02220nas a2200241 4500008004100000022001400041245009500055210006900150260008700219300001400306490000700320520133300327653002701660653002501687653002101712653000901733100003301742700001901775700002401794700001801818700002101836856012101857 2006 eng d a0011-389100aMicropropagation of Indian laurel (Calophyllum inophyllum), a source of anti-HIV compounds0 aMicropropagation of Indian laurel Calophyllum inophyllum a sourc aC V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIAbCURRENT SCIENCE ASSNcMAY a1393-13970 v903 a

An efficient protocol for in vitro micropropagtion of Calophyllum inophyllum (Linn.), an evergreen littoral tree, through multiple shoot formation from seed explants was developed. In vitro germination of the seeds was standardized on Woody Plant Medium (WPM) hormone free and/or supplemented with 6-benzylaminopurine (BAP; 2.22 mu M) and on half or full strength MS medium. Multiple shoot formation was achieved on WPM supplemented with BAP (2.22-44.00 mu M) and thidiazuron (TDZ; 0.91-4.54 mu M) from the decapitated seedling explants. The maximum multiple shoots, 20.9 per explant were induced on TDZ (0.91 mu M) after two subcultures. Elongated shoots of size > 4.0 cm were obtained on all media combinations with an average of 2.2-8.7 per explant. Elongation of the stunted shoots induced on BAP and TDZ was done on half strength WPM without any growth hormones. The elongated shoots on half WPM and/or full strength WPM supplemented with indole-3-butyric acid (2.46-24.60 mu M) alone or in combination with BAP (2.22 mu M) resulted in 52% rooting with 1-5 roots per rooted plant. The micropropagated plants were acclimatized successfully with 77% survival rate after five weeks. These plants were planted in the institute campus for ex situ conservation, where 72% plants are showing good growth and development.

10aCalophyllum inophyllum10aex situ conservation10amicropropagation10aseed1 aThengane, Shubhada, Ratnakar1 aBhosle, S., V.1 aDeodhar, Swapna, R.1 aPawar, K., D.1 aKulkarni, D., K. uhttps://library.ncl.res.in/content/micropropagation-indian-laurel-calophyllum-inophyllum-source-anti-hiv-compounds-001871nas a2200217 4500008004100000022001400041245012000055210006900175260013100244300000900375490000700384520101500391653001601406653001401422653001001436653002101446653002201467100002001489700001801509856012601527 2007 eng d a0972-853800aEffect of auxin types on in vitro and ex vitro rooting and acclimatization of grapevine as influenced by substrates0 aEffect of auxin types on in vitro and ex vitro rooting and accli aDIV FRUITS & HORTICULTURAL TECHNOL, INDIAN AGRICULTURAL RESEARCH INST, NEW DELHI, 110 012, INDIAbHORTICULTURAL SOC INDIAcMAR a5-110 v643 a

Transferring micropropagated plantlets from in vitro to in vivo conditions is a critical step because plantlets have under-developed cuticle and stomata which result in wilting due to low humidity, fungal attack and bleaching in high light intensities. The resumption of terminal growth indicates successful establishment which is a change from mixotrophy to autotrophy. The survival of Vitis vinifera cvs. 2A-Clone and Red Globe plantlets rooted in vitro was 86.66 and 83.33%, respectively and the established plants showed a well-defined root system. Plantlets rooted in vivo by auxin pulse were easier to transfer and acclimatize with a survival percentage of 96.66 and 79.92 for 2A-Clone and Red Globe, respectively but the rooting was of fibrous type with few main roots and less number of root hairs. Coco-peat in combination with sand and soil was better for quick acclimatization and establishment (97.5 and 95% survival for 2A-Clone and Red Globe respectively) of the micropropagated plantlets.

10aauxin pulse10acoco-peat10agrape10amicropropagation10aplantlet survival1 aBarreto, M., S.1 aNookaraju, A. uhttps://library.ncl.res.in/content/effect-auxin-types-vitro-and-ex-vitro-rooting-and-acclimatization-grapevine-influenced01492nas a2200289 4500008004100000022001400041245009900055210006900154260013100223300001200354490000700366520045200373653001600825653001400841653001000855653002100865653002200886100001800908700002200926700001700948700002400965700002600989700001801015700002501033700001801058856012601076 2007 eng d a0972-853800aMacroporous beads for lipase immobilization: kinetic resolution of a racemic drug intermediate0 aMacroporous beads for lipase immobilization kinetic resolution o aDIV FRUITS & HORTICULTURAL TECHNOL, INDIAN AGRICULTURAL RESEARCH INST, NEW DELHI, 110 012, INDIAbHORTICULTURAL SOC INDIAcMAR a174-1940 v223 a

{Lipase isolated from Arthrobacter sp. (RRLJ-1, MTCC No. 5125, named ABL), is effective in resolving a wide range of racemic drug intermediates. In this study, ABL was immobilized on a series of synthetic macroporous epoxy copolymers beads with varying pore sizes, surface area and hydrophobicity. Poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads, with 75% crosslink density and 10% of epoxy groups modified with dibutyl amine [

10aauxin pulse10acoco-peat10agrape10amicropropagation10aplantlet survival1 aBhushan, Indu1 aParshad, Rajinder1 aQazi, G., N.1 aIngavle, Ganesh, C.1 aJamalpure, Trupti, M.1 aRajan, C., R.1 aPonrathnam, Surendra1 aGupta, V., K. uhttps://library.ncl.res.in/content/macroporous-beads-lipase-immobilization-kinetic-resolution-racemic-drug-intermediate-002839nas a2200301 4500008004100000020002200041022001400063245007100077210006900148260030100217300001200518520163500530653001602165653001402181653002202195653002102217653001902238653001002257100002002267700001802287700002102305700002402326700002402350700001902374700001902393700002302412856010202435 2008 eng d a978-90-6605-268-0 a0567-757200aVariability among vitis vinifera cultivars to in vitro propagation0 aVariability among vitis vinifera cultivars to in vitro propagati aPo Box 500, 3001 Leuven 1, BelgiumbAgr & Processed Food Prod Export Dev Author; Indian Farmers Fertilizer Cooperat Ltd; Maharashtra Agro Industries Dev Corp Ltd; Indian Council Agr Res; Natl Res Ctr Grapes; Federat Indian Chambers Commerce & Ind; Natl Hort Board; Minist Food Proc IndustriecMAY a127-1393 a

Response of grapevines to tissue culture has been reported to be genotype dependent, hence it becomes imperative to optimize culture conditions for newly developed varieties or cultivars, needing a large scale planting but availability of sufficient planting stock is a constraint. In the present study, conditions for in vitro propagation of six popular table grape cultivars have been standardized. Single node stem segments of cultivars 2A-Clone of Thompson Seedless, Red Globe, Crimson Seedless, Thompson Seedless, Flame Seedless and Italia cultured on ten different basal media showed varied percentages of bud break and morphogenetic responses. The percentage of bud break varied among the six cultivars. Direct rooting at basal ends of single node segments was observed in all the cultivars except in Italia. Woody plant medium induced the highest response and Eriksson medium the least in all the cultivars tested. Rooted nodal segments with shoots in axils could be established into whole plants on potting. Six cultivars showed different optimum concentrations of growth regulators for induction of maximum number of multiple shoots in both primary as well as secondary nodal segments. Shoot proliferation could be enhanced by several fold in the majority of cultivars on culture of initial shoot clumps to glass bottles instead of culture tubes. Six cultivars showed significant differences in optimum requirement of nutrients and growth regulators for shoot elongation, in vitro and ex vitro rooting and survival rate. Tissue culture plants of all the six cultivars could establish successfully in a greenhouse.

10abasal media10aGrapevine10agrowth regulators10amicropropagation10aTissue culture10aVitis1 aBarreto, M., S.1 aNookaraju, A.1 aJoglekar, A., M.1 aKaribasappa, G., S.1 aAgrawal, Dinesh, C.1 aAdsule, P., G.1 aSawant, I., S.1 aShikhamany, S., D. uhttps://library.ncl.res.in/content/variability-among-vitis-vinifera-cultivars-vitro-propagation-001848nas a2200229 4500008004100000022001400041245009800055210006900153260005700222300001200279490000700291520103600298653001701334653002001351653002101371653001901392653001901411653002101430100002501451700001901476856012301495 2010 eng d a0931-189000aIn vitro regeneration of semecarpus anacardium L. from axenic seedling-derived nodal explants0 aIn vitro regeneration of semecarpus anacardium L from axenic see a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcAUG a733-7420 v243 a

Semecarpus anacardium (Anacardiaceae), a deciduous forest tree, is a potent source of medicinal compounds. Poor seed viability of this species limits the conventional propagation practice. Proliferation of shoots from axillary meristem was achieved in semisolid WPM medium supplemented with BAP 4.44 mu M and KN 4.64 mu M. Factors including culture vessels, gelling agents and antioxidants were identified and optimized for proliferation and growth of shoots in vitro. Cotton-plugged culture vessels were more favorable. Phytagel 0.2% as gelling agent and activated charcoal 0.2% as antioxidant were superior to other agents and antioxidants tested. All the shoots rooted in half-strength WPM liquid medium with IBA 2.46 mu M. Rooted shoots survived (91%) in the soil-sand 1:1 mixture. Ex vitro rooting of shoots and hardening of plants were achieved in 80% of the explants in the soil-sand mixture. Hardened plants were maintained in a greenhouse. This is the first report on in vitro regeneration of Semecarpus anacardium.

10aAntioxidants10aCulture vessels10aEx vitro rooting10aGelling agents10aMedicinal tree10amicropropagation1 aPanda, Bhuban, Mohan1 aHazra, Sulekha uhttps://library.ncl.res.in/content/vitro-regeneration-semecarpus-anacardium-l-axenic-seedling-derived-nodal-explants-002211nas a2200205 4500008004100000022001400041245008700055210006900142260009200211300001000303490000800313520144000321653001701761653002101778653002901799653001601828100002501844700001901869856011701888 2012 eng d a1126-350400aMicropropagation of semecarpus anacardium L.: a medicinally important tree species0 aMicropropagation of semecarpus anacardium L a medicinally import a4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLANDbTAYLOR & FRANCIS LTDcOCT a61-680 v1463 a

Semecarpus anacardium L., a deciduous forest tree, is a source of medicinal compounds. Poor seed-viability restricts the conventional propagation. Micropropagation protocol is standardized for this species. Shoot culture-derived nodal explants were cultured in woody plant media supplemented with thidiazuron (TDZ). Shoot differentiation from meristem was limited. Meristems swelled to form meristematic mass in higher concentrations of TDZ. Swelling of meristem was attributed to the proliferation of meristematic cells. Development of shoots from meristematic mass on withdrawal of TDZ in culture medium indicated the inhibitory influence of TDZ on differentiation of buds to form shoots. Harvesting the primary shoot, leads to appearance of additional shoot buds which elongated on repeated transfer of explants in a medium devoid of growth regulator every four weeks. Optimum (17) number of shoots obtained from each meristem in explants pre-cultured in TDZ 2.27 mu M and re-cultured in growth regulator free medium for seven cycles (28 weeks). This confirms the stimulatory influence of TDZ on proliferation of meristem and inhibitory influence on shoot differentiation. All shoots, rooted in the medium with Indole butyric acid 2.46 mu M. Plantlets survived on transfer to sand: soil (1: 1) mixture and acclimatized. This is the first report on micropropagation of S. anacardium from seedling derived nodal buds using TDZ.

10aMeristematic10amicropropagation10aSemecarpus anacardium L.10athidiazuron1 aPanda, Bhuban, Mohan1 aHazra, Sulekha uhttps://library.ncl.res.in/content/micropropagation-semecarpus-anacardium-l-medicinally-important-tree-species-0