmentation). Flow-cytometric studies indicated that the response was dose dependent with a threshold effect. (C) 2016 Elsevier B.V. All rights reserved.

10aBiosynthesis10aCytotoxicity10afusarium oxysporum10aOsteosarcoma10aTb2O3 nanoparticles1 aIram, Sana1 aKhan, Salman1 aAnsary, Abu, Ayoobul1 aArshad, Mohd1 aSiddiqui, Sahabjada1 aAhmad, Ejaz1 aKhan, Rizwan, H.1 aKhan, Mohd, Sajid uhttps://library.ncl.res.in/content/biogenic-terbium-oxide-nanoparticles-vanguard-against-osteosarcoma01108nas a2200145 4500008004100000245006100041210006000102260000800162300001400170490000600184520062200190100001400812700001400826856012200840 2016 eng d00aBioinspired eco-friendly synthesis of ZrO2 nanoparticles0 aBioinspired ecofriendly synthesis of ZrO2 nanoparticles cAUG a3068-30750 v73 aHere we report the extracellular biosynthesis of zirconia (ZrO2) nanoparticles under ambient conditions by challenging the fungus Humicola sp. with potassium hexafluorozirconate (K2ZrF6) as a precursor. Transmission electron micrograph (TEM) showed that nanoparticles are quasi-spherical in shape with an average particle size of 13 nm. The structural investigation was done using selected area electron diffraction (SAED) and powder XRD which show that the nanoparticles are perfectly crystalline with an orthorhombic structure. XPS and FTIR analysis explained the presence of proteins on the nanoparticles surfaces.1 aUddin, I.1 aAhmad, A. uhttps://www.scopus.com/record/display.uri?eid=2-s2.0-84988430948&origin=inward&txGid=25fe3c642542aef24895f8428cb2918b01813nas a2200193 4500008004100000022001400041245008400055210006900139260007400208300001000282490000700292520109600299100002301395700001801418700002201436700002101458700002501479856011501504 2016 eng d a0015-563200aBiological activity of sophorolipids and their possible use as antiviral agents0 aBiological activity of sophorolipids and their possible use as a aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJAN a85-890 v613 a

Sophorolipids are surface active glycolipids consisting of a hydrophilic sophorose unit and a hydrophobic portion composed of a fatty acid tail. Crude sophorolipid sample contains both acidic and lactonic forms of sophorolipid with different degrees of acetylation and varying lengths of the fatty acid chains depending on the substrates used in the production process. Carboxylic end in the acidic form of the fatty acid is free, whereas in the lactonic form, it is internally esterified. Sophorolipids show different physicochemical properties with wide range of applications for each structural compound. Lactonic form of sophorolipids shows surface tension reducing ability and biological activity, whereas the acidic form possesses better foam forming ability and higher solubility. Presence of acetyl groups gives hydrophilic nature to the sophorolipids which promotes its antiviral and cytokine-stimulating properties. The aim of this review is to explore and suggest the plausibility of sophorolipids as therapeutic and prophylactic agents for the treatment of viral diseases.

1 aBorsanyiova, Maria1 aPatil, Amrita1 aMukherji, Ruchira1 aPrabhune, Asmita1 aBopegamage, Shubhada uhttps://library.ncl.res.in/content/biological-activity-sophorolipids-and-their-possible-use-antiviral-agents-001352nas a2200157 4500008004100000022001400041245016500055210006900220260007100289300001400360490000700374520065200381100002401033700001901057856011801076 2016 eng d a0022-326300aBiomimetic collective total synthesis of bioactive carbazole alkaloids indizoline, mafaicheenamine A, claulamine A, claulansine A, and the proposed claulamine E0 aBiomimetic collective total synthesis of bioactive carbazole alk a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcJUN a5222-52270 v813 a

The common precursor 1-methoxy-2-prenyl-3-carbomethoxycarbazole was synthesized from dimethyl indolylmethylenesuccinate in four steps. Well-planned reductive and/or oxidative transformations and intramolecular cyclizations were performed on a pivotal common precursor to accomplish collective first total synthesis of titled natural products and proposed Claulamine E. Burgess reagent induced formation of kinetically controlled product Claulamine A, and intramolecular cyclizations to form bicyclic claulansine A were the key reactions. An alternatively attempted synthesis failed to provide the structural isomer of proposed Claulamine E.

1 aMarkad, Shivaji, B.1 aArgade, N., P. uhttps://library.ncl.res.in/content/biomimetic-collective-total-synthesis-bioactive-carbazole-alkaloids-indizoline02328nas a2200193 4500008004100000022001400041245009400055210006900149260011000218300001400328490000600342520154600348100002101894700001901915700002401934700002201958700002701980856012702007 2016 eng d a2040-336400aBioresponsive carbon nano-gated multifunctional mesoporous silica for cancer theranostics0 aBioresponsive carbon nanogated multifunctional mesoporous silica aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcDEC a4537-45460 v83 a

Designing bioresponsive nanocarriers for controlled and efficient intracellular drug release for cancer therapy is a major thrust area in nanomedicine. With recent recognition by the US FDA as a safe material for human trials, mesoporous silica nanoparticles (MSNPs) are being extensively explored as promising theranostic agents. Green fluorescent carbon quantum dots (CQDs), though known as possible alternatives for their more toxic and relatively less efficient predecessors, are less known as gate keepers for drug release control. We report for the first time an efficient bioresponse of CQDs when judiciously designed using glutathione cleavable (redox responsive) disulphide bonds. When the anticancer drug doxorubicin loaded MSNPs are capped with these CQDs, they display promising drug release control on exposure to a mimicked intracellular cancer environment. Their dual functionality is well established with good control on preventing the premature release and exceptional bio-imaging of HeLa cancer cells. Fluorescence images prove selective targeting of HeLa cells by overexpression of folate receptors from the surface functionalised folic acid ligand. Extensive characterisation using XRD, TEM, BET analysis, drug loading tests, drug release kinetics, MTT assay and fluoroscence cell imaging helps in understanding the multi-functionalities of the successful design, extending its scope with exciting prospects towards non-invasive targeted drug delivery and bio-imaging for effective cancer diagnosis and treatment.

1 aPrasad, Rajendra1 aAiyer, Sandhya1 aChauhan, Deepak, S.1 aSrivastava, Rohit1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/bioresponsive-carbon-nano-gated-multifunctional-mesoporous-silica-cancer-theranostics-001991nas a2200229 4500008004100000022001400041245009600055210006900151260009000220300001000310490000600320520113200326100001601458700003101474700001901505700002901524700002301553700002101576700002201597700001801619856012401637 2016 eng d a2045-232200aBiosurfactant as a promoter of methane hydrate formation: thermodynamic and kinetic studies0 aBiosurfactant as a promoter of methane hydrate formation thermod aMACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLANDbNATURE PUBLISHING GROUPcFEB a208930 v63 a

Natural gas hydrates (NGHs) are solid non-stoichiometric compounds often regarded as a next generation energy source. Successful commercialization of NGH is curtailed by lack of efficient and safe technology for generation, dissociation, storage and transportation. The present work studied the influence of environment compatible biosurfactant on gas hydrate formation. Biosurfactant was produced by Pseudomonas aeruginosa strain A11 and was characterized as rhamnolipids. Purified rhamnolipids reduced the surface tension of water from 72 mN/m to 36 mN/m with Critical Micelle Concentration (CMC) of 70 mg/l. Use of 1000 ppm rhamnolipids solution in C type silica gel bed system increased methane hydrate formation rate by 42.97% and reduced the induction time of hydrate formation by 22.63% as compared to water saturated C type silica gel. Presence of rhamnolipids also shifted methane hydrate formation temperature to higher values relative to the system without biosurfactant. Results from thermodynamic and kinetic studies suggest that rhamnolipids can be applied as environment friendly methane hydrate promoter.

1 aArora, Amit1 aCameotra, Swaranjit, Singh1 aKumar, Rajnish1 aBalomajumder, Chandrajit1 aSingh, Anil, Kumar1 aSanthakumari, B.1 aKumar, Pushpendra1 aLaik, Sukumar uhttps://library.ncl.res.in/content/biosurfactant-promoter-methane-hydrate-formation-thermodynamic-and-kinetic-studies-102563nas a2200229 4500008004100000245012400041210006900165260000800234300001400242490000700256520177300263100002602036700001702062700001702079700001502096700001702111700001902128700001702147700002402164700001702188856012802205 2016 eng d00aBiosynthesis of anti-proliferative gold using endophytic fusarium oxysporum strain isolated from neem (A-indica) leaves0 aBiosynthesis of antiproliferative gold using endophytic fusarium cJAN a2036-20420 v163 a

Here we report a simple, rapid, environment friendly approach for the synthesis of gold nanoparticles using neem (Azadirachta indica A. Juss.) fungal endophyte, which based upon morphological and cultural characteristics was eventually identified as Fusarium oxysporum. The aqueous precursor (HAuCl4) solution when reacted with endophytic fungus resulted in the biosynthesis of abundant amounts of well dispersed gold nanoparticles of 10-40 nm with an average size of 22nm. These biosynthesized gold nanoparticles were then characterized by standard analytical techniques such as UV-Visible spectroscopy, X-ray diffraction, Transmission Electron Microscopy and Fourier Transform Infrared Spectroscopy. Cytotoxic activity of these nanoparticles was checked against three different cell types including breast cancer (ZR-75-1), Daudi (Human Burkitt's lymphoma cancer) and normal human peripheral blood mononuclear cells (PBMC), where it was found that our gold nanoparticles are anti-proliferative against cancer cells but completely safe toward normal cells. In addition to this, assessment of toxicity toward human RBC revealed less than 0.1 % hemolysis as compared to Triton X-100 suggesting safe nature of our biosynthesized gold nanoparticles on human cells. Also, our nanoparticles exhibited no anti-fungal (against Aspergillus niger) or anti-bacterial [against Gram positive (Bacillus subtilis & Staphylococcus aureus) and Gram negative (Escherichia coli & Pseudomonas aeruginosa) bacteria] activity thus suggesting their non-toxic, biocompatible nature. The present investigation opens up avenues for eco-friendly, biocompatible nanomaterials to be used in a wide variety of application such as drug delivery, therapeutics, theranostics and so on.

1 aSiddiqui, Ejaz, Ahmad1 aAhmad, Absar1 aJulius, Anju1 aSyed, Asad1 aKhan, Shadab1 aKharat, Mahesh1 aPai, Kalpana1 aKadoo, Narendra, Y.1 aGupta, Vidya uhttps://library.ncl.res.in/content/biosynthesis-anti-proliferative-gold-using-endophytic-fusarium-oxysporum-strain-isolated02606nas a2200181 4500008004100000245012400041210006900165260000800234300001400242490000700256520192700263100001702190700001702207700002602224700002602250700002002276856012802296 2016 eng d00aBiosynthesis of fluorescent Bi2S3 nanoparticles and their application as dual-functionSPECT-CT probe for animal imaging0 aBiosynthesis of fluorescent Bi2S3 nanoparticles and their applic cOCT a2019-20250 v163 aBismuth sulphide (Bi2S3) is an excellent semiconductor and its nanoparticles have numerous significant applications including photovoltaic materials, photodiode arrays, bio-imaging, etc. Nevertheless, these nanoparticles when fabricated by chemical and physical routes tend to easily aggregate in colloidal solutions, are eco-unfriendly, cumbrous and very broad in size distribution. The aim of the present manuscript was to ecologically fabricate water dispersible, safe and stable Bi2S3 nanoparticles such that these may find use in animal imaging, diagnostics, cell labeling and other biomedical applications. Herein, we for the first time have biosynthesized highly fluorescent, natural protein capped Bi2S3 nanoparticles by subjecting the fungus Fusarium oxysporum to bismuth nitrate pentahydrate [Bi(NO3)(3).5H(2)O] alongwith sodium sulphite (Na2SO3) as precursor salts under ambient conditions of temperature, pressure and pH. The nanoparticles were completely characterized using recognized standard techniques. These natural protein capped Bi2S3 nanoparticles are quasi-spherical in shape with an average particle size of 15 nm, maintain long term stability and show semiconductor behavior having blue shift with a band gap of 3.04 eV. Semiconductor nanocrystals are fundamentally much more fluorescent than the toxic fluorescent chemical compounds (fluorophores) which are presently largely employed in imaging, immunohistochemistry, biochemistry, etc. Biologically fabricated fluorescent nanoparticles may replace organic fluorophores and aid in rapid development of biomedical nanotechnology. Thus, biodistribution study of the so-formed Bi2S3 nanoparticles in male Sprague Dawley rats was done by radiolabelling with Technitium-99m (Tc-99m) and clearance time from blood was calculated. The nanoparticles were then employed in SPECT-CT probe for animal imaging where these imparted iodine equivalent contrast.1 aUddin, Imran1 aAhmad, Absar1 aSiddiqui, Ejaz, Ahmad1 aRahaman, Sk., Hasanur1 aGambhir, Sanjay uhttps://library.ncl.res.in/content/biosynthesis-fluorescent-bi2s3-nanoparticles-and-their-application-dual-functionspect-ct03132nas a2200181 4500008004100000022001400041245011800055210006900173260011000242300001600352490000600368520237100374100001902745700001702764700001802781700001902799856013202818 2016 eng d a2046-206900aBroad spectrum photon responsive, paramagnetic beta-NaGdF4: Yb3+, Er3+ - mesoporous anatase titania nanocomposite0 aBroad spectrum photon responsive paramagnetic betaNaGdF4 Yb3 Er3 aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcMAY a53504-535180 v63 a

Herein, we report a novel single multifunctional platform based on broad-spectrum photoactive beta-NaGdF4:18% Yb3+, 2% Er3+ and mesoporous anatase TiO2 for enhanced energy and simultaneous biomedical applications. Currently, the photoactive materials for solar energy harvesting applications have limitations in their efficiency due to their narrow photon absorption spectrum. The upconversion phosphor beta-NaGdF4: 18% Yb3+, 2% Er3+ nanorods collect and harvest the NIR photons (similar to 980 nm) of sunlight and transform them into visible light via anti-Stokes emission (lambda(em) similar to 521 and similar to 540 nm), and the photoactive mesoporous anatase TiO2 (mTiO(2)) utilizes UV and weak visible photons, thus the composite forms a broad spectrum photon-capture system and improved power conversion efficiency for enhanced applications in photocatalysis, and dye sensitized solar cells (DSSCs). The photocatalytic activity of the nanocomposite showed an improvement in comparison to the mTiO(2) for the degradation of various dyes. In addition, the photocurrent density and solar cell efficiency of the nanocomposites showed an improvement by similar to 24% and similar to 17% respectively, over mTiO(2). The beta-NaGdF4: Yb3+, Er3+/mTiO(2) nanocomposite exhibits a strong paramagnetic signal (chi similar to 6.45 x 10(-5) emu g(-1) Oe(-1)). The nuclear magnetic resonance (NMR) measurements showed large longitudinal T1 relaxivity (r(1) = 7.09 s(-1) mM(-1)) and magnetic resonance imaging showed enhanced T-1-weighted MRI images with increased concentrations of beta-NaGdF4: Yb3+, Er3+/mTiO(2) nanocomposite making them suitable for simultaneous magnetoresonance imaging. In addition, this composite system can also be used as a NIR triggered drug delivery system and in biomedical applications. Moreover, mesoporous TiO2 is expected to increase the photocatalytic active sites, dye, and absorption, and drug loading capacity. The as-designed multifunctional beta-NaGdF4:Yb3+, Er3+/mTiO(2) nanocomposite possessed simultaneous multiple discrete functionalities with excellent luminescence properties, intrinsic paramagnetism, biocompatibility, improved photocatalytic activity, and solar cell efficiency. This work provides a promising system to utilize NIR light, which will contribute to efficient photon harvesting and biological applications.

1 aPadhye, Preeti1 aSadhu, Subha1 aMalik, Monika1 aPoddar, Pankaj uhttps://library.ncl.res.in/content/broad-spectrum-photon-responsive-paramagnetic-beta-nagdf4-yb3-er3-mesoporous-anatase-titania01793nas a2200145 4500008004100000245012800041210006900169260000800238300001600246490000600262520120200268100001701470700003001487856013001517 2016 eng d00aBromide ion mediated modification to digestive ripening process: preparation of ultra-small Pd, Pt, Rh and Ru nanoparticles0 aBromide ion mediated modification to digestive ripening process  cMAY a2007–20170 v93 a

Nanoparticles of catalytically important transition metals, such as Pd, Pt, Rh, and Ru have been prepared by the well-known “digestive ripening” (DR) and “modified digestive ripening” (mDR) methods. In the traditional DR process, a polydisperse colloidal dispersion is refluxed in the presence of a surface-active molecule, such as alkanethiol. The mDR method involved a small modification in the procedure, wherein refluxing was performed with an alkanethiol and a tetra-alkylammonium bromide surfactant. This minor modification led to a dramatic change in the final particle size distributions, giving access to nanoparticles in the <3 nm size regime; this was not possible with the traditional DR process. Bromide ions, which are present during refluxing, proved to be an important ingredient in the modification process. These bromide ions are revealed to act as etchants, resulting in ultra-small nanoparticles. All transition metal nanoparticles investigated displayed catalytic activity in the reduction reaction of p-nitro phenol. Pd nanoparticles, synthesized by a modified digestive ripening method, exhibited the best catalytic activity among the systems investigated.

1 aSeth, Jhumur1 aPrasad, Bhagavatula L. V. uhttps://library.ncl.res.in/content/bromide-ion-mediated-modification-digestive-ripening-process-preparation-ultra-small-pd-pt01968nas a2200253 4500008004100000022001400041245017900055210006900234260007500303300001400378520096100392653001601353653002301369653001601392653002301408653002601431100001901457700002501476700001801501700001801519700002201537700002401559856013101583 2016 eng d a1434-193X00aBronsted-acid-mediated divergent reactions of betti bases with indoles: an approach to chromeno[2,3-b]indoles through intramolecular dehydrogenative C2-alkoxylation of indole0 aBronstedacidmediated divergent reactions of betti bases with ind aPOSTFACH 101161, 69451 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcJUL a3441-34483 a

Divergent reactions of various 1-(aminoalkyl) naphthols and 2-(aminoalkyl) phenols (commonly known as Betti bases) with indoles under Bronsted acid catalysis is reported. With the reaction strategies, one can efficiently synthesize important indole derivatives such as 3-(alpha,alpha-diarylmethyl)indoles and chromeno[2,3-b]indoles. Furthermore, we disclose here a new C-C bond-cleavage reaction, in which naphthol and phenol behave as leaving groups to produce diarylmethanes. Inexpensive reagents such as p-toluenesulfonic acid monohydrate and molecular iodine are used to catalyze the reactions. No metal catalyst is required. The starting material of the reactions, Betti bases, are easily prepared from a three-component reaction of naphthol/phenol, aldehydes, and secondary amines. The mechanisms for the reactions are established through some control experiments. Quinone methide is the key intermediate for all the reactions reported herein.

10aBetti bases10aC-O bond formation10aDearylation10aFused-ring systems10anitrogen heterocycles1 aDeb, Mohit, L.1 aPegu, Choitanya, Dev1 aDeka, Bhaskar1 aDutta, Prantu1 aKotmale, Amol, S.1 aBaruah, Pranjal, K. uhttps://library.ncl.res.in/content/bronsted-acid-mediated-divergent-reactions-betti-bases-indoles-approach-chromeno23-bindoles02290nas a2200205 4500008004100000245011900041210006900160260000800229300001200237490000800249520153200257100002101789700002401810700002701834700002201861700002401883700002501907700002001932856013201952 2016 eng d00aBudding trends in integrated pest management using advanced micro- and nano-materials: challenges and perspectives0 aBudding trends in integrated pest management using advanced micr cDEC a157-1690 v1843 aOne of the most vital supports to sustain human life on the planet earth is the agriculture system that has been constantly challenged in terms of yield. Crop losses due to insect pest attack even after excessive use of chemical pesticides, are major concerns for humanity and environment protection. By the virtue of unique properties possessed by micro and nano-structures, their implementation in Agri-biotechnology is largely anticipated. Hence, traditional pest management strategies are now forestalling the potential of micro and nanotechnology as an effective and viable approach to alleviate problems pertaining to pest control. These technological innovations hold promise to contribute enhanced productivity by providing novel agrochemical agents and delivery systems. Application of these systems engages to achieve: i) control release of agrochemicals, ii) site-targeted delivery of active ingredients to manage specific pests, iii) reduced pesticide use, iv) detection of chemical residues, v) pesticide degradation, vi) nucleic acid delivery and vii) to mitigate post-harvest damage. Applications of micro and nano-technology are still marginal owing to the perception of low economic returns, stringent regulatory issues involving safety assessment and public awareness over their uses. In this review, we highlight the potential application of micro and nano-materials with a major focus on effective pest management strategies including safe handling of pesticides. (C) 2016 Elsevier Ltd. All rights reserved.1 aKhandelwal, Neha1 aBarbole, Ranjit, S.1 aBanerjee, Shashwat, S.1 aChate, Govind, P.1 aBiradar, Ankush, V.1 aKhandare, Jayant, J.1 aGiri, Ashok, P. uhttps://library.ncl.res.in/content/budding-trends-integrated-pest-management-using-advanced-micro-and-nano-materials-challenges02122nas a2200193 4500008004100000245016100041210006900202260000800271300001000279490000700289520137600296100001501672700001801687700001501705700003301720700001901753700002601772856013001798 2016 eng d00aBuilding and analysis of protein-protein interactions related to diabetes mellitus using support vector machine, biomedical text mining and network analysis0 aBuilding and analysis of proteinprotein interactions related to  cDEC a37-440 v653 aIn order to understand the molecular mechanism underlying any disease, knowledge about the interacting proteins in the disease pathway is essential. The number of revealed protein-protein interactions (PPI) is still very limited compared to the available protein sequences of different organisms. Experiment based high-throughput technologies though provide some data about these interactions, those are often fairly noisy. Computational techniques for predicting protein protein interactions therefore assume significance. 1296 binary fingerprints that encode a combination of structural and geometric properties were developed using the crystallographic data of 15,000 protein complexes in the pdb server. In a case study, these fingerprints were created for proteins implicated in the Type 2 diabetes mellitus disease. The fingerprints were input into a SVM based model for discriminating disease proteins from non disease proteins yielding a classification accuracy of 78.2% (AUC value of 0.78) on an external data set composed of proteins retrieved via text mining of diabetes related literature. A PPI network was constructed and analysed to explore new disease targets. The integrated approach exemplified here has a potential for identifying disease related proteins, functional annotation and other proteomics studies. (C) 2016 Elsevier Ltd. All rights reserved.1 aVyas, Renu1 aBapat, Sanket1 aJain, Esha1 aKarthikeyan, Muthukumarasamy1 aTambe, Sanjeev1 aKulkarni, Bhaskar, D. uhttps://library.ncl.res.in/content/building-and-analysis-protein-protein-interactions-related-diabetes-mellitus-using-support02432nas a2200265 4500008004100000245014900041210006900190260000800259300000900267490000800276520151200284653002801796653002401824653001401848653001901862653002201881653002201903100002001925700001701945700001701962700001601979700003001995700001802025856012302043 2017 eng d00aBand alignment and charge transfer pathway in three phase anatase-rutile-brookite TiO2 nanotubes: an efficient photocatalyst for water splitting0 aBand alignment and charge transfer pathway in three phase anatas cDEC a9-190 v1283 aThe study reports electrochemical synthesis, phase evolution and hydrogen generation efficiency of anatase, anatase-rutile and anatase-rutile-brookite (ARB) TiO2 nanotubes for the first time. The SEM and TEM micrographs confirm the tubular morphology of the samples. The presence of anatase, rutile and brookite phases in a single nanotube is confirmed from high resolution TEM analysis. The water splitting efficiency of the three systems are studied under one sun illumination. It is observed that the anatase-rutile-brookite TiO2 nanotubes are highly efficient compared to anatase-rutile or anatase TiO2 nanotubes. The hydrogen generated by ARB composites, after four hours of one sun illumination, is found to be nearly twice that of anatase TiO2 nanotubes and 1.6 times that of anatase-rutile TiO2 nanotubes. The results suggest that the ARB in single nanotube having two junction interfaces, highly facilitate interparticle charge transfer compared to single junction anatase-rutile or bare anatase TiO2 nanotubes. From the deconvolution of PL spectra and the synchrotron radiation assisted valence band edge analysis, the band diagram for the anatase-rutile-brookite phase is constructed. The charge separation and its transfer pathway for efficient photo-assisted water splitting are delineated. This opens a new route for the simple synthesis and study of tri-phase TiO2 for efficient photocatalytic water splitting compared to the widely studied two phase TiO2. (C) 2017 Published by Elsevier B.V.10aAnatase-Rutile-Brookite10aHydrogen generation10ananotubes10aPhotocatalysis10aphotoluminescence10aValence band edge1 aPreethi, L., K.1 aMathews, Tom1 aNand, Mangla1 aJha, S., N.1 aChinnakonda, Gopinath, S.1 aDash, Sitaram uhttps://library.ncl.res.in/content/band-alignment-and-charge-transfer-pathway-three-phase-anatase-rutile-brookite-tio201131nas a2200157 4500008004100000245013200041210006900173260000800242490000700250520050200257100002200759700002300781700002400804700002200828856012300850 2017 eng d00aBase-free and catalyst-free synthesis of functionalized dihydrobenzoxazoles via vinylogous carbonate to carbamate rearrangement0 aBasefree and catalystfree synthesis of functionalized dihydroben cOCT0 v193 aAn unexpected, catalyst-free, and base-free intramolecular cyclization of N-aryloxyacrylate aldimines, under thermal conditions leading to the synthesis of functionalized dihydrobenzoxazoles, is reported. The reaction features a unique rearrangement of vinylogous carbonates to vinylogous carbamates resulting in a new carbon–oxygen and carbon–nitrogen bond construction. The reaction tolerates a broad range of functional groups and the desired products are formed in moderate to good yields.1 aDas, Tamal, Kanti1 aMondal, Santigopal1 aGonnade, Rajesh, G.1 aBiju, Akkattu, T. uhttps://library.ncl.res.in/content/base-free-and-catalyst-free-synthesis-functionalized-dihydrobenzoxazoles-vinylogous00902nas a2200157 4500008004100000245012400041210006900165260000800234300001400242490000700256520031000263100001400573700001400587700001600601856012700617 2017 eng d00aBenz-amidinato calcium iodide catalyzed aldehyde and ketone hydroboration with unprecedented functional group tolerance0 aBenzamidinato calcium iodide catalyzed aldehyde and ketone hydro cAPR a4562-45640 v533 aA benz-amidinato calcium compound, [PhC(NiPr)(2)Cal] (1), catalyzed hydroboration of a wide range of aldehydes and ketones using pinacolborane (HBpin) at room temperature is reported. The catalyst shows functional group tolerance even towards OH and NH groups. The strategy was further extended to imines.1 aYadav, S.1 aPahar, S.1 aSen, S., S. uhttps://library.ncl.res.in/content/benz-amidinato-calcium-iodide-catalyzed-aldehyde-and-ketone-hydroboration-unprecedented02921nas a2200409 4500008004100000022001400041245010200055210006900157260000800226490000900234520164900243653004501892653001801937653002101955653001601976653002301992653001102015653001602026653000802042653003002050653002102080653001902101653001902120653001602139653001202155100001602167700002202183700002002205700002602225700002702251700002002278700001702298700001802315700002102333700002702354856013002381 2017 eng d a0022-286000aBinding selectivity of vitamin K3 based chemosensors towards nickel(II) and copper(II) metal ions0 aBinding selectivity of vitamin K3 based chemosensors towards nic cSEP0 v11433 a

The vitamin K3 derivatives 2-methyl-3-[(pyridin-2-ylmethyl)-amino]-1,4-naphthoquinone (M-1), 2-methyl-3-[(pyridin-2-ylethyl)-amino]-1,4-naphthoquinone (M-2), 2-methyl-3-((2-(thiophen-2-yl) methyl)amino)naphthalene-1,4-dione (M-3) and 2-methyl-3-((2-(thiophen-2-yl)ethyl)amino)naphthalene-1,4-dione (M-4) have been synthesized, characterized and studied for their chemosensor abilities towards transition metal ions. Crystal structures of M-1 to M-4 revealed a variety of N-H center dot center dot O, C-H center dot center dot center dot O, C-H center dot center dot center dot pi and pi center dot center dot center dot pi interactions. Minor variations in such interactions by chemical stimuli such as metal ions, results in change in color that can be visualized by naked eyes. It has been shown that electronic structure and H-1 NMR, vibrational as well as electronic spectra from the density functional theory agree well with the experiments. The metal ion binding in ethanol, ethanol-water and in mild base triethylamine brings forth recognizing ability of M-1 toward Ni2+ whereas M-2 exhibits large sensing ability for Cu2+ ion. Interestingly M-1 display varying metal ion binding specificity in different solvents with the association constant in ethanol being 11,786 M-1 for Ni2+ compared to 9462 M-1 for the Cu2+. A reversal in preferential binding of M-2 with the respective association constants being 4190 M-1 and 6370 M-1 is discernible. (C) 2017 Elsevier B.V. All rights reserved.

10aAminonaphthoquinone Molecular-structures10aAnthraquinone10aAqueous-solution10aChemosensor10aCrystal-structures10aCu(II)10aDerivatives10aDFT10aFluorometric Chemosensors10ahydrogen bonding10aNaphthoquinone10api-pi stacking10aRecognition10asensors1 aPatil, Amit1 aLande, Dipali, N.1 aNalkar, Archana1 aGejji, Shridhar, P. (1 aChakrovorty, Debamitra1 aGonnade, Rajesh1 aMoniz, Tania1 aRangel, Maria1 aPereira, Eulalia1 aSalunke-Gawali, Sunita uhttps://library.ncl.res.in/content/binding-selectivity-vitamin-k3-based-chemosensors-towards-nickelii-and-copperii-metal-ions01435nas a2200181 4500008004100000245008000041210006900121260000800190300001200198490000700210520081100217100002401028700001601052700002401068700002801092700002201120856011101142 2017 eng d00aBiocompatible superhydrophobic coating material for biomedical applications0 aBiocompatible superhydrophobic coating material for biomedical a cMAR a791-7960 v813 aBiomaterials and wettability have played a crucial role in the biocompatibility with a host matrix of body fluid and cells. We discuss designing superhydrophobic biomaterials for novel applications such as temporally implant, contact lenses, controlled drug release coatings, coating on medical instruments, etc. Such Superhydrophobic coating surfaces were created with the simple dip coating method by single step base catalyst sol-gel method. Methyltriethoxysilane/trimethoxymethylsilane-based deposition at different dip periods introduces interesting properties in the region, including superhydrophobicity, biocompatibility and transparency. This works reveal the impact of interaction bone marrow mesenchymal stem cells and surface free energy on the biocompatibility of superhydrophobic biomaterial.1 aMahadik, Satish, A.1 aPedraza, F.1 aMahadik, Sarika, S.1 aRelekar, Brahmanand, P.1 aThorat, Sagar, S. uhttps://library.ncl.res.in/content/biocompatible-superhydrophobic-coating-material-biomedical-applications02308nas a2200157 4500008004100000245015100041210006900192260000800261300001200269490000800281520165500289100002601944700002601970700002301996856013102019 2017 eng d00aBiology and development of xanthopimpla pedator (hymenoptera: ichneumonidae): pupal endoparasitoid of antheraea mylitta (Lepidoptera: Saturniidae)0 aBiology and development of xanthopimpla pedator hymenoptera ichn cNOV a544-5500 v1103 aThe Antheraea mylitta (Drury) (Lepidoptera: Saturniidae) is a commercially important species for the production of tasar silk. However, the Xanthopimpla pedator (F.) (Hymenoptera: Ichneumonidae) is a major pupal endoparasitoid of A. mylitta, which affects the total tasar silk production in India. A gravid female X. pedator parasitizes the pupae of A. mylitta by depositing an egg, which produces a single offspring per host. We studied the development of immature forms of X. pedator by dissecting parasitized pupae of A. mylitta under laboratory conditions at 28-30 degrees C and 40-60% relative humidity. All immature stages of the parasitoid develop within the host in 20-22 d, by devouring the entire pupal content, resulting in dead or seedless cocoons. The total life cycle of X. pedator is completed inside the host pupa and the adult emerges out by rupturing the peduncle end of the cocoon. During development of X. pedator, five preimaginal stages, viz., egg, larval instars, prepupa, pupa, and a pharate adult, were observed. Xanthopimpla pedator passes through five larval instars identified by structural and morphometric variations. These include primary larva (L1), the intermediate larvae (L2-L4), and the finalinstar larva (L5). Biometric study and larval head width permitted the morphological separation of all five larval instars. In the present study, the biology and development of X. pedator inside the pupa of A. mylitta are reported for the first time with detailed photomicrographs. Therefore, the study may enable the information concerning physiological interactions between A. mylitta and its endoparasitoid, X. pedator.1 aGathalkar, Ganesh, B.1 aBarsagade, Deepak, D.1 aSen, Avalokiteswar uhttps://library.ncl.res.in/content/biology-and-development-xanthopimpla-pedator-hymenoptera-ichneumonidae-pupal-endoparasitoid00598nas a2200109 4500008004100000245010700041210006900148260009200217100003300309700001500342856013100357 2017 eng d00aBiomedical literature mining for protein-protein interactions analysis using electronic mailing system0 aBiomedical literature mining for proteinprotein interactions ana a San Francisco, CAbAmer chemical soc, 1155 16TH ST, NW, Washington, DC 20036 USAcAPR 1 aKarthikeyan, Muthukumarasamy1 aVyas, Renu uhttps://library.ncl.res.in/content/biomedical-literature-mining-protein-protein-interactions-analysis-using-electronic-mailing01737nas a2200145 4500008004100000245008600041210006900127260000800196300001400204520119500218100002501413700001701438700002301455856011301478 2017 eng d00aBiomimetic synthesis of CdSe nanoparticles with potential bioimaging applications0 aBiomimetic synthesis of CdSe nanoparticles with potential bioima cJUN a2526-25323 aThis study presents in vitro biomimetic synthesis of CdSe nanoparticles using the enzyme, NADPH dependent-Nitrate reductase purified from fungus, Fusarium oxysporum. The synthesis of CdSe nanoparticles was accomplished in the presence of a synthetic peptide having amino acid sequence (γ-Glu-Cys-Glu-Cys)-Gly, which acted as binding molecule. The nanoparticles were synthesized in the size range of 3.9–9.0 nm was analysed using TEM. Further characterization of nanoparticles was done using techniques such as UV, PL, XPS, and FTIR. The as-synthesized CdSe nanoparticles were water dispersible containing free reactive amino groups. These CdSe nanoparticles were conjugated with bi-antennary and tri-antennary glycopeptides opening up the possibilities for their applications in bioimaging. These significant observations will help better understand the mechanism of biosynthesis of fluorescent nanoparticles using fungi. These findings promise an alternative strategy for an eco-friendly, economical, and large-scale synthesis of water dispersible CdSe nanoparticles. These CdSe nanoparticles have promising biomedical-imaging applications without a need for further functionalization.1 aAnsary, Abu, Ayoobul1 aUddin, Imran1 aKhan, Muhammed, I. uhttps://library.ncl.res.in/content/biomimetic-synthesis-cdse-nanoparticles-potential-bioimaging-applications01186nas a2200157 4500008004100000245009000041210006900131260000800200300001400208490000700222520059700229100002800826700002400854700002800878856012200906 2017 eng d00aBiomimetic total synthesis of angiopterlactone B and other potential natural products0 aBiomimetic total synthesis of angiopterlactone B and other poten cJUL a3564-35670 v193 aA one-pot biomimetic synthesis of (-)-angiopterlactone B and its enantiomer (+)-angiopterlactone B has been accomplished via TBAF-catalyzed tandem ring contraction followed by oxa-Michael/Michael addition sequence. Comparison of specific optical rotations, absolute configurations, and CD spectra of natural, synthesized (-)-angiopterlactone B and (+)-angiopterlactone B unequivocally proves that the isolated angiopterlactone B must be levorotatory. Synthesis of hitherto undiscovered natural products 18 and 20 and analogues of angiopterlactone B demonstrate the versatility of this method.1 aKotammagari, Tharun, K.1 aGonnade, Rajesh, G.1 aBhattacharya, Asish, K. uhttps://library.ncl.res.in/content/biomimetic-total-synthesis-angiopterlactone-b-and-other-potential-natural-products02087nas a2200229 4500008004100000245008000041210006900121260000800190300001400198490000800212520133400220100002601554700002001580700002101600700001701621700001601638700002501654700002601679700002701705700001901732856010601751 2017 eng d00aBiosurfactant surfactin as a kinetic promoter for methane hydrate formation0 aBiosurfactant surfactin as a kinetic promoter for methane hydrat cMAY a5011-50170 v1053 aIn the present study, the effect of the biosurfactant Surfactin on methane hydrate formation kinetics was studied. Initially, several marine derived species were screened for the presence of Surfactin. The polymerase chain reaction technique was used as the preliminary screening step for Surfactin which was then followed up by a couple of different assays to provide conclusive evidence of the same. Based on these tests, the D-9 bacterial strain was identified as a producer of Surfactin. Once the presence of Surfactin had been proven, its effect on methane hydrate formation kinetics was investigated upon by carrying out hydrate formation experiments in a stirred tank reactor. The cell free supernatant containing Surfactin was itself used as the hydrate forming solution without any further processing. It was found that the presence of Surfactin in the system greatly enhances hydrate formation kinetics as compared to pure water. In fact the kinetics in presence of Surfactin also surpassed that obtained with 1 wt% SDS, the most commonly used synthetic kinetic hydrate promoter. This basic study can pave the way for more sophisticated research on the use of biosurfactants as kinetic promoters with a view on rapid methane hydrate formation kinetics for applications such as methane separation, storage and transport.1 aBhattacharjee, Gaurav1 aBarmecha, Vivek1 aPradhan, Darshan1 aNaik, Rajesh1 aZare, Kirti1 aMawlankar, Rahul, R.1 aDastager, Syed, Gulam1 aKushwaha, Omkar, Singh1 aKumar, Rajnish uhttps://library.ncl.res.in/content/biosurfactant-surfactin-kinetic-promoter-methane-hydrate-formation02026nas a2200169 4500008004100000245009900041210006900140260000800209300001400217490000900231520140500240100001801645700002501663700002201688700002101710856012501731 2017 eng d00aBiosynthesis of glycomonoterpenes to attenuate quorum sensing associated virulence in bacteria0 aBiosynthesis of glycomonoterpenes to attenuate quorum sensing as cAPR a1533-15480 v181 3 aThe acquisition of multidrug resistance in bacteria has become a bigger threat of late, mainly due to the bacterial signaling phenomenon, quorum sensing (QS). QS, among a population of bacteria, initiates the formation of biofilms and offers myriad advantages to bacteria. Burgeoning antibiotic resistance in biofilm-producing bacteria has motivated efforts toward finding new alternatives to these traditional antimicrobials. In the present study, we report the increased solubility and additional quorum quenching as well as biofilm disruption activity of glyco-derivatives of monoterpenes (citral and citronellal). Glycomonoterpenes of citral and citronellal were synthesized via conjugation of the monoterpenes with glucose by the non-pathogenic yeast Candida bombicola (ATCC 22214). Structural elucidation of newly synthesized glycomonoterpenes showed that one synthesized using citronellal contains three major lactonic forms with molecular weight 492.43, 473.47, and 330.39 Da whereas the one produced using citral has an acidic form with molecular weight 389.33 and 346.23 Da. The glycomonoterpenes were able to individually inhibit QS, mediated through various medium-chain and long-chain N-acyl homoserine lactones (AHLs). These new compounds are interesting additions to the known range of quorum sensing inhibitors (QSIs) and could be further explored for potential clinical applications.1 aPatil, Amrita1 aJoshi-Navre, Kasturi1 aMukherji, Ruchira1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/biosynthesis-glycomonoterpenes-attenuate-quorum-sensing-associated-virulence-bacteria04502nas a2200169 4500008004100000020002000041245014000061210006900201260005200270300001200322520380200334100001404136700001704150700002004167700001904187856012604206 2017 eng d a978-331951619-600aBiotechnological interventions to modulate terpenoid indole alkaloid pathway in catharanthus roseus using in vitro tools and approaches0 aBiotechnological interventions to modulate terpenoid indole alka a SwitzerlandbSpringer International Publishing a247-2753 a

Catharanthus roseus plant is valued for harboring more than 130 bioactive terpenoid indole alkaloids (TIAs) including the two of its leaf derived bisindole alkaloids-vinblastine and vincristine which are indispensible constituents of antineoplastic drugs used in metastatic malignancy associated with acute lymphoblastic leukaemia's and Hodgkin's/Non-Hodgkin's lymphomas. The extremely low in planta occurrence of TIAs in C. roseus plants resulting in high commercial demand and exorbitant price have brought this herb in focus of an intense scientific scrutiny in last 30 years. Research efforts have so far advanced in two major directions: towards understanding the enzymology and genetic regulation of the concerned metabolic pathway(s) leading to TIAs biosynthesis in plant and, secondly, exploring the possibility of developing cell/tissue culture based platforms for in vitro TIAs production to meet the industry's demand. Designing plants, free from such metabolic constraints, can be a possible approach to enhance the production of plant based medicines. This subject of plant metabolic engineering is gaining lot of attention these days. Pathway manipulation using the modern tools of genetic engineering to over-express a limiting enzyme or to suppress the expression of an enzyme using a shared substrate of a branched pathway are attractive options of metabolic engineering for diverting the metabolic flux towards the synthesis of a desired end product. Knowledge, thus gained, indicates that TIAs biogenetic route is characterized by extensive metabolic cross-talk and shuttling of at least 35 intermediates synthesized via 30 enzymatic reactions occurring in four different types of tissues (epidermis, internal phloem parenchyma, idioblasts and leticifers) and five different sub-cellular compartments (cytosol, vacuole, thyllakoid membrane, nucleus and endoplasmic reticulum). The complexity is further compounded by extremely high level of recalcitrancy of C. roseus plant for regeneration and Agrobacterium- mediated genetic transformation for pathway engineering. As a consequence, all genetic modulation efforts so far made in C. roseus are confined to cell suspension and transformed hairy root cultures that lack the required level of cyto- and tissue- differentiation essential for the expression of entire TIAs pathway genes and enzymes. A perusal of published work in C. roseus clearly suggests that inspite of several pathway manipulation/engineering attempts, the level of TIAs production in cell/tissue/hairy root cultures of this herb could never be enhanced to the level of expectations. The enzymatic, developmental and environmental rigidity/complexi- ties associated with the biosynthetic pathway of these alkaloids have often been cited as possible reasons for these disappointing outcomes Therefore, three major areas of investigation are in focused attention of Catharanthus researchers' the world over are: (1) how to select or design the starting cells or tissue(s) to realize the full potential of applying metabolic engineering tools for up-regulating the TIAs pathway in them; (2) how to overcome the strong recalcitrancy of Catharanthus plant tissues for de novo organogenesis and in vitro plant regeneration for whole plant-level expression of a transgene coding either for a limiting pathway enzyme or a transcription factor that can control the global expression of several pathway genes and, (3) how to overcome the inability of non-differentiated cell cultures to execute those pathway steps that are expressed only in specialized tissues/cells of C. roseus plants. Various biotechnological approaches and generation of novel tissue types have been discussed in the present chapter for the modulation and increased TIAs flux in C. Roseus.

1 aVerma, P.1 aKhan, S., A.1 aParasharami, V.1 aMathur, A., K. uhttps://library.ncl.res.in/content/biotechnological-interventions-modulate-terpenoid-indole-alkaloid-pathway-catharanthus02038nas a2200169 4500008004100000245015500041210006900196260000800265300001000273490000700283520134800290100002901638700002701667700002201694700002001716856013201736 2017 eng d00aBiotransformation of penicillin V to 6-aminopenicillanic acid using immobilized whole cells of E. coli expressing a highly active penicillin V acylase0 aBiotransformation of penicillin V to 6aminopenicillanic acid usi cJAN a52-570 v473 aThe production of 6-aminopenicillanic acid (6-APA) is a key step in the manufacture of semisynthetic antibiotics in the pharmaceutical industry. The penicillin G acylase from Escherichia coli has long been utilized for this purpose. However, the use of penicillin V acylases (PVA) presents some advantages including better stability and higher conversion rates. The industrial application of PVAs has so far been limited due to the nonavailability of suitable bacterial strains and cost issues. In this study, whole-cell immobilization of a recombinant PVA enzyme from Pectobacterium atrosepticum expressed in E. coli was performed. Membrane permeabilization with detergent was used to enhance the cell-bound PVA activity, and the cells were encapsulated in calcium alginate beads and cross-linked with glutaraldehyde. Optimization of parameters for the biotransformation by immobilized cells showed that full conversion of pen V to 6-APA could be achieved within 1 hr at pH 5.0 and 35 degrees C, till 4% (w/v) concentration of the substrate. The beads could be stored for 28 days at 4 degrees C with minimal loss in activity and were reusable up to 10 cycles with 1-hr hardening in CaCl2 between each cycle. The high enzyme productivity of the PVA enzyme system makes a promising case for its application for 6-APA production in the industry.1 aAvinash, Vellore, Sunder1 aChauhan, Palna, Dinesh1 aGaikwad, Shraddha1 aPundle, Archana uhttps://library.ncl.res.in/content/biotransformation-penicillin-v-6-aminopenicillanic-acid-using-immobilized-whole-cells-e-coli01515nas a2200157 4500008004100000245006600041210006600107260000800173300001400181490000700195520098700202100002701189700002201216700001801238856010101256 2017 eng d00aBronsted acid catalyzed enantioselective pericyclic reactions0 aBronsted acid catalyzed enantioselective pericyclic reactions cJUL a4023-40380 v733 aPericyclic reactions can be challenging processes to render asymmetric, due to the concerted and non polar nature of the transition states. Several examples have been reported in which a suitable catalyst binds to a heteroatom-containing substituent in the substrate and accelerates the reaction rate. The requirement of coordinating functionalized substrates has limited the generality of such asymmetric transformations. Multifunctional Bronsted acids are promising catalysts that represent a new paradigm in asymmetric pericyclic reactions. These chiral catalysts rely on multiple non-covalent interactions in the transition state for asymmetric induction, which has been utilized to activate more general substrate classes. In this review, we will cover recent advances in Bronsted acid catalyzed pericyclic reactions and discuss the impact of catalyst design on the expansion of substrate scope for highly enantioselective processes. (C) 2017 Elsevier Ltd. All rights reserved.1 aSleet, Christopher, E.1 aTambar, Uttam, K.1 aMaity, Pradip uhttps://library.ncl.res.in/content/bronsted-acid-catalyzed-enantioselective-pericyclic-reactions01167nas a2200241 4500008004100000022001500041245009200056210006900148260000800217300001400225490000700239520040200246100001700648700002100665700001900686700001900705700001900724700001600743700001600759700001600775700001600791856011800807 2018 eng d a 0887-624X00a Balancing antimicrobial performance with hemocompatibility in amphiphilic homopolymers0 aBalancing antimicrobial performance with hemocompatibility in am cNOV a2391-23960 v563 aThis work establishes amphiphilic polymethacrylates as new antimicrobial candidates that can be synthesized through a combination of controlled free radical polymerization and sequential postpolymerization modification protocols. When conjugated with a polyethylene glycol segment, the block copolymers exhibit a balanced performance between high antibacterial activity and high hemocompatibility.1 aZhu, Zhiyuan1 aJeong, Gookyeong1 aKim, Seung-Jin1 aGadwal, Ikhlas1 aChoe, Youngson1 aBang, Joona1 aOh, Min-Kyu1 aKhan, Anzar1 aRao, Jingyi uhttps://library.ncl.res.in/content/balancing-antimicrobial-performance-hemocompatibility-amphiphilic-homopolymers01562nas a2200145 4500008004100000245017100041210007100212260000800283300001200291490000600303520090900309100003201218700002601250856014001276 2018 eng d00aBase‐promoted heterogeneous reusable copper fluorapatite (CuFAP) catalyzed facile synthesis of 1,2‐diarylethanols via C(sp3)‐H functionalization of nitrotoluene0 aBase‐promoted heterogeneous reusable copper fluorapatite CuFAP c cJAN a719-7230 v33 aA novel, an efficient protocol has been developed for the facile synthesis of various 1,2‐diarylethanol in good to excellent yields via C(sp3)‐H functionalization of various nitrotoluenes with substituted aromatic, heterocyclic aldehydes/isatin. This is the first example in which base promoted heterogeneous, reusable copper fluorapatite (CuFAP) catalyzed C(sp3)‐H functionalization of various nitrotoluenes was achieved under ligand‐, additive‐ free reaction condition. The developed protocol is an environmentally benign approach, which replaces cumbersome protocols, avoids the use of costly precious metal catalysts, prefunctionalized substrates, and toxic reagents. The heterogeneous CuFAP catalyst was recovered by simple filtration and reused several times without the loss of its catalytic activity. The selective functional group transformation of 1,2‐diaryethanol was accomplished.1 aShaikh, Taufeekaslam, M. Y.1 aMulla, Shafeek, A. R. uhttps://library.ncl.res.in/content/base%E2%80%90promoted-heterogeneous-reusable-copper-fluorapatite-cufap-catalyzed-facile-synthesis-1201982nas a2200241 4500008004100000022001400041245010800055210006900163260000800232300001400240490000600254520113500260653001801395653003001413653001301443653002501456653002301481653003301504653003401537100002501571700001701596856012701613 2018 eng d a2155-543500aB(C6F5)(3): catalyst or initiator? insights from computational studies into surrogate silicon chemistry0 aBC6F53 catalyst or initiator insights from computational studies cJUL a6163-61760 v83 a

One of the most promising recent developments in catalysis has been the use of the metal-free Lewis acid B(C6F5)(3) as a catalyst for a range of different chemical transformations. Perhaps the most impressive achievement in this regard is the recently accomplished in situ generation of SiH4 from surrogates (Simonneau and Oestreich, Nat. Chem., 2015,7, 816). However, what the current computational work, with density functional theory, reveals is that this process, in addition to being catalyzed by B(C6F5)(3), is also significantly dominated by a series of autocatalytic reactions. The results are further corroborated by the use of the energetic span model, which shows that the turnover frequency is higher for the newly proposed autocatalytic pathway in comparison to the conventional B(C6F5)(3)-catalyzed pathway. The current work therefore provides interesting new insights into surrogate silicon chemistry. But, more importantly, the current studies indicate that B(C6F5)(3) is likely to function more as an initiator rather than a pure catalyst in many metal-free transformations that have been reported to date.

10aautocatalysis10aDensity functional theory10aion-pair10aLewis acid catalysis10areaction mechanism10asurrogate silicone chemistry10atris(pentafluorophenyl)borane1 aBanerjee, Subhrashis1 aVanka, Kumar uhttps://library.ncl.res.in/content/bc6f53-catalyst-or-initiator-insights-computational-studies-surrogate-silicon-chemistry01312nas a2200145 4500008004100000245007400041210006900115260000800184300001400192490000600206520082200212100001701034700001601051856009901067 2018 eng d00aBenzoin condensation: a kinetic monitoring at the oil-water interface0 aBenzoin condensation a kinetic monitoring at the oilwater interf cMAY a4694-46980 v33 aThe overall kinetics of condensation of benzaldehyde to benzoin has been discussed here with an aim to develop a better understanding of the change in reactivity of the interfacial reaction in the presence of co-solvents. A key aspect of the 'on water' reaction is the chemistry between solvent and reactants that occur at the oil-water interface. In particular the orientation of water molecules at the interface plays a key role in catalyzing the reaction. An initial increase in the rate constants of reaction is observed, followed by sharp fall in the reaction kinetics. An interesting interplay of hydrophobicity, hydrogen bonding and polarity of the binary mixture is inevitable in the present study. The observations are nevertheless important reference point for future investigations of 'on water' reactions.1 aSingh, Anshu1 aKumar, Anil uhttps://library.ncl.res.in/content/benzoin-condensation-kinetic-monitoring-oil-water-interface01448nas a2200157 4500008004100000245011600041210006900157260000800226300001000234490000800244520083200252100002601084700002601110700002401136856013001160 2018 eng d00aBessel's polynomial fitting for electrospun polyacrylonitrile/polyaniline blend nanofibers based ammonia sensor0 aBessels polynomial fitting for electrospun polyacrylonitrilepoly cJUL a70-730 v2213 aIn the present paper, we report the fabrication of electrospun polyacrylonitrile/polyaniline (PAN/PANI) blend nanofibers by electrospinning and polymerization and Bessel's polynomial model applied for its ammonia sensing characteristics. As-fabricated PAN/PANI blend nanofibers were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy for the confirmation of fibers with nanoscale and blends of PAN and PANI. The semiconducting behavior of the PAN/PANI blend nanofibers was found to respond quickly towards ammonia gas. Sensitivity of the blend was obtained at near room temperature for different concentrations of ammonia. Bessel's polynomial function was found to be well fitted with the experimental data for the response towards ammonia gas. (C) 2018 Elsevier B.V. All rights reserved.1 aAnwane, Rajashree, S.1 aKondawar, Subhash, B.1 aLate, Dattatray, J. uhttps://library.ncl.res.in/content/bessels-polynomial-fitting-electrospun-polyacrylonitrilepolyaniline-blend-nanofibers-based01733nas a2200241 4500008004100000022001400041245012100055210006900176260000800245300001400253490000700267520090400274653001701178653001401195653002001209653003601229653002601265100001901291700001701310700001701327700001901344856012801363 2018 eng d a0947-653900aBeyond hydrofunctionalisation: a well-defined calcium compound catalysed mild and efficient carbonyl cyanosilylation0 aBeyond hydrofunctionalisation a welldefined calcium compound cat cJAN a1269-12730 v243 a

Organocalcium compounds have been reported as efficient catalysts for various transformations, for cases in which one of the substrates contained an E-H (E=B, N, Si, P) bond. Here, we look at the possibility of employing an organocalcium compound for a transformation in which none of the precursors has a polar E-H bond. This study demonstrates the utilization of a well-defined amidinatocalcium iodide, [PhC(NiPr)(2)CaI] (1) for cyanosilylation of a variety of aldehydes and ketones with Me3SiCN under ambient conditions without the need of any co-catalyst. The reaction mechanism involves a weak adduct formation between 1 and Me3SiCN leading to the activation of the Si-C bond, which subsequently undergoes sigma-bond metathesis with a C=O moiety. Such a mechanistic pathway is unprecedented in alkaline earth metal chemistry. Experimental and computational studies support the mechanism.

10aCa catalysis10acarbonyls10acyanosilylation10aDensity functional calculations10atransition-metal free1 aYadav, Sandeep1 aDixit, Ruchi1 aVanka, Kumar1 aSen, Sakya, S. uhttps://library.ncl.res.in/content/beyond-hydrofunctionalisation-well-defined-calcium-compound-catalysed-mild-and-efficient01879nas a2200169 4500008004100000245010200041210006900143260000800212300001200220490000800232520125300240100002101493700002401514700001901538700002901557856012301586 2018 eng d00aBifunctional role of Pd/MMT-K 10 catalyst in direct transformation of furfural to 1,2-pentanediol0 aBifunctional role of PdMMTK 10 catalyst in direct transformation cJUL a195-2010 v3093 a

The acid functionality of montmorillonite clay K 10 (MMT-K 10) was found to play a key role to give 1,2-pentanediol (1,2-PeDO) in Pd catalysed furfural hydrogenation. Among various Pd loadings on MMT-K 10, 3% Pd/MMT-K 10 catalyst exhibited excellent performance giving almost complete conversion of FFR and the highest selectivity of 66% to 1,2-PeDO. Py-IR of the catalyst evidenced the presence of Brønsted acidity which was responsible in C5-O cleavage of furan ring after the formation of furfuryl alcohol which is the first step intermediate in FFR hydrogenation. At a lower temperature of 140 °C, highest selectivity of 56% was achieved for FAL while increase in temperature to 220 °C, enhanced the selectivity to 1,2-PeDO. Keeping the temperature constant at 220 °C, with increase in H2 pressure from 500 to 750 psig, resulted in decrease in 1,2-PeDO selectivity from 66 to 34% with proportionate increase in THFAL selectivity. Thus as per the requirement, the product selectivities can be tailored by varying the reaction parameters suitably. Several control experiments were also performed the results of which combined with the characterization data allowed to propose a plausible reaction pathway for the formation of 1,2-PeDO.

1 aDate, Nandan, S.1 aChikate, Rajeev, C.1 aRoh, Hyun-Seog1 aRode, Chandrashekhar, V. uhttps://library.ncl.res.in/content/bifunctional-role-pdmmt-k-10-catalyst-direct-transformation-furfural-12-pentanediol01922nas a2200181 4500008004100000245012700041210006900168260000800237300001000245490000800255520125900263100001701522700002001539700002101559700001601580700001701596856012701613 2018 eng d00aBimetallic Au-Ag/Al2O3 as efficient catalysts for the hydrocarbon selective reduction of NOx from lean burn engine exhaust0 aBimetallic AuAgAl2O3 as efficient catalysts for the hydrocarbon  cMAY a23-310 v3063 aCatalytic properties of Au-Ag/Al2O3 catalysts, prepared by successive impregnation, have been investigated for the hydrocarbon selective catalytic reduction (HC-SCR) of NOx. The performances of pre-reduced and aged catalysts were evaluated on synthetic reaction mixtures with gas compositions representative of the exhaust of Diesel powered engines. An optimal 2 wt.% Ag loading was chosen and the order of introduction during sequential impregnation of Ag or Au on respectively pre-impregnated Au/Al2O3 and Ag/Al2O3 substrates was investigated revealing drastic changes on the catalytic properties after aging. XPS analysis shows a significant silver enrichment irrespective of the preparation route and aging conditions whereas ex situ UV-vis DRS analysis reveals significant electronic disturbances which reflect different interactions between Au and Ag in fresh and aged Au-Ag/Al2O3 and Ag-Au/Al2O3 catalysts. As a consequence, significant rate enhancements in NOx conversion were observed with the superior performance of Au-Ag/Al2O3 but a lower resistance to deactivation at high temperature explained by different behaviour towards redispersion/agglomeration processes highlighted from TEM observations. (C) 2016 Elsevier B.V. All rights reserved.1 aMore, P., M.1 aDongare, M., K.1 aUmbarkar, S., B.1 aGranger, P.1 aDujardin, C. uhttps://library.ncl.res.in/content/bimetallic-au-agal2o3-efficient-catalysts-hydrocarbon-selective-reduction-nox-lean-burn01830nas a2200193 4500008004100000245010900041210006900150260000800219300001600227490000600243520112700249100002201376700002401398700002301422700002501445700001901470700002001489856012701509 2018 eng d00aBiomass-mediated synthesis of Cu-doped TiO2 nanoparticles for improved-performance lithium-ion batteries0 aBiomassmediated synthesis of Cudoped TiO2 nanoparticles for impr cOCT a13676-136840 v33 a

Pure TiO2 and Cu-doped TiO2 nanoparticles are synthesized by the biomediated green approach using the Bengal gram bean extract. The extract containing biomolecules acts as capping agent, which helps to control the size of nanoparticles and inhibit the agglomeration of particles. Copper is doped in TiO2 to enhance the electronic conductivity of TiO2 and its electrochemical performance. The Cu-doped TiO2 nanoparticle-based anode shows high specific capacitance, good cycling stability, and rate capability performance for its envisaged application in lithium-ion battery. Among pure TiO2, 3% Cu-doped TiO2, and 7% Cu-doped TiO2 anode, the latter shows the highest capacity of 250 mAh g(-1) (97.6% capacity retention) after 100 cycles and more than 99% of coulombic efficiency at 0.5 A g(-1) current density. The improved electrochemical performance in the 7% Cu-doped TiO2 is attributed to the synergetic effect between copper and titania. The results reveal that Cu-doped TiO2 nanoparticles might be contributing to the enhanced electronic conductivity, providing an efficient pathway for fast electron transfer.

1 aKashale, Anil, A.1 aDwivedi, Pravin, K.1 aSathe, Bhaskar, R.1 aShelke, Manjusha, V.1 aChang, Jia-Yaw1 aGhule, Anil, V. uhttps://library.ncl.res.in/content/biomass-mediated-synthesis-cu-doped-tio2-nanoparticles-improved-performance-lithium-ion02028nas a2200205 4500008004100000022001400041245011800055210006900173260000900242490000700251520132500258100001801583700001401601700001601615700001701631700001401648700001401662700001901676856012701695 2018 eng d a1613-451600aBIOPYDB: a dynamic human cell specific biochemical pathway database with advanced computational analyses platform0 aBIOPYDB a dynamic human cell specific biochemical pathway databa cMAR 0 v153 aBIOPYDB: BIOchemical PathwaY DataBase is developed as a manually curated, readily updatable, dynamic resource of human cell specific pathway information along with integrated computational platform to perform various pathway analyses. Presently, it comprises of 46 pathways, 3189 molecules, 5742 reactions and 6897 different types of diseases linked with pathway proteins, which are referred by 520 literatures and 17 other pathway databases. With its repertoire of biochemical pathway data, and computational tools for performing Topological, Logical and Dynamic analyses, BIOPYDB offers both the experimental and computational biologists to acquire a comprehensive understanding of signaling cascades in the cells. Automated pathway image reconstruction, cross referencing of pathway molecules and interactions with other databases and literature sources, complex search operations to extract information from other similar resources, integrated platform for pathway data sharing and computation, etc. are the novel and useful features included in this database to make it more acceptable and attractive to the users of pathway research communities. The RESTful API service is also made available to the advanced users and developers for accessing this database more conveniently through their own computer programmes.1 aChowdhury, S.1 aSinha, N.1 aGanguli, P.1 aBhowmick, R.1 aSingh, V.1 aNandi, S.1 aSarkar, R., R. uhttps://library.ncl.res.in/content/biopydb-dynamic-human-cell-specific-biochemical-pathway-database-advanced-computational01559nas a2200157 4500008004100000245015100041210006900192260000800261300001400269490000700283520091300290100002401203700002801227700002201255856012401277 2018 eng d00aBismuth(III)-catalyzed cycloisomerization and (hetero)arylation of alkynols: simple access to 2-(hetero)aryl tetrahydrofurans and tetrahydropyrans0 aBismuthIIIcatalyzed cycloisomerization and heteroarylation of al cMAY a3229-32400 v163 a2-(Hetero)aryl tetrahydrofurans and tetrahydropyrans were successfully synthesized using Bi(OTf)(3)-catalyzed hydroalkoxylation (cycloisomerization) of alkynols (via 5 or 6 exo-dig cyclization) and intermolecular (hetero)arylation. This reaction involves a highly efficient cascade process, where initially the alkynol undergoes a cycloisomerization step via activation of the triple bond and generates the oxocarbenium ion, which subsequently participates in the (hetero)hydroarylation step with electron-rich arenes. Simple to complex suitably functionalized alkynols (4-pentyn-1-ols and 5-hexyn-1-ols) and electron-rich aromatic compounds were found to be reliable substrates in this cascade transformation and furnished a wide range of oxygen heterocycles. This practical tandem process provides a means to build libraries related to pharmacologically active molecules and natural product like scaffolds.1 aNakate, Ashwini, K.1 aPratapure, Madhukar, S.1 aKontham, Ravindar uhttps://library.ncl.res.in/content/bismuthiii-catalyzed-cycloisomerization-and-heteroarylation-alkynols-simple-access-201714nas a2200193 4500008004100000022001400041245008300055210006900138260000900207300001400216490000800230520107100238100002201309700001801331700001901349700001801368700002101386856011301407 2018 eng d a0011-389100a Bubble size prediction in gas-solid fluidized beds using genetic programming 0 aBubble size prediction in gassolid fluidized beds using genetic  cNOV  a1904-19120 v1153 aThe hydrodynamics of a gas-solid fluidized bed (FB) is affected by the bubble diameter, which in turn strongly influences the performance of a fluidized bed reactor (FBR). Thus, determining the bubble diameter accurately is of crucial importance in the design and operation of an FBR. Various equations are available for calculating the bubble diameter in an FBR. It has been found in this study that these models show a large variation while predicting the experimentally measured bubble diameters. Accordingly, the present study proposes a new equation for computing the bubble diameter in a fluidized bed. This equation has been developed using an efficient, yet infrequently employed computational intelligence (CI)-based data-driven modelling method termed genetic programming (GP). The prediction and generalization performance of the GP-based equation has been compared with that of a number of currently available equations for computing the bubble diameter in a fluidized bed and the results obtained show a good performance by the newly developed equation.1 aSonolikar, R., R.1 aPatil, M., P.1 aMankar, R., B.1 aTambe, S., S.1 aKulkarni, B., D. uhttps://library.ncl.res.in/content/bubble-size-prediction-gas-solid-fluidized-beds-using-genetic-programming01801nas a2200265 4500008004100000022001400041245007700055210006900132260000800201300001100209490000800220520092900228653002201157653001401179653002501193653002901218653002401247653001601271100002901287700002801316700002001344700002601364700003601390856010901426 2019 eng d a0003-986100aBaicalein suppresses repeat tau fibrillization by sequestering oligomers0 aBaicalein suppresses repeat tau fibrillization by sequestering o cOCT a1081190 v6753 a

Alzheimer's disease (AD) is a neurodegenerative disorder caused by protein misfolding, aggregation and accumulation in the brain. A large number of molecules are being screened against these pathogenic proteins but the focus for therapeutics is shifting towards the natural compounds as aggregation inhibitors, mainly due to their minimum adverse effects. Baicalein is a natural compound belonging to the class of flavonoids isolated from the Chinese herb Scutellaria baicalensis. Here we applied fluorescence, absorbance, microscopy, MALDI-TOF spectrophotometry and other biochemical techniques to investigate the interaction between Tau and Baicalein in vitro. We found the aggregation inhibitory properties of Baicalein for the repeat Tau. Overall, the potential of Baicalein in dissolving the preformed Tau oligomers as well as mature fibrils can be of utmost importance in therapeutics for Alzheimer's disease.

10aAlzheimer disease10aBaicalein10aMicrotubule assembly10aPaired helical filaments10aProtein aggregation10atau protein1 aSonawane, Shweta, Kishor1 aBalmik, Abhishek, Ankur1 aBoral, Debjyoti1 aRamasamy, Sureshkumar1 aChinnathambi, Subashchandrabose uhttps://library.ncl.res.in/content/baicalein-suppresses-repeat-tau-fibrillization-sequestering-oligomers02152nas a2200253 4500008004100000022001400041245013200055210006900187260000800256300001200264490000700276520130600283653000901589653001301598653002701611653002701638653001501665653001901680100001801699700002201717700002001739700002001759856011901779 2019 eng d a1214-021X00aBeneficial effects of Stevioside on AGEs, blood glucose, lipid profile and renal status in streptozotocin-induced diabetic rats0 aBeneficial effects of Stevioside on AGEs blood glucose lipid pro cAPR a190-1970 v173 a

The advanced glycated end products (AGEs) are formed in the diabetic patients; it is a major cause of macrovascular and microvascular complications in diabetes. Clinically there is no treatment available for the AGEs. Stveoside (Stv), a sweetener has potent anti-diabetic and anti-oxidant activity. Hence, we investigated its use in prevention of AGEs formation using in vitro and in vivo models. Diabetes was induced by streptozotocin (STZ). These rats were kept without treatment till blood HbA1c was markedly increased. They were then divided into 5 groups and treated orally with vehicle or Metformin (MET) or Stv respectively for 28 days. Every 7th day, animals were tested for body weight and blood glucose (BG). On the last day of treatment, all the groups were evaluated for physiological and biochemical parameters, histopathology and AGEs; N-carboxymethyl-lysine (CML) estimation. Stv showed inhibition of AGEs in in vitro as well as in in vivo respectively. Positive effects were seen on the BG, lipid profile and urine parameters as well it showed reduced formation of CML. It also showed antihyperglycaemic, antihyperlipedemic and nephroprotective activities. The present study provides scientific rationale for the use of Stv as a sweetener with additional benefits in diabetes.

10aAGEs10aDiabetes10aDiabetes complications10aN-carboxymethyl-lysine10aStevioside10aStreptozotocin1 aAswar, Urmila1 aGogawale, Vinayak1 aMiniyar, Pankaj1 aPatil, Yugendra uhttps://library.ncl.res.in/content/beneficial-effects-stevioside-ages-blood-glucose-lipid-profile-and-renal-status01712nas a2200289 4500008004100000022001400041245018100055210006900236260000900305300001400314490000600328520076400334653000601098653000601104653001601110653002201126653002001148653001301168653002001181653002701201100000501228700002701233700000501260700000501265700002801270856012401298 2019 eng d a2365-654900aBentonite - Clay - supported cuprous iodide nanoparticles (BENT- CuI NPs): a new heterogeneous catalyst in diversity - oriented synthesis of 1, 2, 3-triazoles in aqueous medium0 aBentonite Clay supported cuprous iodide nanoparticles BENT CuI N cJUN  a7144-71500 v43 a

Bentonite clay supported cuprous iodide nanoparticles (BENT-CuI NPs) were prepared and demonstrated for the first time as an efficient heterogeneous catalyst in diversity oriented, green synthesis of 1,2,3-triazoles by one-pot, three component reaction between alkyl halides, arylboronic acids, or epoxides with alkynes and sodium azide in aqueous medium. The catalyst was characterized by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES), Energy Dispersive X-ray Spectroscopy(EDS), Scanning Electron Microscopy (SEM), X-Ray Powder Diffraction (XRD), Transmission Electron Microscopy (TEM) as well as Brunauer-Emmett-Teller (BET) techniques and was found to be reusable for five consecutive runs without significant loss of activity.

10a110a210a3-triazoles10aarylboronic acids10aclick synthesis10aepoxides10agreen chemistry10aheterogeneous catalyst1 a1 aCharate, Shrinivas, P.1 a1 a1 aWadgaonkar, Prakash, P. uhttps://library.ncl.res.in/content/bentonite-clay-supported-cuprous-iodide-nanoparticles-bent-cui-nps-new-heterogeneous00425nas a2200145 4500008004100000022001400041245004400055210004100099260000800140300001400148490000800162100001600170700001600186856007700202 2019 eng d a0011-389100aBhaskar Dattatraya Kulkarni (1949-2019)0 aBhaskar Dattatraya Kulkarni 19492019 cAPR a1261-12620 v1161 aKumar, Ravi1 aSivaram, S. uhttps://library.ncl.res.in/content/bhaskar-dattatraya-kulkarni-1949-201902542nas a2200157 4500008004100000245009300041210006900134260000800203300001200211490000600223520198100229100001802210700001702228700001702245856012202262 2019 eng d00aBifunctional oxygen reduction and evolution activity in brownmillerites Ca2Fe(1- x)CoxO50 aBifunctional oxygen reduction and evolution activity in brownmil cJAN a31–380 v43 aState-of-the-art catalysts for oxygen reduction and evolution reactions (ORR and OER), which form the basis of advanced fuel cell applications, are based on noble metals such as Pt and Ir. However, high cost and scarcity of noble metals have led to an increased demand of earth-abundant metal oxide catalysts, especially for bifunctional activity in ORR and OER. The fact that Pt and Ir or C, the cost-effective alternatives suggested, do not display satisfactory bifunctional activity has also helped in turning the interest to metal oxides which are stable under both ORR and OER conditions. Brownmillerite A2B2O5 type oxides are promising as bifunctional oxygen electrocatalysts because of intrinsic structural features, viz., oxygen vacancy and catalytic activity of the B-site transition metal. In this study, Co-doped Ca2Fe2O5 compounds are synthesized by the solid state method and structurally analyzed by Rietveld refinement of powder X-ray diffraction data. The compound Ca2Fe2O5, crystallizing in the Pcmn space group has alternative FeO4 tetrahedral and FeO6 octahedral layers. Its Co-doped analogue, Ca2Fe1.75Co0.25O5, also crystallizes in the same space group with both tetrahedral and octahedral Fe positions substituted with Co. However, Ca2FeCoO5 in the Pbcm space group shows interlayer ordering with Co-rich octahedra connected to Fe-rich tetrahedra and vice versa. Oxygen bifunctional activities of these catalysts are monitored by rotating disc electrode and rotating ring disc electrode techniques in alkaline media. A close analysis of the ORR and OER was conducted through comparison of various parameters such as onset potential, current density, halfwave potential, and other kinetic parameters, which suggests that the presence of Co in the B site aids in achieving better bifunctional activity and bulk conductivity. In addition, Co(II)/Co(III) redox systems and their comparative concentrations also play a decisive role in enhancing the activity.1 aThundiyil, S.1 aKurungot, S.1 aDevi, R., N. uhttps://library.ncl.res.in/content/bifunctional-oxygen-reduction-and-evolution-activity-brownmillerites-ca2fe1-xcoxo505035nas a2200205 4500008004100000245016600041210006900207260000800276300001400284490000600298520424600304100002504550700001904575700002404594700001404618700002104632700002304653700002204676856013104698 2019 eng d00aBioactivities and molecular networking-based elucidation of metabolites of potent actinobacterial strains isolated from the Unkeshwar geothermal springs in India0 aBioactivities and molecular networkingbased elucidation of metab cMAR a9850-98590 v93 a

The bioactive potential of Actinobacteria endemic to hot springs has rarely been investigated. This study highlights the cultivable diversity and bioactivities of Actinobacteria associated with the Unkeshwar hot springs, India. Potent strains were evaluated for their biosynthetic potentials and metabolite analysis was performed using effective dereplication molecular networking tools. A total of 86 actinobacterial strains were isolated and grouped into 21 distinct genera, based on 16S rRNA gene sequence analysis. These strains included rare members such as Micromonospora, Marmoricola, Actinomadura, Cellulomonas, Cellulosimicrobium, Janibacter, Rothia, Barrentisimonas, Dietzia and Glycomyces. In antimicrobial screening, Micromonospora sp. strain GH99 and Streptomyces sp. strain GH176 were found to be potent antimicrobial strains. The metabolic extracts of these strains exhibited strong antimicrobial activity against Staphylococcus epidermidis (NCIM 2493), Shigella flexneri (NCIM 5265), Klebsiella pneumonia (NCIM 2098), and Salmonella abony (NCIM 2257). The extracts also displayed strong anti-biofilm and anticancer activities against Pseudomonas aeruginosa (NCIM 5029), Acinetobacter junii (NCIM 5188) and breast cancer cell line MCF7, respectively. Both strains also tested positive for the presence of the PKS biosynthetic gene cluster in their genomes. To effectively delineate the secondary metabolites, the extracts were subjected to MS/MS-guided molecular networking analysis. Structurally diverse compounds including the polyketides 22-dehydroxymethyl-kijanolide (GH99 strain) and Abyssomicin I (GH176 strain) were detected in the extracts. Interestingly, Brevianamide F was detected in the extract of Micromonospora, which has previously been mostly found in fungal species. Other compounds such as cyclic tripeptides, Cyclo(l-Pro-d-Ile) and Cyclo(d-Pro-l-Phe), were also identified in this strain. In summary, for the first time, we explored the diversity of Actinobacteria and evaluated their bioactive potential from the Unkeshwar hot springs. The potent strains isolated in the study could be useful in drug discovery programs.

1 aMehetre, Gajanan, T.1 aVinodh, J., S.1 aBurkul, Bhushan, B.1 aDesai, D.1 aSanthakumari, B.1 aDharne, Mahesh, S.1 aDastager, Syed, G uhttps://library.ncl.res.in/content/bioactivities-and-molecular-networking-based-elucidation-metabolites-potent-actinobacterial01639nas a2200157 4500008004100000245006100041210006100102260001300163300001000176520111400186100002101300700002201321700002201343700002601365856009001391 2019 eng d00aBiochemical and molecular aspects of dimorphism in fungi0 aBiochemical and molecular aspects of dimorphism in fungi bSpringer a69-943 aMost of the eukaryotic differentiation processes are unidirectional. However, fungi have the ability to grow reversibly as unicellular yeast (Y) or as filamentous hypha (H) in response to the specific strain-dependent environmental stimuli. Such a phenomenon known as “dimorphism” is not limited to a specific class of fungi. Most of the plant, human, and insect pathogenic fungi show Y-H and reversible morphogenesis, associated with their saprophytic to pathogenic change, for survival and proliferation in the host. In this chapter, we have described the factors stimulating dimorphism, the signal transduction pathways induced by these stimuli, changes in the gene/protein expression patterns due to a cascade of these signals, and, finally, translation of this genotypic effect into phenotypic change, i.e., the morphological outcome. The process of fungal differentiation and formation of tumor cells follow the same regulatory series of events, involving cAMP, MAP, and RAS kinase cascades. Therefore, the molecules inhibiting Y-H transition in fungi can be explored for their anticancer potential.1 aPathan, Ejaj, K.1 aGhormade, Vandana1 aPanmei, Redeemson1 aDeshpande, Mukund, V. uhttps://library.ncl.res.in/content/biochemical-and-molecular-aspects-dimorphism-fungi02891nas a2200241 4500008004100000022001400041245015700055210006900212260000800281300001000289490000800299520204400307653001902351653001902370653001402389653001802403653000902421653001702430100002502447700002302472700002302495856013102518 2019 eng d a0048-969700aBiodegradation of mixed polycyclic aromatic hydrocarbons by pure and mixed cultures of biosurfactant producing thermophilic and thermo-tolerant bacteria0 aBiodegradation of mixed polycyclic aromatic hydrocarbons by pure cAUG a52-600 v6793 a

Applicability of thermophilic and thereto-tolerant microorganisms for biodegradation of polycyclic aromatic hydrocarbons (PAHs) with low water solubility is an interesting strategy for improving the biodegradation efficiency. In this study, we evaluated utility of thermophilic and thermo-tolerant bacteria isolated from Unkeshwar hot spring (India) for biodegradation of four different PAHs. Water samples were enriched in mineral salt medium (MSM) containing a mixture of four PAHs compounds (anthracene: ANT, fluorene: FLU, phenanthrenc: PHE and pyrene: PYR) at 37 degrees C and 50 degrees C. After growth based screening, four potent strains obtained which were identified as Aeribacillus pallidus (UCPS2), Bacillus axarquiensis (UCPD1), Bacillus siamensis (GHP76) and Bacillus subtilis subsp. inaquosorum (U277) based on the 16S rRNA gene sequence analysis. Degradation of mixed PAH compounds was evaluated by pure as well as mixed cultures under shake flask conditions using MSM supplemented with 200 mg/L concentration of PAHs (50 mg/L of each compound) for 15 days at 37 degrees C and 50 degrees C. A relatively higher degradation of ANT (92% - 96%), FLU (83% - 86%), PHE (16% - 54%) and PYR (51% - 71%) was achieved at 50 degrees C by Aeribacillus sp. (UCPS2) and mixed culture. Furthermore, crude oil was used as a substrate to study the degradation of same PAHs using these organisms which also revealed with similar results with the higher degradation at 50 degrees C. Interestingly, PAH-degrading strains were also positive for biosurfactant production. Biosurfactants were identified as the variants of surfactins (lipopeptide biosurfactants) based on analytical tools and phylogenetic analysis of the surfactin genes. Overall, this study has shown that hot spring microbes may have a potential for PAHs degradation and also biosurfactant production at a higher temperature, which could provide a novel perspective for removal of PAHs residues from oil contaminated sites. (C) 2019 Elsevier B.V. All rights reserved.

10aBiodegradation10aBiosurfactants10aCrude oil10aMixed culture10aPAHs10aThermophiles1 aMehetre, Gajanan, T.1 aDastager, Syed, G.1 aDharne, Mahesh, S. uhttps://library.ncl.res.in/content/biodegradation-mixed-polycyclic-aromatic-hydrocarbons-pure-and-mixed-cultures-biosurfactant02638nas a2200253 4500008004100000022001400041245013500055210006900190260000800259300001200267490000800279520176200287653002702049653001602076653002302092653001502115653002802130100002102158700002202179700001702201700002002218700002002238856012602258 2019 eng d a0964-830500aBiodegradation of squalene and n-hexadecane by Gordonia amicalis HS-11 with concomitant formation of biosurfactant and carotenoids0 aBiodegradation of squalene and nhexadecane by Gordonia amicalis  cAUG a172-1810 v1423 a

Gordonia amicalis HS-11 has been enriched from a hydrocarbon contaminated tropical soil sample. The ability of this organism to utilize a triterpenic polyunsaturated hydrocarbon, squalene (2,6,10,15,19,23-hexamethyl-6,6,10,14,18,20-tetracosahexane) and the model saturated hydrocarbon n-hexadecane is described here. The isolate degraded squalene and n-hexadecane (79 +/- 3.02 and 96 +/- 4.11%, respectively) after eight days of incubation. The isolate produced an extracellular biosurfactant that reduced surface tension from 69 +/- 2.83 to 40 +/- 1.63 and 35 +/- 2.34 mN m(-1) with squalene and n-hexadecane as carbon sources, respectively, after 6 days. The Actinomycete cleaved squalene to geranylacetone and famesyl acetaldehyde that were further utilized for supporting growth. n-Hexadecane was degraded via monoterminal oxidation and activities of important enzymes (alkane hydroxylase and alcohol dehydrogenase) were highest (215 +/- 8.76 and 169 +/- 6.02 units mg(-1) protein, respectively) after four days. Cells grown on squalene were short and with n-hexadecane there were clumps of longer cells. Squalene and n-hexadecane-grown cell surfaces were smooth possibly due to extracellular surface active compounds. While growing on hydrophobic substrates, some cells were seen adhering to droplets and others were in the free form. The culture was able to simultaneously degrade hydrocarbons and produce two commercially relevant value-added products. The yield of the extracellular biosurfactant on n-hexadecane was 480 mg l(-1) and cells grown on squalene and n-hexadecane also yielded carotenoids (2.3 +/- 0.14 and 2.9 +/- 0.10 mg g(-1) dry cell weight, respectively). This is the first report on the utilization of squalene by Gordonia.

10aAerobic biodegradation10aCarotenoids10aCatabolic pathways10aEmulsifier10aGordonia amicalis HS-111 aSowani, Harshada1 aDeshpande, Ashish1 aGupta, Vidya1 aKulkarni, Mohan1 aZinjarde, Smita uhttps://library.ncl.res.in/content/biodegradation-squalene-and-n-hexadecane-gordonia-amicalis-hs-11-concomitant-formation02469nas a2200193 4500008004100000022001400041245010500055210006900160260000800229520178200237653001302019653001502032653002602047653001402073100002002087700002202107700002202129856012402151 2019 eng d a1861-472800aBiopolymer/glycopolypeptide-blended scaffolds: synthesis, characterization and cellular interactions0 aBiopolymerglycopolypeptideblended scaffolds synthesis characteri cDEC3 a

Three-dimensional (3D) scaffolds formed from natural biopolymers gelatin and chitosan that are chemically modified by galactose have shown improved hepatocyte adhesion, spheroid geometry and functions of the hepatocytes. Galactose specifically binds to the hepatocytes via the asialoglycoprotein receptor (ASGPR) and an increase in galactose density further improves the hepatocyte proliferation and functions. In this work, we aimed to increase the galactose density within the biopolymeric scaffold by physically blending the biopolymers chitosan and gelatin with an amphiphlic beta-galactose polypeptide (PPO-GP). PPO-GP, is a di-block copolymer with PPO and beta-galactose polypeptide, exhibits lower critical solution temperature and is entrapped within the scaffold through hydrophobic interactions. The uniform distribution of PPO-GP within the scaffold was confirmed by fluorescence microscopy. SEM and mechanical testing of the hybrid scaffolds indicated pore size, inter connectivity and compression modulus similar to the scaffolds made from 100 % biopolymer. The presence of the PPO-GP on the surface of the scaffold was tested monitoring the interaction of an analogous mannose containing PPO-GP scaffold and the mannose binding lectin Con-A. In vitro cell culture experiments with HepG2 cells were performed on GLN-GP and CTS-GP and their cellular response was compared with GLN and CTS scaffolds for a period of seven days. Within three days of culture the Hep G2 cells formed multicellular spheroids on GLN-GP and CTS-GP more efficiently than on the GLN and CTS scaffolds. The multicellular spheroids were also found to infiltrate more in GLN-GP and CTS-GP scaffolds and able to maintain their round morphology as observed by live/dead and SEM imaging.

10aadhesion10aAggregates10aCellular infiltration10aSpheroids1 aDhaware, Vinita1 aDiaz, David, Diaz1 aGupta, Sayam, Sen uhttps://library.ncl.res.in/content/biopolymerglycopolypeptide-blended-scaffolds-synthesis-characterization-and-cellular01532nas a2200145 4500008004100000245005600041210005400097260001300151300001200164520106400176100001901240700001801259700002201277856008701299 2019 eng d00aBioprospecting of fungal entomo- and myco-pathogens0 aBioprospecting of fungal entomo and mycopathogens bSpringer a497-5133 aThe pest and pathogen control in the field using fungi and their metabolites has indeed gone beyond ‘proof of concept’. However, in view of the performances of these biocontrol fungi in the fields, moreover, acceptability by the end users regarding cost-effectiveness, shelf life, intellectual property rights (IPR), the additional roles, and possible applications are being explored. Dual pathogenicity, viz. entomopathogenesis and mycoparasitism, has added the advantage of wide-spectrum biocontrol in single-crop system. In addition to plant protection, these fungi can promote plant growth. Further, in integrated pest management, the bioremediation of pesticide residues, if any, can also be achieved by these organisms. For value addition, use of cuticle-degrading and mycolytic enzymes produced as killing components can be explored in food, detergent, organic synthesis, and pharmaceutical industries. While exploring the additional applications, the safety to beneficial insects and fungi, biodiversity, and humans, in general, is also addressed.1 aPathan, E., K.1 aPatil, A., V.1 aDeshpande, M., V. uhttps://library.ncl.res.in/content/bioprospecting-fungal-entomo-and-myco-pathogens02200nas a2200241 4500008004100000245017100041210006900212260000800281300001000289490000800299520133400307100002001641700001801661700001901679700001901698700002001717700001901737700001901756700001701775700001801792700001801810856013001828 2019 eng d00aBiosynthesized Co-doped TiO2 nanoparticles based anode for lithium-ion battery application and investigating the influence of dopant concentrations on its performance0 aBiosynthesized Codoped TiO2 nanoparticles based anode for lithiu cJUN a44-500 v1673 aTiO2 is a good alternative anode material for lithium-ion battery application because of its incomparable high structural stability and safety during the charge/discharge cycles. However, the low intrinsic conductivity of TiO2 has been a limiting factor affecting its cycling and rate capability performance. Here in this work, we present Co-doped TiO2 nanoparticles based anode with good reversibility, cycling stability and rate capability performance for its envisaged application in lithium-ion battery. The Co-doped TiO2 nanoparticles with different Co concentrations (3%, 5%, and 7%) are synthesized using simple and economic biomediated green approach, wherein TiCl4 and Co precursors are allowed to react in Bengal gram bean extract containing biomolecules which act as natural capping agents to control the size of nanoparticles. Among the pure TiO2 and different Co-doped TiO2 samples, the 7% Co-doped TiO2 anode show the highest capacity of 167 mAh g−1 (88.3%) after 100 cycles at the 0.5C current density. The Co-doped TiO2 shows higher and stable coulombic efficiency up to 100 GCD cycles indicating good reversibility. Based on the results, it is expected that the Co-doped TiO2 nanoparticles might be contributing to the enhanced electronic conductivity providing an efficient pathway for fast electron transfer.1 aKashale, A., A.1 aRasal, A., S.1 aKamble, G., P.1 aIngole, V., H.1 aDwivedi, P., K.1 aRajoba, S., J.1 aJadhav, L., D.1 aLing, Y., C.1 aChang, J., Y.1 aGhule, A., V. uhttps://library.ncl.res.in/content/biosynthesized-co-doped-tio2-nanoparticles-based-anode-lithium-ion-battery-application-and01774nas a2200169 4500008004100000245011600041210006900157260000800226300002200234490000800256520113900264100002001403700002401423700001501447700002001462856012201482 2019 eng d00aBorassus powder-reinforced poly(lactic acid) composites with improved crystallization and mechanical properties0 aBorassus powderreinforced polylactic acid composites with improv cJAN aArticle No: 474400 v1363 aThis article reports on the development of biocomposites based on polylactic acid (PLA) and borassus powder. Borassus powder was treated with alkali to remove hemicelluloses and lignin. The treated borassus improved the homogeneous mixing with PLA and increased the crystallinity of PLA. Dispersibility of the borassus was studied by scanning electron microscopy (SEM) and X‐ray MicroCT. PLA/borassus composites were prepared by melt mixing of PLA with 5, 10, and 15 wt % treated/untreated borassus. Composites were examined for mechanical properties and crystallization. Composites showed enhanced tensile strength compared to neat PLA. The PLA/treated borassus powder composites displayed higher crystallinity than PLA. The isothermal cold crystallization study showed increase in the crystallization rate of PLA in the presence of treated borassus. The spherulitic growth was studied using polarized optical microscopy. The enhanced performance of the PLA‐borassus composites was observed in the presence of borassus. This study demonstrates that the PLA‐borassus composites show great promise for bioplastics applications.1 aMarathe, Y., N.1 aArun, Torris, A. T.1 aRamesh, C.1 aBadiger, M., V. uhttps://library.ncl.res.in/content/borassus-powder-reinforced-polylactic-acid-composites-improved-crystallization-and01605nas a2200205 4500008004100000022001400041245008900055210006900144260000800213520090400221653001001125653001801135653002401153653001101177653001201188100003301200700001501233700003001248856012101278 2019 eng d a2192-650600aBoron-conjugated pyrenes as fluorescence-based molecular probes and security markers0 aBoronconjugated pyrenes as fluorescencebased molecular probes an cJUL3 a

Boron-embedded aromatic hydrocarbons are a class of molecules known for their distinct electronic and/or optoelectronic properties and are thus suitable for many potential applications. Among those, boronic ester and acid containing molecules have been widely used for sensing and molecular recognition applications, respectively. We compared the sensing and molecular recognition properties of two boron-containing pyrene derivatives for fluoride and glucose sensing applications. The presence of four boronate ester groups enabled fluoride ion sensing at the mu M level. The boronic acid derivative is very selective towards glucose compared to other saccharides. Furthermore, we used the mechano-responsive fluorescence changes and self-assembly of these derivatives, respectively, for fluorescence-based inkless and ink (water)-based writing in invisible security labeling applications.

10aboron10acarbohydrates10amechanoluminescence10apyrene10asensors1 aWakchaure, Vivek, Chandrakan1 aDas, Tamal1 aBabu, Sukumaran, Santhosh uhttps://library.ncl.res.in/content/boron-conjugated-pyrenes-fluorescence-based-molecular-probes-and-security-markers02223nas a2200289 4500008004100000245012600041210006900167260000800236300000900244490000700253520117200260653001301432653001301445653002001458653002101478653002601499653002601525100003801551700004201589700002301631700002801654700002701682700002701709700003701736700003401773856012601807 2020 eng d00aBacterial biofilm formation using PCL/curcumin electrospun fibers and its potential use for biotechnological applications0 aBacterial biofilm formation using PCLcurcumin electrospun fibers cDEC a55560 v133 a

Electrospun nanofibers are used for many applications due to their large surface area, mechanical properties, and bioactivity. Bacterial biofilms are the cause of numerous problems in biomedical devices and in the food industry. On the other hand, these bacterial biofilms can produce interesting metabolites. Hence, the objective of this study is to evaluate the efficiency of poly (x190;- caprolactone)/Curcumin (PCL/CUR) nanofibers to promote bacterial biofilm formation. These scaffolds were characterized by scanning electron microscopy (SEM), which showed homogeneous fibers with diameters between 441-557 nm; thermogravimetric analysis and differential scanning calorimetry (TGA and DSC) demonstrated high temperature resilience with degradation temperatures over >350 degrees C; FTIR and H-1-NMR serve as evidence of CUR incorporation in the PCL fibers. PCL/CUR scaffolds successfully promoted the formation of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa biofilms. These results will be valuable in the study of controlled harvesting of pathogenic biofilms as well as in metabolites production for biotechnological purposes.

10aBacteria10acurcumin10aelectrospinning10aEscherichia coli10aPseudomona aeruginosa10aStaphylococcus aureus1 aPompa-Monroy, Daniella, Alejandra1 aFigueroa-Marchant, Paulina, Guadalupe1 aDastager, Syed, G.1 aThorat, Meghana, Namdeo1 aIglesias, Ana, Leticia1 aMiranda-Soto, Valentin1 aPerez-Gonzalez, Graciela, Lizeth1 aVillarreal-Gomez, Luis, Jesus uhttps://library.ncl.res.in/content/bacterial-biofilm-formation-using-pclcurcumin-electrospun-fibers-and-its-potential-use02516nas a2200217 4500008004100000022001400041245008600055210006900141260000800210300000900218490000700227520175600234100002801990700001602018700003402034700001802068700002202086700003302108700003602141856012102177 2020 eng d a2045-232200aBasic limonoid modulates chaperone-mediated proteostasis and dissolve Tau fibrils0 aBasic limonoid modulates chaperonemediated proteostasis and diss cMAR a40230 v103 a

The Alzheimer's disease pathology is associated with accumulation of intracellular neurofibrillary tangles and extracellular senile plaques. The formation of initial nucleus triggers conformational changes in Tau and leads to its deposition. Hence, there is a need to eliminate these toxic proteins for proper functioning of neuronal cells. In this aspect, we screened the effect of basic limonoids such as gedunin, epoxyazadiradione, azadirone and azadiradione on inhibiting Tau aggregation as well as disintegration of induced Tau aggregates. It was observed that these basic limonoids effectively prevented aggregates formation by Tau and also exhibited the property of destabilizing matured Tau aggregates. The molecular docking analysis suggests that the basic limonoids interact with hexapeptide regions of aggregated Tau. Although these limonoids caused the conformational changes in Tau to beta-sheet structure, the cytological studies indicate that basic limonoids rescued cell death. The dual role of limonoids in Tau aggregation inhibition and disintegration of matured aggregates suggests them to be potent molecules in overcoming Tau pathology. Further, their origin from a medicinally important plant neem, which known to possess remarkable biological activities was also found to play protective role in HEK293T cells. Basic limonoids were non-toxic to HEK293T cells and also aided in activation of HSF1 by inducing its accumulation in nucleus. Western blotting and immunofluorescence studies showed that HSF1 in downstream increased the transcription of Hsp70 thus, aggravating cytosolic Hsp70 levels that can channel clearance of aberrant Tau. All these results mark basic limonoids as potential therapeutic natural products.

1 aGorantla, Nalini, Vijay1 aDas, Rashmi1 aChidambaram, Hariharakrishnan1 aDubey, Tushar1 aMulani, Fayaj, A.1 aThulasiram, Hirekodathakallu1 aChinnathambi, Subashchandrabose uhttps://library.ncl.res.in/content/basic-limonoid-modulates-chaperone-mediated-proteostasis-and-dissolve-tau-fibrils02335nas a2200265 4500008004100000022001400041245014000055210006900195260000900264300001100273490000800284520138200292653002101674653002601695653002201721653003201743653002101775100002301796700001801819700002201837700002701859700002701886700002501913856013101938 2020 eng d a0016-236100aBifunctional rice husk-derived SiO2-Cu-Al-Mg nanohybrid catalyst for one-pot conversion of biomass-derived furfural to furfuryl acetate0 aBifunctional rice huskderived SiO2CuAlMg nanohybrid catalyst for cSEP  a1179530 v2753 a

Developing one-pot reaction methodologies, which typically require multifunctional catalyst systems, is crucial for sustainable production of bio-derived fuels and chemicals. This work reports one-pot hydrogenation-esterification of furfural to furfuryl acetate using a bifunctional metal-based nanohybrid catalyst, composed of rice husk (RH) derived SiO2, Cu, Al, and Mg species (RHSiO2-Cu-Al-Mg). For comparison, the catalytic efficiency of RHSiO2-Cu and RHSiO2-Cu-Al were tested under similar reaction conditions. Various analytical techniques were used to elucidate the physicochemical, textural, and acid-redox properties of the catalysts. It was found that the RHSiO2-Cu-Al-Mg catalyst contains an optimum amount of acid and redox sites, as illustrated by NH3-TPD and H-2-TPR studies, respectively. Especially, Mg addition played a vital role in tailoring acidity of the RHSiO2-Cu-Al catalyst to promote in-situ esterification of furfuryl alcohol with acetic acid to yield furfuryl acetate. As a result, the RHSiO2-Cu-Al-Mg catalyst exhibited the best performance in one-pot conversion of furfural to furfuryl acetate, outperforming various noble metal/silica based catalysts. This study offers potential opportunities for the rational design of novel, bifunctional heterogeneous catalysts for efficient production of bio-derived fuels and value added chemicals.

10aAcid-redox sites10aBifunctional catalyst10aBio-oil upgrading10aOne-pot furfural conversion10aRice husk silica1 aHashim, Luqman, H.1 aHalilu, Ahmed1 aSudarsanam, Putla1 aUmar, Yahaya, Balarabe1 aBin Johan, Mohd, Rafie1 aBhargava, Suresh, K. uhttps://library.ncl.res.in/content/bifunctional-rice-husk-derived-sio2-cu-al-mg-nanohybrid-catalyst-one-pot-conversion-biomass01108nas a2200277 4500008004100000022001400041245015900055210006900214260000900283300001400292490000600306520012200312653002100434653002600455653001500481653003000496653002700526100002400553700002000577700001600597700001900613700002200632700002500654700002000679856013100699 2020 eng d a2574-096200aBimetallic palladium nickel nanoparticles anchored on carbon as high-performance electrocatalysts for oxygen reduction and formic acid oxidation reactions0 aBimetallic palladium nickel nanoparticles anchored on carbon as  cSEP  a9285-92950 v33 a

{Herein, we report the synthesis of carbon-supported palladium-nickel electrocatalysts (ECs) (Pd4-xNix/C ECs

10aelectrocatalysts10aFormic Acid Oxidation10afuel cells10aoxygen reduction reaction10apalladium-nickel alloy1 aGoswami, Chiranjita1 aSaikia, Himadri1 aTada, Kohei1 aTanaka, Shingo1 aSudarsanam, Putla1 aBhargava, Suresh, K.1 aBharali, Pankaj uhttps://library.ncl.res.in/content/bimetallic-palladium-nickel-nanoparticles-anchored-carbon-high-performance-electrocatalysts01751nas a2200229 4500008004100000022001400041245012000055210006900175260000800244300001200252490000700264520096000271653001901231653001701250653001301267653002701280653002201307100002201329700002601351700001601377856012801393 2020 eng d a0095-978200aBinary mixtures of aprotic and protic ionic liquids demonstrate synergistic polarity effect: an unusual observation0 aBinary mixtures of aprotic and protic ionic liquids demonstrate  cFEB a210-2210 v493 a

In this communication, we demonstrate the solute-solvent and solvent-solvent interactions in the binary mixtures of two aprotic ionic liquids, namely 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide and 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide, with the protic ionic liquid 1-methylimidazolium acetate. The synergistic effects as expressed by the solvatochromic parameter are noted. This observation is in contrast to the mixing of protic ionic liquids 1-methylpyrrolidium acetate and 4-methylmorpholine acetate with 1-methylimidazolium acetate, respectively. It appears that the synergistic effects in the binary mixtures of aprotic and protic ionic liquids are caused by the formation of hydrogen bonds, since cations are dominant H-bond donors while anions are dominant H-bond acceptors. Preferential solvation models are used to describe the solute-solvent interactions in the binary ionic liquid mixtures.

10abinary mixture10aIonic liquid10apolarity10aPreferential solvation10asynergetic effect1 aThawarkar, Sachin1 aKhupse, Nageshwar, D.1 aKumar, Anil uhttps://library.ncl.res.in/content/binary-mixtures-aprotic-and-protic-ionic-liquids-demonstrate-synergistic-polarity-effect02720nas a2200289 4500008004100000022001400041245007600055210006900131260000800200300001100208490000800219520178800227653002702015653002202042653002102064653001202085653001902097653002202116100003502138700002102173700002502194700002902219700003302248700001802281700002702299856010402326 2020 eng d a0928-493100aBiofilm formation to inhibition: role of zinc oxide-based nanoparticles0 aBiofilm formation to inhibition role of zinc oxidebased nanopart cMAR a1103190 v1083 a

Zinc oxide nanoparticles have received much attention worldwide as they possess unique properties like varied morphology, large surface area to volume ratio, potent antibacterial activity, and biocompatibility. Biofilm contains homogenous or heterogeneous microorganisms that remain enclosed in a matrix of an extracellular polymeric substance on biotic or abiotic surfaces. Bacterial biofilm formed on medical devices such as central venous catheters, urinary catheters, prosthetic joints, cardiovascular implantable devices, dental implants, contact lenses, intrauterine contraceptive devices and breast implants cause persistent infections. Such biofilm-associated infections in medical implants cause serious problems for public health and affect the function of medical implants. So, there is an urgent need for the use of an antimicrobial agent that will inhibit biofilm, including such antibiotic-resistant bacterial strains as bacteria, to develop multiple drug-resistances resulting in failure of the antibiotic's action. The antimicrobial agent used should be ideal in terms of biocompatibility, antimicrobial activity, stability at different environmental conditions, with less sensitivity to the development of resistance towards micro-organisms, safe for in vivo and in vitro use, and remain non-hazardous to the environment, etc. The first objective of the review discusses the insights into the formation of biofilm on a medical device with the current strategies to inhibit. The second purpose is to review the recent progress in ZnO- based nanostructure including composites for antibacterial and anti-biofilm activities. This will offer a new opportunity for the application of Zinc oxide-based material in the prevention of biofilm on the medical devices.

10aantibacterial activity10aAntibiofilm agent10aBiocompatibility10aBiofilm10aNanocomposites10aZnO nanoparticles1 aMahamuni-Badiger, Pranjali, P.1 aPatil, Pooja, M.1 aBadiger, Manohar, V.1 aPatel, Pratikshkumar, R.1 aThorat-Gadgil, Bhagyashi, S.1 aPandit, Abhay1 aBohara, Raghvendra, A. uhttps://library.ncl.res.in/content/biofilm-formation-inhibition-role-zinc-oxide-based-nanoparticles02820nas a2200349 4500008004100000022001400041245014900055210006900204260000800273300001100281490000700292520170300299653001102002653001802013653002302031653002402054653003002078100001802108700001902126700001802145700001802163700002002181700001402201700001602215700001902231700001902250700002002269700001602289700001902305700002102324856012502345 2020 eng d a2468-519400aBioinspired synthesis of multifunctional silver nanoparticles for enhanced antimicrobial and catalytic applications with tailored SPR properties0 aBioinspired synthesis of multifunctional silver nanoparticles fo cSEP a1002850 v173 a

In the developing nanotechnology world, numerous attempts have been made to prepare the nobel metallic nanoparticles (NPs), which can improve their applicability in diverse fields. In the present work, the biosynthesis of silver (Ag) NPs has been successfully achieved through the medicinal plant extract (PE) of G. resinifera and effectively used for the catalytic and antibacterial applications. The size dependant tuneable surface plasmon resonance (SPR) properties attained through altering precursor concentrations. The X-ray and selected area diffraction pattern for Ag NPs revealed the high crystalline nature of pure Ag NPs with dominant (111) phase. The high-resolution TEM images show the nonspherical shape of NPs shifting from spherical, hexagonal to triangular, with wide particle size distribution ranging from 13 to 44 nm. Accordingly, the dual-band SPR spectrum is situated in the UV-Vis spectra validating the non-spherical shape of Ag NPs. The functional group present on the Ag NPs surface was analysed by FT-IR confirms the capping and reducing ability of methanolic PE G. resinifera. Further, the mechanism of antimicrobial activity studied using electron microscope showed the morphological changes with destructed cell walls of E. coli NCIM 2931 and S. aureus NCIM 5021 cells, when they treated with Ag NPs. The Ag NPs were more effective against S. aureus and E. coli with MIC 128 mu g/ml as compared to P. aeruginosa NCIM 5029 with MIC 256 mu g/ml. Apart from this, the reduction of toxic organic pollutant 4-NP to 4-AP within 20 min reveals the excellent catalytic activity of Ag NPs with rate constant k = 15.69 s(-1). (C) 2020 Elsevier Ltd. All rights reserved.

10aAg NPs10aAntibacterial10aCatalytic activity10aGardenia resinifera10asurface plasmon resonance1 aParit, S., B.1 aKarade, V., C.1 aPatil, R., B.1 aPawar, V., N.1 aDhavale, R., P.1 aTawre, M.1 aPardesi, K.1 aJadhav, U., U.1 aDawkar, V., V.1 aTanpure, R., S.1 aKim, J., H.1 aJadhav, J., P.1 aChougale, A., D. uhttps://library.ncl.res.in/content/bioinspired-synthesis-multifunctional-silver-nanoparticles-enhanced-antimicrobial-and01692nas a2200253 4500008004100000022001400041245011600055210006900171260000800240300001400248490000700262520086000269653000601129653000801135653002401143653003001167653001201197653001601209100002001225700002001245700002301265700002101288856012901309 2020 eng d a1735-147200aBiomass ashes as potent adsorbent for pesticide: prediction of adsorption capacity by artificial neural network0 aBiomass ashes as potent adsorbent for pesticide prediction of ad cJUN a3209-32160 v173 a

Biomass ashes are used for adsorption of herbicides from aqueous solution. A relationship between physicochemical properties of biomass ashes such as carbon-hydrogen-nitrogen content (CHN analysis), silica content and BET surface area with their adsorption capacity was established and modeled using artificial neural network. 2,4-Dichlorophenoxyacetic acid (2,4-D) a commonly used herbicide is chosen a representative for this study. The artificial neural network model was trained, validated and tested using 35 data sets and was equipped with nine neuron hidden layers having tansig (tangent sigmoid) transfer function and an output layer with purelin (purely linear) transfer function. This model can be used to predict 2,4-D removal efficacy of any biomass ash by knowing its physicochemical properties like C, H, N, Si and BET surface area.

10a210a4-D10aAdsorption capacity10aArtificial neural network10aBiochar10abiomass ash1 aGokhale, N., A.1 aTrivedi, N., S.1 aMandavgane, S., A.1 aKulkarni, B., D. uhttps://library.ncl.res.in/content/biomass-ashes-potent-adsorbent-pesticide-prediction-adsorption-capacity-artificial-neural02195nas a2200217 4500008004100000022001400041245005700055210005500112260000800167300000800175490000700183520157300190653001501763653001301778653002001791653001501811100001901826700002401845700002301869856008501892 2020 eng d a2190-572X00aBiosensors: frontiers in rapid detection of COVID-190 aBiosensors frontiers in rapid detection of COVID19 cAUG a3850 v103 a

The rapid community-spread of novel human coronavirus 2019 (nCOVID19 or SARS-Cov2) and morbidity statistics has put forth an unprecedented urge for rapid diagnostics for quick and sensitive detection followed by contact tracing and containment strategies, especially when no vaccine or therapeutics are known. Currently, quantitative real-time polymerase chain reaction (qRT-PCR) is being used widely to detect COVID-19 from various types of biological specimens, which is time-consuming, labor-intensive and may not be rapidly deployable in remote or resource-limited settings. This might lead to hindrance in acquiring realistic data of infectivity and community spread of SARS-CoV-2 in the population. This review summarizes the existing status of current diagnostic methods, their possible limitations, and the advantages of biosensor-based diagnostics over the conventional ones for the detection of SARS-Cov-2. Novel biosensors used to detect RNA-viruses include CRISPR-Cas9 based paper strip, nucleic-acid based, aptamer-based, antigen-Au/Ag nanoparticles-based electrochemical biosensor, optical biosensor, and Surface Plasmon Resonance. These could be effective tools for rapid, authentic, portable, and more promising diagnosis in the current pandemic that has affected the world economies and humanity. Present challenges and future perspectives of developing robust biosensors devices for rapid, scalable, and sensitive detection and management of COVID-19 are presented in light of the test-test-test theme of the World Health Organization (WHO).

10aBiosensors10aCOVID-1910aRapid detection10aSARS-CoV-21 aSamson, Rachel1 aNavale, Govinda, R.1 aDharne, Mahesh, S. uhttps://library.ncl.res.in/content/biosensors-frontiers-rapid-detection-covid-1901617nas a2200193 4500008004100000022001400041245014700055210006900202260000800271300001200279490000700291520087500298100002101173700002301194700002901217700002601246700002301272856012801295 2020 eng d a0340-428500aBio-surfactant: a green and environmentally benign reaction medium for ligand-free Pd-catalyzed Mizoroki-Heck cross-coupling reaction in water0 aBiosurfactant a green and environmentally benign reaction medium cSEP a403-4110 v453 a

A simple and efficient protocol for the ligand-free Mizoroki-Heck coupling reaction of various aryl bromides with different olefins has been reported by using in situ generated PdNPs of size 5-10 nm in aqueous solution of bio-surfactant. The bio-surfactant used in this study is a saponin extract of the seeds of pericarps (pods) of theAcacia concinnaplant. The in situ generated PdNPs have been characterized by various techniques such as HRTEM, EDS and XPS. The influence of various parameters such as the nature and amount of bases, the nature of Pd precatalysts as well as the effect of temperature has been investigated on Mizoroki-Heck coupling reaction. The generated PdNPs significantly coupled the various aryl bromides with different olefins in aqueous extract of the seeds of pericarps (pods) of theAcacia concinnaplant at 100 degrees C. [GRAPHICS] .

1 aPatil, Seema, P.1 aJadhav, Sanjay, N.1 aRode, Chandrashekhar, V.1 aShejwal, Rajendra, V.1 aKumbhar, Arjun, S. uhttps://library.ncl.res.in/content/bio-surfactant-green-and-environmentally-benign-reaction-medium-ligand-free-pd-catalyzed02560nas a2200313 4500008004100000022001400041245010100055210006900156260000800225300001100233490000800244520153800252653002601790653001201816653001201828653003301840653001401873653002801887653001701915653002901932653002001961100002001981700002502001700001902026700001802045700003302063700002002096856013002116 2020 eng d a0031-942200aBiosynthesis and tissue-specific partitioning of camphor and eugenol in Ocimum kilimandscharicum0 aBiosynthesis and tissuespecific partitioning of camphor and euge cSEP a1124510 v1773 a

In Ocimum kilimandscharicum, the relative volatile composition of camphor in leaves was as high as 55%, while that of eugenol in roots was 57%. These metabolites were differentially partitioned between the aerial and root tissues. Global metabolomics revealed tissue-specific biochemical specialization, evident by the differential distribution of 2588 putative metabolites across nine tissues. Next-generation sequencing analysis indicated differential expression of 51 phenylpropanoid and 55 terpenoid pathway genes in aerial and root tissues. By integrating metabolomics with transcriptomics, the camphor biosynthesis pathway in O. kilimandscharicum was elucidated. In planta bioassays revealed the role of geranyl diphosphate synthase (gpps) and borneol dehydrogenase (bdh) in camphor biosynthesis. Further, the partitioning of camphor was attributed to tissue-specific gene expression of both the pathway entry point (gpps) and terminal (bdh) enzyme. Unlike camphor, eugenol accumulated more in roots; however, absence of the eugenol synthase gene in roots indicated long distance transport from aerial tissues. In silico co-expression analysis indicated the potential involvement of ATP-binding cassette, multidrug and toxic compound extrusion, and sugar transporters in eugenol transport. Similar partitioning was evident across five other Ocimum species. Overall, our work indicates that metabolite partitioning maybe a finely regulated process, which may have implications on plant growth, development, and defense.

10aBorneol dehydrogenase10aCamphor10aEugenol10aGeranyl diphosphate synthase10aLamiaceae10aMetabolite partitioning10aMetabolomics10aOcimum kilimandscharicum10aTranscriptomics1 aSingh, Priyanka1 aKalunke, Raviraj, M.1 aShukla, Anurag1 aTzfadia, Oren1 aThulasiram, Hirekodathakallu1 aGiri, Ashok, P. uhttps://library.ncl.res.in/content/biosynthesis-and-tissue-specific-partitioning-camphor-and-eugenol-ocimum-kilimandscharicum01423nas a2200193 4500008004100000022001400041245008200055210006900137260000900206300001600215490000700231520078600238100002101024700002201045700001901067700001501086700001801101856011001119 2020 eng d a0020-166900aBis(silanetellurone) with C-H center dot center dot center dot Te Interaction0 aBissilanetellurone with CH center dot center dot center dot Te I cDEC  a17811-178210 v593 a

Herein, we report the synthesis of a series of bis(silanechalcogenones) [Ch = Te (2), S (3), or Se (4)] using an N-heterocyclic silylene-based SiCSi pincer ligand (1). 2 is the first example of a bis(silanetellurone) derivative. The bonding patterns of 2-4 were extensively studied by natural bond orbital, quantum theory of atoms in molecules, and noncovalent interaction index analyses, and these exhibit weak C-H center dot center dot center dot Ch interaction. The analogous reaction of 1 with trimethyl N-oxide produced a novel bis(cyclosiloxane) derivative (5). All of the complexes are duly characterized by single-crystal X-ray diffraction studies, multinuclear nuclear magnetic resonance (H-1, C-13, and Si-29) spectroscopy, and high-resolution mass spectrometry.

1 aGhosh, Moushakhi1 aPanwaria, Prakash1 aTothadi, Srinu1 aDas, Aloke1 aKhan, Shabana uhttps://library.ncl.res.in/content/bissilanetellurone-c-h-center-dot-center-dot-center-dot-te-interaction02551nas a2200181 4500008004100000245008300041210006900124260000900193300001600202490000700218520191800225100002302143700001802166700002102184700002602205700002902231856010902260 2020 eng d00aBlends of neem oil based polyesteramide as nanofiber mats to control Culicidae0 aBlends of neem oil based polyesteramide as nanofiber mats to con cNOV  a42827-428370 v103 a

Mosquitoes act as vectors for several disease-causing microorganisms and pose a threat to mankind by transmitting various diseases. There are different conventional methods to repel or kill these mosquitoes for avoiding susceptibility against infections. However, to overcome the difficulties with conventional methods, new advanced materials are being studied. For the first time, we report developing a nanofiber mat with a controlled release of insecticide to repel or detain the mosquitoes. Briefly, various blend compositions were prepared by manipulating the ratio of neem oil-based polyesteramide (PEA) and polycaprolactone (PCL) immobilized with insecticide, transfluthrin (Tf). The blend solutions were electrospun to get non-woven nanofiber mats, and these nanomaterials were characterized by various spectroscopic techniques to understand their physicochemical properties. The surface morphology was analyzed using environmental scanning electron microscopy (E-SEM), and the diameter of the nanofibers was in the range of 200 to 450 nm. Further, thermal and mechanical properties were evaluated to understand the stability of nanofiber mats. In vitro drug release studies of nanofiber mat PPT-1335 showed controlled and sustained release of Tf, with similar to 35% of Tf released in 24 h. However, a film of the same composition (PPT-1335) showed similar to 5% of Tf release within 24 h. Moreover, in vivo bio-efficacy studies suggested the mortality of mosquitoes was about 50% with PP-133, which was further increased to 100% within 12 h in the presence of Tf (PPT-1335). However, 60% mortality of mosquitoes was observed with the film of PPT-1335. Hence, the nanofiber mat showed better efficacy against mosquitoes as compared to the film of the same composition. The degradation studies under various conditions revealed biocompatibility of the developed nanofiber mats with the ecosystem.

1 aKonchada, Sravanya1 aKilli, Naresh1 aSayyad, Shahebaz1 aGathalkar, Ganesh, B.1 aGundloori, Rathna, V. N. uhttps://library.ncl.res.in/content/blends-neem-oil-based-polyesteramide-nanofiber-mats-control-culicidae02288nas a2200325 4500008004100000022001400041245012100055210006900176260000900245300001600254490000600270520118200276653002401458653002801482653001901510653002101529653002801550653002201578653003701600100002201637700002601659700001601685700002401701700002401725700002001749700002801769700001901797700002401816856012201840 2020 eng d a2168-048500aBoron nitride and oxide supported on dendritic fibrous nanosilica for catalytic oxidative dehydrogenation of propane0 aBoron nitride and oxide supported on dendritic fibrous nanosilic cNOV  a16124-161350 v83 a

In this work, we were able to significantly increase the activity of boron nitride (BN) catalysts used for the oxidative dehydrogenation (ODH) of propane by designing and synthesizing BN supported on dendritic fibrous nanosilica (DFNS). DFNS/BN showed a markedly increased catalytic efficiency, accompanied by exceptional stability and selectivity. Textural characterization, together with solid-state nuclear magnetic resonance (NMR) and X-ray photoelectron spectroscopic analyses, indicates the presence of a combination of unique fibrous morphology of DFNS and various boron sites connected to silica to be the reason for this increase in the catalytic performance. Notably, DFNS/B2O3 also showed catalytic activity, although with more moderate selectivity compared to that of DFNS/BN. Solid-state NMR spectra indicate that the higher selectivity of DFNS/BN might stem from a larger amount of hydrogen-bonded hydroxyl groups attached to B atoms. This study indicates that both boron nitride and oxide are active catalysts and by using high surface area support (DFNS), conversion from propane to propene as well as productivity of olefins was significantly increased.

10aElectron microscopy10aheterogeneous catalysis10aNanostructures10aNMR spectroscopy10apropane dehydrogenation10aX-ray diffraction10aX-ray photoelectron spectroscopy1 aBelgamwar, Rajesh1 aRankin, Andrew, G. M.1 aMaity, Ayan1 aMishra, Amit, Kumar1 aGomez, Jennifer, S.1 aTrebosc, Julien1 aVinod, Chathakudath, P.1 aLafon, Olivier1 aPolshettiwar, Vivek uhttps://library.ncl.res.in/content/boron-nitride-and-oxide-supported-dendritic-fibrous-nanosilica-catalytic-oxidative02930nas a2200217 4500008004100000245010400041210006900145260000900214300000700223490000700230520216300237653002402400653001402424653002002438653001902458653001802477100002902495700002602524700003602550856012602586 2021 eng d00aBaicalein inhibits heparin-induced Tau aggregation by initializing non-toxic Tau oligomer formation0 aBaicalein inhibits heparininduced Tau aggregation by initializin cFEB  a160 v193 a

Background: Amyloid aggregate deposition is the key feature of Alzheimer's disease. The proteinaceous aggregates found in the afflicted brain are the intra-neuronal neurofibrillary tangles formed by the microtubule-associated protein Tau and extracellular deposits, senile plaques, of amyloid beta (A beta) peptide proteolytically derived from the amyloid precursor protein. Accumulation of these aggregates has manifestations in the later stages of the disease, such as memory loss and cognitive inabilities originating from the neuronal dysfunction, neurodegeneration, and brain atrophy. Treatment of this disease at the late stages is difficult, and many clinical trials have failed. Hence, the goal is to find means capable of preventing the aggregation of these intrinsically disordered proteins by inhibiting the early stages of their pathological transformations. Polyphenols are known to be neuroprotective agents with the noticeable potential against many neurodegenerative diseases, such as Alzheimer's, Parkinson's, and Prion diseases. Methods: We analyzed the capability of Baicalein to inhibit aggregation of human Tau protein by a multifactorial analysis that included several biophysical and biochemical techniques. Results: The potency of Baicalein, a polyphenol from the Scutellaria baicalensis Georgi, against in vitro Tau aggregation and PHF dissolution has been screened and validated. ThS fluorescence assay revealed the potent inhibitory activity of Baicalein, whereas ANS revealed its mechanism of Tau inhibition viz. by oligomer capture and dissociation. In addition, Baicalein dissolved the preformed mature fibrils of Tau thereby possessing a dual target action. Tau oligomers formed by Baicalein were non-toxic to neuronal cells, highlighting its role as a potent molecule to be screened against AD. Conclusion: In conclusion, Baicalein inhibits aggregation of hTau40 by enhancing the formation of SDS-stable oligomers and preventing fibril formation. Baicalein-induced oligomers do not affect the viability of the neuroblastoma cells. Therefore, Baicalein can be considered as a lead molecule against Tau pathology in AD.

10aAlzheimer's disease10aBaicalein10aTau dissolution10aTau inhibition10aTau Oligomers1 aSonawane, Shweta, Kishor1 aUversky, Vladimir, N.1 aChinnathambi, Subashchandrabose uhttps://library.ncl.res.in/content/baicalein-inhibits-heparin-induced-tau-aggregation-initializing-non-toxic-tau-oligomer01090nas a2200193 4500008004100000245011900041210006900160260000800229520044200237100002500679700001800704700002100722700001700743700002100760700001900781700002000800700002300820856005300843 2021 eng d00aBibliometric analysis of emerging technologies in the field of computer science helping in ovarian cancer research0 aBibliometric analysis of emerging technologies in the field of c cAPR3 aThis study is carried out to provide an analysis of the literature available at the intersection of ovarian cancer and computing. A comprehensive search was conducted using Scopus database for English-language peer-reviewed articles. The study administers chronological, domain clustering and text analysis of the articles under consideration to provide high-level concept map composed of specific words and the connections between them.1 aKothari, Dr., Sonali1 aGupta, Anvita1 aAgrawal, Muskaan1 aJaggi, Kajal1 aGoswami, Adhiraj1 aKotecha, Ketan1 aDr, Karthikeyan1 aKhedkar, Vijayhsri uhttps://digitalcommons.unl.edu/libphilprac/5590/01927nas a2200181 4500008004100000245013100041210006900172260000800241520131000249100002301559700002501582700001801607700001801625700001501643700001401658700002001672856005301692 2021 eng d00aBibliometric analysis of named entity recognition for chemoinformatics and biomedical information extraction of ovarian cancer0 aBibliometric analysis of named entity recognition for chemoinfor cAPR3 aWith the massive amount of data that has been generated in the form of unstructured text documents, Biomedical Named Entity Recognition (BioNER) is becoming increasingly important in the field of biomedical research. Since currently there does not exist any automatic archiving of the obtained results, a lot of this information remains hidden in the textual details and is not easily accessible for further analysis. Hence, text mining methods and natural language processing techniques are used for the extraction of information from such publications.Named entity recognition, is a subtask that comes under information extraction that focuses on finding and categorizing specific entities in text. In this paper, bibliometric analysis of named entity recognition of ovarian cancer is carried out using information about publications from Scopus. The most productive journals, countries and authors are determined. The most frequently cited article and its citation history has been described. Also bibliometric maps based on citation network among countries are constructed. This study can assist people in the medical field to get a comprehensive understanding of the study of BioNER. It can also be utilized for reference works, for the research and application of the BioNER visualization methods. 1 aKhedkar, Vijayhsri1 aFernandes, Charlotte1 adesai, Devshi1 aShinde, Mansi1 aDr, Chavan1 aDr, Tidke1 aDr, Karthikeyan uhttps://digitalcommons.unl.edu/libphilprac/5536/01874nas a2200253 4500008004100000022001400041245016500055210006900220260000900289300000600298490000800304520096100312653002701273653001601300653002701316653001601343653002001359100002101379700002401400700002901424700002201453700001701475856012801492 2021 eng d a0974-362600aBimetallic nano alloy architecture on a special polymer: Ni or Cu merged with Pd for the promotion of the Mizoroki-Heck reaction and the Suzuki-Miyaura coupling0 aBimetallic nano alloy architecture on a special polymer Ni or Cu cFEB  a80 v1333 a

Novel Ni-Pd and Cu-Pd bimetallic nano alloys was designed and heterogenized on the highly robust ABPBI [poly(2,5-benzimidazole)] polymer in high yields using NaBH4 as reducing agent. These were versatile ligand free catalysts for the Mizoroki-Heck reaction and Suzuki-Miyaura coupling. The bimetallic Ni-Pd-ABPBI catalyst for the Mizoroki-Heck reaction of 4-iodo anisole could be recycled 5 times with high yields. Aryl bromides could also be activated for the Mizoroki-Heck reaction using Cu-Pd-ABPBI NP catalysts, with moderate yields. Graphic abstractSynopsis Novel bimetallic Ni-Pd and Cu-Pd nano alloys, heterogenized on the robust ABPBI [poly(2,5-benzimidazole)] polymer using NaBH4 as reducing agent, is described. These were versatile ligand free, noble metal conservative catalysts, for the Mizoroki-Heck reaction and the Suzuki-Miyaura coupling. Aryl bromides were activated for the Mizoroki-Heck reaction using the Cu-Pd-ABPBI catalyst.

10aBimetallic nano alloys10aCu-Pd-ABPBI10aMizoroki-Heck reaction10aNi-Pd-ABPBI10aSuzuki coupling1 aPatil, Vijay, P.1 aKashid, Abhijit, A.1 aSolanki, Bhanupratap, S.1 aKharul, Ulhas, K.1 aIyer, Suresh uhttps://library.ncl.res.in/content/bimetallic-nano-alloy-architecture-special-polymer-ni-or-cu-merged-pd-promotion-mizoroki01477nas a2200289 4500008004100000022001400041245013200055210006900187260000800256300001200264490000800276520049900284653002500783653001800808653002100826653001300847653002000860100002400880700002500904700002200929700002100951700001900972700002400991700002501015700001701040856013001057 2021 eng d a1011-372X00aBimetallic Ni-Pd synergism-mixed metal catalysis of the mizoroki-heck reaction and the suzuki-miyaura coupling of aryl bromides0 aBimetallic NiPd synergismmixed metal catalysis of the mizorokihe cFEB a353-3580 v1513 a

A combination of Pd and Ni complexes activated aryl bromides for the thermal Mizoroki-Heck reaction and Suzuki coupling giving high yields in short reaction times. A thermal redox mechanism probably occurs whereby Ni complex transfers electron and reduces the Pd (II) to Pd (0) which then takes the reactants through the standard protocol of oxidative-addition, migratory insertion and reductive elimination, typical for the Mizoroki-Heck reaction and the Suzuki coupling. [GRAPHICS] .

10aBimetallic catalysis10aMizoroki-Heck10aNickel complexes10aPd(OAc)210aSuzuki coupling1 aKashid, Abhijit, A.1 aPatil, Dharmaraj, J.1 aMali, Ramling, D.1 aPatil, Vijay, P.1 aNeethu, T., V.1 aMeroliya, Heena, K.1 aWaghmode, Shobha, A.1 aIyer, Suresh uhttps://library.ncl.res.in/content/bimetallic-ni-pd-synergism-mixed-metal-catalysis-mizoroki-heck-reaction-and-suzuki-miyaura01334nas a2200229 4500008004100000020001400041245013800055210007300193260000800266300001400274490000800288520047700296100002400773700002500797700002200822700002100844700001900865700002400884700002500908700001700933856015400950 2021 eng d a1572-879X00aBimetallic Ni–Pd synergism—mixed metal catalysis of the mizoroki-heck reaction and the suzuki–miyaura coupling of aryl bromides0 aBimetallic Ni–Pd synergism—mixed metal catalysis of the mizoroki cFEB a353 - 3580 v1513 aA combination of Pd and Ni complexes activated aryl bromides for the thermal Mizoroki-Heck reaction and Suzuki coupling giving high yields in short reaction times. A thermal redox mechanism probably occurs whereby Ni complex transfers electron and reduces the Pd (II) to Pd (0) which then takes the reactants through the standard protocol of oxidative-addition, migratory insertion and reductive elimination, typical for the Mizoroki-Heck reaction and the Suzuki coupling.1 aKashid, Abhijit, A.1 aPatil, Dharmaraj, J.1 aMali, Ramling, D.1 aPatil, Vijay, P.1 aNeethu, T., V.1 aMeroliya, Heena, K.1 aWaghmode, Shobha, A.1 aIyer, Suresh uhttps://library.ncl.res.in/content/bimetallic-ni%E2%80%93pd-synergism%E2%80%94mixed-metal-catalysis-mizoroki-heck-reaction-and-suzuki%E2%80%93miyaura01831nas a2200265 4500008004100000022001400041245012300055210006900178260000900247300001100256490000800267520095300275653001501228653002201243653002601265653001901291653002201310100001901332700001901351700001801370700001601388700001801404700001801422856012501440 2021 eng d a0169-433200aBimetallic ZnFe2O4 nanosheets prepared via electrodeposition as binder-free high-performance supercapacitor electrodes0 aBimetallic ZnFe2O4 nanosheets prepared via electrodeposition as  cSEP  a1499510 v5593 a

Binder-free bimetallic ZnFe2O4 nanosheets were fabricated using one-step electrodeposition, which facilitated excellent electrical contact between the ZnFe2O4 nanosheets and the nickel substrate. The resultant numerous ZnFe2O4 nanosheets with their cubic spinel structures promote interfacial activity to enhance the electrochemical and Faradaic redox reactions. The metallic Zn and Fe from the cubic spinel structure of ZnFe2O4 attract electrolytic ions and increase the energy-storage capability, thus yielding a specific capacitance of 1093 F.g(-1) at a current rate of 1 A.g(-1). Different samples were prepared by varying the amount of metal salts in the electrodeposition solution while maintaining a constant ZnNt:FeSO4 concentration ratio of 1:2 for all cases. The optimal electrode composition, which yielded an energy density of 54 Wh.kg(-1) and a capacitance retention of 93.5% at N = 5000 charge-discharge cycles, was identified.

10aBimetallic10aElectrodeposition10aEnergy storage device10asupercapacitor10aZnFe2O4 nanosheet1 aJoshi, Bhavana1 aSamuel, Edmund1 aPark, Chanwoo1 aKim, Yongil1 aLee, Hae-Seok1 aYoon, Sam, S. uhttps://library.ncl.res.in/content/bimetallic-znfe2o4-nanosheets-prepared-electrodeposition-binder-free-high-performance02064nas a2200265 4500008004100000022001400041245011200055210006900167260000800236300001400244490000700258520120900265653000601474653001701480653001701497653002101514653001301535653001301548653002701561100001801588700002001606700001901626700002501645856012801670 2021 eng d a0268-257500aBioglycerol (C3) upgrading to 2,3-butanediol (C4) by cell-free extracts of Enterobacter aerogenes NCIM 26950 aBioglycerol C3 upgrading to 23butanediol C4 by cellfree extracts cMAY a1316-13250 v963 a

BACKGROUND Production of biobased chemicals from renewable resources is a green starring approach that serves as a substitute to petroleum derivatives. Bioglycerol, with its growing production as a co-product of biodiesel, is an attractive low-cost feedstock for the synthesis of platform chemicals by microbial fermentation. 2,3-butanediol (2,3-BDO) is amongst the top biorefinery platform chemicals that can be produced by glycerol fermentation. RESULTS The `Circular Economy' concept is demonstrated by converting the by-product bioglycerol using a cell-free extract of Enterobacter aerogenes NCIM 2695 (National Collection of Industrial Microorganisms, NCIM), yielding 22 g L-1 2,3-BDO, in 96 h, 98% atom economy and 0.4 g/g E factor. The cell-free bioglycerol conversion to 2,3-BDO was proved using a modified procedure for determining glycerol dehydrogenase enzyme assay by protein analysis and it was also shown to be cell-bounded. CONCLUSION Our study offers an effective utilization of the leftover material (i.e. cell-free extract) that biocatalysed C3 to C4 diol, which adds value to the overall economics of the process using only crude glycerol (C3) itself as a fermentative medium.

10a210a3-Butanediol10aatom economy10acircular economy10aE factor10aGlycerol10aglycerol dehydrogenase1 aParate, Roopa1 aBorgave, Mrunal1 aDharne, Mahesh1 aRode, Chandrashekhar uhttps://library.ncl.res.in/content/bioglycerol-c3-upgrading-23-butanediol-c4-cell-free-extracts-enterobacter-aerogenes-ncim03127nas a2200409 4500008004100000022001400041245009300055210006900148260000900217300001200226490000700238520193200245653001602177653001702193653001102210653002602221653001702247653001202264100001602276700002102292700001402313700001402327700001602341700001602357700001702373700001502390700001502405700001602420700003002436700002102466700002902487700003002516700002102546700001502567700001602582856011902598 2021 eng d a1478-322300aBiological process of lysine-tRNA charging is therapeutically targetable in liver cancer0 aBiological process of lysinetRNA charging is therapeutically tar cJAN  a206-2190 v413 a

Background & Aims Mature transfer RNAs (tRNA) charged with amino acids decode mRNA to synthesize proteins. Dysregulation of translational machineries has a fundamental impact on cancer biology. This study aims to map the tRNAome landscape in liver cancer patients and to explore potential therapeutic targets at the interface of charging amino acid with tRNA. Methods Resected tumour and paired tumour-free (TFL) tissues from hepatocellular carcinoma (HCC) patients (n = 69), and healthy liver tissues from organ transplant donors (n = 21), HCC cell lines, and cholangiocarcinoma (CC) patient-derived tumour organoids were used. Results The expression levels of different mature tRNAs were highly correlated and closely clustered within individual tissues, suggesting that different members of the tRNAome function cooperatively in protein translation. Interestingly, high expression of tRNA-Lys-CUU in HCC tumours was associated with more tumour recurrence (HR 1.1;P = .022) and worse patient survival (HR 1.1;P = .0037). The expression of Lysyl-tRNA Synthetase (KARS), the enzyme catalysing the charge of lysine to tRNA-Lys-CUU, was significantly upregulated in HCC tumour tissues compared to tumour-free liver tissues. In HCC cell lines, lysine deprivation, KARS knockdown or treatment with the KARS inhibitor cladosporin effectively inhibited overall cell growth, single cell-based colony formation and cell migration. This was mechanistically mediated by cell cycling arrest and induction of apoptosis. Finally, these inhibitory effects were confirmed in 3D cultured patient-derived CC organoids. Conclusions The biological process of charging tRNA-Lys-CUU with lysine sustains liver cancer cell growth and migration, and is clinically relevant in HCC patients. This process can be therapeutically targeted and represents an unexplored territory for developing novel treatment strategies against liver cancer.

10aCladosporin10aliver cancer10alysine10aLysyl-tRNA Synthetase10atRNA-Lys-CUU10atRNAome1 aZhang, Ruyi1 aNoordam, Lisanne1 aOu, Xumin1 aMa, Buyun1 aLi, Yunlong1 aDas, Pronay1 aShi, Shaojun1 aLiu, Jiaye1 aWang, Ling1 aLi, Pengfei1 aVerstegen, Monique, M. A.1 aReddy, Srinivasa1 avan Der Laan, Luc, J. W.1 aPeppelenbosch, Maikel, P.1 aKwekkeboom, Jaap1 aSmits, Ron1 aPan, Qiuwei uhttps://library.ncl.res.in/content/biological-process-lysine-trna-charging-therapeutically-targetable-liver-cancer02847nas a2200289 4500008004100000022001400041245012500055210006900180260000800249300001100257490000700268520189300275653001702168653001102185653001402196653001502210653003102225100001502256700003102271700002102302700002702323700002402350700001702374700002102391700001702412856012802429 2021 eng d a1773-224700aBiological synthesis of alpha-Ag2S composite nanoparticles using the fungus Humicola sp. and its biomedical applications0 aBiological synthesis of alphaAg2S composite nanoparticles using  cDEC a1027700 v663 aSynthesis of alpha-Ag2S (silver sulfide) is a highly challenging task, usually attempted using synthetic, hazardous chemical processes. This report puts efforts to exploring microbial-based eco-friendly alternatives. A facile, onepot biological method for the synthesis of alpha-Ag2S composite nanoparticles (NPs) in an aqueous medium at ambient conditions was established. As prepared alpha-Ag2S composite NPs were efficiently produced from precursors silver nitrate and sodium sulfite. The extracellular biomolecules produced by Humicola sp. reduced the precursor solution and at the same time it capped the formed Ag2S NPs. The characterization performed by different techniques revealed excellent optical, morphological, and structural properties (size, shape distribution, and crystallinity) of the alpha-Ag2S NPs. The change in color from colorless to dark brown indicated the formation of alpha-Ag2S, which was further confirmed by fluorescence and UV-Visible spectroscopy, showing a sharp peak at 410 nm. Morphological analysis of alpha-Ag2S NPs confirmed the spherical shape, and narrow size distribution. alpha-Ag2S NPs were found to be potent in antimicrobial applications owing to their high dispersibility in water and capping by extracellular biomolecules secreted by Humicola sp. The anti-cancer activity for alpha-Ag2S performed using human breast cancer cell line (ZR-75-1) and human Burkitt's lymphoma cancer (Daudi). The biocompatibility test was performed using human peripheral blood mononuclear cells (PBMCs) for alpha-Ag2S NPs. The anti-leishmania activity was also determined for alpha Ag2S NPs in-vitro using the standard anti-promastigote protocol (Leishmania donovani promastigotes). This extracellular system is an economically and environmentally benign route for the biosynthesis of alpha-Ag2S NPs having desired surface, structure and biomedical properties.10aCytotoxicity10aFungus10aHemolysis10aHumicolasp10aSilver sulfide(alpha-Ag2S)1 aSyed, Asad1 aSaedi, Marzouq, Hussain Al1 aBahkali, Ali, H.1 aElgorban, Abdallah, M.1 aKharat, Maheshkumar1 aPai, Kalpana1 aGhodake, Gajanan1 aAhmad, Absar uhttps://library.ncl.res.in/content/biological-synthesis-alpha-ag2s-composite-nanoparticles-using-fungus-humicola-sp-and-its02005nas a2200253 4500008004100000022001400041245016400055210006900219260000800288300001100296490000700307520111200314653001201426653002001438653002001458653002301478653001901501100001701520700001701537700002201554700002401576700002201600856012901622 2021 eng d a2352-152X00aBiomass-derived activated carbon material from native European deciduous trees as an inexpensive and sustainable energy material for supercapacitor application0 aBiomassderived activated carbon material from native European de cFEB a1021780 v343 a

Activated carbons are one of the possible electrode materials for supercapacitors (SCs), which are widely used in commercial applications. Herein, we reported the synthesis of a novel activated carbon derived through a cavitation process from the mixture of native European deciduous trees, Birch, Fagaceae, and Carpinus betulus (commonly known as European hornbeam), which was employed as the electrode material in SC. From the morphological and structural characterization, we observed that the prepared sample is a desirable carbon with good porosity and high specific surface area of about 614 m(2) g(-1). The electrochemical properties of the synthesized material were evaluated with a three-electrode configuration in 1.0 M H2SO4 electrolyte. It was found that in device mode, the carbon material delivers a specific capacitance of 24 F g(-1) at 0.25 A g(-1) with excellent cycling stability of over 10000 consecutive charge/discharge cycles. Thus, our studies demonstrate the facile synthesis of biomass-derived carbon and its application as a versatile electrode material for SC applications.

10aBiomass10aCarbon material10aDeciduous trees10aElectrode material10asupercapacitor1 aJain, Amrita1 aGhosh, Meena1 aKrajewski, Marcin1 aKurungot, Sreekumar1 aMichalska, Monika uhttps://library.ncl.res.in/content/biomass-derived-activated-carbon-material-native-european-deciduous-trees-inexpensive-and01176nas a2200169 4500008004100000022001400041245014500055210006900200260000900269300001400278490000700292520050300299100002600802700002600828700002200854856013000876 2021 eng d a1477-052000aBismuth(iii)-catalyzed bis-cyclization of propargylic diol-esters: a unified approach for the synthesis of [5,5]- and [6,5]-oxaspirolactones0 aBismuthiiicatalyzed biscyclization of propargylic diolesters a u cAUG  a6618-66220 v193 aHerein we disclose an unprecedented intramolecular cascade strategy for the construction of alpha,beta-unsaturated [5,5]- and [6,5]-oxaspirolactones that capitalizes on the pi-electrophilic Lewis acid-catalyzed 5-exo-dig or 6-exo-dig mode of cyclization of propargylic diol esters, followed by dehydration and spirolactonization steps. Moreover, semi-protected substrates also delivered the respective oxaspirolactones with the same ease and in appreciable yields under optimal reaction conditions.1 aKambale, Digambar, A.1 aBorade, Balasaheb, R.1 aKontham, Ravindar uhttps://library.ncl.res.in/content/bismuthiii-catalyzed-bis-cyclization-propargylic-diol-esters-unified-approach-synthesis-5502024nas a2200193 4500008004100000022001400041245003600055210003600091260000800127300001100135490000700146520151300153653001801666653001801684653002401702100001801726700001901744856006701763 2021 eng d a1478-396700aBoolean model for melanogenesis0 aBoolean model for melanogenesis cMAR a0260040 v183 a

Melanogenesis is a highly regulated process through which the pigment melanin is produced in skin cells. Irregularities in the molecular events that govern the process of skin pigmentation can cause disorders like vitiligo. In order to understand the biology of disease progression, it is important to have an in depth understanding of intracellular events. Mathematical models provide an integrated view of intracellular signalling. There are very few models to date that incorporate intracellular processes relevant to melanogenesis and only one to our knowledge that simulates the dynamics of response to varying levels of input. Here, we report the formulation of the largest Boolean model (265 nodes) for melanogenesis to date. The model was built on the basis of a detailed interaction network graph published by Raghunath et al. Through additional manual curation of the reported interactions, we converted the graph into a set of Boolean rules, following the procedure of the first Boolean model (62 nodes) for melanogenesis published by Lee et al. Simulations show that the predicted response to varying UV levels for most of the nodes is similar to the predictions of the existing model. The greater complexity allows investigation of the sensitivity of melanin to additional nodes. We carried out perturbation analysis of the network through node deletion and constitutive activation to identify sensitivity of outcomes, and compared the nodes identified as sensitive to previous reports.

10aBoolean model10amelanogenesis10aSignal transduction1 aDnyane, Pooja1 aGadgil, Chetan uhttps://library.ncl.res.in/content/boolean-model-melanogenesis02151nas a2200241 4500008004100000245011400041210006900155260000800224300001200232490000600244520132800250100001701578700002101595700001701616700002001633700001901653700002501672700001701697700002201714700002001736700002101756856013201777 2022 eng d00aBehavioral and proteomic studies reveal methylglyoxal activate pathways associated with alzheimer’s disease0 aBehavioral and proteomic studies reveal methylglyoxal activate p cDEC a65–750 v63 a

Diabetes is one of the major risk factors for Alzheimer’s disease (AD) development. The role of elevated levels of glucose, methylglyoxal (MGO), and advanced glycation end products (AGEs) in the pathogenesis of AD is not well understood. In this pursuit, we studied the role of methylglyoxal in the pathogenesis of AD in rat models. The elevated plus-maze (EPM) behavioral study indicated that MGO induces anxiety. Treatment of telmisartan (RAGE expression inhibitor) and aminoguanidine (MGO quencher) attenuated MGO induced anxiety. Further, hippocampal proteomics demonstrated that MGO treated rats differentially regulate proteins involved in calcium homeostasis, mitochondrial functioning, and apoptosis, which may affect neurotransmission and neuronal plasticity. The hippocampal tau phosphorylation level was increased in MGO treated rats, which was reduced in the presence of aminoguanidine and telmisartan. The plasma fructosamine level was increased upon MGO treatment. Hippocampal histochemistry showed vascular degeneration and neuronal loss upon MGO treatment. This study provides mechanistic insight into the role of MGO in the diabetes-associated development of AD.

1 aPatil, Gouri1 aKulsange, Shabda1 aKazi, Rubina1 aChirmade, Tejas1 aKale, Vaikhari1 aMote, Chandrashekhar1 aAswar, Manoj1 aKoratkar, Santosh1 aAgawane, Sachin1 aKulkarni, Mahesh uhttps://library.ncl.res.in/content/behavioral-and-proteomic-studies-reveal-methylglyoxal-activate-pathways-associated-alzheimer02385nas a2200349 4500008004100000245014400041210006900185260000800254300001100262490000600273520123500279653001301514653002401527653001401551653003401565653001301599653001401612653002201626653004301648653001801691100001601709700002301725700001801748700003301766700001701799700001601816700002401832700001701856700001601873700001801889856012801907 2022 eng d00aBESFA: bioinformatics based evolutionary, structural & functional analysis of prostate, Placenta, Ovary, Testis, and Embryo (POTE) paralogs0 aBESFA bioinformatics based evolutionary structural functional an cSEP ae104760 v83 a

The POTE family comprises 14 paralogues and is primarily expressed in Prostate, Placenta, Ovary, Testis, Embryo (POTE), and cancerous cells. The prospective function of the POTE protein family under physiological conditions is less understood. We systematically analyzed their cellular localization and molecular docking analysis to elucidate POTE proteins' structure, function, and Adaptive Divergence. Our results suggest that group three POTE paralogs (POTEE, POTEF, POTEI, POTEJ, and POTEKP (a pseudogene)) exhibits significant variation among other members could be because of their Adaptive Divergence. Furthermore, our molecular docking studies on POTE protein revealed the highest binding affinity with NCI-approved anticancer compounds. Additionally, POTEE, POTEF, POTEI, and POTEJ were subject to an explicit molecular dynamic simulation for 50ns. MM-GBSA and other essential electrostatics were calculated that showcased that only POTEE and POTEF have absolute binding affinities with minimum energy exploitation. Thus, this study's outcomes are expected to drive cancer research to successful utilization of POTE genes family as a new biomarker, which could pave the way for the discovery of new therapies.

10a(MMGBSA)10aAdaptive divergence10aEvolution10ageneralized born surface area10aHomology10aMechanics10aMolecular docking10aMolecular dynamic simulation molecular10aPOTE paralogs1 aQazi, Sahar1 aJit, Bimal, Prasad1 aDas, Abhishek1 aKarthikeyan, Muthukumarasamy1 aSaxena, Amit1 aRay, M., D.1 aSingh, Angel, Rajan1 aRaza, Khalid1 aJayaram, B.1 aSharma, Ashok uhttps://library.ncl.res.in/content/besfa-bioinformatics-based-evolutionary-structural-functional-analysis-prostate-placenta01589nas a2200205 4500008004100000022001400041245010200055210006900157260000800226490000800234520086200242653001601104653002801120653002901148653001401177653002701191100001601218700001701234856013201251 2022 eng d a1022-135200aBiodegradable, solvent-free photocrosslinkable PLLA resin formulations for additive manufacturing0 aBiodegradable solventfree photocrosslinkable PLLA resin formulat cNOV0 v2233 a

Acrylate end-functionalized linear and star poly l-lactide (PLLA) based aliphatic, photocurable oligomeric crosslinkers are developed, which are formulated with hydroxyl (ethyl)methacrylate (HEMA) as a biodegradable reactive diluent to demonstrate successful application for solvent-less 3D printing using digital light processing (DLP) technique as well as a custom-designed direct ink writing 3D printer. The resin formulation for 3D printing is optimized using spot curing, photodifferential scanning calorimetry and cure depth analysis. The 3D printed parts exhibit promising mechanical property along with improved hydrophilicity and biodegradability. The development of new photocurable resin formulations are expected to meet the growing demand for raw materials that can be applied for developing custom designed bio implantable objects.

10a3D printing10abiodegradable materials10adigital light processing10aL-Lactide10aphotocurable materials1 aPal, Shibam1 aAsha, S., K. uhttps://library.ncl.res.in/content/biodegradable-solvent-free-photocrosslinkable-plla-resin-formulations-additive-manufacturing02661nas a2200241 4500008004100000022001400041245013400055210006900189260000800258300001100266490000700277520181500284653001902099653001302118653001502131653002302146653001802169100002902187700002102216700001902237700003402256856012902290 2022 eng d a1773-224700aBioinspired hyaluronic acid based nanofibers immobilized with 3, 4-difluorobenzylidene curcumin for treating bacterial infections0 aBioinspired hyaluronic acid based nanofibers immobilized with 3  cAUG a1034800 v743 a

Curcumin (Cur) is a natural polyphenol with multifaceted pharmacological functions, exploited extensively for biomedical applications. Traditionally curcumin is being used as an antimicrobial agent. However, to improvise the pharmacological properties, it is being modified synthetically. One of such modified Cur is 3, 4- difluorobenzylidene curcumin (CDF) which is aimed for enhancing the anti-cancer properties. Though there are reports on the studies of anti-cancer properties involving CDF, the anti-bacterial property is yet to be demonstrated. Accordingly, in our studies, we prepared bioinspired hyaluronic acid blends immobilized with CDF and fabricated non-woven nanofiber mats. These nanofiber mats were characterized and demonstrated in vitro cell culture studies, which involved cell viability, hemolysis, anti-bacterial and cell scratch assay to understand their efficacy in treating bacteria. The molecular docking studies of CDF and Cur were performed on the dihydrofolate reductase (DHFR) enzyme receptor, which is an essential protein of S.auerus (Staphylococcus aureus). The results of MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay, and hemolysis of the respective nanofiber mats with Cur and CDF showed non-toxicity and were compatible with blood cells. Further, the cell proliferation and adherence recorded >60% fibroblast cells for the nanofiber mats. The anti-bacterial property of Cur and CDF was similar. The in vitro release studies for the respective Cur and CDF loaded nanofiber mats recorded a release of 25 and 37%, respectively. From these studies, we concluded that the CDF sustained its antibacterial property in addition to the improved anti-cancer property; hence CDF being synergetic, it will have a better scope in cancer therapy.

10aanti-bacterial10acurcumin10ananofibers10atissue engineering10awound Healing1 aPatel, Pratikshkumar, R.1 aSingam, Amarnath1 aIyer, Arun, K.1 aGundloori, Rathna, Venkata Na uhttps://library.ncl.res.in/content/bioinspired-hyaluronic-acid-based-nanofibers-immobilized-3-4-difluorobenzylidene-curcumin02127nas a2200265 4500008004100000022001400041245010800055210006900163260000800232300001400240490000700254520123000261653001501491653001701506653001601523653003801539100002401577700002101601700002101622700002201643700002701665700002601692700001801718856012501736 2022 eng d a0170-083900aBiological macromolecule chitosan grafted co-polymeric composite: bio-adsorption probe on cationic dyes0 aBiological macromolecule chitosan grafted copolymeric composite  cNOV a9441-94550 v793 a

Chitosan biological macromolecule is a versatile polymer; chemical modification has been carried out that lead to the formation of chitosan grafted polymers composites (Chito-g-PC). We proposed synthesis of six various Chito-g-PC as sorbents for toxic dyes. A novel graft copolymerization method based on radical polymerization with vinyl monomer like acrylic acid, acrylamide, N-isopropylacrylamide, methacrylic acid and polyacrylonitrile were utilized in order to address the large amount of swelling at four different pH buffers solution. The effect of initiator and monomer concentration, time and temperature on % grafting and % grafting efficiency were performed. Comparative characterization of Chito and Chito-g-PC were evaluated by SEM, XRD and FTIR, as well as solubility characteristics of the composites were determined by various pH buffer solution. Cationic toxic dyes Malachite green (MG) and Methylene blue (MB) were selected as the sorbet, and Chito-g-PC were used as biosorbents. Thermodynamic analysis showed that the sorption process was spontaneous and endothermic with an increased randomness. The sorption experiments were realized with six different Chito-g-PC for MG and MB at various pH.

10aAcrylamide10aacrylic acid10aBio sorbent10aChitosan biological macromolecule1 aKhairkar, Shyam, R.1 aPansare, Shubham1 aShedge, Amol, A.1 aChhatre, Shraddha1 aKulal, Dnyaneshwar, K.1 aPatil, Vishwanath, R.1 aPansare, Amol uhttps://library.ncl.res.in/content/biological-macromolecule-chitosan-grafted-co-polymeric-composite-bio-adsorption-probe01994nas a2200217 4500008004100000022001400041245009600055210006900151260000800220300001200228490000700240520121800247653002701465653003901492653002401531653001501555653002801570100002101598700003001619856012701649 2022 eng d a0141-549200aBiosynthetic process and strain improvement approaches for industrial penicillin production0 aBiosynthetic process and strain improvement approaches for indus cFEB a179-1920 v443 a

Penicillins and cephalosporins are the most important class of beta (beta) lactam antibiotics, accounting for 65% total antibiotic market. Penicillins are produced by Penicillium rubens (popularly known as P. chrysogenum) were used to synthesize the active pharmaceutical intermediate (API), 6-aminopenicillinic acid (6-APA) employed in semisynthetic antibiotic production. The wild strains produce a negligible amount of penicillin (Pen). High antibiotic titre-producing P. chrysogenum strains are necessitating for industrial Pen production to meet global demand at lower prices. Classical strain improvement (CSI) approaches such as random mutagenesis, medium engineering, and fermentation are the cornerstones for high-titer Pen production. Since, Sir Alexander Fleming Discovery of Pen, great efforts are expanded to develop at a commercial scale antibiotics producing strains. Breakthroughs in genetic engineering, heterologous expression and CRISPR/Cas9 genome editing tools opened a new window for Pen production at a commercial scale to assure health crisis. The current state of knowledge, limitations of CSI and genetic engineering approaches to Pen production are discussed in this review.

10abeta-lactam antibiotic10aClassical strain improvement (CSI)10aGenetic engineering10aPenicillin10aPenicillium chrysogenum1 aSawant, Amol, M.1 aVamkudoth, Koteswara, Rao uhttps://library.ncl.res.in/content/biosynthetic-process-and-strain-improvement-approaches-industrial-penicillin-production02461nas a2200253 4500008004100000022001400041245009800055210006900153260000800222300001100230490000700241520162000248653001901868653001701887653002001904653002701924653001501951653001701966100002901983700002102012700001702033700003402050856012302084 2022 eng d a1773-224700aBlend of neem oil based polyesteramide as magnetic nanofiber mat for efficient cancer therapy0 aBlend of neem oil based polyesteramide as magnetic nanofiber mat cSEP a1036290 v753 a

Stearic acid-coated magnetic nanoparticles (SMN) and FU (5-Fluorouracil) were immobilized in the blends of neem oil-based polyesteramide and fabricated as nanofiber mat (NM) for controlled release of FU under the influence of an external magnetic field for targeted drug delivery to treat cancer efficiently. Analyzed the surface morphology of the fibers using E-SEM, it was observed that the fibers were smooth with the diameter ranging from 250 to 450 nm. TEM studies showed the uniform distribution of SMN in the nanofibers. The physico-chemical properties of NM and raw materials were analyzed using FTIR, TGA, and XRD. The results suggested that the polymers were well blended. In-vitro FU release studies of the NMs recorded a significant difference in the cumulative percentage of FU release from SMN-NMs. The SMN-NMs released 95% of FU in 4 h, whereas, NMs released 83% of FU in 24 h. The cell viability assay for the NM was evaluated in the L929 mouse fibroblast cells, where >75% of cells were viable. The hemolysis assay for the developed SMN-NF showed <5% of hemolysis, which indicated the NMs were safe for application. The anti-cancer activity of FU loaded SMN-NF was analyzed in the MCF-7 cancer cell line, which recorded more than 50% cell death within 24 h. From SQUID analysis, we found that the 10% SMN were superparamagnetic in nature, the magnetization at 30 kOe was observed to be 4.3 emu/g. Based on the in vitro results, we concluded that the developed SMN-NMs are recommended for in vivo studies to understand their efficacy for the targeted drug delivery to treat cancer.

10a5-Fluorouracil10adrug release10aelectrospinning10aMagnetic nanoparticles10ananofibers10aStearic acid1 aPatel, Pratikshkumar, R.1 aSingam, Amarnath1 aDadwal, Arun1 aGundloori, Rathna, Venkata Na uhttps://library.ncl.res.in/content/blend-neem-oil-based-polyesteramide-magnetic-nanofiber-mat-efficient-cancer-therapy01915nas a2200193 4500008004100000022001400041245010900055210006900164260000800233300001600241490000800257520123200265100001801497700002301515700002201538700001801560700001801578856012501596 2022 eng d a1520-610600aBlue fluorescence of cyano-tryptophan predicts local electrostatics and hydrogen bonding in biomolecules0 aBlue fluorescence of cyanotryptophan predicts local electrostati cDEC a10732-107400 v1263 a

Cyano-tryptophan is an unnatural fluorescent amino acid that emits in the visible region. Along with the structural similarity with tryptophan, the unique photophysical properties of this fluorophore make it an ideal probe for biophysical research. Herein, combining fluorescence spectroscopy, infrared spectroscopy, and molecular dynamics simulations, we show that the cyano-tryptophan's emission energy quantifies the underlying bond-specific noncovalent interactions in terms of the electric field. We further report the use of fluorophore's emission energy to predict its hydrogen bond characteristics. We demonstrate that combining experiments with molecular dynamics simulations can provide the hydrogen bonding status of the nitrile moiety. In addition, we report a method to differentiate between aqueous and nonaqueous hydrogen-bonding partners. Using a phenomenological approach, we demonstrate that the presence of the cyano-indole moiety is responsible for the distinct correlations between the fluorophore's emission and the electrostatic forces on the nitrile bond. As indole is a privileged scaffold for both native amino acids and nucleobases, cyano-indoles will have many multifaceted applications.

1 aHaldar, Tapas1 aChatterjee, Srijan1 aAlam, Md, Nirshad1 aMaity, Pradip1 aBagchi, Sayan uhttps://library.ncl.res.in/content/blue-fluorescence-cyano-tryptophan-predicts-local-electrostatics-and-hydrogen-bonding02213nas a2200313 4500008004100000022001400041245014500055210006900200260000800269300001600277490000700293520116000300653001701460653003401477653001901511653002801530653002301558653001901581100002801600700001301628700001701641700001901658700001901677700002501696700002001721700001801741700001401759856012601773 2022 eng d a2155-543500aBoosting electrochemical CO2 reduction to methane via tuning oxygen vacancy concentration and surface termination on a copper/ceria catalyst0 aBoosting electrochemical CO2 reduction to methane via tuning oxy cSEP a10973-109830 v123 a

Metal oxides are a promising material for designing highly active and selective catalysts for the electrochemical reduction of carbon dioxide (CO2RR). Here, we designed a Cu/ceria catalyst with high selectivity of methane production at single-atomic Cu active sites. Using this, we report favorable design concepts that push the product selectivity of methane formation by combining detailed structural analysis, density functional theory (DFT), in situ Raman spectroscopy, and electrochemical measurements. We demonstrate that a higher concentration of oxygen vacancies on the catalyst surface, resulting from more available Cu+ sites, enables high selectivity for methane formation during CO2RR and can be controlled by the calcination temperature. The DFT calculation and in situ Raman studies indicate that pH controls the surface termination; a more alkaline pH generates hydroxylated surface motifs with more active sites for the hydrogen evolution reaction. These findings provide insights into designing an efficient metal oxide electrocatalyst by controlling the atomic structure via the reaction environment and synthesis conditions.

10acopper-ceria10aelectrochemical CO2 reduction10aelectrolyte pH10agas diffusion electrode10amethane production10aOxygen vacancy1 aPatra, Kshirodra, Kumar1 aLiu, Zhu1 aLee, Hojeong1 aHong, Seungwon1 aSong, Hakhyeon1 aAbbas, Hafiz, Ghulam1 aKwon, Youngkook1 aRinge, Stefan1 aOh, Jihun uhttps://library.ncl.res.in/content/boosting-electrochemical-co2-reduction-methane-tuning-oxygen-vacancy-concentration-and01704nas a2200205 4500008004100000022001400041245009100055210006900146260000800215300001600223490000700239520100400246100002301250700002001273700002701293700002101320700002001341700002101361856011601382 2022 eng d a2041-652000aBQ-AurIPr: a redox-active anticancer Au(i) complex that induces immunogenic cell death0 aBQAurIPr a redoxactive anticancer Aui complex that induces immun cSEP a10779-107850 v133 a

Immunogenic Cell Death (ICD) is a unique cell death mechanism that kills cancer cells while rejuvenating the anticancer immunosurveillance, thereby benefiting the clinical outcomes of various immuno-chemotherapeutic regimens. Herein, we report development of a library of benzo[a]quinolizinium-based Au(i) complexes through an intramolecular amino-auration reaction of pyridino-alkynes. We tested 40 candidates and successfully identified BQ-AurIPr as a novel redox-active Au(i) complex with potent anticancer properties. BQ-AurIPr efficiently triggered generation of DAMPs - the hallmarks of ICD - and was superior in terms of efficiency compared to FDA-approved drugs known to induce ICD. BQ-AurIPr significantly increased immunogenicity of cancer cells enhancing their phagocytosis when co-cultured with immune cells. Our investigation reveals that BQ-AurIPr induces oxidative stress inside mitochondria leading to mitophagy, as the mechanism for immunogenic cell death in A549 cells.

1 aMule, Ravindra, D.1 aKumar, Akhilesh1 aSancheti, Shashank, P.1 aSenthilkumar, B.1 aKumar, Himanshu1 aPatil, Nitin, T. uhttps://library.ncl.res.in/content/bq-auripr-redox-active-anticancer-aui-complex-induces-immunogenic-cell-death02917nas a2200229 4500008004100000022001400041245015400055210006900209260000900278300001600287490000700303520206700310100002702377700002102404700002302425700002102448700002302469700002002492700001802512700003002530856012702560 2023 eng d a2050-748800aBaby step in assembling and integrating the components of an artificial photosynthesis device with forced heterojunctions towards improved efficiency0 aBaby step in assembling and integrating the components of an art cJUL  a15168-151820 v113 a

How to achieve unassisted, economical, scalable, and sustainable artificial photosynthesis for liquid fuels/products with improved solar-to-fuel efficiency (STFE) to address a carbon-neutral economy remains a big question. To a large degree, the extent of charge separation at heterojunction interfaces and charge utilization determine the STFE. Towards this, BiVO3 is assembled from ionic-precursors into TiO2 pores, and integrated structurally and electronically with TiO2 on calcination as BiVO4 quantum dots (BVQDs). BVQDs in TiO2 (BVT) pores lead to an all-inorganic system with a sub-quadrillion number of heterojunctions in a 1 cm(2) device (contains similar to 25 mu g of BiVO4 (similar to 2.5 wt%) in the nanopores of similar to 975 mu g of TiO2 (similar to 97.5 wt%)) and facilitate artificial photosynthesis. We demonstrate 31-38% STFE with a photon to chemical conversion turn over frequency (ToF(P2C)) of 2.73 s(-1) with a 1 cm(2) wireless BiVO4-TiO2 artificial leaf (BVT-AL) device for HCHO and CH3OH. The sequential nature of CO2 reduction to HCHO and then to CH3OH is evident from the reaction results. (CO2)-C-13 isotopic labeling experiments confirm that the input CO2 is the source for product formation. A large increase in the photocurrent density and incident photon-to-current efficiency (IPCE) of BVT, over 100% for the BiVO4 photoanode in visible light, demonstrates and supports efficient visible light absorption, charge separation and migration to the redox sites. A device has been demonstrated to show sustainable activity in direct sunlight, and addresses scalability from 1 to 9 cm(2). Assuming no change (50% decrease) in the STFE, a 6.74 m(2) device is expected to convert 1 (0.5) kg h(-1) CO2 into C1-oxygenates in sunlight. DFT calculations carried out with anatase TiO2 (101) and BiVO4 (121) interfaces support many of the experimental findings, including electron flow from the latter to the former, and interaction of the oxygen of TiO2 with BiVO4 and vice versa at the interface towards forced heterojunctions.

1 aSalgaonkar, Kranti, N.1 aBajpai, Himanshu1 aMhamane, Nitin, B.1 aNalajala, Naresh1 aChauhan, Inderjeet1 aThakkar, Kavita1 aJoshi, Kavita1 aGopinath, Chinnakonda, S. uhttps://library.ncl.res.in/content/baby-step-assembling-and-integrating-components-artificial-photosynthesis-device-forced02240nas a2200277 4500008004100000022001400041245007900055210006900134260000800203300001100211490000800222520137500230653002001605653001501625653000901640653002201649653001101671653002001682653002401702100001801726700002101744700002301765700002701788700003601815856011101851 2023 eng d a0141-813000aBacopa monnieri reduces Tau aggregation and Tau-mediated toxicity in cells0 aBacopa monnieri reduces Tau aggregation and Taumediated toxicity cAPR a1231710 v2343 a

Alzheimer's disease is a neurodegenerative disease characterized by progressive memory loss and behavioral impairments. In the present study, the ethanolic extract of Bacopa monnieri was studied for its potency to inhibit Tau aggregation and rescuing of the viability of Tau-stressed cells. Bacopa monnieri was observed to inhibit the Tau aggregation in vitro. The cells exposed to Bacopa monnieri were also observed to have a low level of ROS and caspase-3 activity. The immunoblot and immunofluorescence analysis showed that Bacopa monnieri acts as an antioxidant and restored the Nrf2 levels in Neuro2a cells. Bacopa monnieri treatment to Neuro2a cells was observed to reduce the phospho-Tau load in formaldehyde-stressed cells. Furthermore, the treatment of Bacopa monnieri reduced the phosphorylation of GSK-3 beta in formaldehyde-stressed cells. Ran and NUP358 are the key proteins involved in nuclear transport. It was observed that formaldehyde treatment impaired the nuclear transport by missorting the NUP358 arrangement in Neuro2a cells. On the contrary, Bacopa monnieri treatment restored the NUP358 arrangement in cells. The overall results of the present study suggested that Bacopa monnieri could be considered a potent herb against Tau phosphorylation and Tau aggregation, which projects it as a promising formulation for Alzheimer's disease.

10aBacopa monnieri10aGSK-3 beta10aNrf210aNuclear transport10aNUP35810aTau Aggregation10aTau phosphorylation1 aDubey, Tushar1 aKushwaha, Preeti1 aThulasiram, H., V.1 aChandrashekar, Madhura1 aChinnathambi, Subashchandrabose uhttps://library.ncl.res.in/content/bacopa-monnieri-reduces-tau-aggregation-and-tau-mediated-toxicity-cells02549nas a2200241 4500008004100000022001400041245009700055210006900152260000800221300000800229490000700237520175400244100001501998700002102013700001802034700002302052700001902075700002502094700001902119700002502138700002302163856012102186 2023 eng d a0343-865100aBacterial communities and diversity of western ghats soil: a study of a biodiversity hotspot0 aBacterial communities and diversity of western ghats soil a stud cAPR a1080 v803 a

The Western Ghats is one of India's mega-diversity hotspots and an ecologically and geologically important area for the diversity of endemic plants and animals. The present study provides insights into the aerobic bacterial diversity and composition of the soils of North Western Ghats located in Maharashtra state (NWGM), India. The samples for the culture-dependent study were collected from 6 different locations namely Malshej Ghat, Bhimashankar, Lonavala, Mulshi, Tail-Baila, and Mahabaleshwar. A total of 173 isolates were obtained from the different samples, which belonged to Proteobacteria (43%), Firmicutes (36%), and Actinobacteria (19%). Sequences of 15 strains shared <= 98.7% similarity (a species cut-off) which represent potential novel species. Metagenomic analysis revealed the presence of Actinobacteria and Proteobacteria as the most dominant phyla at both MB and MG. However, both sites showed variation in the composition of rare phyla and other dominant phyla. This difference in bacterial community composition could be due to differences in altitude or other physicochemical properties. The functional prediction from the amplicon sequencing showed the abundance of carbohydrate, protein, and lipid metabolism which was corroborated by screening the isolated bacterial strains for the same. The present study has a unique take on microbial diversity and defines the importance of community assembly processes such as drift, dispersal, and selection. Such processes are relatively important in controlling community diversity, distribution, as well as succession. This study has shown that the microbial community of NWGM is a rich source of polysaccharide degrading bacteria having biotechnological potential.

1 aGhare, Uma1 aNarvekar, Simran1 aLodha, Tushar1 aMallebhari, Rubiya1 aDastager, Syed1 aBarvkar, Vitthal, T.1 aDhotre, Dhiraj1 aKarmalkar, Nitin, R.1 aPable, Anupama, A. uhttps://library.ncl.res.in/content/bacterial-communities-and-diversity-western-ghats-soil-study-biodiversity-hotspot02358nas a2200253 4500008004100000245011500041210006900156260000800225300000900233490000700242520149500249100002701744700002301771700001801794700002001812700002001832700002601852700001601878700002001894700002401914700002001938700001901958856012701977 2023 eng d00aBAHD-type acyltransferase concludes the biosynthetic pathway of non-bitter glycoalkaloids in ripe tomato fruit0 aBAHDtype acyltransferase concludes the biosynthetic pathway of n cJUL a45400 v143 a

During tomato fruit ripening, bitter and toxic steroidal glycoalkaloids (SGAs) are converted to nonbitter and less toxic forms, but proposed acylating enzyme in pathway remain unknown. Here, authors report BAHD-type acyltransferase that catalyze acylation step in biosynthesis of non-bitter SGAs in tomato. Tomato is the highest value fruit and vegetable crop worldwide, yet produces & alpha;-tomatine, a renowned toxic and bitter-tasting anti-nutritional steroidal glycoalkaloid (SGA) involved in plant defense. A suite of modifications during tomato fruit maturation and ripening converts & alpha;-tomatine to the non-bitter and less toxic Esculeoside A. This important metabolic shift prevents bitterness and toxicity in ripe tomato fruit. While the enzymes catalyzing glycosylation and hydroxylation reactions in the Esculeoside A pathway have been resolved, the proposed acetylating step remains, to date, elusive. Here, we discovered that GAME36 (GLYCOALKALOID METABOLISM36), a BAHD-type acyltransferase catalyzes SGA-acetylation in cultivated and wild tomatoes. This finding completes the elucidation of the core Esculeoside A biosynthetic pathway in ripe tomato, allowing reconstitution of Esculeoside A production in heterologous microbial and plant hosts. The involvement of GAME36 in bitter SGA detoxification pathway points to a key role in the evolution of sweet-tasting tomato as well as in the domestication and breeding of modern cultivated tomato fruit.

1 aSonawane, Prashant, D.1 aGharat, Sachin, A.1 aJozwiak, Adam1 aBarbole, Ranjit1 aHeinicke, Sarah1 aAlmekias-Siegl, Efrat1 aMeir, Sagit1 aRogachev, Ilana1 aO'Connor, Sarah, E.1 aGiri, Ashok, P.1 aAharoni, Asaph uhttps://library.ncl.res.in/content/bahd-type-acyltransferase-concludes-biosynthetic-pathway-non-bitter-glycoalkaloids-ripe01705nas a2200205 4500008004100000022001400041245009200055210006900147260000900216300001000225490000600235520104200241653001001283653001101293653001001304653001201314100002601326700002301352856012401375 2023 eng d a2058-988300aBatch and continuous flow mechanochemical synthesis of organic compounds including APIs0 aBatch and continuous flow mechanochemical synthesis of organic c cDEC  a10-250 v93 a

Mechanochemistry is becoming an enabling technology for the synthesis of organic and inorganic compounds as well as for the synthesis of polymers as it underlines sustainability in a significant manner. Continuous mechanochemical synthesis further adds value to the approach through consistency, smaller footprint, and better energy efficiency. This review gives an indepth view of the present status of this subject along with critical engineering aspects that one needs to measure and monitor as eventually synthesis needs to be transformed into a process. The examples covered herein include the synthesis of organic compounds, viz., APIs, agrochemical intermediates, catalysts, and polymers. In the end, we also discuss the safety aspects of mechanochemical synthesis and recommendations for exploring this field further. Mechanochemistry is becoming an enabling technology for the synthesis of organic and inorganic compounds as well as for the synthesis of polymers as it underlines sustainability in a significant manner.

10aacids10aAmides10aForce10asolvent1 aAtapalkar, Ranjit, S.1 aKulkarni, Amol, A. uhttps://library.ncl.res.in/content/batch-and-continuous-flow-mechanochemical-synthesis-organic-compounds-including-apis03357nas a2200265 4500008004100000022001400041245011900055210006900174260000800243300001100251490000800262520246800270653001002738653001302748653002602761653002202787653002502809100002002834700002302854700002002877700002602897700002102923700001702944856013002961 2023 eng d a0167-273800aBenign strategy toward mesoporous carbon coated Sb nanoparticles: a high-performance Li-ion/Na-ion batteries anode0 aBenign strategy toward mesoporous carbon coated Sb nanoparticles cAUG a1162430 v3963 a

Antimony (Sb)-based anodes can offer excellent gravimetric capacity (-660 mAhg(1)) in lithium-ion/sodium-ion batteries (LIBs/SIBs) fabricated using carbonate-based electrolytes complexed with lithium/sodium salt. However, high first-cycle irreversible loss (35-40%) and gradual capacity fade (25-30%/cycle) originate from solid electrolyte interphase (SEI), and severe volumetric stress (-300%) associated with alloyed phase(s) impede reallife applications. Herein, we devise a benign strategy to develop mesoporous carbon coating onto antimony nanoparticles (Sb@C) based core-shell architecture for LIBs/SIBs anode. In particular, -30-50 nm thick mesoporous carbon spheres (-1 +/- 0.5 mu m) were obtained from resorcinol-formaldehyde (RF)-based polycondensation reaction by sol-gel chemistry engulfing Sb nanoparticles by suitably controlling Cetyltrimethylammonium bromide (CTAB)-induced steric stabilization and pH modulation during synthesis. The core-shell Sb@C helps faster Li+/Na+-ion migration preventing the structural collapse of Sb during electrochemical cycling and thereby improving the capacity fade. Electrochemical results demonstrate Sb@C can deliver a specific capacity of -536 mAhg(-1) and - 291 mAhg(-1) at 0.1C current rate in LIBs and SIBs, respectively, up to 200 cycles. Electrochemical impedance spectroscopy (EIS) indicates lower charge transfer (Rct) and SEI resistance (RSEI) of Sb@C cycled electrode than the bare Sb-NPs was the probable reason for improved Li/Na-ion storage in Sb@C anode. A detailed galvanostatic intermittent titration technique (GITT) and internal resistance measurements during 1st and 2nd cycles shed light on distinguishably different Li-ion/Na-ion storage behavior. The bulk Li+/Na+-ion diffusion coefficients found diminishes at reaction voltages (0.9 V/0.6 V for lithiation and 0.6 V/0.4 V for sodiation) corresponding with alloyed phase(s) concurrent with a drop in internal resistance at the quasi-opencircuit voltage (QOCV) during 1st and 2nd discharge cycle. On the contrary, de-alloying phenomena from the fully lithiated/sodiated phase(s) display an entirely opposite trend. The Li+ diffusion coefficient reaches minima at -1.1 V with a sudden jump in the internal resistance at QOCV during 1st and 2nd charge cycle. However, Na+ diffusion coefficient gradually drops along with a steep increase in the internal resistance, indicating partial Naion trapping and irreversible capacity loss.

10aanode10aAntimony10alithium-ion batteries10amesoporous carbon10aSodium-ion batteries1 aDashairya, Love1 aChaturvedi, Vikash1 aKumar, Abhishek1 aMohanta, Tandra, Rani1 aShelke, Manjusha1 aSaha, Partha uhttps://library.ncl.res.in/content/benign-strategy-toward-mesoporous-carbon-coated-sb-nanoparticles-high-performance-li-ionna01162nas a2200157 4500008004100000022001400041245010200055210006900157260000800226490000700234520056300241100002200804700002500826700002200851856013100873 2023 eng d a1861-472800aBF3.Et2O-catalyzed selective C-4 alkylation of isoquinolin1(2H)-ones employing p-quinone methides0 aBF3Et2Ocatalyzed selective C4 alkylation of isoquinolin12Hones e cSep0 v183 a

The direct C-4 alkylation of isoquinolin-1(2H)-one moiety is a challenging transformation in organic synthesis. Here we present a practical and efficient synthesis of C-4 alkylated isoquinolin-1(2H)-ones through conjugate addition of isoquinolin-1(2H)-ones to p-quinone methides for the first time. The process is facilitated by Lewis acid catalysis and this operationally straightforward, mild, metal-free and one-pot transformation provides a wide range of C-4 alkylated isoquinolin-1(2H)ones at ambient temperature in good to excellent yields.

1 aMore, Devidas, A.1 aGhotekar, Ganesh, S.1 aMuthukrishnan, M. uhttps://library.ncl.res.in/content/bf3et2o-catalyzed-selective-c-4-alkylation-isoquinolin12h-ones-employing-p-quinone-methides01512nas a2200169 4500008004100000022001400041245015600055210006900211260000800280300001400288490000700302520082800309100002201137700003201159700002201191856012901213 2023 eng d a0022-326300aBi(III)-catalyzed synthesis of substituted furans from hydroxy-oxetanyl ketones: application to unified total synthesis of shikonofurans J, D, E, and C0 aBiIIIcatalyzed synthesis of substituted furans from hydroxyoxeta cMAY a7328-73460 v883 a

We report an improved synthetic protocol for hydroxymethyl-derivedpolysubstituted furans employing Bi-(III)-catalyzed dehydrative cycloisomerizationof alpha-hydroxy oxetanyl ketones. This procedure provides rapidaccess (within 5 min) to highly substituted furans with exceptionalfunctional group diversity, excellent yields, generality, scalability,and operationally simple reaction conditions. Further, it demonstratedthe utility of this method in the first enantioselective total synthesisof furyl-hydroquinone-derived biologically potent natural productsshikonofurans J, D, E, and C in seven linear steps, starting fromreadily available building blocks of 2,5-dihydroxy acetophenone and3-oxetanone employing chiral-phosphoric acid (TRIP)-catalyzed asymmetricprenylation as a key step to induce the chirality.

1 aKataria, Priyanka1 aSahoo, Shubhranshu, Shekhar1 aKontham, Ravindar uhttps://library.ncl.res.in/content/biiii-catalyzed-synthesis-substituted-furans-hydroxy-oxetanyl-ketones-application-unified01451nas a2200289 4500008004100000022001400041245011400055210006900169260000800238300001200246490000700258520049400265653001800759653002900777653001000806653001800816653001000834653002000844100002200864700002400886700002400910700002400934700002900958700002500987700001701012856013201029 2023 eng d a0019-510300aBimetallic quantum dots (Cu-Pd, Ni-Pd) catalyzed reaction of bromo arenes with alkenes and aryl boronic acids0 aBimetallic quantum dots CuPd NiPd catalyzed reaction of bromo ar cAUG a843-8480 v623 a

Bimetallic quantum dots (Cu-Pd and Ni-Pd) are active ligand-free catalysts for the alkenylation and arylation of aryl bromides. The catalysts are easy to synthesize by benign green protocols and characterized by various techniques. The bimetallic QD catalysts synthesized thus are superior to Pd(OAc)2 or other Pd NP catalysis of alkenylation reactions of aryl bromides with similar E-selectivity. The reaction of aryl bromide with aryl boronic acid is facile with high yields.

10aAryl bromides10aBimetallic QDs catalysis10aCu-Pd10aMizoroki-Heck10aNi-Pd10aSuzuki coupling1 aRaut, Pallavi, K.1 aMeroliya, Heena, K.1 aDumbre, Swapnil, R.1 aPatil, Vaishali, N.1 aSolanki, Bhanupratap, S.1 aWaghmode, Shobha, A.1 aIyer, Suresh uhttps://library.ncl.res.in/content/bimetallic-quantum-dots-cu-pd-ni-pd-catalyzed-reaction-bromo-arenes-alkenes-and-aryl-boronic02282nas a2200253 4500008004100000022001400041245009700055210006900152260000800221490000600229520140600235653002501641653002301666653001801689653002901707653003301736100001901769700001701788700004001805700001801845700001701863700002401880856012401904 2023 eng d a2366-748600aBinder-free and flexible VOPO4/CNT film as cathode for aqueous rechargeable Zn-metal battery0 aBinderfree and flexible VOPO4CNT film as cathode for aqueous rec cSEP0 v73 a

Aqueous rechargeable Zn-metal batteries (AZMBs) are promising energy storage aids due to their inherent safety, low cost, and competent performance, with prospects in stationary and portable applications. In this regard, one of the critical requirements is developing electrodes that can adapt to mechanical deformation without compromising the charge storage performance. The current work demonstrates the development of a binder-free and mechanically flexible composite cathode film (VP/fCNT-F, where `F' stands for the film) based on VOPO4 (VP) and functionalized carbon nanotubes (fCNTs). The VP/fCNT-F film processing involves simple vacuum filtration of the composite obtained from the in-situ reaction of the fCNTs and the VP precursor in an aqueous medium. The functionalization of carbon nanotube (CNT) is important for the homogenous dispersion of VP and fCNT. The VP/fCNT-F electrode is used as a monolithic electrode in AZMB cells in combination with both liquid and quasi-solid-state gel polymer electrolytes. Besides, the utility of the VP/fCNT-F electrode in a flexible battery configuration is also demonstrated. Interestingly, in both the coin-cell and flexible configurations, the VP/fCNT-F electrode delivers a comparable discharge capacity of 90 and 78 mAh g(-1), respectively (at 0.1 A g(-1)), validating the advantage of the binder-free VP/fCNT-F electrode for AZMBs.

10abinder-free cathodes10aflexible batteries10alayered VOPO410apolymer gel electrolytes10aself-standing flexible films1 aDilwale, Swati1 aBabu, Athira1 aPuthiyaveetil, Priyanka, Pandinhare1 aKurian, Maria1 aGhosh, Meena1 aKurungot, Sreekumar uhttps://library.ncl.res.in/content/binder-free-and-flexible-vopo4cnt-film-cathode-aqueous-rechargeable-zn-metal-battery02873nas a2200277 4500008004100000022001400041245013300055210006900188260000800257300001200265490000700277520189600284653003802180653002302218653001702241653002302258653002702281100002102308700001902329700001702348700002502365700001802390700002702408700003002435856013002465 2023 eng d a1139-670900aBiosynthesis of gold nanoparticles by Penicillium rubens and catalytic detoxification of ochratoxin A and organic dye pollutants0 aBiosynthesis of gold nanoparticles by Penicillium rubens and cat cNOV a765-7800 v263 a

The environmental pollution caused by chemical dyes is a growing concern nowadays. Limitations of traditional methods opened the route for nanotechnology; owing to the versatile properties of nanomaterials, gold nanoparticles (AuNPs) became a potential strategy for different applications. In the present study, biosynthesis of gold nanoparticles (BioAuNPs) was carried out by reacting chloroauric acid (HAuCl4) with cell-free filtrate of Penicillium rubens sp. nov. NCIM 1937. The AuNPs were then characterized by UV-visible spectroscopy, HR-TEM, FTIR, and DLS analysis to further examine their efficacious biosynthesis and morphological properties including size, shape, and stability. The biogenic AuNPs are polydisperse in nature, with a mean size of 14.92 +/- 5 nm. These AuNPs exhibited promising antimicrobial activity against Escherichia coli NCIM-2065, Bacillus subtilis NCIM-2010, and Penicillium verrucosum MTCC 4935. In vitro quantitative HPLC results revealed that BioAuNPs significantly inhibited the biosynthesis of ochratoxin A (OTA). Microbial fuel cells (MFCs) are intriguing for power generation and wastewater treatment since they can directly transform chemical energy stored in organic matter to electricity by extracellular electron transfer (EET) via membrane proteins. AuNPs also showed excellent potential for dye degradation of organic pollutants, viz., methylene blue (MB), phenol red (PR), bromothymol blue (BTB), Congo red (CR), and 4-nitrophenol (4-NP). All dye removal efficiencies were estimated and fitted to pseudo-first-order processes using kinetic rate constants (Ka).The present study reveals a simple, original, and eco-friendly method for the synthesis of multifunctional biogenic AuNPs that could be effective in OTA detoxification in food products and organic pollutant removal during wastewater treatment for a sustainable environment.

10aCatalytic organic dye degradation10agold nanoparticles10aOchratoxin A10aPenicillium rubens10aPenicillium verrucosum1 aBhandari, Yogesh1 aVarma, Sanjana1 aSawant, Amol1 aBeemagani, Sreelatha1 aJaiswal, Neha1 aChaudhari, Bhushan, P.1 aVamkudoth, Koteswara, Rao uhttps://library.ncl.res.in/content/biosynthesis-gold-nanoparticles-penicillium-rubens-and-catalytic-detoxification-ochratoxin01562nas a2200217 4500008004100000022001400041245014000055210006900195260000900264300001100273490000800284520076300292653002101055653002201076653002501098653002801123653002101151100002801172700002201200856012201222 2023 eng d a0040-403900aBismuth(III)-catalyzed cascade condensation of alkynyl alcohols with terminal alkynes: a facile construction of propargyl cyclic ethers0 aBismuthIIIcatalyzed cascade condensation of alkynyl alcohols wit cJAN  a1542740 v1143 a

Herein, we report a Bi(OTf)3-catalyzed cascade condensation between alkynols (particularly pent-4-yn-1ols and hex-5-yn-1-ols) and terminal alkynes (aryl alkynes) to facile access to diverse propargyl cyclic ethers (2 alkynyl tetrahydrofurans and 2 alkynyl tetrahydropyrans). This simple and efficient cascade transformation proceeds through initial intramolecular hydroalkoxylation of alkynols to give corresponding cyclic enol ethers (via exo-dig or endo-dig mode of ring-closure) followed by hydroalkynylation. Costeffective, naturally abundant, green Lewis acid catalysis, ambient reaction conditions, short reaction times, good substrate scope, and high yields are salient features of this strategy.(c) 2022 Elsevier Ltd. All rights reserved.

10aAlkynyl alcohols10aBismuth catalysis10aCascade condensation10aPropargyl cyclic ethers10aTerminal alkynes1 aPratapure, Madhukar, S.1 aKontham, Ravindar uhttps://library.ncl.res.in/content/bismuthiii-catalyzed-cascade-condensation-alkynyl-alcohols-terminal-alkynes-facile02268nas a2200193 4500008004100000022001400041245011900055210006900174260000800243490000800251520156300259653002801822653001901850653001901869653001601888100001901904700002301923856012801946 2024 eng d a0006-352500aBacterial nanocellulose: a versatile biopolymer production using a cost-effective wooden disc based rotary reactor0 aBacterial nanocellulose a versatile biopolymer production using  cJUL0 v1153 a

Bacterial nanocellulose (BNC) has various unique qualities, including high mechanical strength, crystallinity, and high water-holding capacity, which makes it appropriate for a wide range of industrial applications. But its lower yield coupled with its high production cost creates a barrier to its usage. In this study, we have demonstrated the better yield of BNC from an indigenous strain Komagataeibacter rhaeticus MCC-0157 using a rotary disc bioreactor (RDB) having a wooden disc. The RDB was optimized based on the type of disc material, distance between the disc, and rotation speed to get the highest yield of 13.0 g/L dry material using Hestrin-Schramm (H-S) medium. Further, the bioreactor was compared for the BNC production using reported medium, which is used for static condition; the RDB showed up to fivefold increase in comparison with the static condition reported. Komagataeibacter rhaeticus MCC-0157 was previously reported to be one of the highest BNC producing stains, with 8.37 g/L of dry yield in static condition in 15 days incubation. The designed RDB demonstrated 13.0 g/L dry yield of BNC in just 5 days. Other characteristics of BNC remain same as compared with static BNC production, although the difference in the crystallinity index was observed in RDB (84.44%) in comparison with static (89.74%). For the first time, wooden disc was used for rotary bioreactor approach, which demonstrated higher yield of BNC in lesser time and can be further used for sustainable production of BNC at the industrial level. image

10abacterial nanocellulose10arotary reactor10aSustainability10awooden disc1 aJagtap, Ashish1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/bacterial-nanocellulose-versatile-biopolymer-production-using-cost-effective-wooden-disc01816nas a2200205 4500008004100000022001400041245007800055210006900133260000800202490000800210520111600218653003501334653001901369653001501388653003501403100001901438700001901457700002201476856011201498 2024 eng d a0048-969700aBacteriophages: status quo and emerging trends toward one health approach0 aBacteriophages status quo and emerging trends toward one health  cJAN0 v9083 a

The alarming rise in antimicrobial resistance (AMR) among the drug-resistant pathogens has been attributed to the ESKAPEE group (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, Enterobacter sp., and Escherichia coli). Recently, these AMR microbes have become difficult to treat, as they have rendered the existing therapeutics ineffective. Thus, there is an urgent need for effective alternatives to lessen or eliminate the current infections and limit the spread of emerging diseases under the ``One Health'' framework. Bacteriophages (phages) are naturally occurring biological resources with extraordinary potential for biomedical, agriculture/food safety, environmental protection, and energy production. Specific unique properties of phages, such as their bactericidal activity, host specificity, potency, and biocompatibility, make them desirable candidates in therapeutics. The recent biotechnological advancement has broadened the repertoire of phage applications in nanoscience, material science, physical chemistry, and soft

10aAntimicrobial resistance (AMR)10aBacteriophages10aOne-health10aTherapeutics commercialization1 aSamson, Rachel1 aDharne, Mahesh1 aKhairnar, Krishna uhttps://library.ncl.res.in/content/bacteriophages-status-quo-and-emerging-trends-toward-one-health-approach01810nas a2200205 4500008004100000022001400041245012800055210006900183260000800252300001400260490000700274520103600281100002901317700003001346700002301376700002801399700002601427700002101453856013001474 2024 eng d a1477-052000aBase controlled rongalite-mediated reductive aldol/cyclization and dimerization of isatylidene malononitriles/cyanoacetates0 aBase controlled rongalitemediated reductive aldolcyclization and cFEB a1727-17320 v223 a

In this study, we developed a novel methodology involving a base-controlled, rongalite-mediated reductive/aldol reaction, followed by cyclization of isatylidene malononitriles/cyanoacetates, resulting in the synthesis of spiro[2,3-dihydrofuran-3,3 `-oxindole]. Additionally, we have disclosed a rongalite-mediated dimerization process for isatylidene malononitriles, yielding dispiro[cyclopent-3 `-ene]bisoxindole. The utilization of rongalite in this reaction serves a dual purpose, acting both as a reducing agent and a C1 synthon. The developed approach has several advantages like a simple reaction setup, a wide substrate scope, requiring less time, using water as a green solvent, no metal or catalyst is required and products can be easily isolated via filtration with excellent yields under mild reaction conditions. A novel methodology was developed for the synthesis of spiro[2,3-dihydrofuran-3,3 `-oxindole] and dispiro[cyclopent-3 `-ene]bisoxindole under mild reaction conditions using inexpensive reagents.

1 aChaudhari, Suryakant, S.1 aNichinde, Chandrakant, B.1 aPatil, Baliram, R.1 aGirase, Amardipsing, S.1 aKrishna, Gamidi, Rama1 aKinage, Anil, K. uhttps://library.ncl.res.in/content/base-controlled-rongalite-mediated-reductive-aldolcyclization-and-dimerization-isatylidene02162nas a2200313 4500008004100000022001400041245006800055210006700123260000900190300001600199490000700215520118200222653002401404653002301428653003201451653001601483653003301499100001601532700002101548700002201569700002401591700002701615700001901642700003201661700001601693700002101709700001901730856009901749 2024 eng d a1530-698400aBiexciton emission in CsPbBr3 nanocrystals: polar facet matters0 aBiexciton emission in CsPbBr3 nanocrystals polar facet matters cAUG  a10434-104420 v243 a

The metal halide perovskite nanocrystals exhibit a remarkable tolerance to midgap defect states, resulting in high photoluminescence quantum yields. However, the potential of these nanocrystals for applications in display devices is hindered by the suppression of biexcitonic emission due to various Auger recombination processes. By adopting single-particle photoluminescence spectroscopy, herein, we establish that the biexcitonic quantum efficiency increases with the increase in the number of facets on cesium lead bromide perovskite nanocrystals, progressing from cube to rhombic dodecahedron to rhombicuboctahedron nanostructures. The observed enhancement is attributed mainly to an increase in their surface polarity as the number of facets increases, which reduces the Coulomb interaction of charge carriers, thereby suppressing Auger recombination. Moreover, Auger recombination rate constants obtained from the time-gated photon correlation studies exhibited a discernible decrease as the number of facets increased. These findings underscore the significance of facet engineering in fine-tuning biexciton emission in metal halide perovskite nanocrystals.

10aAuger recombination10abiexciton emission10aCs-133 MAS NMR spectroscopy10aPerovskites10atime-gatedphoton correlation1 aTitus, Timi1 aVishnu, Krishnan1 aGarai, Arghyadeep1 aDutta, Sumit, Kumar1 aSandeep, Kuttysankaran1 aShelke, Ankita1 aAjithkumar, Thalasseril, G.1 aShaji, Anil1 aPradhan, Narayan1 aThomas, George uhttps://library.ncl.res.in/content/biexciton-emission-cspbbr3-nanocrystals-polar-facet-matters08544nas a2200193 4500008004100000245012400041210006900165260000800234300001200242490000600254520782500260100002108085700002308106700002408129700002308153700001908176700003008195856012508225 2024 eng d00aBimetallic and plasmonic Ag and Cu integrated TiO2 thin films for enhanced solar hydrogen production in direct sunlight0 aBimetallic and plasmonic Ag and Cu integrated TiO2 thin films fo cAPR a829-8400 v33 a
Plasmonic metal nanoparticle-integrated mesoporous TiO2 nanocomposites (Ag/TiO2, Cu/TiO2 and Ag–Cu/TiO2), prepared by a simple chemical reduction method, have been demonstrated to show superior activity in thin-film form for solar H2 generation in sunlight. Integration of Ag + Cu on TiO2 significantly enhances the solar H2 production due to the combined SPR effect of both metal species and the possible synergistic interaction among Cu + Ag in Ag–Cu/TiO2. TiAgCu-1 (0.75 wt% Ag and 0.25 wt% Cu on TiO2) showed the highest H2 yield of 6.67 mmol h−1 g−1 and it is 43 times higher than that of bare TiO2. The thin-film form of TiAgCu-1 shows 5 times higher solar H2 production than its powder counterpart. 1 wt% of Ag or Cu on TiO2 shows a H2 yield of 4.6 or 2 mmol h−1 g−1, respectively, which underscores the importance of combined or synergistic effects. The increase in solar H2 generation in Ag–Cu/TiO2 is attributed to factors such as the SPR effect of Cu and Ag, and strong interaction between Ag and Cu. The high photocatalytic efficiency of the TiAgCu-1 thin film is attributed to the large dispersion of metallic species with relatively high Ag/Cu surface atomic ratio, enhanced light absorption, a heterogeneous distribution of Ag and Cu species, and high double layer capacitance. The inter particle mesoporous network increases the interfacial charge transfer and reduces the mass transfer limitations. The plausible photocatalytic reaction mechanism could involve the combination of direct electron transfer from metal (Cu/Ag) to TiO2 as well as the significant field effect due to the Ag–Cu alloy, which is expected to increase the electron excitation locally.
Plasmonic metal nanoparticle integrated mesoporous TiO2 nanocomposites (Ag/TiO2, Cu/TiO2 and Ag–Cu/TiO2), prepared by a simple chemical reduction method, have been demonstrated to show superior activity in thin-film form for solar H2 generation.

 

1 aMani, Sunesh, S.1 aRajendran, Sivaraj1 aArun, Pushkaran, S.1 aVijaykumar, Aparna1 aMathew, Thomas1 aGopinath, Chinnakonda, S. uhttps://library.ncl.res.in/content/bimetallic-and-plasmonic-ag-and-cu-integrated-tio2-thin-films-enhanced-solar-hydrogen02281nas a2200241 4500008004100000022001400041245010600055210006900161260000800230300001600238490000600254520145900260653002301719653001901742653002201761653002201783653002201805653001901827100002701846700001701873700002701890856012201917 2024 eng d a2637-610500aBiobased reusable nonisocyanate polyurethane hot-melt adhesives with potential chemical degradability0 aBiobased reusable nonisocyanate polyurethane hotmelt adhesives w cSEP a11180-111920 v63 a

Hot-melt adhesives (HMAs) derived from renewable resources are always attractive, as they promote less dependence on fossil resources and aid in developing environmentally friendly materials. However, developing sustainable HMAs exhibiting good adhesive performance and biodegradability remains challenging. Herein, reusable biobased HMAs with a high bio content of 88-90%, biodegradability, and tunable adhesive nature were developed as a possible alternative to conventional petroleum-based hot-melt adhesives. Moreover, the structure-property relationship of the product was investigated in detail. By tailoring the monomer composition, NIPU-40 HMA exhibited a good bonding ability with a superior adhesion strength of 6.39 MPa. To the best of our knowledge, this is the highest adhesion strength observed when bonding with an Al substrate among the biobased thermoplastic NIPU-HMAs. Impressively, NIPU-HMAs could display excellent reusability even after three bonding and debonding cycles without a significant drop in the adhesive strength and were found to exhibit good adhesion performance under wet conditions. More importantly, the NIPU-HMAs are prone to degradation under acid-catalyzed conditions. Considering their features, these biobased NIPU thermoplastic hot-melt adhesives offer an opportunity to create environmentally friendly, degradable adhesives that possess excellent adhesive strength and can be reused multiple times.

10aacid degradability10abiobased NIPUs10acyclic carbonates10ahot meltadhesives10alap shearstrength10aspiro-aromatic1 aGhuge, Gorakh, Hiraman1 aTorris, Arun1 aNair, Kiran, Sukumaran uhttps://library.ncl.res.in/content/biobased-reusable-nonisocyanate-polyurethane-hot-melt-adhesives-potential-chemical01085nas a2200349 4500008004100000245009600041210006900137260000900206490000700215653001200222653002300234653001200257653002400269653001600293100001700309700001600326700002700342700002000369700002200389700001800411700001800429700001700447700001900464700001700483700001900500700002200519700001800541700002000559700001900579700001800598856011900616 2024 eng d00aBiocatalysis enables the scalable conversion of biobased furans into various furfurylamines0 aBiocatalysis enables the scalable conversion of biobased furans  cJUL 0 v1510aBiomass10aOMEGA-TRANSAMINASE10aOXIDASE10aReductive amination10aSPECIFICITY1 aGiri, Pritam1 aLim, Seonga1 aKhobragade, Taresh, P.1 aPagar, Amol, D.1 aPatil, Mahesh, D.1 aSarak, Sharad1 aJeon, Hyunwoo1 aJoo, Sangwoo1 aGoh, Younghwan1 aJung, Seohee1 aJang, Yu-Jeong1 aChoi, Seung, Beom1 aKim, Ye, Chan1 aKang, Taek, Jin1 aHeo, Yong-Seok1 aYun, Hyungdon uhttps://library.ncl.res.in/content/biocatalysis-enables-scalable-conversion-biobased-furans-various-furfurylamines00723nas a2200205 4500008004100000022001400041245010400055210006900159260000900228300001600237490000700253653000900260653001500269653001900284653002200303100001600325700003000341700001700371856012900388 2024 eng d a1944-824400aBiocompatible PVAc-g--PLLA acrylate polymers for DLP 3D printing with tunable mechanical properties0 aBiocompatible PVAcgPLLA acrylate polymers for DLP 3D printing wi cOCT  a62594-626050 v1610aacid10acopolymers10apolymerization10aTACONIC ANHYDRIDE1 aPal, Shibam1 aGavhane, Utreshwar, Arjun1 aAsha, S., K. uhttps://library.ncl.res.in/content/biocompatible-pvac-g-plla-acrylate-polymers-dlp-3d-printing-tunable-mechanical-properties02769nas a2200229 4500008004100000245012000041210006900161260000800230300001100238490000800249520195200257653002702209653001502236653003002251653002102281100002102302700001702323700002602340700002002366700002702386856012602413 2024 eng d00aBiodegradable and 3D printable lysine functionalized polycaprolactone scaffolds for tissue engineering applications0 aBiodegradable and 3D printable lysine functionalized polycaprola cMAY a2138160 v1593 a

Tissue engineering (TE) has sparked interest in creating scaffolds with customizable properties and functional bioactive sites. However, due to limitations in medical practices and manufacturing technologies, it is challenging to replicate complex porous frameworks with appropriate architectures and bioactivity in vitro. To address these challenges, herein, we present a green approach that involves the amino acid (L-lysine) initiated polymerization of epsilon-caprolactone (CL) to produce modified polycaprolactone (PCL) with favorable active sites for TE applications. Further, to better understand the effect of morphology and porosity on cell attachment and proliferation, scaffolds of different geometries with uniform and interconnected pores are designed and fabricated, and their properties are evaluated in comparison with commercial PCL. The scaffold morphology and complex internal micro-architecture are imaged by micro-computed tomography (micro-CT), revealing pore size in the range of similar to 300-900 mu m and porosity ranging from 30 to 70 %, while based on the geometry of scaffolds the compressive strength varied from 143 +/- 19 to 214 +/- 10 MPa. Additionally, the degradation profiles of fabricated scaffolds are found to be influenced by both the chemical nature and product design, where Lys-PCL-based scaffolds with better porosity and lower crystallinity degraded faster than commercial PCL scaffolds. According to in vitro studies, Lys-PCL scaffolds have produced an environment that is better for cell adhesion and proliferation. Moreover, the scaffold design affects the way cells interact; Lys-PCL with zigzag geometry has demonstrated superior in vitro vitality (>90 %) and proliferation in comparison to other designs. This study emphasizes the importance of enhancing bioactivity while meeting morphology and porosity requirements in the design of scaffolds for tissue engineering applications.

10aAdditive manufacturing10aAmino acid10aMicro-computed tomography10aPolycaprolactone1 aNaik, Sonali, S.1 aTorris, Arun1 aChoudhury, Namita, R.1 aDutta, Naba, K.1 aNair, Kiran, Sukumaran uhttps://library.ncl.res.in/content/biodegradable-and-3d-printable-lysine-functionalized-polycaprolactone-scaffolds-tissue02841nas a2200241 4500008004100000022001400041245015000055210006900205260000800274300001200282490000700294520195200301653001402253653002802267653002802295653003302323653002702356653001302383100002502396700002302421700002302444856013202467 2024 eng d a0972-152500aBioprocess optimization of penicillium funiculosum NCIM 1228 for improved production and hydrolytic efficiency of cellulases on sugarcane bagasse0 aBioprocess optimization of penicillium funiculosum NCIM 1228 for cFEB a215-2330 v263 a

The cellulase enzyme is currently the world's third largest commercial enzyme. Because of its requirement in various industries like textiles, food, waste management, pharmaceuticals, agriculture, pulp paper, biofuels, and others, its demand curve has increased sharply. The response surface methodology (RSM) approach was employed to optimize media components and process parameters in the current investigation, which successfully increased the cellulase production from Penicillium funiculosum NCIM 1228. Statistical optimization for the hyperproduction of cellulases was conducted using RSM. The Plackett-Burman design (PBD) approach was used to investigate the critical factors of the cellulase production medium. Subsequently, the Box-Behnken design (BBD) method was used to statistically estimate optimum values and conditions that substantially impacted cellulase production. The estimated optimal combinations of parameters for cellulase production were urea (0.2%), CaCl2 (0.2%), MgSO4 (0.05%), peptone (1.5%), microcrystalline cellulose (5.0%), wheat bran (2.5%), corn steep liquor (CSL) (2.5%), KH2PO4 (0.15%), inoculum (10.65%), agitation (157 rpm), pH (5.88), and temperature (29.84 C-degrees). Conclusively, experimental validation under optimal conditions detected an increased production of 3.82- and 3.61-fold in filter paper assay (FPase) and beta-glucosidase, respectively. Additionally, 1.66- and 1.57-fold enhancement in FPase and beta-glucosidase specific activity was observed where an xylanase activity was enhanced by 3.29-fold. Furthermore, the enzyme showed 51.30 per cent hydrolysis efficiency on sugarcane bagasse lignocellulosic biomass (LCB), at a dose of 7 FPase units per g of cellulose. P. funiculosum NCIM 1228 offers the benefit of producing cellulase with an entire cellulolytic system of enzymes that can be synthesized extracellularly, thus acting as a promising biocatalyst for biofuel industries.

10aCellulase10aLignocellulosic biomass10aPenicillium funiculosum10aResponse surface methodology10aSubmerged fermentation10aXylanase1 aChavan, Sambhaji, B.1 aShete, Ashvini, M.1 aDharne, Mahesh, S. uhttps://library.ncl.res.in/content/bioprocess-optimization-penicillium-funiculosum-ncim-1228-improved-production-and-hydrolytic01319nas a2200229 4500008004100000022001400041245015600055210006900211260000900280300001200289490000700301520049200308653002900800653001300829653001400842653001100856100002300867700002600890700002500916700002200941856012600963 2024 eng d a1477-052000aBi(OTf)3-promoted cascade annulation of hydroxy-pyranones and unsaturated γ-ketoesters for the construction of polycyclic bridged pyrano-furopyranones0 aBiOTf3promoted cascade annulation of hydroxypyranones and unsatu cJAN  a496-5000 v223 a

An efficient protocol for constructing complex three dimensional polycyclic bridged chromano-furopyranones and pyrano-furopyranones (closely related to bioactive natural products) via bismuth(III)-catalyzed cascade annulation of hydroxy-pyranones and unsaturated gamma-ketoesters is presented. This process involves intermolecular Michael addition, intramolecular hemiketalization, lactonization, formation of one C-C bond and two C-O bonds, rings, and contiguous stereocenters.

10a1 3-Dicarbonyl Compounds10aalkynols10aCoumarins10aFungus1 aRathod, Akshay, B.1 aBorade, Balasaheb, R.1 aSambherao, Pooja, I.1 aKontham, Ravindar uhttps://library.ncl.res.in/content/biotf3-promoted-cascade-annulation-hydroxy-pyranones-and-unsaturated-%CE%B3-ketoesters02038nas a2200241 4500008004100000245008300041210006900124260000900193300001400202490000700216520124800223100002301471700001901494700002201513700001901535700001801554700002501572700001801597700002601615700001701641700002401658856011401682 2024 eng d00aBismuth ferrite based acetone gas sensor: evaluation of graphene oxide loading0 aBismuth ferrite based acetone gas sensor evaluation of graphene  cJAN  a1367-13760 v143 a

We report a BiFeO3/graphene oxide (BFO/GO) perovskite, synthesized using a CTAB-functionalized glycine combustion route, as a potential material for acetone gas sensing applications. The physicochemical properties of the developed perovskite were analysed using XRD, FE-SEM, TEM, HRTEM, EDAX and XPS. The gas sensing performance was analysed for various test gases, including ethanol, acetone, propanol, ammonia, nitric acid, hydrogen sulphide and trimethylamine at a concentration of 500 ppm. Among the test gases, the developed BFO showed the best selectivity towards acetone, with a response of 61% at an operating temperature of 250 degrees C. All the GO-loaded BFO samples showed an improved gas sensing performance compared with pristine BFO in terms of sensitivity, the response/recovery times, the transient response curves and the stability. The 1 wt% GO-loaded BiFeO3 sensor showed the highest sensitivity of 89% towards acetone (500 ppm) at an operating temperature of 250 degrees C. These results show that the developed perovskites have significant potential for use in acetone gas sensing applications. CTAB-functionalized glycine combustion yields BiFeO3/graphene oxide (BFO/GO) perovskite for acetone gas detection.

1 aGhadage, Pandurang1 aShinde, K., P.1 aNadargi, Digambar1 aNadargi, Jyoti1 aShaikh, Hamid1 aAlam, Mohammad, Asif1 aMulla, Imtiaz1 aTamboli, Mohaseen, S.1 aPark, J., S.1 aSuryavanshi, Sharad uhttps://library.ncl.res.in/content/bismuth-ferrite-based-acetone-gas-sensor-evaluation-graphene-oxide-loading02862nas a2200205 4500008004100000245017400041210006900215260000800284300001200292490000700304520205400311100001702365700002502382700001902407700002502426700002202451700002802473700002402501856013102525 2024 eng d00aBlock copolymer self-assembly: exploitation of hydrogen bonding for nanoparticle morphology control via incorporation of triazine based comonomers by RAFT polymerization0 aBlock copolymer selfassembly exploitation of hydrogen bonding fo cOCT a24011290 v203 a

Synthesis of polymeric nanoparticles of controlled non-spherical morphology is of profound interest for a wide variety of potential applications. Self-assembly of amphiphilic diblock copolymers is an attractive bottom-up approach to prepare such nanoparticles. In the present work, RAFT polymerization is employed to synthesize a variety of poly(N,N-dimethylacrylamide)-b-poly[butyl acrylate-stat-GCB] copolymers, where GCB represents vinyl monomer containing triazine based Janus guanine-cytosine nucleobase motifs featuring multiple hydrogen bonding arrays. Hydrogen bonding between the hydrophobic blocks exert significant influence on the morphology of the resulting nanoparticles self-assembled in water. The Janus feature of the GCB moieties makes it possible to use a single polymer type in self-assembly, unlike previous work exploiting, e.g., thymine-containing polymer and adenine-containing polymer. Moreover, the strength of the hydrogen bonding interactions enables use of a low molar fraction of GCB units, thereby rendering it possible to use the present approach for copolymers based on common vinyl monomers for the development of advanced nanomaterials.

1 aHaque, Farah1 aThompson, Steven, W.1 aIshizuka, Fumi1 aKuchel, Rhiannon, P.1 aSingh, Dharmendra1 aSanjayan, Gangadhar, J.1 aZetterlund, Per, B. uhttps://library.ncl.res.in/content/block-copolymer-self-assembly-exploitation-hydrogen-bonding-nanoparticle-morphology-control02427nas a2200277 4500008004100000022001400041245012800055210007000183260000900253490000700262520147600269653003801745653001001783653001801793653001701811653000901828100002201837700001801859700003001877700002401907700002001931700002001951700001801971700003001989856013002019 2024 eng d a0947-653900aBoron- and oxygen-doped π-extended helical nanographene with circularly polarised thermally activated delayed fluorescence0 aBoron and oxygendoped πextended helical nanographene with circul cAPR 0 v303 a

Helical nanographenes have garnered substantial attention owing to their finely adjustable optical and semiconducting properties. The strategic integration of both helicity and heteroatoms into the nanographene structure, facilitated by a boron-oxygen-based multiple resonance (MR) thermally activated delayed fluorescence (TADF), elevates its photophysical and chiroptical features. This signifies the introduction of an elegant category of helical nanographene that combines optical (TADF) and chiroptical (CPL) features. In this direction, we report the synthesis, optical, and chiroptical properties of boron, oxygen-doped pi-extended helical nanographene. The pi-extension induces distortion in the DOBNA-incorporated nanographene, endowing a pair of helicenes, (P)-B2NG, and (M)-B2NG exhibiting circularly polarized luminescence with glum of -2.3x10-3 and +2.5x10-3, respectively. B2NG exhibited MR-TADF with a lifetime below 5 mu s, and a reasonably high fluorescence quantum yield (50 %). Our molecular design enriches the optical and chiroptical properties of nanographenes and opens up new opportunities in multidisciplinary fields. B, O-doped pi-extended helical nanographene has been explored for its optical and chiroptical properties. The presence of helical and nanographene segments stabilize the helicity and imparts tunable optical properties. Helical nanographene display circularly polarized thermally activated delayed fluorescence. image

10acircularly polarized luminescence10aDOBNA10adual emission10ananographene10aTADF1 aVenugopal, Geethu1 aKumar, Viksit1 aJadhav, Ashok, Badrinaray1 aDongre, Sangram, D.1 aKhan, Abujunaid1 aGonnade, Rajesh1 aKumar, Jatish1 aBabu, Sukumaran, Santhosh uhttps://library.ncl.res.in/content/boron-and-oxygen-doped-%CF%80-extended-helical-nanographene-circularly-polarised-thermally01025nas a2200301 4500008004100000022001400041245010800055210006900163260000800232300001100240490000800251653002400259653002800283653003000311100002700341700001800368700002000386700002600406700002200432700002600454700001800480700001800498700001800516700002500534700001500559700001900574856013000593 2024 eng d a0927-796X00aBreaking the inactivity of MXenes to drive Ampere-level selective oxygen evolution reaction in seawater0 aBreaking the inactivity of MXenes to drive Amperelevel selective cSEP a1008350 v16010a2D heterostructures10aMetal-organic framework10aOxygen Evolution Reaction1 aGbadamasi, Sharafadeen1 aLoomba, Suraj1 aHaris, Muhammad1 aKhan, Muhammad, Waqas1 aMaibam, Ashakiran1 aMousavi, Seyed, Mahdi1 aMahmud, Sofiu1 aThomsen, Lars1 aTadich, Anton1 aBabaRao, Ravichandar1 aXian, Jian1 aMahmood, Nasir uhttps://library.ncl.res.in/content/breaking-inactivity-mxenes-drive-ampere-level-selective-oxygen-evolution-reaction-seawater02936nas a2200301 4500008004100000022001400041245013700055210006900192260000800261490000700269520184900276653003702125653001802162653004202180653003002222653003002252653001702282100002002299700003002319700002902349700002502378700001802403700002002421700001802441700002602459700002402485856012502509 2024 eng d a1613-681000aBreaking the Pt electron symmetry and OH spillover towards ptir active center for performance modulation in direct ammonia fuel cell0 aBreaking the Pt electron symmetry and OH spillover towards ptir  cDEC0 v203 a

The growing interest in low-temperature direct ammonia fuel cells (DAFCs) arises from the utilization of a carbon-neutral ammonia source; however, DAFCs encounter significant electrode overpotentials due to the substantial energy barrier of the *NH2 to *NH dehydrogenation, compounded by the facile deactivation by *N on the Pt surface. In this work, a unique catalyst, Pt4Ir@AlOOH/NGr i.e., Pt4Ir/ANGr, is introduced composed of PtIr alloy nanoparticles controllably decorated on the pseudo-boehmite phase of AlOOH-supported nitrogen-doped reduced graphene (AlOOH/NGr) composite, synthesized via the polyol reduction method. The detailed studies on the structural and electronic properties of the catalyst by XAS and VB-XPS reveal the possible electronic modulations. The optimized Pt4Ir/ANGr composition exhibits a significantly improved onset potential and mass activity for AOR. The DFT study confirms the OHad species spillover by AlOOH and Pt4Ir (100) facilitates the conversion of the *NH2 to *NH with minimal energy barriers. Finally, testing of DAFC at the system level using a membrane electrode assembly (MEA) with Pt4Ir/ANGr as the anode catalyst, demonstrating the suitability of the catalyst for its practical applications. This study thus uncovers the potential of the Pt4Ir catalyst in synergy with ANGr, largely addressing the challenges in hydrogen transportation, storage, and safety within DAFCs. In this article, a versatile catalyst, Pt4Ir/ANGr, is introduced composed of PtIr alloy nanoparticles decorated on AlOOH/NGr composite. The AlOOH provides an -OH-rich surface conducive to the facile adsorption and decoration of the PtIr alloy nanoparticles and OHad species spillover from AlOOH to the Pt4Ir active center during AOR, further validating the higher experimental activity obtained in Pt4Ir/ANGr. image

10aammonia oxidation reaction (AOR)10ad-band center10adensity functional theory (DFT) study10adirect ammonia fuel cells10ahydroxyl spillover effect10aXAS analysis1 aBarik, Sidharth1 aKharabe, Geeta, Pandurang1 aSamal, Pragnya, Paramita1 aUrkude, Rajashri, R.1 aKumar, Sachin1 aYoyakki, Athira1 aVinod, C., P.1 aKrishnamurty, Sailaja1 aKurungot, Sreekumar uhttps://library.ncl.res.in/content/breaking-pt-electron-symmetry-and-oh-spillover-towards-ptir-active-center-performance02246nas a2200181 4500008004100000022001400041245007300055210006900128260000800197300001100205490000700216520166400223100002001887700002201907700002101929700001701950856009701967 2024 eng d a1359-029400aBridging the gap: an investigation of biosurfactants-polymer systems0 aBridging the gap an investigation of biosurfactantspolymer syste cAUG a1018060 v723 a

Biosurfactants (BSs) have been extensively researched due to their potential applications in various fields, including textiles, cosmetics, pharmaceuticals, agriculture, and oil remediation. These BSs possess a diverse range of physical, chemical, and biological properties. In recent years, researchers have combined these biosurfactants with both natural and synthetic polymers, resulting in the development of advanced material systems that exhibit a unique combination of properties. This review focuses on highlighting the recent advancements in these biosurfactant-polymer material systems and identifies existing gaps in the literature. The combination of biosurfactants with polymers has led to the formation of interpenetrated hydrogels, films, chemically modified surfaces, vesicles, functionalized nanofiber nonwoven mats, nano-formulations, and nano-assemblies. Some studies have also investigated the interactions between biosurfactants and polymer molecules. In most cases, non-specific, non-covalent interactions, such as electrostatic interactions, hydrogen bonding, and hydrophobic interactions have been found to govern the properties of these systems. Moreover, promising results have been achieved through the covalent modification of polymer surfaces, followed by functionalization using biosurfactant molecules. The literature demonstrates that these advanced materials could find applications in various fields, including drug delivery, bioremediation, biomedical materials, and as antimicrobial agents. These findings indicate the promising potential of biosurfactant-polymer systems for future advancements in these areas.

1 aAbhyankar, Isha1 aHirlekar, Swarali1 aPrabhune, Asmita1 aNisal, Anuya uhttps://library.ncl.res.in/content/bridging-gap-investigation-biosurfactants-polymer-systems01990nas a2200205 4500008004100000022001400041245015200055210007200207260000900279300001600288490000600304520119700310100002501507700002401532700002101556700001701577700002601594700002201620856014201642 2024 eng d a2470-134300aBronsted acid-promoted cyclodimerization of α,β-unsaturated γ-ketoesters: construction of fused pyrano-ketal-lactones and γ-ylidene-butenolides0 aBronsted acidpromoted cyclodimerization of αβunsaturated γketoes cAPR  a19859-198780 v93 a

Unprecedented MsOH-promoted diastereoselective cascade dimerization and intramolecular lactonization of readily accessible alpha,beta-unsaturated gamma-ketoesters are presented. The results obtained in this work, control experiments, and density functional theory (DFT) calculations suggested that the initial enolization and E to Z isomerization/equilibration of olefin (C=C) of substrate alpha,beta-unsaturated gamma-ketoesters give a Z-isomer preferentially over an E-isomer. Subsequently, the Z-isomer undergoes intermolecular annulation with alpha,beta-unsaturated gamma-ketoesters via domino Michael addition/ketalization/lactonization steps to furnish fused tetracyclic pyrano-ketal-lactone. However, the Z-isomer prefers intramolecular trans-esterification in a competing pathway and gives bicyclic gamma-ylidene-butenolide. The key features of this work include simple Br & oslash;nsted acid catalysis, the formation of three bonds, two rings, and three contiguous stereogenic centers in a single step, DFT calculations, and the assignment of relative stereochemistry through X-ray diffraction (XRD) and two-dimensional (2D) nuclear magnetic resonance (NMR) analyses.

1 aVinodkumar, Ramavath1 aNakate, Ashwini, K.1 aSharma, Himanshu1 aVanka, Kumar1 aKrishna, Gamidi, Rama1 aKontham, Ravindar uhttps://library.ncl.res.in/content/bronsted-acid-promoted-cyclodimerization-%CE%B1%CE%B2-unsaturated-%CE%B3-ketoesters-construction-fused01776nas a2200217 4500008004100000022001400041245017200055210007000227260000900297300001400306490000700320520092900327100002701256700003301283700002101316700002401337700001801361700001701379700002501396856013701421 2024 eng d a1477-052000aBrønsted acid- and Ni(ii)-catalyzed C-H oxidation/rearrangement of cyclotriveratrylenes (CTVs) to cyclic and acyclic quinones as potential anti-cancer agents0 aBrønsted acid and Nisciisccatalyzed CH oxidationrearrangement of cJAN  a1038-10460 v223 a

This paper describes a simple and practical protocol for the direct synthesis of acyclic and cyclic quinone derivatives via an acid-promoted nickel(ii)-catalyzed inner rim C-H oxidation of cyclotriveratrylene (CTV) and its analogues. The cyclic quinone derivatives resulted from trimethoxy-cyclotriveratrylene (TCTV) through C-C bond formation via intramolecular ipso substitution followed by subsequent anionic rearrangement containing stereo-vicinal quaternary centers. The DFT calculations strongly support the experimental findings and reveal the role of Bronsted acids in the C-H bond activation of CTV. All the newly synthesized compounds were screened for their in vitro anti-cancer activity using colorimetric SRB assay analysis. Among them, compounds 3a, 3d, 3h, 4a, 4b, 4c and 4e exhibited moderate anticancer activity against A549, HCT-116, PC-3, MDA-MB-231, HEK-293 and SW620 human cancer cell lines.

1 aDarole, Ratanamala, S.1 aChoudhary, Shailendra, Singh1 aSharma, Himanshu1 aMali, Bhupendra, P.1 aGopu, Booblan1 aVanka, Kumar1 aSenthilkumar, Beeran uhttps://library.ncl.res.in/content/br%C3%B8nsted-acid-and-niii-catalyzed-c-h-oxidationrearrangement-cyclotriveratrylenes-ctvs-cyclic02210nas a2200217 4500008004100000022001400041245011100055210006900166260000800235300001100243490000800254520145000262653002801712653001701740653002501757653001301782100001901795700002501814700002301839856013001862 2025 eng d a0141-813000aBacterial nanocellulose (BNC) produced from sorbitol as a sustainable nano-filter for oil-water separation0 aBacterial nanocellulose BNC produced from sorbitol as a sustaina cJAN a1379530 v2843 a

Oil spillage is one of the serious problems for sustainable environment. Bacterial nanocellulose (BNC), a hydrophilic and highly porous material holds a promising material for oil-water separation from contaminants. In the present work, a hydrophilic BNC produced from a sorbitol as the carbon source demonstrated the unique porous symmetrical arrangement having an oleophilic property. The BNC membrane obtained showed the highest water holding capacity (WHC) of approximate to 147 gg 1 . The Brunauer-Emmett-Teller (BET) analysis of BNC revealed the unique characteristics of isothermic patterns, having macro sized pores with diameter of 121.3 nm and surface area of 40.6m2/g, which plays a vital role in separation of oil from water by allowing passage of only water through it. The separation efficiency of BNC membrane produced after 5th day of incubation has showed 99.0 % oil removal compared to 10 and 15th day incubated BNC membranes. a CFD model to investigate the possibilities of BNC membranes and clarify the dynamics of oil-water separation. The nanostructured network of BNC offers a tortuous path for oil molecules while allowing rapid permeation of water, leading to high separation selectivity and flux. Although BNC has been previously studied for oil water separation, this study provides new insights into the use of wet BNC membranes into its pristine state with sorbitol as carbon source for this application.

10abacterial nanocellulose10aMacro porous10aOil-water separation10aSorbitol1 aJagtap, Ashish1 aKrushnatray, Ajinkya1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/bacterial-nanocellulose-bnc-produced-sorbitol-sustainable-nano-filter-oil-water-separation02695nas a2200229 4500008004100000022001400041245010800055210006900163260000800232300001100240490000800251520188100259653002702140653002802167653001602195653003102211100002702242700001902269700002302288700002402311856013002335 2025 eng d a0141-813000aBacterial nanocellulose enhances osteogenic activity of Vat-based 3D-bio-printed silk fibroin hydrogels0 aBacterial nanocellulose enhances osteogenic activity of Vatbased cNOV a1475320 v3283 a

Tissue engineering, particularly leveraging three-dimensional (3D) bioprinting, is emerging as a transformative solution to repair critical-size bone defects. However, identifying suitable biomaterials remains a key technological bottleneck in the field. Toward this broader goal, this study explored a composite bioink containing photocurable silk fibroin (SF) and bacterial nanocellulose (BNC) for fabricating scaffolds for bone tissue engineering (BTE) by 3D bioprinting using digital light projection (DLP). We prepared scaffolds with 0, 0.25, and 0.75 wt% BNC and characterized their physicochemical properties (degradation, viscoelasticity, porosity, compressive strength). We assessed samples in simulated body fluid (SBF) after 14 days to evaluate biomineralization. Additionally, using MC3T3-E1 preosteoblast cells, we examined cell viability, metabolic activity, proliferation, and osteogenic potential through alkaline phosphatase (ALP), Alizarin Red S (ARS), von Kossa, hematoxylin and eosin (H&E), and Picrosirius Red assays. The optimized bioinks produced hydrogels with controlled degradation, tunable viscoelasticity, interconnected pores, and significantly improved compressive strength. Specifically, 10 % methacrylated-silk with 0.75 % BNC (Silk-MA/0.75BNC) showed superior mechanical properties compared to 10 % Silk-MA or 10 % Silk-MA with 0.25 % BNC (Silk-MA/0.25BNC). In vitro studies confirmed enhanced biomineralization with Silk-MA/0.75BNC, increased calcium deposits, and improved cell viability and metabolic activity with BNC incorporation. Hence, the 3D-bioprinted composite scaffolds were shown to effectively support cell proliferation, with the 0.75 % BNC bioink significantly stimulating osteogenic markers. These results underscore the potential of Silk-MA/BNC composite bioinks for advanced 3D bioprinting of BTE constructs.

10aAdditive manufacturing10abacterial nanocellulose10aBone repair10aMethacrylated silk fibroin1 aWaidi, Yusuf, Olatunji1 aJagtap, Ashish1 aDastagar, Syed, G.1 aChatterjee, Kaushik uhttps://library.ncl.res.in/content/bacterial-nanocellulose-enhances-osteogenic-activity-vat-based-3d-bio-printed-silk-fibroin01218nas a2200169 4500008004100000245011200041210006900153260000900222300001600231490000700247520054900254100003300803700002700836700002600863700002500889856013400914 2025 eng d00aBase mediated approach for the synthesis of deoxybenzoins using γ-aryl-β-ketoesters and benzoyl chlorides0 aBase mediated approach for the synthesis of deoxybenzoins using  cSEP  a32424-324300 v153 a

This study introduces a one-pot, transition metal-free strategy for synthesizing deoxybenzoins, overcoming the challenges of conventional methods. The reaction involves dual acylation of gamma-aryl beta-keto esters using K2CO3 in dioxane at 90 degrees C, followed by a concerted transformation to form deoxybenzoin. The protocol operates under mild conditions, tolerates a broad range of substrates, and produces minimal by-products, making it a practical, scalable alternative for accessing pharmaceutically relevant deoxybenzoins.

1 aChoudhary, Shailendra, Singh1 aDarole, Ratanamala, S.1 aKrishna, Gamidi, Rama1 aSenthilkumar, Beeran uhttps://library.ncl.res.in/content/base-mediated-approach-synthesis-deoxybenzoins-using-%CE%B3-aryl-%CE%B2-ketoesters-and-benzoyl00980nas a2200157 4500008004100000022001400041245007100055210006900126260000900195300001200204490000700216520044300223100003300666700002000699856010300719 2025 eng d a1477-052000aBase-mediated denitrative C3-alkylation of quinoxaline derivatives0 aBasemediated denitrative C3alkylation of quinoxaline derivatives cJAN  a303-3070 v233 a

We have developed a novel base-mediated method for the selective C3-alkylation of quinoxalin-2(1H)-one and N-protected quinoxalin-2(1H)-one using inexpensive, unactivated nitroalkanes. This approach tolerates a wide range of functional groups and supports the synthesis of various bioactive compounds. Gram-scale reactions demonstrate the scalability of the method. The proposed mechanism was validated by control experiments.

1 aPansare, Vaibhav, Ramachandr1 aBarsu, Nagaraju uhttps://library.ncl.res.in/content/base-mediated-denitrative-c3-alkylation-quinoxaline-derivatives02478nas a2200229 4500008004100000022001400041245012900055210006900184260000800253300001100261490000800272520167100280653002301951653002401974653001701998653002802015100001802043700002202061700002002083700002002103856012502123 2025 eng d a1093-326300aBenchmarking a dual-scale hybrid simulation framework for small globular proteins combining the CHARMM36 and Martini2 models0 aBenchmarking a dualscale hybrid simulation framework for small g cMAR a1089260 v1353 a

Multi-scale models in which varying resolutions are considered in a single molecular dynamics simulation setup are gaining importance in integrative modeling. However, combining atomistic and coarse-grain resolutions, especially for coarse-grain force fields derived from top-down approaches, have not been well explored. In this study, we have implemented and tested a dual-resolution simulation approach to model globular proteins in atomistic detail (represented by the CHARMM36 model) with the surrounding solvent in Martini2 coarse-grain detail. The hybrid scheme considered is an extension of a model implemented earlier for mainly lipid and water molecules. We have considered a set of small globular proteins and have extensively compared to atomistic benchmark simulations as well as a host of experimental observables. We show that the protein structural dynamics sampled in the hybrid scheme is robust, and the intra-protein contact maps are reproduced, despite increased fluctuations of the loop regions. A good match is observed with experimental small angle X-ray scattering (SAXS) and NMR observables, such as chemical shifts and (3)J((HN-H alpha))-coupling, with the best match obtained for the chemical shifts. However, deviations are observed in the water dynamics and protein-water interactions which we attribute to the limitation of solvent screening in the coarse-grain force field. The computational speed-up achieved is about 2-3 times compared to an all-atom system. Overall, the hybrid model is able to retain the main features of the underlying atomistic conformational landscape with a two-fold speed-up in computational cost.

10aHybrid simulations10aMartini force-field10aMixed models10aMulti-scale simulations1 aYadav, Manjul1 aKharche, Shalmali1 aPrakash, Shikha1 aSengupta, Durba uhttps://library.ncl.res.in/content/benchmarking-dual-scale-hybrid-simulation-framework-small-globular-proteins-combining02213nas a2200265 4500008004100000022001400041245014100055210006900196260000800265300001600273490000700289520133700296653001401633653001001647653002001657653000801677653001801685653000801703653002001711100001501731700002601746700001901772700003101791856012501822 2025 eng d a1944-824400aBifunctional nanoelectrocatalyst: semi-amorphous cobalt tungstate-based nanocomposites surpassing amorphous and crystalline counterparts0 aBifunctional nanoelectrocatalyst semiamorphous cobalt tungstateb cFEB a15319-153320 v173 a

Electrocatalytic water splitting to hydrogen and oxygen is considered to be one of the significant routes for future renewable energy conversion. Therefore, the development of unique, efficient, and cost-effective bifunctional electrocatalysts, which can perform both hydrogen evolution reaction (HER) and oxygen evolution reaction (OER), is critical for the accessible utilization of renewable energy. The increasing overall efficiency of the water-splitting reaction is pivotal for sustainable energy conversion and hydrogen production. Herein, we present a facile solution-based synthesis route to control the crystallinity of the cobalt tungstate (CoWO4) nanomaterials to amorphous, semiamorphous, and crystalline phases and further exploit these nanomaterials as bifunctional electrocatalysts for the HER, OER, and overall electrochemical water splitting reaction. Notably, the semiamorphous cobalt tungstate nanomaterials show a remarkable surface area of approximately 150 m(2)/g, the highest reported for CoWO4-based materials, and demonstrated superior activity for both the OER and HER and outstanding stability over the amorphous and crystalline counterparts. Notably, the SemiAmp||SemiAmp electrolyzer demonstrated a better performance for overall water splitting than the commercial RuO2||Pt/C electrolyzer.

10aamorphous10aCoWO410aelectrocatalyst10aHER10aNanocomposite10aOER10awater splitting1 aDev, Sahil1 aNagappan, Sreenivasan1 aKundu, Subrata1 aMukherjee, Shatabdi, Porel uhttps://library.ncl.res.in/content/bifunctional-nanoelectrocatalyst-semi-amorphous-cobalt-tungstate-based-nanocomposites02443nas a2200313 4500008004100000022001400041245012100055210006900176260000800245490000600253520146100259653000801720653001701728653000801745653001501753653000801768653001501776100001801791700002001809700003001829700001701859700002201876700001401898700002201912700002101934700002101955700002401976856012902000 2025 eng d a2366-748600aBinder-free in situ interface reconstruction of NiMoO4 nanorods over Ni(OH)2 nanosheets for efficient urea oxidation0 aBinderfree in situ interface reconstruction of NiMoO4 nanorods o cDEC0 v93 a

Replacing the energy-intensive oxygen evolution reaction (OER) with the urea oxidation reaction (UOR) in electrochemical water splitting offers simultaneous green hydrogen production and urea-rich wastewater oxidation, enhancing energy efficiency and economic viability. In this study, a non-noble metal-based binder-free NiMoO4/Ni(OH)2/NF electrocatalyst is developed, featuring NiMoO4 nanorods anchored on Ni(OH)2 nanosheets. This unique morphology facilitates a highly active in situ reconstructed interface, delivering a current density of 134 mA cm-2 at 1.40 V (vs RHE) in 1 m KOH with 0.33 m urea, significantly outperforming its individual components. The catalyst demonstrates excellent stability over 50 h at 30 mA cm-2. When integrated into an anion exchange membrane urea electrolyser (13 cm2 area) with Pt@C/NF as the HER cathode, the system achieves 192 mA cm-2 at 1.60 V. The post-UOR studies confirm the presence of an amorphous NiMoO4-crystalline Ni(OH)2 interface, which plays a key role in enhancing the availability of the active sites to enhance the UOR performance. The improved electrochemical performance of the engineered catalyst can be ascribed to the in situ reconstructed amorphous-crystalline interface, optimal hydrophilicity, reduced charge transfer resistance, and the distinct morphology. This strategy offers a promising pathway for developing highly active electrocatalysts for energy conversion applications.

10aAEM10aelectrolyser10aOWS10atomography10aUOR10awastewater1 aKumar, Yogesh1 aBarik, Sidharth1 aKharabe, Geeta, Pandurang1 aTorris, Arun1 aSingh, Mayank, U.1 aMohan, S.1 aGalave, Chaitanya1 aTekawadia, Jyoti1 aKanawade, Rajesh1 aKurungot, Sreekumar uhttps://library.ncl.res.in/content/binder-free-situ-interface-reconstruction-nimoo4-nanorods-over-nioh2-nanosheets-efficient02358nas a2200313 4500008004100000022001400041245014700055210006900202260000900271300001200280490000800292520132300300653001501623653001101638653001601649653001201665653000801677653001501685100002201700700002401722700002301746700002001769700002801789700002101817700002601838700002201864700002601886856013201912 2025 eng d a1226-086X00aBinderless low silica X zeolite for methane separation from binary CO2/CH4 biogas stream: A comprehensive experimental and computational study0 aBinderless low silica X zeolite for methane separation from bina cSEP  a705-7190 v1493 a

This study presents the synthesis of binderless Na-LSX (B-Na-LSX) and binderless NaK-LSX (B-NaK-LSX). XRD, SEM, EDS, and N-2 physisorption were used for characterization of samples. Computational modelling of prepared sorbent was performed for the fundamental understanding of zeolite topology and adsorption behaviour. The breakthrough experiments are used to evaluate the adsorption capacities on CO2/CH4 (40/60 vol%) binary biogas stream. The results were compared with commercial Na-LSX (C-Na-LSX). The breakthrough adsorption capacity of B-Na-LSX was 3.08 mmol g(-1) and 0.29 mmol g(-1) of CO2 and CH4, respectively, at 300 K and 1 bar. CO2 sorption capacity of B-Na-LSX was similar to 11 % and similar to 17 % higher than B-NaK-LSX (2.77 mmol g(-1)) and C-Na-LSX (2.56 mmol g(-1)), respectively. DFT study reveals that the higher adsorption of CO2 over CH4 was attributed to higher charge transfer from CO2 to zeolite framework. Dual-bed six-step Pressure Swing Adsorption (PSA) was performed on B-Na-LSX at 6 bar. similar to 232 cycles were run with above 98 % of CH4 purity and similar to 74 % of recovery. The life-cycle test of sorbent was studied. It was implied that the designed sorbent is effective to produce the high purity of CH4 and can be utilized for a longer period for CH4 production.

10aBinderless10aBiogas10aLSX zeolite10amethane10aPSA10aSeparation1 aYadav, Tushar, R.1 aShrotri, Aadesh, R.1 aKate, Pranjali, N.1 aDevasia, George1 aNiphadkar, Prashant, S.1 aMali, Nilesh, A.1 aKrishnamurty, Sailaja1 aBokade, Vijay, V.1 aNandanwar, Sachin, U. uhttps://library.ncl.res.in/content/binderless-low-silica-x-zeolite-methane-separation-binary-co2ch4-biogas-stream-comprehensive02083nas a2200241 4500008004100000022001400041245015700055210006900212260000900281300001200290490000700302520118800309653002201497653002301519653002101542653002801563653002301591100002501614700002401639700002701663700002601690856012501716 2025 eng d a1060-132500aBio-based poly(isobornyl acrylate)-b-(citronellyl methacrylate) block copolymers by PET-RAFT polymerization: thiol-ene modification and adhesion studies0 aBiobased polyisobornyl acrylatebcitronellyl methacrylate block c cSEP  a728-7360 v623 a

In this study, poly(isobornyl acrylate)-b-poly(citronellyl methacrylate) (PIBA-b-PCMA) block copolymers were synthesized with controlled molecular weights and low dispersity using photoinduced electron transfer-reversible addition-fragmentation chain transfer (PET-RAFT) polymerization of isobornyl acrylate (IBA) and citronellyl methacrylate (CMA), both terpene-derived monomers. A series of block copolymers was obtained by varying the chain length of PCMA block. Kinetics study of the polymerization of IBA and CMA using 4-cyano-(((dodecylthio)carbonothioyl)thio)pentanoic acid (CDTPA) as chain transfer agent showed a linear plot of ln[M]0/[M]t vs time indicating first order reaction. Distinct glass transition temperatures were observed for the individual blocks and enhanced thermal stability was seen with increasing PCMA content. Post-polymerization modification of the polymer was demonstrated using thiol-ene reaction with the vinyl group in the pendent citronellyl moiety. Adhesion performance, evaluated through lap shear test on aluminum substrates, showed that the block copolymers achieved peak shear strengths of 1.74 MPa at a 38:62 PIBA:PCMA mole ratio.

10aadhesion strength10aBio-based polymers10ablock copolymers10aPET-RAFT polymerization10aThiol-ene reaction1 aHetkale, Pragati, M.1 aPurohit, Poorvi, M.1 aNair, Kiran, Sukumaran1 aAmbade, Ashootosh, V. uhttps://library.ncl.res.in/content/bio-based-polyisobornyl-acrylate-b-citronellyl-methacrylate-block-copolymers-pet-raft02537nas a2200265 4500008004100000022001400041245009800055210006900153260000800222490000700230520165800237653001701895653003501912653001601947653002101963100002201984700002202006700001702028700001702045700002102062700002002083700002002103700001902123856012902142 2025 eng d a0935-964800aBioinspired nanochitin-based porous constructs for light-driven whole-cell biotransformations0 aBioinspired nanochitinbased porous constructs for lightdriven wh cJUN0 v373 a

Solid-state photosynthetic cell factories (SSPCFs) are a new production concept that leverages the innate photosynthetic abilities of microbes to drive the production of valuable chemicals. It addresses practical challenges such as high energy and water demand and improper light distribution associated with suspension-based culturing; however, these systems often face significant challenges related to mass transfer. The approach focuses on overcoming these limitations by carefully engineering the microstructure of the immobilization matrix through freeze-induced assembly of nanochitin building blocks. The use of nanochitins with optimized size distribution enabled the formation of macropores with lamellar spatial organization, which significantly improves light transmittance and distribution, crucial for maximizing the efficiency of photosynthetic reactions. The biomimetic crosslinking strategy, leveraging specific interactions between polyphosphate anions and primary amine groups featured on chitin fibers, produced mechanically robust and wet-resilient cryogels that maintained their functionality under operational conditions. Various model biotransformation reactions leading to value-added chemicals are performed in chitin-based matrix. It demonstrates superior or comparable performance to existing state-of-the-art matrices and suspension-based systems. The findings suggest that chitin-based cryogel approach holds significant promise for advancing the development of solid-state photosynthetic cell factories, offering a scalable solution to improve the efficiency and productivity of light-driven biotransformation.

10abiocatalysis10alight-driven biotransformation10ananochitins10aporous materials1 aArumughan, Vishnu1 aMedipally, Hitesh1 aTorris, Arun1 aLeva, Tuukka1 aGrimm, Hanna, C.1 aTammelin, Tekla1 aKourist, Robert1 aKontturi, Eero uhttps://library.ncl.res.in/content/bioinspired-nanochitin-based-porous-constructs-light-driven-whole-cell-biotransformations02259nas a2200277 4500008004100000245015100041210006900192260000800261300001600269490000600285520129900291653001801590653002801608653000801636653002001644653003001664653002401694100002001718700001901738700001801757700002101775700001701796700001701813700002001830856013101850 2025 eng d00aBioinspired synthesis of bridged isochromane fused pyrazoles by a silver catalyzed cascade reaction and its application for antibacterial activity0 aBioinspired synthesis of bridged isochromane fused pyrazoles by  cAUG a4184–41950 v53 a

A stereoselective silver catalyzed one pot vinylogous aldol addition followed by a cascade [4+2] cycloaddition reaction of α-arylidene pyrazolinones to in situ generated isochromenylium ions has been developed, which provides an unprecedented bridged [2.2.2] [3.3.1] pentacyclic [5-6-6-6-6] skeleton consisting of an isochroman, chroman, and a pyrazole unit in one molecule with good to high yields as a single diastereomer. This method offers mild reaction conditions, wide substrate compatibility, excellent scalability and easy derivatization. A DFT study was carried out to clarify the reaction mechanism. It was exciting to observe that the unprecedented bridged isochromans synthesized here have shown excellent selectivity toward Gram-positive and Gram-negative bacteria. We demonstrate that while some structures are broad spectrum antibacterial there are two distinct structures that can be explored for selective activity.

10aAntibacterial10aBridged [2.2.2] [3.3.1]10aROS10aStereoselective10aVinylogous aldol addition10a[4+2] cycloaddition1 aSingh, Bhuvnesh1 aTewari, Shreya1 aKaur, Manleen1 aSharma, Himanshu1 aVanka, Kumar1 aSingh, Neetu1 aSingh, Ravi, P. uhttps://library.ncl.res.in/content/bioinspired-synthesis-bridged-isochromane-fused-pyrazoles-silver-catalyzed-cascade-reaction01788nas a2200217 4500008004100000022001400041245014800055210006900203260000900272300001600281490000700297520098100304100002001285700002001305700003001325700001601355700002401371700002201395700002501417856012801442 2025 eng d a2041-652000aBis-subporphyrin-like boron(iii) complexes derived from indolo[2,3-a]carbazole-based cyclic bis-BODIPYs exhibiting persistent helical chirality0 aBissubporphyrinlike boroniii complexes derived from indolo23acar cOCT  a19164-191710 v163 a

Herein, we report the synthesis of 11,12-dihydroindolo[2,3-a]carbazole (InC) 1 incorporating expanded porphyrinoid 8, and its boron(iii) complexes (8BF2 and 8BF). These macrocycles exhibit a `figure-eight' conformation that exists as a pair of helical enantiomers (P,P and M,M), confirmed by X-ray crystallographic analysis. The `figure-eight' structures are stabilized by intramolecular hydrogen bonds such as NH & ctdot;N and NH & ctdot;F interactions. The synthesized macrocycles demonstrate excellent stability under ambient conditions. Further, the helically-locked structure 8BF exhibited closely held InC units with a distance of 6.17 & Aring; and enabled facile optical resolution using chiral HPLC. Circular dichroism spectroscopy of enantiopure 8BF shows the Cotton effect at 692 nm extending to the near-IR region with Delta epsilon up to 150 M-1 cm-1. The chiral induction of racemic macrocycle 8 was employed using enantiopure mandelic acids.

1 aSpergen, Aswini1 aBal, Pranav, V.1 aShameem, Asif, Khan Shame1 aJoshy, Arun1 aNambiar, Anjana, P.1 aAndrews, Alex, P.1 aSabapathi, Gokulnath uhttps://library.ncl.res.in/content/bis-subporphyrin-boroniii-complexes-derived-indolo23-acarbazole-based-cyclic-bis-bodipys03165nas a2200157 4500008004100000022001400041245011300055210006900168260000800237490000600245520256000251100001702811700002602828700002402854856012902878 2025 eng d a2398-490200aBoosting the CO2 reduction activity of Cu double-atom catalysts through coordination environment engineering0 aBoosting the CO2 reduction activity of Cu doubleatom catalysts t cOCT0 v93 a

Double-atom catalysts (DACs) offer a reasonable and scalable route towards carbon neutrality owing to their efficient catalytic features. However, the challenges associated with the flexibility of their coordination structure usually restrict their full potential as efficient catalysts. Herein, we comprehensively examined the impact of coordination environment regulation on the CO2R activity of Cu2-DACs supported on C, N, or B co-doped graphene using ab initio simulations. We highlighted the marked role of the local coordination sphere of Cu2-DACs in modulating their structural stability and charge transfer characteristics, thereby regulating the adsorption of CO2 and various reaction characteristics. Notably, boron- and carbon-coordinated Cu2 centres (Cu2-BxCy) resulted in remarkably strong CO2 adsorption (-0.65 to -2.31 eV), attributed to their amplified electronic interactions with the CO2 molecule. The weak CO2 binding observed on the carbon-nitrogen- and nitrogen-boron-coordinated Cu2 centres (Cu2-NxCy and Cu2-NxBy) further highlighted the role of the coordination environment in facilitating the versatile binding modes of the key reaction species. The varying CO2 interactions in these systems were further comprehended and supported by a multilevel descriptor () combining both geometric and electronic parameters. This descriptor closely mirrored the DFT results, thereby accentuating its effectiveness as a predictive tool to perfectly model the CO2 interactions on these catalysts. Moreover, the dynamic behaviour in the adsorption modes led to partial breakdown of the conventional linear scaling relations between the key CO2 reduction intermediates (*COOH, *CO, and *HCO). Among the numerous types of investigated electrocatalysts, Cu2-B5C1 emerged as a highly active and selective catalyst for methanol production, with a remarkably low limiting potential of -0.54 V, surpassing the performance of several reported Cu-based systems. Besides, our findings underscored the often-overlooked yet crucial role of explicit solvation, which significantly altered both the potential-determining step and product selectivity. These outcomes emphasized the necessity of including solvation effects in realistic electrochemical modelling. Collectively, this study provides a critical mechanistic insight into better understanding the coordination effect on the CO2R and a robust design strategy for next-generation Cu-based DACs, guiding the development of highly efficient and selective catalysts for CO2 electroreduction.

1 aAmin, Seerat1 aKrishnamurty, Sailaja1 aDar, Manzoor, Ahmad uhttps://library.ncl.res.in/content/boosting-co2-reduction-activity-cu-double-atom-catalysts-through-coordination-environment02013nas a2200181 4500008004100000022001400041245014600055210007000201260000800271300001400279490000700293520133400300100002101634700002201655700001601677700001901693856011901712 2025 eng d a1144-054600aBrønsted basic TMMM-hydrotrope combined catalytic system for the synthesis of diverse dihydropyranochromenes in water at ambient temperature0 aBrønsted basic TMMMhydrotrope combined catalytic system for the  cMAY a8131-81420 v493 a

This work describes the synthesis of a range of bioactive chromenes using a solid Br & oslash;nsted organic base called `tris(morpholinomethyl)mesitylene' (TMMM). TMMM was obtained in high yields by treating tris(bromomethyl)mesitylene (TBMM) with morpholine in the presence of K2CO3 in acetonitrile at RT. It was characterized using 1H NMR and 13C NMR spectroscopy. We developed a Br & oslash;nsted base hydrotrope combined catalytic system (BBHC) by combining TMMM and a hydrotrope (sodium p-toluene sulphonate: NaPTS) for the cascade-type Knoevenagel-Michael addition reactions of various aldehydes and malononitrile with kojic acid, 4-hydroxy coumarin, dimedone, and lawsone in water. The methodology offers diversity-oriented synthesis (DOS) of dihydropyranochromenes in high yields. The BBHC catalytic system showed at least four times recyclability without appreciable loss of its activity. TMMM is an odorless, solid organic base that can be easily handled and is resistant to air and moisture. As TMMM is a solid base, it can reduce evaporation losses, improve safety and allow its easy recovery and reuse. TMMM acts as a powerful Br & oslash;nsted base that activates reactants, while an aqueous solution of NaPTS acts as a hydrotrope that increases the solubility of organic compounds in water.

1 aRajmane, Archana1 aJadhav, Aishwarya1 aPatil, Nita1 aKumbhar, Arjun uhttps://library.ncl.res.in/content/br%C3%B8nsted-basic-tmmm-hydrotrope-combined-catalytic-system-synthesis-diverse