02317nas a2200253 4500008004100000022001400041245010300055210006900158260011700227300001000344490000600354520131300360653002701673653002201700653002101722653003501743653003701778653002701815653003001842100001901872700001901891700002101910856013201931 2012 eng d a0973-626300aAntibacterial properties of linolenic sophorolipid and its chemically esterified methyl ester form0 aAntibacterial properties of linolenic sophorolipid and its chemi aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcAUG a40-450 v73 a

The antibacterial activity of Linolenic SL mixture (containing 80% lactone) and its methyl ester derivative against Gram positive (B. subtilis) and Gram negative (E. coli and P. aeruginosa) bacteria is reported here. Bacterial cultures were treated with increasing concentrations of Linolenic SL mixture and its methyl ester derivative and antibacterial activity was checked at different time-intervals (2, 4 and 6 hrs) using standard dilution micromethod and spread plate method. Decrease in bacterial colonies was observed with increase in concentrations of compounds as well as incubation time but the level of effectiveness varies with the compound and bacteria. The minimum inhibitory concentrations (MIC) of Linolenic SL mixture (LNNSL, containing 80% lactone) against B. subtilis, E. coli and P. aeruginosa were found to be 20, 10 and 10 mu g ml(-1) respectively. The MIC values of methyl ester form (LNNSLME) against B. subtilis, E. coli and P. aeruginosa were determined to be >20, 20 and 20 mu g ml(-1) respectively. The results suggest that Linolenic SL mixture (containing 80% lactone) as compared to its methyl ester derivative showed good antibacterial activity towards both the Gram positive and Gram negative bacteria and were found to be more potent against Gram negative bacteria.

10aantibacterial activity10aBacillus subtilis10aEscherichia coli10aLinolenic sophorolipid mixture10aMinimum inhibitory concentration10aPseudomonas aeruginosa10aSophorolipid methyl ester1 aReetika, Gupta1 aKumar, Uma, S.1 aAsmita, Prabhune uhttps://library.ncl.res.in/content/antibacterial-properties-linolenic-sophorolipid-and-its-chemically-esterified-methyl-ester-002159nas a2200217 4500008004100000022001400041245012100055210006900176260005700245300001200302490000700314520133900321653002001660653002301680653001301703653002701716653003001743100002201773700002101795856012501816 2015 eng d a0959-399300aNew class of bacterial quorum sensing antagonists: glycomonoterpenols synthesized using linalool and alpha terpineol0 aNew class of bacterial quorum sensing antagonists glycomonoterpe a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJUN a841-8490 v313 a

With increasing burden of antibiotic resistant microorganism search for newer drug targets and potent drug molecules is a never ending scenario. Quorum sensing (QS), the phenomenon of bacterial cross-talk, is one such target that has captured the attention of many and has been touted as the future of new age antimicrobials. Quorum sensing has the potential to regulate a plethora of bacterial virulence phenotypes and search of molecules with powerful quorum sensing inhibitory (QSI) capacity are underway. Monoterpene alcohols like linalool and alpha terpineol have been shown to possess antimicrobial and anti-biofilm activity. However in this article we attempt to bring forth a new class of compounds, glycomonoterpenols, derived from monoterpenoids alcohols. These glycomonoterpenols have been synthesized using Candida bombicola ATCC 22214 by feeding the cells with linalool and alpha terpineol respectively as substrates in 10 % glucose, production medium. The advantage of these molecules over their parent compound is their additional surfactant like property, increased solubility and enhanced QSI potential. A variety of gram-negative bacteria capable of elaborating quorum sensing mediated phenotypes have been selected and both these glycoterpenoid derivatives have been shown to possess strong anti-QS activity.

10aAlpha-terpineol10aGlycomonoterpenols10aLinalool10aPseudomonas aeruginosa10aQuorum sensing inhibition1 aMukherji, Ruchira1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/new-class-bacterial-quorum-sensing-antagonists-glycomonoterpenols-synthesized-using-002325nas a2200289 4500008004100000020004100041245008300082210006900165260012400234520125600358653001901614653002001633653003001653653002701683653001601710100001601726700003101742700001901773700002301792700002201815700002901837700001801866700001301884700001501897700001401912856010901926 2016 eng d a978-81-322-2773-1; 978-81-322-2771-700aRole of Rhamnolipid: A biosurfactant in methane gas hydrate formation kinetics0 aRole of Rhamnolipid A biosurfactant in methane gas hydrate forma a7th Floor, Vijaya Building, 17, Barakhamba Rd, New Delhi, 110 001, IndiabSardar Swaran Singh Natl Inst Bio-EnergycFEB3 a

Naturally occurring methane gas hydrate is a vast source of methane gas which is trapped in crystalline ice-like structure present in permafrost regions and under the sea in outer continental margins. It is purposed that total amount of carbon in the form of methane hydrates is almost twice the carbon content in all the fossil fuel reserves put together, and hence these are supposed to be the future potential energy resource. This paper investigates the laboratory investigations on effect of a biosurfactant rhamnolipid on methane hydrate formation kinetics. Rhamnolipid was produced by Pseudomonas aeruginosa strain A11. The presence of P. aeruginosa has been reported in Gulf of Mexico gas hydrate samples. Biosurfactant reduced the surface tension of water from 72 to 36 mN/m with CMC of 70 mg/L. The biosurfactant dose is studied at two different concentrations in the solution at 100 and 1000 ppm. Kinetic of hydrate formation and growth is compared at 0, 100, and 1000 ppm of rhamnolipid showing that rhamnolipid acts as a hydrate promoter at these concentrations. Thus, small dosages of rhamnolipids produced by P. aeruginosa strain A11 must clearly affect the gas hydrate formation kinetics in natural sites (as in Gulf of Mexico).

10aInduction time10amethane hydrate10aPotential energy resource10aPseudomonas aeruginosa10aRhamnolipid1 aArora, Amit1 aCameotra, Swaranjit, Singh1 aKumar, Rajnish1 aSingh, Anil, Kumar1 aKumar, Pushpendra1 aBalomajumder, Chandrajit1 aLaik, Sukumar1 aKumar, S1 aKhanal, SK1 aYadav, YK uhttps://library.ncl.res.in/content/role-rhamnolipid-biosurfactant-methane-gas-hydrate-formation-kinetics01827nas a2200265 4500008004100000022001400041245010900055210006900164260000800233300001200241490000700253520091500260653001801175653001801193653001801211653004701229653002701276653001701303100001901320700002101339700001801360700002201378700002901400856013201429 2018 eng d a0737-393700aNonconventional method for drying of Pseudomonas aeruginosa and its comparison with conventional methods0 aNonconventional method for drying of Pseudomonas aeruginosa and  cMAY a839-8530 v373 a

In this study, preparation of dried cultures of Pseudomonas aeruginosa using nonconventional drying method, namely, low-temperature low-humidity (LTLH) drying was investigated. The effect of carrier materials (whey protein, corn starch, and trehalose) was examined one at a time and also in combinations (to explore the synergistic effect). The results were compared with those obtained using spray drying and freeze drying in terms of cell survival and dry cell powder yield. The powder samples were analyzed also for morphology, flowability, particle size, and moisture content. In LTLH drying, good cell survival was observed along with high powder yield when compared with that in spray drying. Corn starch showed the highest cell survival (91%) and powder yield (94%, w/w) among the carrier materials employed besides resulting in good cell survival (65%) even after a storage period of 6 months.

10acell survival10aEncapsulation10afreeze drying10aLow-temperature low-humidity (LTLH) drying10aPseudomonas aeruginosa10aspray drying1 aUmashankar, K.1 aChandralekha, A.1 aDandavate, T.1 aTavanandi, H., A.1 aRaghavarao, K., S. M. S. uhttps://library.ncl.res.in/content/nonconventional-method-drying-pseudomonas-aeruginosa-and-its-comparison-conventional-methods