02317nas a2200253   4500008004100000022001400041245010300055210006900158260011700227300001000344490000600354520131300360653002701673653002201700653002101722653003501743653003701778653002701815653003001842100001901872700001901891700002101910856013201931       2012                        eng d  a0973-626300aAntibacterial properties of linolenic sophorolipid and its chemically esterified methyl ester form0 aAntibacterial properties of linolenic sophorolipid and its chemi  aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcAUG  a40-450 v73 a<p>The antibacterial activity of Linolenic SL mixture (containing 80% lactone) and its methyl ester derivative against Gram positive (B. subtilis) and Gram negative (E. coli and P. aeruginosa) bacteria is reported here. Bacterial cultures were treated with increasing concentrations of Linolenic SL mixture and its methyl ester derivative and antibacterial activity was checked at different time-intervals (2, 4 and 6 hrs) using standard dilution micromethod and spread plate method. Decrease in bacterial colonies was observed with increase in concentrations of compounds as well as incubation time but the level of effectiveness varies with the compound and bacteria. The minimum inhibitory concentrations (MIC) of Linolenic SL mixture (LNNSL, containing 80% lactone) against B. subtilis, E. coli and P. aeruginosa were found to be 20, 10 and 10 mu g ml(-1) respectively. The MIC values of methyl ester form (LNNSLME) against B. subtilis, E. coli and P. aeruginosa were determined to be &gt;20, 20 and 20 mu g ml(-1) respectively. The results suggest that Linolenic SL mixture (containing 80% lactone) as compared to its methyl ester derivative showed good antibacterial activity towards both the Gram positive and Gram negative bacteria and were found to be more potent against Gram negative bacteria.</p>10aantibacterial activity10aBacillus subtilis10aEscherichia coli10aLinolenic sophorolipid mixture10aMinimum inhibitory concentration10aPseudomonas aeruginosa10aSophorolipid methyl ester1 aReetika, Gupta1 aKumar, Uma, S.1 aAsmita, Prabhune  uhttps://library.ncl.res.in/content/antibacterial-properties-linolenic-sophorolipid-and-its-chemically-esterified-methyl-ester-001731nas a2200229   4500008004100000022001400041245015000055210006900205260007400274300001200348490000700360520079000367653002201157653005301179653004601232653000901278653001901287653003001306100001901336700002101355856012501376       2012                        eng d  a0141-549200aStructural determination and chemical esterification of the sophorolipids produced by Candida bombicola grown on glucose and alpha-linolenic acid0 aStructural determination and chemical esterification of the soph  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcAPR  a701-7070 v343 a<p>The extracellular surface-active glycolipids produced by the yeast, Candida bombicola when grown on glucose and alpha-linolenic acid, were analyzed by HPLC with electro-spray ionization (ESI-MS) and collision-induced dissociation mass spectrometry. The analysis confirmed that the sophorolipid (SL) mixture contained three different forms of C18:3 SL molecules: free acid, lactone and a diacetylated lactone, which has not been reported previously. Also a minor amount of diacetylated lactone form of C18:1 SL was detected. Further, the SL mixture was subjected to chemical esterification reaction with sodium methoxide. The reaction product was analyzed with ESI-MS and confirmed to be the single homogenous esterified product containing C18:3 moieties in its fatty acid chain.</p>10aCandida bombicola10aCollision-induced dissociation mass spectrometry10aElectrospray ionization mass spectrometry10aHPLC10aLinolenic acid10aSophorolipid methyl ester1 aGupta, Reetika1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/structural-determination-and-chemical-esterification-sophorolipids-produced-candida-0