02415nas a2200253   4500008004100000022001400041245015200055210006900207260006900276300001100345490000700356520145300363653002201816653001501838653001701853653003101870653001401901653001701915100002601932700002601958700002801984700002302012856012602035       2011                        eng d  a1369-703X00aStrain improvement and statistical media optimization for enhanced erythritol production with minimal by-products from candida magnoliae mutant R230 aStrain improvement and statistical media optimization for enhanc  aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcJUL  a92-1000 v553 a<p>Mutants of Candida magnoliae NCIM 3470 were generated by ultra-violet and chemical mutagenesis to enhance erythritol production. The mutants were screened for higher reductase activity on agar plates containing high concentration of glucose and 2,3,5-triphenyl tetrazolium chloride (TTC). One of the mutants named as R23 gave maximum erythritol production, 60.3 g L(-1), compared to 14 g L(-1) of the parent strain. Glucose and yeast extract were identified as critical medium components which decide the ratio of polyols produced, mainly erythritol, mannitol and glycerol. In order to enhance the production of erythritol and to minimize the production of mannitol and glycerol, a four component-five level-three response central-composite-rotatable-design (CCRD) of response surface methodology (RSM) model was used. The optimum medium composition for erythritol production was found to contain (g L(-1)) glucose 238, yeast extract 9.2, KH(2)PO(4), 5.16 and MgSO(4) 0.23. Moreover, erythritol production was studied in a 10 L fermentor in batch and fed-batch mode using RSM optimized medium. In fed-batch fermentation, 87.8 g L(-1) erythritol was produced with 31.1% yield, without formation of any other polyols. Thus present study involving strain improvement followed by media and process optimization resulted in 6.2-fold increase in erythritol production and 3.4-fold increase in the yield. (C) 2011 Elsevier B.V. All rights reserved.</p>10aCandida magnoliae10aErythritol10aFermentation10aMetabolite over-production10aModelling10aOptimization1 aSavergave, Laxman, S.1 aGadre, Ramchandra, V.1 aVaidya, Bhalchandra, K.1 aNarayanan, Karthik  uhttps://library.ncl.res.in/content/strain-improvement-and-statistical-media-optimization-enhanced-erythritol-production-001669nas a2200253   4500008004100000245009700041210006900138260000800207300000900215490000700224520088900231653000901120653001501129653001301144653002301157653000701180653001101187653000601198100002101204700002501225700002001250700002101270856012401291       2022                        eng d00aC-O hydrogenolysis of C3-C4 polyols selectively to terminal diols over Pt/W/SBA-15 catalysts0 aCO hydrogenolysis of C3C4 polyols selectively to terminal diols   cSEP  a10700 v123 a<p>
	Pt/W/SBA-15 catalysts (with Pt-loading = 0.5-4 wt% and W-loading = 1 wt%) prepared by the sequential impregnation method were evaluated for selective C-O cleavage of erythritol and glycerol in an aqueous medium. The Pt and W particles dispersed on SBA-15 approached close proximity at higher Pt loadings and afforded synergistic enhancement in C-O hydrogenolysis activity/selectivity. 1,4-Butanediol yields of 30.9% (at 190 degrees C, 50 bar H-2 and 24 h) and 1,3-propanediol yields of 34.4% (at 190 degrees C, 50 bar H-2 and 12 h of reaction) were obtained over these catalysts. Pt nanoparticles (facilitating dissociative H-2 adsorption and spillover) and W (present as acidic oligomeric WOx species; activating and coordinating the polyol via 1 degrees-OH group) worked in tandem for the selective hydrogenolysis of polyols yielding terminal diols of industrial demand.</p>
10adiol10aErythritol10aGlycerol10aHydrodeoxygenation10aPt10aSBA-1510aW1 aBhowmik, Susmita1 aEnjamuri, Nagasuresh1 aMarimuthu, Banu1 aDarbha, Srinivas  uhttps://library.ncl.res.in/content/c-o-hydrogenolysis-c3-c4-polyols-selectively-terminal-diols-over-ptwsba-15-catalysts01594nas a2200241   4500008004100000022001400041245005500055210005500110260000800165300001200173490000800185520088100193653001501074653002401089653003301113653002301146653001001169653002101179100002201200700002301222700002301245856008401268       2022                        eng d  a0378-109700aOverview of erythritol production by yeast strains0 aOverview of erythritol production by yeast strains  cNOV  afnac1070 v3693 a<p>
	Erythritol is a 4-carbon polyol produced with the aid of microbes in presence of hyper-osmotic stress. It is the most effective sugar alcohol that is produced predominantly by fermentation. In comparison to various polyols, it has many precise functions and is used as a flavor enhancer, sequestrant, humectant, nutritive sweetener, stabilizer, formulation aid, thickener, and a texturizer. Erythritol production is a common trait in a number of the yeast genera viz., Trigonopsis, Candida, Pichia, Moniliella, Yarrowia, Pseudozyma, Trichosporonoides, Aureobasidium, and Trichoderma. Extensive work has been carried out on the biological production of erythritol through Yarrowia, Moniliella, Candida, and other yeast strains, and numerous strategies used to improve erythritol productivity through mutagenesis and genetic engineering are discussed in this review.</p>
10aErythritol10aerythrose reductase10ahyperosmotic stress response10ametabolic pathways10aYeast10ayeast expression1 aKhatape, Anil, B.1 aDastager, Syed, G.1 aRangaswamy, Vidhya  uhttps://library.ncl.res.in/content/overview-erythritol-production-yeast-strains01904nas a2200277   4500008004100000022001400041245011700055210006900172260000900241300001100250490000800261520097300269653002301242653001501265653001901280653001201299653002901311653001801340653003901358100002101397700002101418700001901439700002001458700002101478856012701499       2023                        eng d  a0926-860X00aPromoting effect of titanium on C-O hydrogenolysis of erythritol to 1,4-butanediol over Pt/W/Ti-SBA-15 catalysts0 aPromoting effect of titanium on CO hydrogenolysis of erythritol   cSEP   a1194250 v6663 a<p>
	The C-O hydrogenolysis of erythritol was investigated using Pt/W/Ti-SBA-15 catalysts (4 wt% Pt, 1 wt% W and Si/Ti molar ratio = 50, 33, 20 and 10). Ti-incorporation enhanced the hydrogenolysis activity and the yield of 1,4-butanediol (1,4-BDO). A catalyst with Si/Ti = 20 afforded erythritol conversion of 94 mol% with 1,4-BDO yield of 32.6 mol% and total BDOs yield of 51.7 mol% at 190 degrees C, 50 bar H2 and 12 h. For the catalyst with no titanium (Pt/W/SBA-15), a double the time was required to achieve such yield. There observed electronic connectivity amongst Ti, Pt and W. For the catalyst with Si/Ti = 20, a greater amount of interfacial Pt-O-W(Ti) sites with Pt in + 2 oxidation state was present. The enhanced catalytic performance of these catalysts was corresponded to dispersed Pt (that facilitate hydrogen activation and spillover) and acidic interfacial Pt-O-W sites (that promote the adsorption and hydrogenolysis of erythritol to diols).</p>
10aBiomass conversion10aErythritol10aHydrogenolysis10aPolyols10aPromotional effect of Ti10aTerminal diol10aTi-SBA-15-supported Pt/W catalysts1 aBhowmik, Susmita1 aAkula, Venugopal1 aSethia, Govind1 aMarimuthu, Banu1 aDarbha, Srinivas  uhttps://library.ncl.res.in/content/promoting-effect-titanium-c-o-hydrogenolysis-erythritol-14-butanediol-over-ptwti-sba-1502655nas a2200241   4500008004100000022001400041245013500055210006900190260000800259300001200267490000700279520182500286653001502111653001702126653002402143653001302167653001702180653002302197100002202220700002302242700002302265856012502288       2024                        eng d  a1139-670900aStrain improvement for enhanced erythritol production by Moniliella pollinis Mutant-58 using jaggery as a cost-effective substrate0 aStrain improvement for enhanced erythritol production by Monilie  cAPR  a581-5960 v273 a<p>
	Erythritol has been produced by various microorganisms including Yarrowia, Moniliella, Aureobasidium, and Candida strains. Due to its relatively high price, erythritol sweetener is used lesser than other polyols despite having many advantages. Therefore, in this study, Moniliella pollinis strain was improved for erythritol production by chemical mutagenesis and subsequently screening for cost-effective carbon sources for the enhanced erythritol yield. M. pollinis was subjected to N-methyl N-nitro N-nitroso guanidine (NTG), ethyl methyl sulfonate (EMS), and UV mutagenesis for improved erythritol production. The fmutant strains were evaluated for enhanced erythritol production medium optimization by using different carbon substrates at the shake flask level. To enhance the production of erythritol and statistical media, optimization was carried out using a central composite design (CCD). Among 198 isolated mutants, Mutant-58 strain generated by EMS mutagenesis was selected for further assessment. The Mutant-58 strain showed significant morphological changes as compared to the parent strain. Furthermore, statistically optimized media composition resulted in the higher production of erythritol (91.2 &amp; PLUSMN; 3.4 g/L) with a yield of 40.7 &amp; PLUSMN; 3.4 % in shake flask experiments. The optimized medium composition for erythritol production constitutes (g/L) 225 jaggery, 4.4 yeast extract (YE), 4.4 KH2PO4, 0.31 MgSO4, and pH 5.5. The present study demonstrated strain improvement, media, and process optimization resulting in a 30% increase in the erythritol production in the Mutant-58 as compared to the parent strain. This is also the first instance where jaggery has been used as a cost-effective carbon source alternative to glucose for industrial-scale erythritol production.</p>
10aErythritol10aFermentation10aMoniliella pollinis10aMutation10aOptimization10arenewable resource1 aKhatape, Anil, B.1 aRangaswamy, Vidhya1 aDastager, Syed, G.  uhttps://library.ncl.res.in/content/strain-improvement-enhanced-erythritol-production-moniliella-pollinis-mutant-58-using