02245nas a2200265 4500008004100000022001400041245012200055210006900177260008100246300001000327490000700337520126400344653002601608653002601634653002701660653003001687653001101717100001801728700001801746700001801764700003001782700002601812700002001838856012101858 2006 eng d a0266-825400aProduction of lactic acid and fructose from media with cane sugar using mutant of Lactobacillus delbrueckii NCIM 23650 aProduction of lactic acid and fructose from media with cane suga a9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLANDbBLACKWELL PUBLISHINGcJUL a53-570 v433 a

Aims: To examine the potential of Lactobacillus delbrueckii mutant, Uc-3 to produce lactic acid and fructose from sucrose-based media. Methods and Results: The mutant of L. delbrueckii NCIM 2365 was cultivated in shake flask containing hydrolysed cane sugar (sucrose)-based medium. The lactic acid yield and volumetric productivity with hydrolysed cane concentration up to 200 g l(-1) were in the range of 92-97% of the theoretical value and between 2.7 and 3.8 g l(-1) h(-1), respectively. The fructose fraction of the syrup produced was more than 95% when the total initial sugar concentration in the medium was higher (150-200 g l(-1)). There are no unwanted byproducts detected in the fermentation broth. Conclusions: We demonstrated that L. delbrueckii mutant Uc-3 was able to utilize glucose preferentially to produce lactic acid and fructose from hydrolysed cane sugar in batch fermentation process. Significance and Impact of the Study: These findings will be useful in the production of lactic acid and high fructose syrups using media with high concentrations of sucrose-based raw materials. This approach can lead to modification of the traditional fermentation processes to obtain value-added byproducts, attaining better process economics.

10afructose accumulation10ahydrolyzed cane sugar10alactic acid production10aLactobacillus delbrueckii10aMutant1 aPatil, S., S.1 aKadam, S., R.1 aPatil, S., S.1 aBastawade, Kulbhushan, B.1 aKhire, Jayant, Malhar1 aGokhale, D., V. uhttps://library.ncl.res.in/content/production-lactic-acid-and-fructose-media-cane-sugar-using-mutant-lactobacillus-002349nas a2200217 4500008004100000022001400041245011100055210006900166260008000235300001200315490000700327520154200334653001701876653001001893653002501903653001101928653001701939100002101956700002601977856012802003 2010 eng d a1367-543500aEnhanced production of gibberellin A(4) (GA(4)) by a mutant of Gibberella fujikuroi in wheat gluten medium0 aEnhanced production of gibberellin A4 GA4 by a mutant of Gibbere aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcMAR a297-3060 v373 a

Mutants of Gibberella fujikuroi with different colony characteristics, morphology and pigmentation were generated by exposure to UV radiation. A mutant, Mor-189, was selected based on its short filament length, relatively high gibberellin A(4) (GA(4)) and gibberellin A(3) (GA(3)) production, as well as its lack of pigmentation. Production of GA(4) by Mor-189 was studied using different inorganic and organic nitrogen sources, carbon sources and by maintaining the pH of the fermentation medium using calcium carbonate. Analysis of GA(4) and GA(3) was done by reversed-phase high-performance liquid chromatography and LC-MS. The mutants of G. fujikuroi produced more GA(4) when the pH of the medium was maintained above 5. During shake flask studies, the mutant Mor-189 produced 210 mg l(-1) GA(4) in media containing wheat gluten as the nitrogen source and glucose as the carbon source. Fed-batch fermentation in a 14 l agitated fermenter was performed to evaluate the applicability of the mutant Mor-189 for the production of GA(4). In 7-day fed-batch fermentation, 600 mg l(-1) GA(4) were obtained in the culture filtrate. The concentration of GA(4) and GA(3) combined was 713 mg l(-1), of which GA(4) accounted for 84% of the total gibberellin. These values are substantially higher than those published previously. The present study indicated that, along with maintenance of pH and controlled glucose feeding, the use of wheat gluten as the sole nitrogen source considerably enhances GA(4) production by the mutant Mor-189.

10aFermentation10aGA(4)10aGibberella fujikuroi10aMutant10aWheat gluten1 aLale, Geetanjali1 aGadre, Ramchandra, V. uhttps://library.ncl.res.in/content/enhanced-production-gibberellin-a4-ga4-mutant-gibberella-fujikuroi-wheat-gluten-medium-001560nas a2200241 4500008004100000022001400041245007600055210006900131260007400200300001200274490000700286520071800293653002701011653002901038653002501067653001101092653002501103100001801128700002001146700002601166700002001192856010601212 2010 eng d a0141-549200aStrain improvement of lactobacillus lactis for d-lactic acid production0 aStrain improvement of lactobacillus lactis for dlactic acid prod aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcAPR a517-5200 v323 a

Three mutants, isolated by repeated UV mutagenesis of Lactobacillus lactis NCIM 2368, produced increased d-lactic acid concentrations. These mutants were compared with the wild type using 100 g hydrolyzed cane sugar/l in the fermentation medium. One mutant, RM2-24, produced 81 g lactic acid/l which was over three times that of the wild type. The highest d-lactic acid (110 g/l) in batch fermentation was obtained with 150 g cane sugar/l with a 73% lactic acid yield. The mutant utilizes cellobiose efficiently, converting it into d-lactic acid suggesting the presence of cellobiase. Thus, this strain could be used to obtain d-lactic acid from cellulosic materials that are pre-hydrolyzed with cellulase.

10aCellobiose utilization10aD-Lactic acid production10aLactobacillus lactis10aMutant10aSucrose (cane sugar)1 aJoshi, D., S.1 aSinghvi, M., S.1 aKhire, Jayant, Malhar1 aGokhale, D., V. uhttps://library.ncl.res.in/content/strain-improvement-lactobacillus-lactis-d-lactic-acid-production-002517nas a2200241 4500008004100000022001400041245011500055210006900170260000800239300000700247490000700254520170100261653002401962653001401986653003202000653001102032653002902043653001602072100002002088700001602108700002202124856012902146 2025 eng d a2191-085500aAspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production0 aAspergillus terreus variant TB21 wet biomass optimized by insitu cFEB a230 v153 a

The oleaginous fungus, Aspergillus terreus when subjected to random chemical mutagenesis led to isolation of TB21 variant with improved lipid content (78.1%) as compared to wild type (49.8%). The fungal wet biomass grown on sugarcane bagasse hydrolysate (SCBH) was subjected to one-step in-situ (direct) acid transesterification to optimize its conversion to biodiesel using a 2-level factorial statistical design of experiments. The process optimization revealed that wet biomass and methanol were the most significant factors and in a short reaction time period of 5 min with low methanol: wet biomass ratio (10:1) influenced FAME production Statistical optimization studies showed that TB21 exhibited a higher FAME content of 76.5 and 38.1% (w/w) from wet and dry biomass, respectively when compared to wild type (48.1 and 24.5%). FAME productivity (0.55-1 h-1) and a yield (66 gL-1) were achieved when TB21 was grown on SCBH for 120 h at 30 degrees C. The FAME profile from the wet biomass of TB21 grown on SCBH had desirable amounts of saturated (77.7%), monounsaturated (7.2%), and polyunsaturated (2.4%) methyl esters. Physico-chemical properties of TB21-derived biodiesel were determined, namely, density(0.88 g cm-3), kinematic viscosity (4.1 mm s-2), iodine value (96.82), cetane number (55.31), free fatty acid content (0.15%), total acid number (0.3 NaOH mg g-1), meeting international (ASTM D6751, EN 14214) and Indian (IS 15607) standards. Thus, the direct one-pot in situ transesterification reaction using wet biomass of variant TB21 strain showed improved production and quality of biodiesel with potential large scale application using the low-cost substrate (SCBH).

10aAspergillus Terreus10aBiodiesel10aIn situ transesterification10aMutant10aStatistical optimization10aWet biomass1 aBed, Rashmi, K.1 aKumar, Ravi1 aRaviKumar, Ameeta uhttps://library.ncl.res.in/content/aspergillus-terreus-variant-tb21-wet-biomass-optimized-situ-transesterification-biodiesel