01848nas a2200229   4500008004100000022001400041245009800055210006900153260005700222300001200279490000700291520103600298653001701334653002001351653002101371653001901392653001901411653002101430100002501451700001901476856012301495       2010                        eng d  a0931-189000aIn vitro regeneration of semecarpus anacardium L. from axenic seedling-derived nodal explants0 aIn vitro regeneration of semecarpus anacardium L from axenic see  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcAUG  a733-7420 v243 a<p>Semecarpus anacardium (Anacardiaceae), a deciduous forest tree, is a potent source of medicinal compounds. Poor seed viability of this species limits the conventional propagation practice. Proliferation of shoots from axillary meristem was achieved in semisolid WPM medium supplemented with BAP 4.44 mu M and KN 4.64 mu M. Factors including culture vessels, gelling agents and antioxidants were identified and optimized for proliferation and growth of shoots in vitro. Cotton-plugged culture vessels were more favorable. Phytagel 0.2% as gelling agent and activated charcoal 0.2% as antioxidant were superior to other agents and antioxidants tested. All the shoots rooted in half-strength WPM liquid medium with IBA 2.46 mu M. Rooted shoots survived (91%) in the soil-sand 1:1 mixture. Ex vitro rooting of shoots and hardening of plants were achieved in 80% of the explants in the soil-sand mixture. Hardened plants were maintained in a greenhouse. This is the first report on in vitro regeneration of Semecarpus anacardium.</p>10aAntioxidants10aCulture vessels10aEx vitro rooting10aGelling agents10aMedicinal tree10amicropropagation1 aPanda, Bhuban, Mohan1 aHazra, Sulekha  uhttps://library.ncl.res.in/content/vitro-regeneration-semecarpus-anacardium-l-axenic-seedling-derived-nodal-explants-0