02839nas a2200325 4500008004100000245014800041210006900189260000800258490000700266520174000273653001002013653002202023653001902045653002602064653001402090653002202104653002202126653002702148653001202175653001402187653000802201653001002209653004502219653002702264100002802291700001902319700001902338700002602357856013002383 2017 eng d00aGenetic variability in Indian populations of banana corm weevil [Cosmopolites sordidus (Coleoptera: Curculionidae)] assessed by RAPDs and AFLPs0 aGenetic variability in Indian populations of banana corm weevil  cSEP0 v373 a

Cosmopolites sordidus (Germar), commonly known as banana corm weevil, is an important economic pest in Asia that can cause severe yield loss depending upon the stage at which infestation occurs. In spite of its economic importance, little is known about the population structure of this pest in India. Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) were used to characterize the population genetic structure of C. sordidus collected from five hot spot locations in India. Nineteen RAPD primers and five selective AFLP primer combinations generated 147 and 304 amplification products, respectively. UPGMA dendrograms generated with both marker systems failed to reveal populations clustered based on geographic distance, which was confirmed by the Mantel test, which did not show a strong correlation between genetic distance and geographic distance. Values of indices of genetic identity showed that the populations were closely related. Though the gene flow estimate (Nm) between the populations was 0.469, suggesting restricted gene flow, the populations are not genetically distinct. These observations suggest that the range expansion of this banana pest in India has taken place through transport of infested corms and plant material, resulting in genetically close populations that are geographically distinct. These results provide important information on the population structure of this pest in India, which will aid in designing suitable strategies for its control and management, especially with respect to insecticide resistance.

10aAflps10aArbitrary Primers10aBemisia-tabaci10aCosmopolites Sordidus10adiversity10aGenetic diversity10aGermar Coleoptera10ainsecticide resistance10aMarkers10aMusa Spp.10aPcr10aRapds10aRestricted Gene Flow Natural-populations10aScirpophaga-incertulas1 aYadav, Sweta, Kumari U.1 aSingh, Jyotsna1 aPadmanaban, B.1 aKumar, Lalitha, Sunil uhttps://library.ncl.res.in/content/genetic-variability-indian-populations-banana-corm-weevil-cosmopolites-sordidus-coleoptera02500nas a2200265 4500008004100000022001400041245009200055210006900147260000900216300001200225490000800237520168300245653001801928653001601946653000801962653001201970653001301982653000801995100002402003700001602027700002302043700002302066700002902089856011602118 2019 eng d a0168-170200aDevelopment and evaluation of taxon-specific primers for the selected Caudovirales taxa0 aDevelopment and evaluation of taxonspecific primers for the sele cAPR  a184-1880 v2633 a

The phage taxonomy is primarily based on the morphology derived from Transmission Electron Microscopic (TEM) studies. TEM based characterization is authentic and accepted by scientific community. However, TEM based identification is expensive and time consuming. After the phage isolation, before analysis TEM, a DNA based rapid method could be introduced. The DNA based method could dramatically reduce the number of samples analyzed by TEM and thereby increase the speed and reduce the cost of identification. In the present work, four environmental phage isolates were identified based on TEM studies and genome size. The identification of these four phages was validated using DNA based method. The taxon-specific DNA markers were identified through multiple sequence alignments. The primers were designed at conserved genes (DNA polymerase or integrase) of 4 different phage taxa viz. family Ackermannviridae, genus Jerseyvirus, genus T4virus, and genus P22virus. These primers were evaluated using both in vitro and in silico approach for the amplification of the target taxons. Majority of the primer sets were found to amplify member species of the targeted taxa in vitro. In In silico analysis, six primer sets intended for identification of family Ackermannviridae showed positive amplification of >= 86.7% classified species. Further, the primers targeting the genus Jerseyvirus and T4virus showed the amplification of 53.8% and >= 84.6% species, respectively. The present work is a case study performed to explore the possibility of use of taxon-specific primers for identification and taxonomic studies of newly isolated phages to supplement the TEM.

10aBacteriophage10aDNA markers10aPcr10aPrimers10aTaxonomy10aTEM1 aNewase, Sandeep, K.1 aGupta, Alka1 aDastager, Syed, G.1 aKapadnis, Balu, P.1 aShashidhar, Ravindranath uhttps://library.ncl.res.in/content/development-and-evaluation-taxon-specific-primers-selected-caudovirales-taxa