02042nas a2200229   4500008004100000022001400041245016000055210006900215260009700284300001000381490000800391520111700399653002501516653001701541653001801558653002201576100002601598700002201624700002301646700001601669856012701685       2008                        eng d  a0944-501300aInactivation of polygalacturonase and pectate lyase produced by pH tolerant fungus Fusarium moniliforme NCIM 1276 in a liquid medium and in the host tissue0 aInactivation of polygalacturonase and pectate lyase produced by   aOFFICE JENA, P O BOX 100537, 07705 JENA, GERMANYbELSEVIER GMBH, URBAN & FISCHER VERLAGcJAN  a51-620 v1633 a<p>Fusarium moniliforme NCIM 1276 produced pH dependent an extracellular potygalacturonase (PG) and pectate lyase (PL) at pH 5 and pH 8, respectively. In the extracellular medium about 20.3% PG and 54% of PL protein concentrations were present in the active state at pH 5 and pH 8, respectively, whereas in intracelluarly, more than 86% of both protein contents remained in the active state at all pH tested. We found two possible reasons, end-product inhibition and effect of environmental. pH on conformation of the proteins after their release into the medium. Additionally, in infected tomato and cauliflower plants, the fungus secreted similar proteins which were located near to the epidermal and vascular regions of the hypocotyts. In infected tissues, between 26.9% and to 41.5% of PG and only 0.84% - 13.4% of PL protein concentrations were present in active state. Thus, the medium/cell sap pH and concentrations of substrate/end products seem to play an important role in fungal invasion during plant pathogenesis are discussed with current literature. (c) 2006 Elsevier GmbH. All rights reserved.</p>10aFusarium moniliforme10apathogenesis10apectate lyase10apolygatacturonase1 aNiture, Suryakant, K.1 aKumar, Ameeta, R.1 aParab, Pradeep, B.1 aPant, Aditi  uhttps://library.ncl.res.in/content/inactivation-polygalacturonase-and-pectate-lyase-produced-ph-tolerant-fungus-fusarium-002535nas a2200265   4500008004100000022001400041245013800055210006900193260000800262300001400270490000800284520159300292653003701885653001801922653001701940653002401957653002101981100002902002700002202031700002602053700002402079700001402103700002002117856013202137       2017                        eng d  a0175-759800aPenicillin V acylases from gram-negative bacteria degrade N-acylhomoserine lactones and attenuate virulence in Pseudomonas aeruginosa0 aPenicillin V acylases from gramnegative bacteria degrade Nacylho  cMAR  a2383-23950 v1013 a<p>Virulence pathways in gram-negative pathogenic bacteria are regulated by quorum sensing mechanisms, through the production and sensing of N-acylhomoserine lactone (AHL) signal molecules. Enzymatic degradation of AHLs leading to attenuation of virulence (quorum quenching) could pave the way for the development of new antibacterials. Penicillin V acylases (PVAs) belong to the Ntn hydrolase superfamily, together with AHL acylases. PVAs are exploited widely in the pharmaceutical industry, but their role in the natural physiology of their native microbes is not clearly understood. This report details the characterization of AHL degradation activity by homotetrameric PVAs from two gram-negative plant pathogenic bacteria, Pectobacterium atrosepticum (PaPVA) and Agrobacterium tumefaciens (AtPVA). Both the PVAs exhibited substrate specificity for degrading long-chain AHLs. Exogenous addition of these enzymes into Pseudomonas aeruginosa greatly diminished the production of elastase and pyocyanin and biofilm formation and increased the survival rate in an insect model of acute infection. Subtle structural differences in the PVA active site that regulate specificity for acyl chain length have been characterized, which could reflect the evolution of AHL-degrading acylases in relation to the environment of the bacteria that produce them and also provide strategies for enzyme engineering. The potential for using these enzymes as therapeutic agents in clinical applications and a few ideas about their possible significance in microbial physiology have also been discussed.</p>10aN-acylhomoserine lactone acylase10aNtn hydrolase10apathogenesis10aPenicillin Vacylase10aQuorum quenching1 aSunder, Avinash, Vellore1 aUtari, Putri, Dwi1 aRamasamy, Sureshkumar1 avan Merkerk, Ronald1 aQuax, Wim1 aPundle, Archana  uhttps://library.ncl.res.in/content/penicillin-v-acylases-gram-negative-bacteria-degrade-n-acylhomoserine-lactones-and-attenuate02045nas a2200229   4500008004100000022001400041245006700055210006600122260000800188300001400196490000700210520132400217653002501541653001701566653002601583653002301609653001401632100002201646700002401668700002401692856009901716       2020                        eng d  a0006-308800aInsights of Bipolaris sorokinianasecretome-anin silicoapproach0 aInsights of Bipolaris sorokinianasecretomeanin silicoapproach  cDEC  a2367-23810 v753 a<p>The plant pathogen,Bipolaris sorokiniana(teleomorph:Cochliobolus sativus), is of global concern as it attacks many economically important cereals and grasses. During the infection process, phytopathogenic fungi are known to secrete a variety of proteins collectively known as the secretome, analyzing which can help in deciphering the mechanism of fungal pathogenesis. In this study, we performedin silicosecretome analysis ofC. sativusstrain ND90Pr using established secretome prediction pipeline involving software tools such as SignalP, TargetP, TMHMM, big-PI Fungal Predictor, ProtComp, and WoLF PSORT. Using these software and other prediction criteria, we identified 196 probable secretory proteins from theB. sorokinianaproteome. Characterization of the predicted secretome revealed proteins that may have probable functions in degradation of the plant cell wall, lipids, proteins, and nucleic acids, as well as in pathogenesis and metabolism. Further, the PHI-base analysis identified 38 proteins having a possible role in pathogenicity and virulence. This study helped to predict the composition of the secretome ofB. sorokinianaand extrapolate its role in plant infection and pathogen survival. It may provide clues for developing new control strategies targeting the vital fungal secretory proteins.</p>
10aCochliobolus sativus10apathogenesis10aPhytopathogenic fungi10aSecretory proteins10aVirulence1 aPathak, Gauri, M.1 aGurjar, Gayatri, S.1 aKadoo, Narendra, Y.  uhttps://library.ncl.res.in/content/insights-bipolaris-sorokinianasecretome-anin-silicoapproach02169nas a2200277   4500008004100000022001400041245012000055210006900175260000800244300001200252490000700264520118000271653002601451653002001477653002901497653001701526653003001543653003701573653003001610100002001640700002401660700002701684700002301711700002501734856013201759       2024                        eng d  a0046-899100aSystemic review on fitness and survival of salmonella in dynamic environment and conceivable ways of its mitigation0 aSystemic review on fitness and survival of salmonella in dynamic  cJUN  a267-2860 v643 a<p>
	Gastroenteritis caused by non-typhoidal Salmonella still prevails resulting in several recent outbreaks affecting many people worldwide. The presence of invasive non-typhoidal Salmonella is exemplified by several characteristic symptoms and their severity relies on prominent risk factors. The persistence of this pathogen can be attributed to its broad host range, complex pathogenicity and virulence and adeptness in survival under challenging conditions inside the host. Moreover, a peculiar aid of the ever-changing climatic conditions grants this organism with remarkable potential to survive within the environment. Abusive use of antibiotics for the treatment of gastroenteritis has led to the emergence of multiple drug resistance, making the infections difficult to treat. This review emphasizes the importance of early detection of Salmonella, along with strategies for accomplishing it, as well as exploring alternative treatment approaches. The exceptional characteristics exhibited by Salmonella, like strategies of infection, persistence, and survival parallelly with multiple drug resistance, make this pathogen a prominent concern to human health.</p>
10aAntibiotic resistance10aGastroenteritis10aNon-typhoidal Salmonella10apathogenesis10aPlant-derived metabolites10aSalmonella pathogenicity islands10aType III secretion system1 aSutar, Ajit, A.1 aDashpute, Rohit, S.1 aShinde, Yashodhara, D.1 aMukherjee, Srestha1 aChowdhury, Chiranjit  uhttps://library.ncl.res.in/content/systemic-review-fitness-and-survival-salmonella-dynamic-environment-and-conceivable-ways-its