01819nas a2200265 4500008004100000022001400041245011900055210006900174260007300243300001000316490000700326520085300333653002701186653002101213653001901234653002801253653002401281100002001305700002101325700001801346700002201364700002501386700002101411856012101432 2011 eng d a0927-776500aSynthesis of silver nanoparticles in an aqueous suspension of graphene oxide sheets and its antimicrobial activity0 aSynthesis of silver nanoparticles in an aqueous suspension of gr aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR a16-220 v833 a

A solution-based approach to the synthesis of silver (Ag) nanoparticles by chemical reduction of AgNO3 in a graphene oxide (GrO) suspension is demonstrated. X-ray diffraction and transmission electron microscopy indicate that the Ag nanoparticles, of size range 5-25 nm, were decorated on the GrO sheets. The size and shape of the Ag nanoparticles are dependent on the concentration of the AgNO3 solution. Antimicrobial activity of such hybrids materials is investigated against the Gram negative bacteria Escherichia coli and Pseudomonous aeruginosa. The bacterial growth kinetics was monitored in nutrient broth supplemented with the Ag nanoparticle-GrO suspension at different conditions. It was observed that P. aeruginosa is comparatively more sensitive to the Ag nanoparticle-GrO suspension. (C) 2010 Elsevier B.V. All rights reserved.

10aAntimicrobial activity10aEscherichia coli10agraphene oxide10aPseudomonous aeruginosa10aSilver nanoparticle1 aDas, Manash, R.1 aSarma, Rupak, K.1 aSaikia, Ratul1 aKale, Vinayak, S.1 aShelke, Manjusha, V.1 aSengupta, Pinaki uhttps://library.ncl.res.in/content/synthesis-silver-nanoparticles-aqueous-suspension-graphene-oxide-sheets-and-its-002317nas a2200253 4500008004100000022001400041245010300055210006900158260011700227300001000344490000600354520131300360653002701673653002201700653002101722653003501743653003701778653002701815653003001842100001901872700001901891700002101910856013201931 2012 eng d a0973-626300aAntibacterial properties of linolenic sophorolipid and its chemically esterified methyl ester form0 aAntibacterial properties of linolenic sophorolipid and its chemi aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcAUG a40-450 v73 a

The antibacterial activity of Linolenic SL mixture (containing 80% lactone) and its methyl ester derivative against Gram positive (B. subtilis) and Gram negative (E. coli and P. aeruginosa) bacteria is reported here. Bacterial cultures were treated with increasing concentrations of Linolenic SL mixture and its methyl ester derivative and antibacterial activity was checked at different time-intervals (2, 4 and 6 hrs) using standard dilution micromethod and spread plate method. Decrease in bacterial colonies was observed with increase in concentrations of compounds as well as incubation time but the level of effectiveness varies with the compound and bacteria. The minimum inhibitory concentrations (MIC) of Linolenic SL mixture (LNNSL, containing 80% lactone) against B. subtilis, E. coli and P. aeruginosa were found to be 20, 10 and 10 mu g ml(-1) respectively. The MIC values of methyl ester form (LNNSLME) against B. subtilis, E. coli and P. aeruginosa were determined to be >20, 20 and 20 mu g ml(-1) respectively. The results suggest that Linolenic SL mixture (containing 80% lactone) as compared to its methyl ester derivative showed good antibacterial activity towards both the Gram positive and Gram negative bacteria and were found to be more potent against Gram negative bacteria.

10aantibacterial activity10aBacillus subtilis10aEscherichia coli10aLinolenic sophorolipid mixture10aMinimum inhibitory concentration10aPseudomonas aeruginosa10aSophorolipid methyl ester1 aReetika, Gupta1 aKumar, Uma, S.1 aAsmita, Prabhune uhttps://library.ncl.res.in/content/antibacterial-properties-linolenic-sophorolipid-and-its-chemically-esterified-methyl-ester-002223nas a2200289 4500008004100000245012600041210006900167260000800236300000900244490000700253520117200260653001301432653001301445653002001458653002101478653002601499653002601525100003801551700004201589700002301631700002801654700002701682700002701709700003701736700003401773856012601807 2020 eng d00aBacterial biofilm formation using PCL/curcumin electrospun fibers and its potential use for biotechnological applications0 aBacterial biofilm formation using PCLcurcumin electrospun fibers cDEC a55560 v133 a

Electrospun nanofibers are used for many applications due to their large surface area, mechanical properties, and bioactivity. Bacterial biofilms are the cause of numerous problems in biomedical devices and in the food industry. On the other hand, these bacterial biofilms can produce interesting metabolites. Hence, the objective of this study is to evaluate the efficiency of poly (x190;- caprolactone)/Curcumin (PCL/CUR) nanofibers to promote bacterial biofilm formation. These scaffolds were characterized by scanning electron microscopy (SEM), which showed homogeneous fibers with diameters between 441-557 nm; thermogravimetric analysis and differential scanning calorimetry (TGA and DSC) demonstrated high temperature resilience with degradation temperatures over >350 degrees C; FTIR and H-1-NMR serve as evidence of CUR incorporation in the PCL fibers. PCL/CUR scaffolds successfully promoted the formation of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa biofilms. These results will be valuable in the study of controlled harvesting of pathogenic biofilms as well as in metabolites production for biotechnological purposes.

10aBacteria10acurcumin10aelectrospinning10aEscherichia coli10aPseudomona aeruginosa10aStaphylococcus aureus1 aPompa-Monroy, Daniella, Alejandra1 aFigueroa-Marchant, Paulina, Guadalupe1 aDastager, Syed, G.1 aThorat, Meghana, Namdeo1 aIglesias, Ana, Leticia1 aMiranda-Soto, Valentin1 aPerez-Gonzalez, Graciela, Lizeth1 aVillarreal-Gomez, Luis, Jesus uhttps://library.ncl.res.in/content/bacterial-biofilm-formation-using-pclcurcumin-electrospun-fibers-and-its-potential-use02164nas a2200229 4500008004100000022001400041245012300055210006900178260000800247300001200255490000800267520134900275653002101624653001601645653002601661653002901687653002201716100002401738700002301762700002301785856012601808 2021 eng d a0175-759800aMetabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae0 aMetabolic engineering and synthetic biology for isoprenoid produ cJAN a457-4750 v1053 a

Isoprenoids, often called terpenoids, are the most abundant and highly diverse family of natural organic compounds. In plants, they play a distinct role in the form of photosynthetic pigments, hormones, electron carrier, structural components of membrane, and defence. Many isoprenoids have useful applications in the pharmaceutical, nutraceutical, and chemical industries. They are synthesized by various isoprenoid synthase enzymes by several consecutive steps. Recent advancement in metabolic engineering and synthetic biology has enabled the production of these isoprenoids in the heterologous host systems like Escherichia coli and Saccharomyces cerevisiae. Both heterologous systems have been engineered for large-scale production of value-added isoprenoids. This review article will provide the detailed description of various approaches used for engineering of methyl-d-erythritol-4-phosphate (MEP) and mevalonate (MVA) pathway for synthesizing isoprene units (C-5) and ultimate production of diverse isoprenoids. The review particularly highlighted the efforts taken for the production of C-5-C-20 isoprenoids by metabolic engineering techniques in E. coli and S. cerevisiae over a decade. The challenges and strategies are also discussed in detail for scale-up and engineering of isoprenoids in the heterologous host systems.

10aEscherichia coli10aIsoprenoids10aMetabolic engineering10aSaccharomyces cerevisiae10asynthetic biology1 aNavale, Govinda, R.1 aDharne, Mahesh, S.1 aShinde, Sandip, S. uhttps://library.ncl.res.in/content/metabolic-engineering-and-synthetic-biology-isoprenoid-production-escherichia-coli-and