01618nas a2200229 4500008004100000022001400041245006800055210006800123260009900191300001200290490000800302520081600310653004201126653000801168653000901176653001101185653000801196100003201204700002101236700002601257856010501283 2012 eng d a0960-852400aEfficient lipase purification using reverse micellar extraction0 aEfficient lipase purification using reverse micellar extraction aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcMAR a224-2300 v1083 a

Reverse micellar extraction (RME) of enzyme provides an attractive option for conventional method with the potential to achieve purification and concentration in a single step with high yield. This study presents a methodology for optimization of RME with Pseudomonas lipase as model system. Fold-purification, percent recovery and extraction time were the objective functions while the type and concentration of surfactant, contact time, pH, ionic strength, and the ratio of organic to aqueous phase were the decision variables. Under optimized conditions, the AOT (Aerosol OT (bis 2-ethylhexyl) sodium sulfosuccinate)-isooctane system gave a 15-fold purification, 80% recovery and 2.5-fold concentration of the Pseudomonas lipase with process time of 45 min. (C) 2011 Elsevier Ltd. All rights reserved.

10a(Hexadicetyltrimethylammoniumbromide)10aAOT10aCTAB10aLipase10aRME1 aGaikaiwari, Raghavendra, P.1 aWagh, Shilpa, A.1 aKulkarni, Bhaskar, D. uhttps://library.ncl.res.in/content/efficient-lipase-purification-using-reverse-micellar-extraction-002242nas a2200205 4500008004100000022001400041245006900055210006900124260007300193300001200266490000700278520151900285653000901804653003201813653001201845100003201857700002101889700002601910856010001936 2012 eng d a1383-586600aExtraction and purification of tannase by reverse micelle system0 aExtraction and purification of tannase by reverse micelle system aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR a288-2960 v893 a

Tannin acyl hydrolase commonly called as tannase (EC 3.1.1.20) is a commercially important enzyme. Partially purified and concentrated tannase is required for commercial applications. Typical objectives of purification process comprise high fold-purification, recovery and concentration. These objectives may be potentially conflicting. Conventional methods of purification require multiple steps which are time consuming and may cause higher loss. Reverse micellar extraction (RME) using ionic surfactants provides an attractive option for concentration and purification of tannase which is a highly hydrophilic glycoprotein. This study presents an optimized methodology for RME and purification of Aspergillus allahabadi intracellular tannase. Fold-purification, percent recovery and extraction time were the objective while the type and concentration of surfactant, contact time, pH, ionic strength, and the ratio of organic to aqueous phase were the decision variables. Some of these parameters were also studied for their effect on back-extraction. Among the surfactants tested, CTAB-isooctane system was found to be suitable. Under optimized conditions, 12.7-fold purification, 81.2% recovery and 3-fold concentration of tannase with a process time of 45 min was obtained. Conventional purification methods provided a higher fold-purification albeit at a much lower enzyme recovery. Further, the conventional method requires a process time of several hours. (c) 2012 Elsevier B.V. All rights reserved.

10aCTAB10aReverse micellar extraction10aTannase1 aGaikaiwari, Raghavendra, P.1 aWagh, Shilpa, A.1 aKulkarni, Bhaskar, D. uhttps://library.ncl.res.in/content/extraction-and-purification-tannase-reverse-micelle-system-001767nas a2200253 4500008004100000022001400041245011600055210006900171260007400240300001200314490000700326520086700333653001901200653000901219653002201228653003501250653001201285653000801297653000801305100002101313700002401334700002601358856012901384 2012 eng d a0928-070700aSodium-doped lithium zirconate nano squares: synthesis, characterization and applications for CO2 sequestration0 aSodiumdoped lithium zirconate nano squares synthesis characteriz aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB a316-3200 v613 a

Nano squares of sodium-doped lithium zirconate have been synthesized by a simple citrate based sol-gel method at room temperature in the presence of cetyl trimethylammonium bromide (CTAB) as a surfactant. The structural and morphological properties were investigated by X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM) respectively. XRD analysis of sample calcined at 900 A degrees C for 4 h shows the formation of monoclinic lithium zirconate phase. The TEM shows the interesting morphology of formation of nano squares of sodium doped lithium zirconate sample. The carbon dioxide sorption capacity was carried out by TGA analysis at different high temperatures. It is observed that the sample shows similar to 20 wt% carbon dioxide sorption at higher temperature (650 A degrees C) within 20 min which are better than the known reports.

10aCarbon dioxide10aCTAB10aLithium zirconate10aSodium doped lithium zirconate10asol-gel10aTEM10aXRD1 aKhokhani, Mrinal1 aKhomane, Ramdas, B.1 aKulkarni, Bhaskar, D. uhttps://library.ncl.res.in/content/sodium-doped-lithium-zirconate-nano-squares-synthesis-characterization-and-applications-0