01784nas a2200265   4500008004100000022001400041245008000055210006900135260000800204300001000212490000800222520100100230653000601231653000601237653001701243653001801260653001801278653001601296653001801312100001801330700001701348700001901365700002501384856010901409       2018                        eng d  a0960-852400aMixed bacterial culture mediated direct conversion of bio-glycerol to diols0 aMixed bacterial culture mediated direct conversion of bioglycero  cFEB  a86-930 v2503 a<p>Direct and economic transformation of biodiesel derived crude glycerol is gaining more significance. During screening of bacterial cultures Klebsiella pneumoniae and Enterobacter aerogenes were able to convert crude bio-glycerol to 2,3-butanediol (2,3-BDO) and 1,3-propanediol (1,3-PDO), as major compounds, ethanol and acetoin as minor compounds, with a conversion of 69% and 79% respectively. Process optimization could achieve maximum conversion at pH 7.0, 37 degrees C, 30-40 g/L glycerol and 1.5 g of inoculum until 120 h. Mixed cultures led to complete glycerol conversion with optimal yield and productivity. An innovative approach of using crude glycerol for sustained growth and tolerance of bacteria as source of carbon and energy makes this study more significant. In addition to this, a mixed culture concept introduced here is expected to make impact in process economics for industrial scale synthesis for direct transformation of glycerol into C3 and specifically, C4 diols.</p>10a110a210a3-Butanediol10a3-Propanediol10aBioconversion10aBioglycerol10aMixed culture1 aParate, Roopa1 aMane, Rasika1 aDharne, Mahesh1 aRode, Chandrashekhar  uhttps://library.ncl.res.in/content/mixed-bacterial-culture-mediated-direct-conversion-bio-glycerol-diols02891nas a2200241   4500008004100000022001400041245015700055210006900212260000800281300001000289490000800299520204400307653001902351653001902370653001402389653001802403653000902421653001702430100002502447700002302472700002302495856013102518       2019                        eng d  a0048-969700aBiodegradation of mixed polycyclic aromatic hydrocarbons by pure and mixed cultures of biosurfactant producing thermophilic and thermo-tolerant bacteria0 aBiodegradation of mixed polycyclic aromatic hydrocarbons by pure  cAUG  a52-600 v6793 a<p>Applicability of thermophilic and thereto-tolerant microorganisms for biodegradation of polycyclic aromatic hydrocarbons (PAHs) with low water solubility is an interesting strategy for improving the biodegradation efficiency. In this study, we evaluated utility of thermophilic and thermo-tolerant bacteria isolated from Unkeshwar hot spring (India) for biodegradation of four different PAHs. Water samples were enriched in mineral salt medium (MSM) containing a mixture of four PAHs compounds (anthracene: ANT, fluorene: FLU, phenanthrenc: PHE and pyrene: PYR) at 37 degrees C and 50 degrees C. After growth based screening, four potent strains obtained which were identified as Aeribacillus pallidus (UCPS2), Bacillus axarquiensis (UCPD1), Bacillus siamensis (GHP76) and Bacillus subtilis subsp. inaquosorum (U277) based on the 16S rRNA gene sequence analysis. Degradation of mixed PAH compounds was evaluated by pure as well as mixed cultures under shake flask conditions using MSM supplemented with 200 mg/L concentration of PAHs (50 mg/L of each compound) for 15 days at 37 degrees C and 50 degrees C. A relatively higher degradation of ANT (92% - 96%), FLU (83% - 86%), PHE (16% - 54%) and PYR (51% - 71%) was achieved at 50 degrees C by Aeribacillus sp. (UCPS2) and mixed culture. Furthermore, crude oil was used as a substrate to study the degradation of same PAHs using these organisms which also revealed with similar results with the higher degradation at 50 degrees C. Interestingly, PAH-degrading strains were also positive for biosurfactant production. Biosurfactants were identified as the variants of surfactins (lipopeptide biosurfactants) based on analytical tools and phylogenetic analysis of the surfactin genes. Overall, this study has shown that hot spring microbes may have a potential for PAHs degradation and also biosurfactant production at a higher temperature, which could provide a novel perspective for removal of PAHs residues from oil contaminated sites. (C) 2019 Elsevier B.V. All rights reserved.</p>
10aBiodegradation10aBiosurfactants10aCrude oil10aMixed culture10aPAHs10aThermophiles1 aMehetre, Gajanan, T.1 aDastager, Syed, G.1 aDharne, Mahesh, S.  uhttps://library.ncl.res.in/content/biodegradation-mixed-polycyclic-aromatic-hydrocarbons-pure-and-mixed-cultures-biosurfactant