02175nas a2200253   4500008004100000022001400041245011500055210006900170260008000239300001400319490000800333520125000341653002001591653002101611653001901632653000901651653002601660653001201686100002501698700001801723700001901741700003001760856013101790       2008                        eng d  a1618-264200aImaging the stomatal physiology of somatic embryo-derived peanut leaves by scanning electrochemical microscopy0 aImaging the stomatal physiology of somatic embryoderived peanut   aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcJUL  a2227-22330 v3913 a<p>The stomatal physiology, chlorophyll distribution and photosynthetic activity of somatic embryo (SE)- and seedling-derived peanut plants grown in vitro (test tube-grown) and extra vitrum (soil-grown) are investigated using scanning electrochemical microscopy (SECM). This SECM imaging is performed in two different feedback modes, corresponding to oxygen evolution and chlorophyll distribution. More specifically, the oxygen evolution profiles of the in vitro leaves indicate important differences in leaf anatomy between the SE- and seedling-derived leaves. On the other hand, the chlorophyll distribution images show individual stomata of size ca. 27 +/- 5 mu m. Further studies on senescing (aged) leaves reveal interesting voltammograms that vary widely over the stomatal complexes and the surrounding tissues, probably due to the release of electroactive metabolites during chlorophyll breakdown when the leaves turn yellow. Thus, the present investigation could open up new opportunities for characterizing botanical systems using electroanalytical techniques. In addition, it could provide further insights into various areas of current relevance, including signal transduction, cell fate/differentiation and developmental biology.</p>10aleaf senescence10aoxygen evolution10aphotosynthesis10aSECM10asomatic embryogenesis10astomata1 aParthasarathy, Meera1 aSingh, Shweta1 aHazra, Sulekha1 aPillai, Vijayamohanan, K.  uhttps://library.ncl.res.in/content/imaging-stomatal-physiology-somatic-embryo-derived-peanut-leaves-scanning-electrochemical-0