01689nas a2200277 4500008004100000022001400041245010500055210006900160260007700229300001200306490000700318520072700325653001201052653002501064653001301089653001501102653001401117100002401131700002701155700002301182700001601205700001801221700002301239700001801262856013101280 2012 eng d a1439-422700aAntagonism of microRNA function in zebrafish embryos by using locked nucleic acid enzymes (LNAzymes)0 aAntagonism of microRNA function in zebrafish embryos by using lo aPO BOX 10 11 61, D-69451 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcMAR a584-5890 v133 a

MicroRNAs (miRNAs) have crucial functions in many cellular processes, such as differentiation, proliferation and apoptosis; aberrant expression of miRNAs has been linked to human diseases, including cancer. Tools that allow specific and efficient knockdown of miRNAs would be of immense importance for exploring miRNA function. Zebrafish serves as an excellent vertebrate model system to understand the functions of miRNAs involved in a variety of biological processes. We designed and employed a strategy based on locked nucleic acid enzymes (LNAzymes) for in vivo knockdown of miRNA in zebrafish embryos. We demonstrate that LNAzyme can efficiently knockdown miRNAs with minimal toxicity to the zebrafish embryos.

10aenzymes10alocked nucleic acids10amicroRNA10amorpholino10azebrafish1 aSuryawanshi, Hemant1 aLalwani, Mukesh, Kumar1 aRamasamy, Soundhar1 aRana, Rajiv1 aScaria, Vinod1 aSivasubbu, Sridhar1 aMaiti, Souvik uhttps://library.ncl.res.in/content/antagonism-microrna-function-zebrafish-embryos-using-locked-nucleic-acid-enzymes-lnazymes-001541nas a2200241 4500008004100000022001400041245013200055210006900187260000800256300001200264490000700276520072300283653001501006653001101021653001301032653001801045653002101063100001701084700002201101700002601123700001801149856013201167 2016 eng d a2159-333700aInhibition of miR-21 by 3 `/5 `-serinyl-capped 2 `-O-Methyl RNA Interspersed with 2 `-O-(2-Amino-3-Methoxypropyl) uridine units0 aInhibition of miR21 by 3 5 serinylcapped 2 OMethyl RNA Intersper cOCT a327-3340 v263 a

miRNAs are highly conserved class of small ncRNAs whose involvement in human pathophysiologies is extensively investigated. MiR-21 is a well established oncogenic miRNA whose deregulation plays a significant role in onset and progression of cancer. The need of novel approaches to downregulate miR-21 is rapidly expanding. Potent inhibition of miR-21 is achieved by chemically modified 2-O-methyl RNA oligonucleotide. The serinol capping at 3 and 5ends and the interspersed 2-O-(R-2-amino-3-methoxypropyl) uridine units enhance the nuclease resistance and efficacy of 2-O-methyl RNA for the inhibition of miR-21. This represents a simple and novel modification for developing oligonucleotide-based therapeutics.

10aantagomirs10acancer10amicroRNA10anoncoding RNA10aoligonucleotides1 aNahar, Smita1 aKotikam, Venubabu1 aKumar, Vaijayanti, A.1 aMaiti, Souvik uhttps://library.ncl.res.in/content/inhibition-mir-21-3-5-serinyl-capped-2-o-methyl-rna-interspersed-2-o-2-amino-3-methoxypropyl02679nas a2200325 4500008004100000022001400041245013000055210006900185260000900254300001400263490000700277520154600284653003601830653001301866653001101879653002701890653002201917653002201939653003301961100002301994700002402017700002202041700002002063700002902083700002502112700003702137700002802174700002402202856012702226 2018 eng d a0022-095700aMiRNA160 is associated with local defense and systemic acquired resistance against Phytophthora infestans infection in potato0 aMiRNA160 is associated with local defense and systemic acquired  cAPR  a2023-20360 v693 a

To combat pathogen infection, plants employ local defenses in infected sites and elicit systemic acquired resistance (SAR) in distant tissues. MicroRNAs have been shown to play a significant role in local defense, but their association with SAR is unknown. In addition, no such studies of the interaction between potato and Phytophthora infestans have been reported. We investigated the role of miR160 in local and SAR responses to P. infestans infection in potato. Expression analysis revealed induced levels of miR160 in both local and systemic leaves of infected wild-type plants. miR160 overexpression and knockdown plants exhibited increased susceptibility to infection, suggesting that miR160 levels equivalent to those of wild-type plants may be necessary for mounting local defense responses. Additionally, miR160 knockdown lines failed to elicit SAR, and grafting assays indicated that miR160 is required in both local and systemic leaves to trigger SAR. Consistently, SAR-associated signals and genes were dysregulated in miR160 knockdown lines. Furthermore, analysis of the expression of defense and auxin pathway genes and direct regulation of StGH3.6, a mediator of salicylic acid-auxin cross-talk, by the miR160 target StARF10 revealed the involvement of miR160 in antagonistic cross-talk between salicylic acid-mediated defense and auxin-mediated growth pathways. Overall, our study demonstrates that miR160 plays a crucial role in local defense and SAR responses during the interaction between potato and P. infestans.

10aAuxin-salicylic acid antagonism10amicroRNA10amiR16010aPhytophthora infestans10aSolanum chacoense10aSolanum tuberosum10asystemic acquired resistance1 aNatarajan, Bhavani1 aKalsi, Harpreet, S.1 aGodbole, Prajakta1 aMalankar, Nilam1 aThiagarayaselvam, Aarthy1 aSiddappa, Sundaresha1 aThulasiram, Hirekodathakallu, V.1 aChakrabarti, Swarup, K.1 aBanerjee, Anjan, K. uhttps://library.ncl.res.in/content/mirna160-associated-local-defense-and-systemic-acquired-resistance-against-phytophthora02587nas a2200241 4500008004100000022001400041245011400055210006900169260000800238300001200246490000800258520175700266653002402023653002402047653002802071653001302099653002402112653002102136653001802157100001902175700002102194856013002215 2023 eng d a1617-461500aIdentification of potential microRNAs regulating metabolic plasticity and cellular phenotypes in glioblastoma0 aIdentification of potential microRNAs regulating metabolic plast cJAN a161-1810 v2983 a

MicroRNAs (miRNAs) play important role in regulating cellular metabolism, and are currently being explored in cancer. As metabolic reprogramming in cancer is a major mediator of phenotypic plasticity, understanding miRNA-regulated metabolism will provide opportunities to identify miRNA targets that can regulate oncogenic phenotypes by taking control of cellular metabolism. In the present work, we studied the effect of differentially expressed miRNAs on metabolism, and associated oncogenic phenotypes in glioblastoma (GBM) using patient-derived data. Networks of differentially expressed miRNAs and metabolic genes were created and analyzed to identify important miRNAs that regulate major metabolism in GBM. Graph network-based approaches like network diffusion, backbone extraction, and different centrality measures were used to analyze these networks for identification of potential miRNA targets. Important metabolic processes and cellular phenotypes were annotated to trace the functional responses associated with these miRNA-regulated metabolic genes and associated phenotype networks. miRNA-regulated metabolic gene subnetworks of cellular phenotypes were extracted, and important miRNAs regulating these phenotypes were identified. The most important outcome of the study is the target miRNA combinations predicted for five different oncogenic phenotypes that can be tested experimentally for miRNA-based therapeutic design in GBM. Strategies implemented in the study can be used to generate testable hypotheses in other cancer types as well, and design context-specific miRNA-based therapy for individual patient. Their usability can be further extended to other gene regulatory networks in cancer and other genetic diseases.

10aCellular phenotypes10aFunctional analysis10aGlioblastoma metabolism10amicroRNA10amiRNA-based therapy10aNetwork analysis10aTranscriptome1 aBhowmick, Rupa1 aSarkar, Ram, Rup uhttps://library.ncl.res.in/content/identification-potential-micrornas-regulating-metabolic-plasticity-and-cellular-phenotypes02558nas a2200253 4500008004100000022001400041245009000055210006900145260000800214300001200222490000700234520173800241653001901979653001301998653001802011653002302029653001602052653002602068100001602094700002502110700002702135700002402162856011802186 2025 eng d a0971-781100aHarnessing microRNAs and their targets for salt stress tolerance in Pandanus odorifer0 aHarnessing microRNAs and their targets for salt stress tolerance cSEP a714-7300 v343 a

Salinity poses a formidable challenge to agriculture, detrimentally impacting plant growth and the yield of several crops globally. Pandanus odorifer (Forssk.) Kuntze is a highly salt-stress resilient plant that grows naturally along the coastal regions of Asia, Southeast Asia, and Polynesia. It is frequently found as a frontier species along the sandy shores receiving constant salt sprays. MicroRNAs (miRNAs) play essential regulatory roles in growth, development, and combating various biotic and abiotic stresses. Thus, characterizing miRNAs and their targets is critical to understanding their roles in salt stress tolerance. Through a rigorous analysis based on homology, secondary structure and signatures of other miRNA genes, we identified 52 conserved nonredundant miRNAs from the P. odorifer transcriptome data. These miRNAs were diverse and belonged to 23 miRNA phylogenetic families. Several miRNAs, including pod-MIR156a, pod-MIR164d, and pod-MIR535d, demonstrated distinct expression patterns in response to salt stress. Their target genes exhibited opposing expression trends, corroborating the intricate miRNA-target relationship. Delving deeper into their influence, we discovered that these miRNAs mainly targeted transcription factors that are known for their pivotal roles in stress responses. We also detected four miRNA-star (miRNA*) sequences (the complementary strands of functional mature miRNAs), which supports the authenticity of the identified miRNAs. This study unveils the intricate regulatory mechanism through which miRNAs may reinforce salt stress tolerance in P. odorifer, providing a promising avenue for enhancing crop resilience to various stresses through miRNA-based strategies.

10aAbiotic stress10amicroRNA10amiRNA targets10aSalinity tolerance10asalt stress10aTranscription factors1 aRashmi, Deo1 aBarvkar, Vitthal, T.1 aNadaf, Altafhusain, B.1 aKadoo, Narendra, Y. uhttps://library.ncl.res.in/content/harnessing-micrornas-and-their-targets-salt-stress-tolerance-pandanus-odorifer