02127nas a2200265   4500008004100000022001400041245012200055210006900177260009900246300001200345490000800357520114100365653002501506653001401531653003701545653002701582653000901609100001901618700001701637700002401654700002201678700001601700700002101716856012401737       2015                        eng d  a0960-852400aConversion of dried aspergillus candidus mycelia grown on waste whey to biodiesel by in situ acid transesterification0 aConversion of dried aspergillus candidus mycelia grown on waste   aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcDEC  a502-5070 v1973 a<p>This study reports optimization of the transesterification reaction step on dried biomass of an oleaginous fungus Aspergillus candidus grown on agro-dairy waste, whey. Acid catalyzed transesterification was performed and variables affecting esterification, viz., catalyst methanol and chloroform concentrations, temperature, time, and biomass were investigated. Statistical optimization of the transesterification reaction using Plackett-Burman Design showed biomass to be the predominant factor with a 12.5-fold increase in total FAME from 25.6 to 320 mg. Studies indicate that the transesterification efficiency in terms of conversion is favored by employing lower biomass loadings. A. candidus exhibited FAME profiles containing desirable saturated (30.2%), monounsaturated (31.5%) and polyunsaturated methyl esters (38.3%). The predicted and experimentally determined biodiesel properties (density, kinematic viscosity, iodine value, cetane number, TAN, water content, total and free glycerol) were in accordance with international (ASTM D6751, EN 14214) and national (IS 15607) standards. (C) 2015 Published by Elsevier Ltd.</p>10aAspergillus candidus10aBiodiesel10aIn situ acid transesterification10aPlackett-Burman Design10aWhey1 aKakkad, Hardik1 aKhot, Mahesh1 aZinjarde, Smita, S.1 aRaviKumar, Ameeta1 aKumar, Ravi1 aKulkarni, B., D.  uhttps://library.ncl.res.in/content/conversion-dried-aspergillus-candidus-mycelia-grown-waste-whey-biodiesel-situ-acid-002089nas a2200241   4500008004100000022001400041245015400055210006900209260000800278300000800286490000600294520125200300653000901552653001201561653001201573653002701585653003401612100002301646700001601669700001601685700002201701856012401723       2019                        eng d  a2190-572X00aUse of Plackett-Burman design for enhanced phytase production by Williopsis saturnus NCIM 3298 for applications in animal feed and ethanol production0 aUse of PlackettBurman design for enhanced phytase production by   cJUN  a2370 v93 a<p>Distiller-dried grain solid (DDGS), a co-product of alcohol production, contains cereal grain residues, proteins, and yeast metabolites, which make it suitable in poultry feeding. However, high phytate content of DDGS limits its applicability in poultry feed. In this study, Plackett-Burman design was used to improve cell-bound phytase production by Williopsis saturnus NCIM 3298, and we achieved an enzyme activity of 269IU/g of dry-wet biomass. The effect of this enhanced phytase-displaying yeast strain on hydrolysis of corn phytate and subsequently on ethanol production and DDGS quality was then investigated. Results of saccharification in the presence of phytase showed that reducing sugar content of liquefied mash increased by 11%, which subsequently improved the ethanol production by 18% (w/v) (p&lt;0.01) compared with the control. Notably, phytase treatment decreased the phytate content of corn by 70% (p&lt;0.01) compared with the control, thereby improving the availability of free phosphate in fermentation broth and DDGS. Thus, the results obtained suggest that the addition of W. saturnus NCIM 3298 strain has the potential of providing a new source of phytase that would be useful in the feed and ethanol industries.</p>
10aDDGS10aEthanol10aPhytase10aPlackett-Burman Design10aWilliopsis saturnus NCIM 32981 aPable, Anupama, A.1 aShah, Sarah1 aKumar, Ravi1 aKhire, Jayant, M.  uhttps://library.ncl.res.in/content/use-plackett-burman-design-enhanced-phytase-production-williopsis-saturnus-ncim-3298