02299nas a2200265   4500008004100000022001400041245010800055210006900163260007600232300001200308490000700320520135100327653001501678653002001693653001501713653002301728653002201751100001801773700001701791700002701808700001901835700003001854700002201884856012701906       2011                        eng d  a0260-437X00aBiocompatible gellan gum-reduced gold nanoparticles: cellular uptake and subacute oral toxicity studies0 aBiocompatible gellan gumreduced gold nanoparticles cellular upta  aCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USAbWILEY-BLACKWELLcJUL  a411-4200 v313 a<p>Currently gold nanoparticles are being explored for drug delivery and other biomedical applications; therefore it is necessary to study the fate of such nanoparticles inside the body. The objective of the present study was to investigate the cellular uptake and toxicity of the gold nanoparticles synthesized using a microbial polysaccharide, gellan gum, as a capping and reducing agent. The cellular uptake of gold nanoparticles was studied on mouse embryonic fibroblast cells, NIH3T3 and human glioma cell line, LN-229. The cellular uptake study indicated that the gellan gum-reduced gold nanoparticles were located in cancer cells (LN-229) while no uptake was observed in normal mouse embryonic fibroblast cells (NIH3T3). The toxicity of the gold nanoparticles was evaluated by carrying out subacute 28 day oral toxicity studies in rats. Subacute administration of gum-reduced gold nanoparticles to the rats did not show any hematological or biochemical abnormalities. The weight and normal architecture of various organs did not change compared with control. The current findings, while establishing the specific uptake of nanoparticles into cancerous cells, also demonstrates that the gellan gum-reduced gold nanoparticles are devoid of toxicity in animals following oral administration. Copyright (c) 2010 John Wiley &amp; Sons, Ltd.</p>10acell lines10acellular uptake10aGellan gum10agold nanoparticles10asubacute toxicity1 aDhar, Sheetal1 aMali, Vishal1 aBodhankar, Subhash, L.1 aShiras, Anjali1 aPrasad, Bhagavatula L. V.1 aPokharkar, Varsha  uhttps://library.ncl.res.in/content/biocompatible-gellan-gum-reduced-gold-nanoparticles-cellular-uptake-and-subacute-oral-001320nas a2200205   4500008004100000022001400041245012500055210006900180260000900249300001400258490000700272520056800279653001800847653003000865653002000895653002300915100002300938700002400961856012900985       2017                        eng d  a1860-717900a(R-X-R)(4)-Motif peptides containing conformationally constrained cyclohexane-derived spacers: effect on cellular uptake0 aRXR4Motif peptides containing conformationally constrained cyclo  cNOV   a1743-17470 v123 a<p>Arginine-rich peptides having the (R-X-R) n motif are among the most effective cell-penetrating peptides (CPPs). Herein we report a several-fold increase in the efficacy of such CPPs if the linear flexible spacer (-X-) in the (R-X-R) motif is replaced by constrained cyclic 1,4-substituted-cyclohexane-derived spacers. Internalization of these oligomers in mammalian cell lines was found to be an energy-dependent process. Incorporation of these constrained, non-proteinogenic amino acid spacers in the CPPs is shown to enhance their proteolytic stability.</p>10a(R-X-R)-motif10acell-penetrating peptides10acellular uptake10aprotease stability1 aBhosle, Govind, S.1 aFernandes, Moneesha  uhttps://library.ncl.res.in/content/r-x-r4-motif-peptides-containing-conformationally-constrained-cyclohexane-derived-spacers