02494nas a2200241   4500008004100000022001400041245009200055210006900147260009500216300001200311490000600323520159800329653002001927653002101947653003001968653002801998653002202026100001802048700002302066700002802089700001802117856011702135       2009                        eng d  a1941-490000aEudragit (R) EPO based nanoparticle suspension of andrographolide: in vitro and in vivo0 aEudragit R EPO based nanoparticle suspension of andrographolide   a25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USAbAMER SCIENTIFIC PUBLISHERScDEC  a156-1640 v13 a<p>Characteristic formulation of an isolated active phytoconstituent is of great therapeutic significance. The purpose of this work was to develop Eudragit (R) EPO based nanoparticle suspension of isolated andrographolide in order to increase its solubility and efficacy. Andrographolide was isolated from Andrographis paniculata and Its Nanoparticle suspension was prepared by precipitation technique. The 3(2) factorial design was used to study the effect of Eudragit (R) EPO and Pluronic (R) F-68 on characteristics of nanoparticle suspension. The optimized formulation was subjected to lyophilization. The nanoparticle suspension, lyophilized suspension and the redispersed lyophilized nanoparticle suspension were characterized by drug content, encapsulation efficiency, particle size, electrokinetic properties, DSC, PXRD, FTIR, TEM and in vitro drug release. Optimized batch showed particle size 255 +/- 9 nm with encapsulation efficiency 93.8 +/- 0.67% and zeta potential 29.3 +/- 3.4 mV. There was marked increase in drug dissolution with complete drug release within 10 minutes in nanoparticle suspension and redispersed naoparticle suspension as compared to pure drug. However, lyophilization retarded the drug release. The nanoparticle suspension and redispersed nanoparticle suspension also showed improved hepatoprotectivity as compared to andrographolide against CCl(4)-induced hepatotoxicity in rats. The results were confirmed by histopathological studies on hepatic lesions. Thus particle engineering can be used to improve pharmaceutical properties of phytoconstituents.</p>10aAndrographolide10aEudragit (R) EPO10aHepatoprotective Activity10aNanoparticle Suspension10aPluronic (R) F-681 aBothiraja, C.1 aPawar, Atmaram, P.1 aShaikh, Karimunnisa, S.1 aSher, Praveen  uhttps://library.ncl.res.in/content/eudragit-r-epo-based-nanoparticle-suspension-andrographolide-vitro-and-vivo-002321nas a2200289   4500008004100000022001400041245013600055210006900191260011100260300001200371490000700383520126500390653002001655653003401675653001401709653001601723653001601739100001401755700001801769700001501787700001401802700002201816700002601838700002301864700001801887856012601905       2012                        eng d  a1566-524000aAndrographolide inhibits osteopontin expression and breast tumor growth through down regulation of PI3 Kinase/Akt signaling pathway0 aAndrographolide inhibits osteopontin expression and breast tumor  aEXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATESbBENTHAM SCIENCE PUBL LTDcSEP  a952-9660 v123 a<p>Breast cancer is one of the most common cancers among women in India and around the world. Despite recent advancement in the treatment of breast cancer, the results of chemotherapy to date remain unsatisfactory, prompting a need to identify natural agents that could target cancer efficiently with least side effects. Andrographolide (Andro) is one such molecule which has been shown to possess inhibitory effect on cancer cell growth. In this study, Andro, a natural diterpenoid lactone isolated from Andrographis paniculata has been shown to inhibit breast cancer cell proliferation, migration and arrest cell cycle at G2/M phase and induces apoptosis through caspase independent pathway. Our experimental evidences suggest that Andro attenuates endothelial cell motility and tumor-endothelial cell interaction. Moreover, Andro suppresses breast tumor growth in orthotopic NOD/SCID mice model. The anti-tumor activity of Andro in both in vitro and in vivo model was correlated with down regulation of PI3 kinase/Akt activation and inhibition of pro-angiogenic molecules such as OPN and VEGF expressions. Collectively, these results demonstrate that Andro may act as an effective anti-tumor and anti-angiogenic agent for the treatment of breast cancer.</p>10aAndrographolide10aangiogenesis and breast tumor10amigration10aosteopontin10aPI 3 kinase1 aKumar, S.1 aPatil, H., S.1 aSharma, P.1 aKumar, D.1 aDasari, Sreekanth1 aPuranik, Vedavati, G.1 aThulasiram, H., V.1 aKundu, G., C.  uhttps://library.ncl.res.in/content/andrographolide-inhibits-osteopontin-expression-and-breast-tumor-growth-through-down-001927nas a2200289   4500008004100000022001400041245009800055210006900153260000900222300001400231490000700245520098200252653001401234653002001248653002701268653001701295653001401312653001101326653002001337100002301357700002301380700001501403700002201418700003001440700003701470856013001507       2018                        eng d  a0960-894X00aRegioselective and efficient enzymatic synthesis of antimicrobial andrographolide derivatives0 aRegioselective and efficient enzymatic synthesis of antimicrobia  cAPR   a1132-11370 v283 a<p>Labdane diterpene andrographolide (1) is a major constituent of Andrographis paniculata and known to exhibit wide spectrum of biological activities. In this study, regioselective monoesters of (1) have been synthesized by using Amano lipase AK (Pseudomonas fluorescens) as a biocatalyst. Amano lipase AK was able to execute highly efficient esterification of hydroxyl group attached to C-14 carbon of (1) in presence of acyl donors. Among the various synthesized derivatives including two novel compounds such as andrographolide-14-propionate (3) and andrographolide-14-caproate (5) displayed antimicrobial activity against Staphylococcus aureus with low minimal inhibitory concentration (MIC) 4 mu g/mL and 16 mu g/mL respectively. Furthermore, they have shown low hemolysis activity at their respective MIC and increase in the permeability of the bacterial cell membrane as delineated by FITC uptake and SEM imaging studies. (C) 2018 Elsevier Ltd. All rights reserved.</p>10aAcylation10aAndrographolide10aAntimicrobial activity10abiocatalysis10aHemolysis10aLipase10aNatural product1 aPatil, Harshal, S.1 aJadhav, Dipesh, D.1 aPaul, Ajay1 aMulani, Fayaj, A.1 aKaregaonkar, Shrikant, J.1 aThulasiram, Hirekodathakallu, V.  uhttps://library.ncl.res.in/content/regioselective-and-efficient-enzymatic-synthesis-antimicrobial-andrographolide-derivatives