02055nas a2200229   4500008004100000022001400041245017100055210006900226260011700295300001000412490000600422520111600428653001901544653002101563653001801584653002101602653002501623100001601648700001801664700002001682856012301702       2008                        eng d  a0973-626300aStudies on optimization of cultural conditions and effect of media constituents on production of intracellular penicillin V acylase from Erwinia aroideae (DSMZ 30186)0 aStudies on optimization of cultural conditions and effect of med  aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcMAY  a26-300 v33 a<p>Penicillin V acylase (PVA) is a pharmaceutically important enzyme. Erwinia aroideae (DSMZ 30186) produced high levels of intracellular penicillin V acylase. Amongst various carbon sources used (2.0 %); fructose, galactose, sucrose and mannitol increased enzyme production up to 579, 544, 534 and 504 IU/g dry weight (DW), respectively, compared to minimal media (263 IU/g DW); however maximum PVA productivity (2796 IU/ L) was achieved using fructose. Ammonium ions escalated the enzyme production to 1266.9 IU/g DW; however sodium glutamate was the best nitrogen source for overall productivity of enzyme (2045 IU/ L). Cornsteep liquor and skim milk were used as supplements; 1.0 % cornsteep liquor and 3.0 % skim milk were optimum for the production of PVA up to 823 and 93 IU/g DW, respectively. Optimum cultural conditions for the production of PVA from E. aroideae were standardized. Erwinia aroideae produced 1543 IU/g DW penicillin V acylase in Erlenmeyer flasks (250 ml) containing 50 ml of minimal medium with 0.3% ammonium sulphate, pH 7. 0 at 28 degrees C and 180 rpm when incubated for 56 hr.</p>10acarbon sources10aErwinia aroideae10aintracellular10anitrogen sources10aPenicillin V acylase1 aAtul, Kumar1 aVivek, Lonkar1 aArchana, Pundle  uhttps://library.ncl.res.in/content/studies-optimization-cultural-conditions-and-effect-media-constituents-production-001925nas a2200229   4500008004100000022001400041245013500055210006900190260007300259300001000332490000700342520105100349653002401400653002101424653001901445653002101464653002501485653002101510100001601531700002001547856012801567       2009                        eng d  a1381-117700aEffect of organic solvents on cell-bound penicillin V acylase activity of erwinia aroideae (DSMZ 30186): a permeabilization effect0 aEffect of organic solvents on cellbound penicillin V acylase act  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAY  a67-710 v573 a<p>Erwinia aroideae (DSMZ 30186) is a potential microbial culture to produce intracellular penicillin V acylase (PVA). The whole cell PVA activity was improved by permeabilization with various organic solvents. The cell-bound PVA activity showed an eightfold increase upon treatment with chloroform (5 mu L/mg(dry) (biomass)) for 10 min and diethyl ether (10 mu L/mg(dry) (biomass)) for 45 min. Hexane, toluene, ethyl acetate and dichloromethane enhanced the enzyme activity up to two-, six-, four- and two-fold, respectively; whereas, PVA activity declined drastically on permeabilization with acetone, pyridine and alcohols. The physicochemical properties of the organic solvents used for permeabilization were correlated with the change in activity. It was found that solvents with high hydrophobicity (log P &gt; 0.68) and lower dielectric constant (&lt; 9) were relatively effective in increasing PVA activity. These results allow systematic selection of suitable solvent for best performance. (c) 2008 Elsevier B.V. All rights reserved.</p>10adielectric constant10aErwinia aroideae10aHydrophobicity10aOrganic solvents10aPenicillin V acylase10aPermeabilization1 aKumar, Atul1 aPundle, Archana  uhttps://library.ncl.res.in/content/effect-organic-solvents-cell-bound-penicillin-v-acylase-activity-erwinia-aroideae-dsmz-0