02220nas a2200241   4500008004100000022001400041245009500055210006900150260008700219300001400306490000700320520133300327653002701660653002501687653002101712653000901733100003301742700001901775700002401794700001801818700002101836856012101857       2006                        eng d  a0011-389100aMicropropagation of Indian laurel (Calophyllum inophyllum), a source of anti-HIV compounds0 aMicropropagation of Indian laurel Calophyllum inophyllum a sourc  aC V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIAbCURRENT SCIENCE ASSNcMAY  a1393-13970 v903 a<p>An efficient protocol for in vitro micropropagtion of Calophyllum inophyllum (Linn.), an evergreen littoral tree, through multiple shoot formation from seed explants was developed. In vitro germination of the seeds was standardized on Woody Plant Medium (WPM) hormone free and/or supplemented with 6-benzylaminopurine (BAP; 2.22 mu M) and on half or full strength MS medium. Multiple shoot formation was achieved on WPM supplemented with BAP (2.22-44.00 mu M) and thidiazuron (TDZ; 0.91-4.54 mu M) from the decapitated seedling explants. The maximum multiple shoots, 20.9 per explant were induced on TDZ (0.91 mu M) after two subcultures. Elongated shoots of size &gt; 4.0 cm were obtained on all media combinations with an average of 2.2-8.7 per explant. Elongation of the stunted shoots induced on BAP and TDZ was done on half strength WPM without any growth hormones. The elongated shoots on half WPM and/or full strength WPM supplemented with indole-3-butyric acid (2.46-24.60 mu M) alone or in combination with BAP (2.22 mu M) resulted in 52% rooting with 1-5 roots per rooted plant. The micropropagated plants were acclimatized successfully with 77% survival rate after five weeks. These plants were planted in the institute campus for ex situ conservation, where 72% plants are showing good growth and development.</p>10aCalophyllum inophyllum10aex situ conservation10amicropropagation10aseed1 aThengane, Shubhada, Ratnakar1 aBhosle, S., V.1 aDeodhar, Swapna, R.1 aPawar, K., D.1 aKulkarni, D., K.  uhttps://library.ncl.res.in/content/micropropagation-indian-laurel-calophyllum-inophyllum-source-anti-hiv-compounds-001941nas a2200229   4500008004100000022001400041245014000055210006900195260009900264300001200363490000700375520102200382653002701404653002201431653002301453653000901476653002201485653002301507100002101530700003301551856012701584       2009                        eng d  a1359-511300aInfluence of hormones and medium components on expression of dipyranocoumarins in cell suspension cultures of calophyllum inophyllum L.0 aInfluence of hormones and medium components on expression of dip  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcAUG  a916-9220 v443 a<p>Cell suspension cultures were initiated separately from leaf and nodal/internodal calluses for the study of influence of hormones and medium components on biomass growth and expression of dipyranocoumarins. Highest 6.2 times biomass was enhanced in suspension cultures of nodal/internodal callus supplemented with threefold total sulphate. Picloram 8.28 mu M along with BAP 8.88 mu M enhanced 295.05 times inophyllum A in suspension cultures of leaf callus whereas IBA 14.70 mu M along with BAP 4.44 mu M in suspension cultures of leaf callus enhanced 1065 times inophyllum B. IBA 4.90 mu M alone in suspension cultures of nodal/internodal callus enhanced maximum 616 times inophyllum C. Only IBA 9.80 mu M in suspension cultures of leaf callus enhanced 23.22 times inophyllum P. Variation in nitrate and sulphate had maximum positive influence on expression of inophyllums A and C and vitamins had maximum positive influence on expression of inophyllums A, C and B. (C) 2009 Elsevier Ltd. All rights reserved.</p>10aCalophyllum inophyllum10adipyranocoumarins10aExpression pattern10aHPLC10aMedium components10aSuspension culture1 aPawar, Kiran, D.1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/influence-hormones-and-medium-components-expression-dipyranocoumarins-cell-suspension-002008nas a2200253   4500008004100000022001400041245014100055210006900196260007400265300001200339490000800351520105100359653002701410653001501437653002201452653000901474653000901483653003301492653002701525100002101552700002101573700003301594856012701627       2011                        eng d  a0378-269700aAssociation between chemical and genetic variation in calophyllum inophyllum, a medicinally important tree of the Western Ghats of India0 aAssociation between chemical and genetic variation in calophyllu  aSACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIAbSPRINGER WIENcAPR  a257-2650 v2923 a<p>The objective of the present work is to study the chemical variation in Calophyllum inophyllum growing along the Western Ghats of India. Contents of dipyranocoumarins (inophyllums) in C. inophyllum were determined to assess whether they could be used as a taxonomic marker for C. inophyllum. This study also aims to establish inter simple sequence repeat (ISSR) markers that can be used to study genetic variation within the species and explore correlation between ISSR and chemical markers. The contents of dipyranocoumarins were estimated in seeds collected from 20 locations. Leaves from plants at the same 20 locations were assayed for ISSR variation. A dendrogram based on Nei's genetic distance as well as principal component analysis based on dipyranocoumarins and ISSR variation clustered plants from these 20 locations into three groups that indicated close relationship among ISSR, dipyranocoumarins contents, and geographical position (variation) of locations. Based on this study, two locations of elite plants were identified.</p>10aCalophyllum inophyllum10aDendrogram10adipyranocoumarins10aHPLC10aISSR10aPrincipal component analysis10aWestern Ghats of India1 aPawar, Kiran, D.1 aJoshi, Swati, P.1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/association-between-chemical-and-genetic-variation-calophyllum-inophyllum-medicinally-002093nas a2200217   4500008004100000022001400041245012400055210006900179260008000248300001400328490000700342520122700349653002401576653002701600653002201627653001701649653002301666100002101689700003301710856013201743       2011                        eng d  a0137-588100aInfluence of abiotic elicitation on production of dipyranocoumarins in suspension cultures of calophyllum inophyllum L.0 aInfluence of abiotic elicitation on production of dipyranocoumar  aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcNOV  a2149-21580 v333 a<p>Effects of elicitation with heavy metals such as copper, cadmium, chromium (abiotic elicitation) and supplementation of CaCl(2) on production of dipyranocoumarins (inophyllums) in suspension cultures of leaf and stem callus of Calophyllum inophyllum were studied. The optimum timing for elicitor introduction was found to be the 10th day after initiating the suspension cultures. Cadmium as abiotic elicitor in suspension cultures of stem callus was found best to elicit maximum production of inophyllums A, C, and calophyllolide while cadmium in suspension cultures of leaf callus was found best for eliciting maximum production of inophyllums B and P. Inophyllum D was the only dipyranocoumarin whose highest production was achieved when 1.0 mM chromium was used as abiotic elicitor in suspension cultures of stem callus. Out of the three abiotic elicitors used, none could result biomass growth. Only incorporation of CaCl(2) in suspension cultures resulted biomass growth. A maximum of 35.26-fold biomass growth was achieved when suspension cultures of stem callus were incorporated with 2.0 mM CaCl(2). CaCl(2) was noted to have no positive influence on production of most of the dipyranocoumarins under study.</p>10aAbiotic elicitation10aCalophyllum inophyllum10adipyranocoumarins10aHeavy metals10aSuspension culture1 aPawar, Kiran, D.1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/influence-abiotic-elicitation-production-dipyranocoumarins-suspension-cultures-calophyllum-002536nas a2200241   4500008004100000022001400041245012700055210006900182260007400251300001200325490000800337520161800345653002301963653002701986653002102013653001502034653002402049100002102073700002002094700002402114700003302138856012302171       2011                        eng d  a0167-685700aInfluence of endophytic fungal elicitation on production of inophyllum in suspension cultures of Calophyllum inophyllum L.0 aInfluence of endophytic fungal elicitation on production of inop  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcAUG  a345-3520 v1063 a<p>The influence of dried cell powder and culture filtrates of endophytic fungi on production of inophyllum in cell suspension cultures of leaf- and stem-derived callus of Calophyllum inophyllum was investigated. Two fungi, Nigrospora sphaerica and Phoma spp., endophytic to C. inophyllum, were isolated from leaf tissues, and were identified by both 18S rRNA gene amplification and sequencing. Elicitation of suspension cultures of both callus types of C. inophyllum with dried cell powder and culture filtrates of both fungi consistently elicited production of inophyllum A, B, C, and P. In comparison to stem-derived callus, suspension cultures of leaf-derived callus enhanced production of most inophyllum. Of the four inophyllum studied, the highest production of inophyllum A, C, and P was achieved in elicited suspension cultures of leaf-derived callus. Suspension cultures of stem-derived callus enhanced production only of inophyllum B. When suspension cultures of leaf-derived callus were elicited with 40 mg dried cell powder of Phoma spp., a level of 751-fold (6.84 mg/100 g elicited biomass) of inophyllum A was produced, compared to control. Whereas, a level of 414-fold (6.22 mg/100 g elicited biomass) of inophyllum B was produced when suspension cultures of stem-derived callus were elicited with 20 mg dried cell powder of N. sphaerica. When compared to control, a 10% culture filtrate of N. sphaerica in suspension cultures of leaf-derived callus elicited inophyllum C and P production by 928-fold (7.43 mg/100 g elicited biomass) and 750-fold (1.5 mg/100 g elicited biomass), respectively.</p>10aBiotic elicitation10aCalophyllum inophyllum10aEndophytic fungi10aInophyllum10aSuspension cultures1 aPawar, Kiran, D.1 aYadav, Amit, V.1 aShouche, Yogesh, S.1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/influence-endophytic-fungal-elicitation-production-inophyllum-suspension-cultures-0