02065nas a2200241   4500008004100000022001400041245008000055210006900135260000800204300000800212490000700220520134200227653001801569653002401587653001101611653001401622653000901636100001801645700001701663700001701680700001901697856010701716       2019                        eng d  a1530-993200aMapping fusogenicity of ciprofloxacin-loaded liposomes with bacterial cells0 aMapping fusogenicity of ciprofloxacinloaded liposomes with bacte  cJUL  a1800 v203 a<p>The process of liposome fusion with cellular membrane plays key role in delivering encapsulated drug molecule into the cell. This process becomes very important for molecules having low permeability as they fail to reach the site of action located inside the cell. Ciprofloxacin (CIP), a broad-spectrum BCS class IV antibiotic, has poor permeability. In the present work, CIP-loaded liposomes were prepared using solvent evaporation method and optimized by 3(2) factorial design approach. The optimized batch of CIP-loaded liposomes was characterized for size, entrapment efficiency, zeta potential, FTIR, and microbial susceptibility study on Staphylococcus aureus (gram-positive bacteria) and Escherichia coli (gram-negative bacteria). Confocal microscopy was used to study the fusogenicity process of CIP-loaded liposomes with bacterial cells. Additionally, the kinetics of fusogenicity process was studied using SAXS for the first time. Surprisingly, the rate of fusion of CIP-loaded liposomes with cell wall of S. aureus was twice when compared to the cell wall of E. coli. It is believed that the current work can act as a roadmap in selection of proper excipients while developing formulations which would expedite the fusogenicity and may execute pharmacological activity of poorly penetrable drug molecules at lower dose.</p>10aciprofloxacin10aconfocal microscopy10afusion10aliposomes10aSAXS1 aPatil, Runali1 aTorris, Arun1 aBhat, Suresh1 aPatil, Sharvil  uhttps://library.ncl.res.in/content/mapping-fusogenicity-ciprofloxacin-loaded-liposomes-bacterial-cells02106nas a2200205   4500008004100000022001400041245012300055210006900178260000900247490000600256520135300262653001901615653003501634653001801669653001401687653002101701100002101722700002701743856013001770       2023                        eng d  a2365-654900aUnderstanding critical aspects of liposomal synthesis for designing the next generation targeted drug delivery vehicle0 aUnderstanding critical aspects of liposomal synthesis for design  cOCT 0 v83 a<p>We identified process parameters of the thin film hydration technique and intrinsic factors to synthesize liposomes for drug delivery. The thin film formation step impacted the nature of the lipid layer, and we optimized 240 RPM rotation speed, 700 mm of Hg vacuum pressure, and 2 ml of chloroform as the organic solvent. The hydration step controlled the particle specifications, and we optimized 270 RPM rotation speed, PBS as the hydrating medium, and 1 h hydration time. We obtained a comparatively smaller liposomal population with a lower size distribution just after hydrating the lipid layer that required milder downsizing steps -10 extrusion passes through a single polycarbonate membrane. The intrinsic factors including the concentrations and molar ratio of lipids affected the synthesis steps and the particle specifications. Characterization of liposomes by analytical techniques confirmed the synthesis of a monodisperse population with hydrodynamic diameter&lt;150 nm, moderate stability, spherical morphology, and high thermal and storage stability. This comprehensive study defines the role of every parameter, provides a mechanistic insight into synthesis that is supported by experimental data, and helps tune specific parameters to synthesize liposomes for drug delivery or any application with desired specifications.</p>
10acarrier system10adesigning and characterization10adrug delivery10aliposomes10atargeting moiety1 aMoudgil, Aliesha1 aChaudhari, Bhushan, P.  uhttps://library.ncl.res.in/content/understanding-critical-aspects-liposomal-synthesis-designing-next-generation-targeted-drug