02112nas a2200289 4500008004100000022001400041245009300055210006900148260009900217300001400316490000700330520111900337653002201456653001701478653001701495653001701512653001301529100002501542700002101567700002201588700001601610700001701626700002501643700002201668700001601690856011601706 2005 eng d a1359-511300aOptimization and scale up of production of alkaline protease from Conidiobolus coronatus0 aOptimization and scale up of production of alkaline protease fro aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcSEP a3152-31580 v403 a

Alkaline protease secreted by Conidiobolus coronatus has been evaluated extensively in tanneries and finds application in pre-tanning operations in leather manufacture. It is important to produce the enzyme in inexpensive and optimized media on large scale for the process to be commercially viable. The present paper describes optimization of fermentation conditions in shake flasks and scale up of production to 100 L in fermentors. The production is complete in 2-3 days comparable to bacterial fermentations. The organism utilized several carbon sources such as starch, sucrose, lactose, glucose and fructose for production. Soyabean meal at an optimum concentration of 2-3% was found to be best inducer. Diammonium hydrogen phosphate, casamino acids and Hi-media peptone gave activities comparable to yeast extract. Preservation and stabilization studies showed that glycerol conferred considerable stabilization at room temperature while ammonium sulphate precipitated enzyme at 0.9 saturation was best with stability up to 2 years even at room temperature. (c) 2005 Elsevier Ltd. All rights reserved.

10aAlkaline Protease10aConidiobolus10aFermentation10aOptimization10ascale up1 aLaxman, Ryali, Seeta1 aSonawane, A., P.1 aMore, Shivaji, V.1 aRao, B., S.1 aRele, M., V.1 aJogdand, Vitthal, V.1 aDeshpande, V., V.1 aRao, M., B. uhttps://library.ncl.res.in/content/optimization-and-scale-production-alkaline-protease-conidiobolus-coronatus-002359nas a2200253 4500008004100000022001400041245008100055210006900136260008800205300001200293490000700305520151800312653001501830653002001845653001701865653001801882100001701900700001501917700001801932700001801950700001501968700001701983856010502000 2005 eng d a1021-949800aStudies on the lactic-fermentation of sugar apple (Annona squamosa L.) puree0 aStudies on the lacticfermentation of sugar apple Annona squamosa a161-2 KUEN YANG STREET, NANKANG, TAIPEI, TAIWANbNATL LABORATORIES FOODS DRUGScDEC a377-3810 v133 a

Sugar apple (Annona squamosa L.) is a fruit tree of economic importance in Taiwan, with the fruit primarily consumed fresh. Overproduction in recent years, coupled with short shelf life and other practical issues, make practical the research into uses for sugar apples in formats other than fresh. This study presents an exploration of lactic-fermented sugar apple products. The product has a unique sweet aroma and a test panel found its texture to be appealing. For this study, sugar apple puree was used as the substrate for fermentation using mixed starters in a ratio 1:1 or 1:1:1. The high total soluble solid nature (20.5 +/- 4.33 degrees Brix) of sugar apple puree as compared to that of mixed fruit juice (lower than 10 Brix) indicates its potential to be used in fermentation. Following fermentation, the properties and effects of different starter inoculations were recorded and discussed. Fermentation achieved a pH value of 3.8 after 60 hr. The performance of fermented product in the DPPH (alpha,alpha-diphenyl-beta- picrylhydrazyl) decreased from 92% to 78% after 48 hr, followed by a stationary state. Fermented sugar apple puree and fresh sugar apple juice blended in a ratio of 2:8 delivered the highest DPPH scavenging efficiency (88%) and iron chelating ability (49%). The relatively high values of these properties offer the potential for sugar apple juice to be further developed as a novel functional food. Such a development would surely help to ease recent overproduction problems.

10aannonaceae10aDPPH scavenging10aFermentation10aLactobacillus1 aTien, Y., Y.1 aNg, C., C.1 aChang, C., C.1 aTseng, W., S.1 aKotwal, S.1 aShyu, Y., T. uhttps://library.ncl.res.in/content/studies-lactic-fermentation-sugar-apple-annona-squamosa-l-puree-002794nas a2200277 4500008004100000022001400041245014800055210006900203260009900272300001400371490000700385520173200392653003002124653002202154653001702176653002202193653002802215653002302243653002002266100002102286700002002307700002202327700001802349700001702367856013202384 2006 eng d a1359-511300aOptimization of fermentation media for exopolysaccharide production from lactobacillus plantarum using artificial intelligence-based techniques0 aOptimization of fermentation media for exopolysaccharide product aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcAUG a1842-18480 v413 a

A Lactobacillus strain was isolated from the fermented Eleusine coracana. This strain was characterized as Lactobacillus plantarum and was found to produce an exopolysaccharide (EPS) in quantitative amounts. The objective of the present paper is to determine optimum media composition and inoculum volume for the stated fermentative production of the EPS. A hybrid methodology comprising the Plackett-Burman (PB) design method, artificial neural networks (ANN) and genetic algorithms (GA) was utilized. Specifically, the PB, ANN and GA forrnalisms were used for identifying influential media components, modeling non-linear process and optimizing the process, respectively. More specifically, the PB method was used to determine those media components, which significantly influence the EPS yield. By ignoring the less influential media components, the dimensionality of the input space of the process model could be reduced significantly. Out of the five media components only three were found influential namely, lactose, casein hydrolysate and triammonium citrate. Next, an ANN-based process model was developed for approximating the non-linear relationship between the fermentation operating variables and the EPS yield. The average % error and correlation coefficient for the developed ANN model were 4.8 and 0.999, respectively. The input parameters of ANN model were subsequently optimized using the GA formalism for obtaining maximum EPS yield in batch fermentation. The optimized media composition has predicted the yield of 7.01 g/l. The GA-optimized solution comprising media composition and inoculum volume was verified experimentally and it comes out be 7.14 g/l. (c) 2006 Elsevier Ltd. All rights reserved.

10aArtificial neural network10aexopolysaccharide10aFermentation10aGenetic algorithm10aLactobacillus plantarum10aMedia optimization10aPlackett-Burman1 aDesai, Kiran, M.1 aAkolkar, S., K.1 aBadhe, Yogesh, P.1 aTambe, S., S.1 aLele, S., S. uhttps://library.ncl.res.in/content/optimization-fermentation-media-exopolysaccharide-production-lactobacillus-plantarum-using-002105nas a2200265 4500008004100000022001400041245009600055210006900151260006900220300001200289490000700301520118700308653002801495653002001523653001701543653001301560653001701573653001301590100002001603700002201623700002601645700002101671700001701692856013001709 2008 eng d a1369-703X00aOptimal xylanase production using Penicilium janthinellum NCIM 1169: a model based approach0 aOptimal xylanase production using Penicilium janthinellum NCIM 1 aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcJUN a348-3560 v403 a

Xylanases are an industrially important class of hydrolytic enzymes that degrade xylans. Production of xylanase from a fungal culture by submerged fermentation and optimization of the operating conditions for maximum activity are the two aims of the present study. Penicillium janthinellum NCIM 1169 with Mandels-Weber medium, sugarcane bagassse (40\#) as a carbon source and beef extract as a nitrogen source were used in the experiments. We did 41 experiments to see the effect of variations in carbon, nitrogen source, pH, and inoculum on xylanase activity. This data was then used to build an input/output model using multiple linear regression, back propagation neural network and lazy learning algorithm. It was found that lazy learning model correlated well in mapping input/output data. This model was then utilized as an objective function in genetic algorithm to find the optimal combination of the operating conditions to get the maximum xylanase activity. It was observed that with carbon source, 1.63%, nitrogen source, 0.16%, pH, 4.1, and inoculum, 5.5%, maximum xylanase activity of 28.98 +/- 1.73 U/ml was achieved. (C) 2008 Elsevier B.V. All rights reserved.

10aArtificial intelligence10aEnzyme activity10aFermentation10aModeling10aOptimization10aXylanase1 aMeshram, Mukesh1 aKulkarni, Abhijit1 aJayaraman, Valadi, K.1 aKulkarni, B., D.1 aLele, S., S. uhttps://library.ncl.res.in/content/optimal-xylanase-production-using-penicilium-janthinellum-ncim-1169-model-based-approach-001541nas a2200229 4500008004100000022001400041245009200055210006900147260007900216300001200295490000800307520072400315653002401039653002101063653001701084653002601101653001601127653001601143100001601159700002101175856011501196 2009 eng d a0273-228900aBrevibacillus sp: a novel thermophilic source for the production of bile salt hydrolase0 aBrevibacillus sp a novel thermophilic source for the production  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcMAY a254-2620 v1573 a

A thermophilic microorganism growing within the temperature range of 40-65 A degrees C (optimum at 55 A degrees C) was isolated from hot water springs near Konkan, Maharashtra, India. Based on 16S rDNA sequence analysis, it was concluded that the isolate belongs to the genus Brevibacillus. The present paper reports the isolation, identification, and standardization of fermentation conditions for the production of enzyme, bile salt hydrolase (EC 3.5.1.24) which is produced intracellularly at high temperatures. This is the first report regarding the production of bile salt hydrolase from a thermophilic source. Optimization of fermentation conditions resulted in a 2.9-fold enhancement in enzyme production.

10aBile salt hydrolase10aBrevibacillus sp10aFermentation10aGlycodeoxycholic acid10aHot springs10aThermophile1 aSridevi, N.1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/brevibacillus-sp-novel-thermophilic-source-production-bile-salt-hydrolase-002349nas a2200217 4500008004100000022001400041245011100055210006900166260008000235300001200315490000700327520154200334653001701876653001001893653002501903653001101928653001701939100002101956700002601977856012802003 2010 eng d a1367-543500aEnhanced production of gibberellin A(4) (GA(4)) by a mutant of Gibberella fujikuroi in wheat gluten medium0 aEnhanced production of gibberellin A4 GA4 by a mutant of Gibbere aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcMAR a297-3060 v373 a

Mutants of Gibberella fujikuroi with different colony characteristics, morphology and pigmentation were generated by exposure to UV radiation. A mutant, Mor-189, was selected based on its short filament length, relatively high gibberellin A(4) (GA(4)) and gibberellin A(3) (GA(3)) production, as well as its lack of pigmentation. Production of GA(4) by Mor-189 was studied using different inorganic and organic nitrogen sources, carbon sources and by maintaining the pH of the fermentation medium using calcium carbonate. Analysis of GA(4) and GA(3) was done by reversed-phase high-performance liquid chromatography and LC-MS. The mutants of G. fujikuroi produced more GA(4) when the pH of the medium was maintained above 5. During shake flask studies, the mutant Mor-189 produced 210 mg l(-1) GA(4) in media containing wheat gluten as the nitrogen source and glucose as the carbon source. Fed-batch fermentation in a 14 l agitated fermenter was performed to evaluate the applicability of the mutant Mor-189 for the production of GA(4). In 7-day fed-batch fermentation, 600 mg l(-1) GA(4) were obtained in the culture filtrate. The concentration of GA(4) and GA(3) combined was 713 mg l(-1), of which GA(4) accounted for 84% of the total gibberellin. These values are substantially higher than those published previously. The present study indicated that, along with maintenance of pH and controlled glucose feeding, the use of wheat gluten as the sole nitrogen source considerably enhances GA(4) production by the mutant Mor-189.

10aFermentation10aGA(4)10aGibberella fujikuroi10aMutant10aWheat gluten1 aLale, Geetanjali1 aGadre, Ramchandra, V. uhttps://library.ncl.res.in/content/enhanced-production-gibberellin-a4-ga4-mutant-gibberella-fujikuroi-wheat-gluten-medium-001733nas a2200277 4500008004100000022001400041245011000055210006900165260007900234300001200313490000800325520075200333653002201085653001701107653001501124653001101139653002401150653001301174100002501187700002201212700002701234700002401261700002401285700002101309856012501330 2011 eng d a0273-228900aIsolation, identification and optimization of a new extracellular lipase producing strain of rhizopus sp.0 aIsolation identification and optimization of a new extracellular a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcAUG a969-9780 v1643 a

A lipolytic mesophilic fungus which produces lipase extracellularly was isolated from soil. Based on ITS1-5.8S-ITS4 region sequences of ribosomal RNA, it was concluded that the isolate JK-1 belongs to genus Rhizopus and clades with Rhizopus oryzae. The present paper reports the screening, isolation, identification, and optimization of fermentation conditions for the production of lipase (EC 3.1.1.3). Culture conditions were optimized, and the highest lipase production was observed in basal medium with corn steep liquor as nitrogen source and glucose as carbon source. Maximum lipase production was observed at 72 h, which is about 870 U/ml. Optimization of fermentation conditions resulted in 16-fold enhancement in enzyme production.

10aCorn steep liquor10aFermentation10aITS region10aLipase10aMedium optimization10aRhizopus1 aKantak, Jayshree, B.1 aBagade, Aditi, V.1 aMahajan, Siddharth, A.1 aPawar, Shrikant, P.1 aShouche, Yogesh, S.1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/isolation-identification-and-optimization-new-extracellular-lipase-producing-strain-002415nas a2200253 4500008004100000022001400041245015200055210006900207260006900276300001100345490000700356520145300363653002201816653001501838653001701853653003101870653001401901653001701915100002601932700002601958700002801984700002302012856012602035 2011 eng d a1369-703X00aStrain improvement and statistical media optimization for enhanced erythritol production with minimal by-products from candida magnoliae mutant R230 aStrain improvement and statistical media optimization for enhanc aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcJUL a92-1000 v553 a

Mutants of Candida magnoliae NCIM 3470 were generated by ultra-violet and chemical mutagenesis to enhance erythritol production. The mutants were screened for higher reductase activity on agar plates containing high concentration of glucose and 2,3,5-triphenyl tetrazolium chloride (TTC). One of the mutants named as R23 gave maximum erythritol production, 60.3 g L(-1), compared to 14 g L(-1) of the parent strain. Glucose and yeast extract were identified as critical medium components which decide the ratio of polyols produced, mainly erythritol, mannitol and glycerol. In order to enhance the production of erythritol and to minimize the production of mannitol and glycerol, a four component-five level-three response central-composite-rotatable-design (CCRD) of response surface methodology (RSM) model was used. The optimum medium composition for erythritol production was found to contain (g L(-1)) glucose 238, yeast extract 9.2, KH(2)PO(4), 5.16 and MgSO(4) 0.23. Moreover, erythritol production was studied in a 10 L fermentor in batch and fed-batch mode using RSM optimized medium. In fed-batch fermentation, 87.8 g L(-1) erythritol was produced with 31.1% yield, without formation of any other polyols. Thus present study involving strain improvement followed by media and process optimization resulted in 6.2-fold increase in erythritol production and 3.4-fold increase in the yield. (C) 2011 Elsevier B.V. All rights reserved.

10aCandida magnoliae10aErythritol10aFermentation10aMetabolite over-production10aModelling10aOptimization1 aSavergave, Laxman, S.1 aGadre, Ramchandra, V.1 aVaidya, Bhalchandra, K.1 aNarayanan, Karthik uhttps://library.ncl.res.in/content/strain-improvement-and-statistical-media-optimization-enhanced-erythritol-production-002420nas a2200241 4500008004100000022001400041245011100055210006900166260005700235300001400292490000700306520155500313653001701868653001101885653002101896653002101917100002101938700002201959700002601981700001702007700002502024856012902049 2015 eng d a1615-759100aProduction of polyunsaturated fatty acids in recombinant lipomyces starkeyi through submerged fermentation0 aProduction of polyunsaturated fatty acids in recombinant lipomyc a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJUL a1407-14140 v383 a

Long chain polyunsaturated fatty acids (LCPUFA) are known to play an important role in human health and nutrition. Considering the limitation of LCPUFA sources, it is necessary to search new avenues for their production. Oleaginous yeasts are an attractive target for harvesting single cell oil, mainly because of the ease of cultivation with cheaper raw material. Lipomyces starkeyi is one such oleaginous yeast, which can accumulate oil to the extent of 60 % of its biomass and where genetic transformation can be achieved. In our earlier work, Delta 15 desaturase gene (AEP37840) from flax was transformed into L. starkeyi. In the present work, we report optimization of medium for the production of omega-3 enriched oil from this transformed yeast. A basic medium containing 20 g/l glucose as a carbon source and 10 g/l yeast extract as a nitrogen source was used during fermentation. At regular time intervals, glucose was fed to maintain high C:N ratio (65:10) during fermentation. Under the most favorable conditions, dry biomass and total lipid content were 18 and 7.29 g/l, respectively. Prior to genetic transformation, L. starkeyi contained 56.03 mg/l DHA along with 71.4 mg/l EPA and 42.2 mg/l ALA. Genetic engineering of this yeast resulted in a strain that produced 1080 mg/l DHA (17.4 %) along with 74.28 mg/l EPA and 126.72 mg/l ALA possibly through modification of PUFA biosynthetic pathway. To the best of our knowledge, this is a first report of DHA enrichment and opens up avenues for LCPUFA production through L. starkeyi.

10aFermentation10aLCPUFA10aLipid production10aOleaginous yeast1 aSalunke, Devyani1 aManglekar, Rupali1 aGadre, Ramchandra, V.1 aNene, Sanjay1 aHarsulkar, Abhay, M. uhttps://library.ncl.res.in/content/production-polyunsaturated-fatty-acids-recombinant-lipomyces-starkeyi-through-submerged-002381nas a2200289 4500008004100000022001400041245008400055210006900139260000900208300001100217490000800228520148700236653001201723653001701735653002701752653002501779653001201804100001801816700002301834700002801857700002101885700001701906700001801923700001901941700002201960856010901982 2022 eng d a0926-669000aCrystalline xylitol production from corncob biomass with oral toxicity analysis0 aCrystalline xylitol production from corncob biomass with oral to cNOV  a1154070 v1873 a

Xylitol, a five-carbon sugar alcohol, has a steady global market and finds application as a natural sugar substitute in various food and confectionery products. Biocatalytic xylitol production, although touted as a greener alternative to conventional chemical catalysis, suffers from certain challenges, the primary being high cost of production. This study demonstrates a process for food-grade xylitol production from corncob biomass with energy reduction through two major process modifications. A non-conventional fermentation strategy was adopted whereby adjusting aeration without agitation, xylitol with high yield (0.86 +/- 0.015 g/g), and productivity (0.74 gL(-1)h(-1)) could be produced by a GRAS Pichia caribbica MTCC 5703 strain. Xylitol was recovered from the broth in the form of crystals using a combination of membrane-based filtration and crystallization. The crystals demonstrated similar to 98 % purity when quantified with H-1 NMR. Oral toxicity analysis of the crystals demonstrated no adverse effect in female Winstar rats (at a loading of 2000 mg/kg body weight of animals). Overall process statistics showed that 0.584 kg of food-grade xylitol crystals could be produced from 3.5 kg of corncob biomass. The two-process modifications during fermentation and xylitol recovery enabled an energy saving of similar to 20.842 kW/kg of crystals, providing tremendous advantages for biorefinery-based large-scale xylitol production from corncob biomass.

10aBiomass10aFermentation10aOral toxicity analysis10aWastewater treatment10axylitol1 aAhuja, Vishal1 aDasgupta, Diptarka1 aKshirsagar, Siddheshwar1 aGhosh, Prasenjit1 aMore, Snehal1 aGupta, Piyush1 aBehera, Babita1 aBhaskar, Thallada uhttps://library.ncl.res.in/content/crystalline-xylitol-production-corncob-biomass-oral-toxicity-analysis02115nas a2200289 4500008004100000022001400041245010000055210006900155260000800224300001000232490000800242520122000250653001201470653001201482653001901494653001701513653002201530653001201552100002301564700002201587700001801609700001701627700002101644700002201665700001501687856012301702 2022 eng d a0960-308500aProcess development for crystalline xylitol production from corncob biomass by Pichia caribbica0 aProcess development for crystalline xylitol production from corn cMAY a45-560 v1333 a

Xylitol, a commodity chemical, is widely used in nutraceutical and pharmaceutical formulations. Microbial xylitol production is a promising alternative to mitigate current industrial practice issues and offers an environment-friendly sustainable conversion route. This study demonstrates a bioprocess for xylitol production from corncob using a mesophilic yeast Pichia caribbica. Corncob was hydrolyzed by dilute acid and steam explosion to recover fermentable xylose and used as the feed for xylitol production. Activated carbon treatment (3% w/v) completely removed the biomass-derived inhibitors furfural and hydroxymethyl furfural from the liquid hydrolysate. The fermenting yeast Pichia caribbica produced 124.1 +/- 0.45 g/L xylitol from the detoxified and concentrated corncob hydrolysate with a high yield of 0.80 +/- 0.02 g/g. The crystallized xylitol with 96.5% purity demonstrated no harmful effects on the cell line used as a control for the in-vitro toxicity studies. This proof of concept can be applied to help scale up for bio-refinery-based large-scale production of xylitol from corncob biomass.(c) 2022 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved.

10aBiomass10aCorncob10aDetoxification10aFermentation10aToxicity analysis10axylitol1 aDasgupta, Diptarka1 aSidana, Arushdeep1 aSarkar, Bipul1 aMore, Snehal1 aGhosh, Debashish1 aBhaskar, Thallada1 aRay, Anjan uhttps://library.ncl.res.in/content/process-development-crystalline-xylitol-production-corncob-biomass-pichia-caribbica01602nas a2200277 4500008004100000022001400041245011400055210006900169260000800238300001100246490000700257520070400264653002300968653001900991653001701010653001701027653001201044100001801056700002801074700002101102700001801123700001601141700001701157700002301174856012701197 2022 eng d a2213-292900aProcess development for detoxification of corncob hydrolysate using activated charcoal for xylitol production0 aProcess development for detoxification of corncob hydrolysate us cFEB a1070970 v103 aThe work describes process development for detoxification of corncob hydrolysate using activated carbon for microbial xylitol production. Activated carbon was used as an adsorbent to completely remove the major fermentation inhibitors obtained during dilute acid and steam explosion of biomass. A xylitol yield of 0.78 g/g was achieved from the detoxified hydrolysate in a 5 L fermenter by Pichia caribbica. The spent carbon was effectively regenerated and used for three consecutive cycles of operation. The successive regeneration and reuse of carbon could reduce the operational cost by similar to 38% and be used as a reference dataset for process scale-up and cost-effective xylitol production.10aActivated charcoal10aDetoxification10aFermentation10aRegeneration10axylitol1 aAhuja, Vishal1 aKshirsagar, Siddheshwar1 aGhosh, Prasenjit1 aSarkar, Bipul1 aSutar, Ajit1 aMore, Snehal1 aDasgupta, Diptarka uhttps://library.ncl.res.in/content/process-development-detoxification-corncob-hydrolysate-using-activated-charcoal-xylitol01751nas a2200265 4500008004100000022001400041245008800055210006900143260000800212300000800220490000700228520093200235653002401167653001201191653001701203653002401220653001201244100002301256700001801279700002001297700001701317700002101334700001701355856011301372 2023 eng d a0959-399300aFood-grade xylitol production from corncob biomass with acute oral toxicity studies0 aFoodgrade xylitol production from corncob biomass with acute ora cAPR a1020 v393 a

Xylitol, a sugar substitute, is widely used in various food formulations and finds a steady global market. In this study, xylitol crystals were produced from corncob by fermentation (as an alternative to the chemical catalytic process) by a GRAS yeast Pichia caribbica MTCC 5703 and characterized in detail for their purity and presence of any possible contaminant that may adversely affect mammalian cell growth and proliferation. The acute and chronic oral toxicity trials demonstrated no gross pathological changes with average weekly weight gain in female Wistar rats at high xylitol loading (LD50 > 10,000 mg/kg body weight). The clinical chemistry analysis supported the evidence of no dose-dependent effect by analyzing blood biochemical parameters. The finding suggests the possible application of the crystals (> 98% purity) as a food-grade ingredient for commercial manufacture pending human trials.

10aAcute oral toxicity10aCorncob10aFermentation10aShelf life analysis10axylitol1 aDasgupta, Diptarka1 aAhuja, Vishal1 aSingh, Raghuvir1 aMore, Snehal1 aMudliar, Sandeep1 aKumar, Madan uhttps://library.ncl.res.in/content/food-grade-xylitol-production-corncob-biomass-acute-oral-toxicity-studies02655nas a2200241 4500008004100000022001400041245013500055210006900190260000800259300001200267490000700279520182500286653001502111653001702126653002402143653001302167653001702180653002302197100002202220700002302242700002302265856012502288 2024 eng d a1139-670900aStrain improvement for enhanced erythritol production by Moniliella pollinis Mutant-58 using jaggery as a cost-effective substrate0 aStrain improvement for enhanced erythritol production by Monilie cAPR a581-5960 v273 a

Erythritol has been produced by various microorganisms including Yarrowia, Moniliella, Aureobasidium, and Candida strains. Due to its relatively high price, erythritol sweetener is used lesser than other polyols despite having many advantages. Therefore, in this study, Moniliella pollinis strain was improved for erythritol production by chemical mutagenesis and subsequently screening for cost-effective carbon sources for the enhanced erythritol yield. M. pollinis was subjected to N-methyl N-nitro N-nitroso guanidine (NTG), ethyl methyl sulfonate (EMS), and UV mutagenesis for improved erythritol production. The fmutant strains were evaluated for enhanced erythritol production medium optimization by using different carbon substrates at the shake flask level. To enhance the production of erythritol and statistical media, optimization was carried out using a central composite design (CCD). Among 198 isolated mutants, Mutant-58 strain generated by EMS mutagenesis was selected for further assessment. The Mutant-58 strain showed significant morphological changes as compared to the parent strain. Furthermore, statistically optimized media composition resulted in the higher production of erythritol (91.2 & PLUSMN; 3.4 g/L) with a yield of 40.7 & PLUSMN; 3.4 % in shake flask experiments. The optimized medium composition for erythritol production constitutes (g/L) 225 jaggery, 4.4 yeast extract (YE), 4.4 KH2PO4, 0.31 MgSO4, and pH 5.5. The present study demonstrated strain improvement, media, and process optimization resulting in a 30% increase in the erythritol production in the Mutant-58 as compared to the parent strain. This is also the first instance where jaggery has been used as a cost-effective carbon source alternative to glucose for industrial-scale erythritol production.

10aErythritol10aFermentation10aMoniliella pollinis10aMutation10aOptimization10arenewable resource1 aKhatape, Anil, B.1 aRangaswamy, Vidhya1 aDastager, Syed, G. uhttps://library.ncl.res.in/content/strain-improvement-enhanced-erythritol-production-moniliella-pollinis-mutant-58-using02291nas a2200265 4500008004100000022001400041245012000055210006900175260000900244300000800253490000700261520139700268653000601665653002901671653002801700653001801728653001701746653002601763653002601789100002401815700001801839700002301857700002101880856012401901 2025 eng d a0959-399300aAdvances in metabolic engineering and fermentation for 3-hydroxypropionic acid biosynthesis: a comprehensive review0 aAdvances in metabolic engineering and fermentation for 3hydroxyp cSEP  a3520 v413 a

The grand challenge in biobased Manufacturing Lies in achieving the sustainable, economically competitive conversion of renewable biomass into high-value Chemicals capable of replacing fossil-derived products. Among these, 3-hydroxypropionic acid (3-HP) has emerged as a top-tier target-an exceptionally versatile platform molecule. It finds applications in the synthesis of acrylic acid, 1,3-propanediol, and other derivatives, positioning it as a potential cornerstone for bio-based plastics. This review consolidates the latest breakthroughs in microbial 3-HP production, encompassing advanced strain engineering, pathway rewiring, cofactor optimization, metabolic modeling, and flux balance analysis. We critically examine strategies to overcome inherent metabolic and physiological constraints, including byproduct suppression, redox balancing, and tolerance engineering. Emerging approaches-such as dynamic regulation of metabolic flux, control of cell morphology and density, and integration of co-production pathways-are highlighted for their capacity to boost yields and process robustness. Additionally, we address the fermentation process innovations targeting enhanced productivity, substrate efficiency, minimal nutrient input, and industrially relevant titres. Collectively, these insights Chart a clear path toward the scalable, sustainable biomanufacturer of 3-HP.

10a110a3-hydroxypropionaldehyde10a3-hydroxypropionic acid10a3-Propanediol10aFermentation10aflux balance analysis10aMetabolic engineering1 aJoshi, Harshvardhan1 aIsar, Jasmine1 aRangaswamy, Vidhya1 aRaghunathan, Anu uhttps://library.ncl.res.in/content/advances-metabolic-engineering-and-fermentation-3-hydroxypropionic-acid-biosynthesis