02093nas a2200217 4500008004100000022001400041245012400055210006900179260008000248300001400328490000700342520122700349653002401576653002701600653002201627653001701649653002301666100002101689700003301710856013201743 2011 eng d a0137-588100aInfluence of abiotic elicitation on production of dipyranocoumarins in suspension cultures of calophyllum inophyllum L.0 aInfluence of abiotic elicitation on production of dipyranocoumar aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcNOV a2149-21580 v333 a

Effects of elicitation with heavy metals such as copper, cadmium, chromium (abiotic elicitation) and supplementation of CaCl(2) on production of dipyranocoumarins (inophyllums) in suspension cultures of leaf and stem callus of Calophyllum inophyllum were studied. The optimum timing for elicitor introduction was found to be the 10th day after initiating the suspension cultures. Cadmium as abiotic elicitor in suspension cultures of stem callus was found best to elicit maximum production of inophyllums A, C, and calophyllolide while cadmium in suspension cultures of leaf callus was found best for eliciting maximum production of inophyllums B and P. Inophyllum D was the only dipyranocoumarin whose highest production was achieved when 1.0 mM chromium was used as abiotic elicitor in suspension cultures of stem callus. Out of the three abiotic elicitors used, none could result biomass growth. Only incorporation of CaCl(2) in suspension cultures resulted biomass growth. A maximum of 35.26-fold biomass growth was achieved when suspension cultures of stem callus were incorporated with 2.0 mM CaCl(2). CaCl(2) was noted to have no positive influence on production of most of the dipyranocoumarins under study.

10aAbiotic elicitation10aCalophyllum inophyllum10adipyranocoumarins10aHeavy metals10aSuspension culture1 aPawar, Kiran, D.1 aThengane, Shubhada, Ratnakar uhttps://library.ncl.res.in/content/influence-abiotic-elicitation-production-dipyranocoumarins-suspension-cultures-calophyllum-002110nas a2200253 4500008004100000022001400041245015600055210006900211260008600280300001600366490000700382520118400389653001501573653000801588653000901596653001001605653001701615653002201632100002201654700001801676700002201694700001801716856012201734 2016 eng d a1944-399400aSynthesis and characterization of nanoporous silica SBA-15 diaminocyclohexane and its application in removal of Cu(II) and Ni(II) from aqueous solution0 aSynthesis and characterization of nanoporous silica SBA15 diamin a530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USAbTAYLOR & FRANCIS INCcJUL a15397-154090 v573 a

A new nanoporous 1,2-diaminocyclohexane-functionalized SBA-15 (Dach@SBA-15) silica sorbent was prepared. The structure and physicochemical properties of the material were characterized by elemental analysis, X-ray diffraction, transmission electron microscopy (TEM), scanning electron microscopy, FT-IR spectroscopy, nitrogen adsorption-desorption isotherms and thermogravimetric analysis. TEM image clearly showed the sheet-like structure of Dach@SBA-15. The organic functional groups were successfully grafted onto the SBA-15 surface and the ordering of the support was not affected by the chemical modification. The Dach@SBA-15was used as a sorbent for removal of Cu(II) and Ni(II) from aqueous solution. Experimental parameters like effect of pH, contact time and metal ion concentration were studied and optimized. The adsorption isotherm data fitted well to Langmuir isotherm model and the monolayer adsorption capacity values for Cu(II) was 90.09 and for Ni (II) was 84.03 mg/g at 303 K. The experimental kinetic data fitted very well to the pseudosecond- order model. The Dach@SBA-15 could be used as a sorbent for removal of Cu(II) and Ni(II) from aqueous medium.

10aAdsorption10aBET10aFAAS10aFT-IR10aHeavy metals10aNanoporous silica1 aSreenu, Bhogineni1 aSharma, Priti1 aSeshaiah, Kalluru1 aSingh, A., P. uhttps://library.ncl.res.in/content/synthesis-and-characterization-nanoporous-silica-sba-15-diaminocyclohexane-and-its02947nas a2200241 4500008004100000022001200041245009300053210006900146260000900215300001200224490000800236520215500244653002402399653001902423653001602442653001702458653001302475100002502488700001602513700002602529700002302555856012702578 2018 eng d a302893300aUntapped bacterial diversity and metabolic potential within Unkeshwar hot springs, India0 aUntapped bacterial diversity and metabolic potential within Unke cJUL  a753-7700 v2003 a

Hot springs support diverse and interesting groups of microorganisms adapted to extreme conditions and gaining attention in biotechnological applications. However, due to limitations of cultivation methods, a majority of such extremophiles remain uncultivated and unexplored. The advent of multiple cultivation conditions and specialized culture media could possibly aid to access the unexplored microbial portion of hot springs. In the present study, different media and isolation strategies were applied to isolate hitherto unexplored bacterial taxa in the water samples collected from Unkeshwar hot springs, India. Molecular, phylogenetic and predictive functional characterization of the isolated bacterial population was done using 16S rRNA sequencing coupled with Tax4Fun tools. Furthermore, representative isolates were screened for important enzymes (cellulase, xylanase, amylase, and protease) and heavy metal tolerance (chromium, arsenic) properties. A total of 454 bacterial isolates obtained were mapped into 57 unique bacterial genera and 4 different bacterial phyla. Interestingly, 37 genera not previously isolated from Indian hot springs, were isolated for the first time in the present study. However, most of these genera (23 out of 37) were reported only in metagenomics studies from Indian and global hot springs. Furthermore, around 14 genera not previously cultivated and not detected in metagenomics studies of hot springs are documented here. The metabolic potential was ascertained by determining the abundance of specific genes using in silico based Tax4Fun tool, which identified around 315 metabolic pathways for metabolism of carbohydrates, synthesis of secondary metabolites and degradation of xenobiotic compounds. Bioprospection study revealed that 33 and 25 bacterial genera were positive for enzyme production and resistance to the heavy metals, respectively. The present study revealed the advantages of cultivation methods using a comprehensive multiple isolation approach for exploring untapped and unique bacterial diversity, and also utilities for various biotechnological and environmental applications.

10aBacterial diversity10aBioprospecting10aCultivation10aHeavy metals10aUntapped1 aMehetre, Gajanan, T.1 aShah, Manan1 aDastager, Syed, Gulam1 aDharne, Mahesh, S. uhttps://library.ncl.res.in/content/untapped-bacterial-diversity-and-metabolic-potential-within-unkeshwar-hot-springs-india