01995nas a2200229 4500008004100000022001400041245009000055210006900145260008700214300001200301490000700313520115800320653002401478653001501502653002901517653002701546653001601573100001601589700002001605700002101625856011901646 2009 eng d a0963-996900aHypocholesteremic effect of bile salt hydrolase from Lactobacillus buchneri ATCC 40050 aHypocholesteremic effect of bile salt hydrolase from Lactobacill aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbICBF Forum; Biotech Res Soc IndiacMAY a516-5200 v423 a

The effect of oral administration of the immobilized bile salt hydrolase enzyme on serum cholesterol. triglyceride, high density lipoprotein levels and its application in the therapeutic treatment of hypercholesteremia was assessed. Culture conditions were optimized for the production of bile salt hydrolase, which resulted in 2.9-fold enhancement in activity. Bile salt hydrolase (BSH; E.C.3.5.1.24) was isolated from Lactobacillus buchneri ATCC 4005 and immobilized in 0.5% gellan gum gel. The immobilized enzyme was orally delivered in wistar rats, induced with hypercholesteremia by triton X-100. The serum cholesterol and triglycerides were reduced by 50% and 15%, respectively, in the group fed with immobilized enzyme 10 IU/kg dose whereas administration of 20 IU/kg immobilized enzyme resulted in reduction of serum cholesterol by 58% and triglycerides by 45%, respectively. The results indicate that bile salt hydrolase has potential cholesterol lowering property and oral administration of the immobilized enzyme is an alternative pharmacological approach to reduce serum cholesterol levels. (C) 2009 Elsevier Ltd. All rights reserved.

10aBile salt hydrolase10aGellan gum10aHypocholesteremic effect10aLactobacillus buchneri10aWistar Rats1 aSridevi, N.1 aVishwe, Pradnya1 aPrabhune, Asmita uhttps://library.ncl.res.in/content/hypocholesteremic-effect-bile-salt-hydrolase-lactobacillus-buchneri-atcc-4005-002299nas a2200265 4500008004100000022001400041245010800055210006900163260007600232300001200308490000700320520135100327653001501678653002001693653001501713653002301728653002201751100001801773700001701791700002701808700001901835700003001854700002201884856012701906 2011 eng d a0260-437X00aBiocompatible gellan gum-reduced gold nanoparticles: cellular uptake and subacute oral toxicity studies0 aBiocompatible gellan gumreduced gold nanoparticles cellular upta aCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USAbWILEY-BLACKWELLcJUL a411-4200 v313 a

Currently gold nanoparticles are being explored for drug delivery and other biomedical applications; therefore it is necessary to study the fate of such nanoparticles inside the body. The objective of the present study was to investigate the cellular uptake and toxicity of the gold nanoparticles synthesized using a microbial polysaccharide, gellan gum, as a capping and reducing agent. The cellular uptake of gold nanoparticles was studied on mouse embryonic fibroblast cells, NIH3T3 and human glioma cell line, LN-229. The cellular uptake study indicated that the gellan gum-reduced gold nanoparticles were located in cancer cells (LN-229) while no uptake was observed in normal mouse embryonic fibroblast cells (NIH3T3). The toxicity of the gold nanoparticles was evaluated by carrying out subacute 28 day oral toxicity studies in rats. Subacute administration of gum-reduced gold nanoparticles to the rats did not show any hematological or biochemical abnormalities. The weight and normal architecture of various organs did not change compared with control. The current findings, while establishing the specific uptake of nanoparticles into cancerous cells, also demonstrates that the gellan gum-reduced gold nanoparticles are devoid of toxicity in animals following oral administration. Copyright (c) 2010 John Wiley & Sons, Ltd.

10acell lines10acellular uptake10aGellan gum10agold nanoparticles10asubacute toxicity1 aDhar, Sheetal1 aMali, Vishal1 aBodhankar, Subhash, L.1 aShiras, Anjali1 aPrasad, Bhagavatula L. V.1 aPokharkar, Varsha uhttps://library.ncl.res.in/content/biocompatible-gellan-gum-reduced-gold-nanoparticles-cellular-uptake-and-subacute-oral-002008nas a2200229 4500008004100000022001400041245016100055210006900216260011700285300001000402490000600412520109600418653001501514653001901529653001901548653001401567653001301581653001601594100002401610700002101634856012301655 2012 eng d a0973-626300aGellan Gum a novel polysaccharide matrix for immobilization of thermo-tolerant yeast cells with invertase activity: factorial design and rheological studies0 aGellan Gum a novel polysaccharide matrix for immobilization of t aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcNOV a81-870 v73 a

The microbial extracellular polysaccharide gellan gum has been explored for its application in whole cell immobilization. Preliminary evaluation of the physicochemical properties of gellan gum in the presence of monovalent cation tetramethyl ammonium chloride (TMACl) was carried out. Gellan gum immobilized cells demonstrated better rheological properties than gellan gum. Attempts have been made to reveal the evaluation of gellan gum as a matrix to immobilize a thermotolerant yeast Kluyveromyces marxianus NCYC 2675. A 3(2) factorial design was used to study the simultaneous effect of two variables. The effect of the polymer concentration and TMACl concentration on various dependent variables like gelling temperature, mean particle size and enzyme activity of the yeast cells was studied. A statistical model with a significant interaction term was obtained to predict the results. Further, optimized immobilized system was evaluated with significant storage stability at 10 C and enhanced reusability with maximum conversion of > 90 % when crosslinked with glutaraldehyde.

10aGellan gum10aGlutaraldehyde10aImmobilization10ainvertase10aRheology10ayeast cells1 aSulabha, Karandikar1 aAsmita, Prabhune uhttps://library.ncl.res.in/content/gellan-gum-novel-polysaccharide-matrix-immobilization-thermo-tolerant-yeast-cells-0