01689nas a2200277 4500008004100000022001400041245016700055210006900222260014100291300001400432490000700446520061700453653001501070653002101085653002001106653001001126653001801136100001801154700001301172700001501185700001901200700002101219700001701240700002601257856012801283 2009 eng d a1017-782500aProduction, purification, and characterization of taxol and 10-DABIII from a new endophytic fungus gliocladium sp isolated from the Indian yew tree, taxus baccata0 aProduction purification and characterization of taxol and 10DABI aKOREA SCI TECHNOL CENTER \#507, 635-4 YEOGSAM-DONG, KANGNAM-GU, SEOUL 135-703, SOUTH KOREAbKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGYcNOV a1342-13470 v193 a

We have isolated endophytic fungi from the Indian yew tree, Taxus baccata, and then screened for taxol production. Out of the 40 fungal cultures screened, one fungus Gliocladium sp. was found to produce taxol and 10-DABIII (10-deacetyl baccatin III). These compounds were purified by TLC and HPLC and characterized using UV-spectroscopy, ESI-MS, MS/MS, and proton NMR. One liter of Gliocladium sp. culture yielded 10 mu g of taxol and 65 mu g of 10-DABIII. The purified taxol from the fungus showed cytotoxicity towards cancer lines HL-60 (leukemia), A431 (epidermal carcinoma), and MCF-7 (breast cancer).

10a10 DAB III10aEndophytic fungi10aGliocladium sp.10aTaxol10aTaxus baccata1 aSreekanth, D.1 aSyed, A.1 aSarkar, S.1 aSarkar, Dhiman1 aSanthakumari, B.1 aAhmad, Absar1 aKhan, Mohammad, Islam uhttps://library.ncl.res.in/content/production-purification-and-characterization-taxol-and-10-dabiii-new-endophytic-fungus-002536nas a2200241 4500008004100000022001400041245012700055210006900182260007400251300001200325490000800337520161800345653002301963653002701986653002102013653001502034653002402049100002102073700002002094700002402114700003302138856012302171 2011 eng d a0167-685700aInfluence of endophytic fungal elicitation on production of inophyllum in suspension cultures of Calophyllum inophyllum L.0 aInfluence of endophytic fungal elicitation on production of inop aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcAUG a345-3520 v1063 a

The influence of dried cell powder and culture filtrates of endophytic fungi on production of inophyllum in cell suspension cultures of leaf- and stem-derived callus of Calophyllum inophyllum was investigated. Two fungi, Nigrospora sphaerica and Phoma spp., endophytic to C. inophyllum, were isolated from leaf tissues, and were identified by both 18S rRNA gene amplification and sequencing. Elicitation of suspension cultures of both callus types of C. inophyllum with dried cell powder and culture filtrates of both fungi consistently elicited production of inophyllum A, B, C, and P. In comparison to stem-derived callus, suspension cultures of leaf-derived callus enhanced production of most inophyllum. Of the four inophyllum studied, the highest production of inophyllum A, C, and P was achieved in elicited suspension cultures of leaf-derived callus. Suspension cultures of stem-derived callus enhanced production only of inophyllum B. When suspension cultures of leaf-derived callus were elicited with 40 mg dried cell powder of Phoma spp., a level of 751-fold (6.84 mg/100 g elicited biomass) of inophyllum A was produced, compared to control. Whereas, a level of 414-fold (6.22 mg/100 g elicited biomass) of inophyllum B was produced when suspension cultures of stem-derived callus were elicited with 20 mg dried cell powder of N. sphaerica. When compared to control, a 10% culture filtrate of N. sphaerica in suspension cultures of leaf-derived callus elicited inophyllum C and P production by 928-fold (7.43 mg/100 g elicited biomass) and 750-fold (1.5 mg/100 g elicited biomass), respectively.

10aBiotic elicitation10aCalophyllum inophyllum10aEndophytic fungi10aInophyllum10aSuspension cultures1 aPawar, Kiran, D.1 aYadav, Amit, V.1 aShouche, Yogesh, S.1 aThengane, Shubhada, Ratnakar uhttps://library.ncl.res.in/content/influence-endophytic-fungal-elicitation-production-inophyllum-suspension-cultures-001935nas a2200253 4500008004100000022001400041245008900055210006900144260000800213300001000221490000700231520111400238653001501352653001501367653002101382653002701403653002301430653001701453100002601470700002301496700002401519700002001543856011801563 2023 eng d a0095-362800aGenomic determinants of entomopathogenic fungi and their involvement in pathogenesis0 aGenomic determinants of entomopathogenic fungi and their involve cJAN a49-600 v853 a

Entomopathogenic fungi offer an effective and eco-friendly alternative to curb insect populations in biocontrol strategy. The evolutionary history of selected entomopathogenic fungi indicates their ancestral relationship with plant endophytes. During this host shifting, entomopathogenic fungi must have acquired multiple mechanisms, including a combination of various biomolecules that make them distinguishable from other fungi. In this review, we focus on understanding various biochemical and molecular mechanisms involved in entomopathogenesis. In particular, we attempt to explain the indispensable role of enlarged gene families of various virulent factors, viz. chitinases, proteases, lipases, specialized metabolites, and cytochrome P450, in entomopathogenesis. Our analysis suggests that entomopathogenic fungi recruit a different set of gene products during the progression of pathogenesis. Knowledge of these bio-molecular interactions between fungi and insect hosts will allow researchers to execute pointed efforts towards the development of improved entomopathogenic fungal strains.

10aBiocontrol10aChitinases10aEndophytic fungi10aEntomopathogenic fungi10aHydrolytic enzymes10aInsect pests1 aVidhate, Ravindra, P.1 aDawkar, Vishal, V.1 aPunekar, Sachin, A.1 aGiri, Ashok, P. uhttps://library.ncl.res.in/content/genomic-determinants-entomopathogenic-fungi-and-their-involvement-pathogenesis02957nas a2200277 4500008004100000022001400041245008300055210006900138260000900207300000800216490000700224520205000231653001402281653003102295653002402326653002302350653002102373653002002394653002902414653003002443653002802473100002102501700002602522700002102548856011002569 2026 eng d a0959-399300aEndophytic entomopathogenic fungi: the next frontier in mycological biocontrol0 aEndophytic entomopathogenic fungi the next frontier in mycologic cJUN  a3330 v423 a

Entomopathogenic fungi (EPF) are eco-friendly alternatives to chemical pesticides. However, high costs, the instability of the formulations, sensitivity to environmental factors and variability in virulence limit adoption by farmers. We argue that these problems can be overcome by using EPF strains established as endophytes. The ability of endophytic entomopathogenic fungi (EEPF) to modulate the release of volatiles to attract the predators of insect pests positions them as dual-purpose biocontrol agents in agriculture. As endophytes, these fungi are persistent, lowering the costs for pest control. Endophytic entomopathogenic fungi can also promote plant growth and improve tolerance to abiotic stress. Research on the molecular mechanisms underlying plant tissue colonization by EEPF, their persistence in plants and virulence towards insects suggests that EEPF acquire virulence factors and metabolic versatility through horizontal gene transfer from plants. Therefore, establishing and maintaining EPF as endophytes within plants may compensate for the loss of virulence associated with repeated in vitro subculturing of EPF on artificial media. However, despite these potential advantages of EEPF, challenges still remain, such as variability in endophytic colonization under field conditions, host specificity, ecological risks, and scalability. This review critically evaluates these limitations, focusing on well-studied genera, such as Metarhizium, Beauveria, and Lecanicillium, and outlines future directions for improving the reliability of the application of EEPF. By integrating ecological, molecular, and applied perspectives, we provide a comprehensive and updated framework that positions EEPF as next-generation biocontrol agents in sustainable agriculture.Graphical AbstractEndophytic entomopathogenic fungi protect plants by infecting insect pests and emitting volatiles to attract the natural predators of insect pests. They also promote plant growth by improving the nitrogen fixation and nutrient cycling

10a10aBeauveria10aMetarhizium10aBiological control10aEndophytic fungi10aEntomopathogens10ahorizontal gene transfer10aPlant-fungus interactions10aSustainable agriculture1 aPatil, Karan, S.1 aGathalkar, Ganesh, B.1 aPathan, Ejaj, K. uhttps://library.ncl.res.in/content/endophytic-entomopathogenic-fungi-next-frontier-mycological-biocontrol