01403nas a2200193   4500008004100000022001400041245005800055210005800113260007300171300001000244490000800254520078000262653001701042653000801059653001701067100002101084700001401105856009001119       2005                        eng d  a0378-109700aPHB synthase from Streptomyces aureofaciens NRRL 22090 aPHB synthase from Streptomyces aureofaciens NRRL 2209  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN  a13-180 v2423 a<p>An similar to4.9 kb Sau3A I genomic DNA fragment from the Streptomyces aureofaciens NRRL 2209 aiding in the biosynthesis of PHB in recombinant Escherichia coli has been sequenced and analysed for phaC gene. The putative phaC(sa) gene of 2 kb is 79.1% GC rich and encodes a 63.5 kDa protein. It expressed under its own promoter and significant PHA synthase activity was detected in the recombinant E coli. This is the first putative PHA synthase gene reported from a Streptomyces sp. with serine as the active nucleophile in the conserved lipase box. The phaC(sa) was found in close proximity to a regulatory gene, which apparently regulated the phaC expression. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.</p>10aPHA synthase10aPHB10aStreptomyces1 aRamachander, TVN1 aRawal, SK  uhttps://library.ncl.res.in/content/phb-synthase-streptomyces-aureofaciens-nrrl-2209-0