02220nas a2200241   4500008004100000022001400041245009500055210006900150260008700219300001400306490000700320520133300327653002701660653002501687653002101712653000901733100003301742700001901775700002401794700001801818700002101836856012101857       2006                        eng d  a0011-389100aMicropropagation of Indian laurel (Calophyllum inophyllum), a source of anti-HIV compounds0 aMicropropagation of Indian laurel Calophyllum inophyllum a sourc  aC V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIAbCURRENT SCIENCE ASSNcMAY  a1393-13970 v903 a<p>An efficient protocol for in vitro micropropagtion of Calophyllum inophyllum (Linn.), an evergreen littoral tree, through multiple shoot formation from seed explants was developed. In vitro germination of the seeds was standardized on Woody Plant Medium (WPM) hormone free and/or supplemented with 6-benzylaminopurine (BAP; 2.22 mu M) and on half or full strength MS medium. Multiple shoot formation was achieved on WPM supplemented with BAP (2.22-44.00 mu M) and thidiazuron (TDZ; 0.91-4.54 mu M) from the decapitated seedling explants. The maximum multiple shoots, 20.9 per explant were induced on TDZ (0.91 mu M) after two subcultures. Elongated shoots of size &gt; 4.0 cm were obtained on all media combinations with an average of 2.2-8.7 per explant. Elongation of the stunted shoots induced on BAP and TDZ was done on half strength WPM without any growth hormones. The elongated shoots on half WPM and/or full strength WPM supplemented with indole-3-butyric acid (2.46-24.60 mu M) alone or in combination with BAP (2.22 mu M) resulted in 52% rooting with 1-5 roots per rooted plant. The micropropagated plants were acclimatized successfully with 77% survival rate after five weeks. These plants were planted in the institute campus for ex situ conservation, where 72% plants are showing good growth and development.</p>10aCalophyllum inophyllum10aex situ conservation10amicropropagation10aseed1 aThengane, Shubhada, Ratnakar1 aBhosle, S., V.1 aDeodhar, Swapna, R.1 aPawar, K., D.1 aKulkarni, D., K.  uhttps://library.ncl.res.in/content/micropropagation-indian-laurel-calophyllum-inophyllum-source-anti-hiv-compounds-0