02115nas a2200205   4500008004100000022001400041245015200055210006900207260007300276300001000349490000700359520127600366653003001642653001701672653002701689653002701716100001701743700001901760856013001779       2008                        eng d  a0167-701200aSimple whole cell based high throughput screening protocol using Mycobacterium bovis BCG for inhibitors against dormant and active tubercle bacilli0 aSimple whole cell based high throughput screening protocol using  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcAPR  a62-680 v733 a<p>This study aimed at developing a whole cell based high throughput screening protocol to identify inhibitors against both active and dormant tubercle bacilli. A respiratory type of nitrate reductase (NarGHJI), which was induced during dormancy, could reflect the viability of dormant bacilli of Mycobacterium bovis BCG in microplate adopted model of in vitro dormancy. Correlation between reduction in viability and nitrate reductase activity was seen clearly when dormant stage inhibitor metronidazole and itaconic anhydride were applied in this in vitro microplate model. Active replicating stage could also be monitored in the same assay by measuring the A(620) of the culture. MIC values of 0.08, 0.075, 0.3 and 3.0 mu g/ml, determined through monitoring A(620) in this assay for rifampin, isoniazid, streptomycin and ethambutol respectively, were well in agreement with previously reported by BACTEC and Bio-Siv assays. S/N ratio and Z' factor for the assay were 8.5 and 0.81 respectively which indicated the robustness of the protocol. Altogether the assay provides an easy, inexpensive, rapid, robust and high content screening too] to search novel antitubercular molecules against both active and dormant bacilli. (C) 2008 Elsevier B.V. All rights reserved.</p>10ahigh throughput screening10atuberculosis10aWayne's dormancy model10awhole cell based assay1 aKhan, Arshad1 aSarkar, Dhiman  uhttps://library.ncl.res.in/content/simple-whole-cell-based-high-throughput-screening-protocol-using-mycobacterium-bovis-bcg-0