01730nas a2200241   4500008004100000022001400041245010200055210006900157260007400226300001200300490000700312520082700319653001601146653001301162653004101175653003401216653001701250653002101267653003301288100002001321700001901341856012801360       2012                        eng d  a0920-906900aEffect of addition of `carrier' DNA during transient protein expression in suspension CHO culture0 aEffect of addition of carrier DNA during transient protein expre  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcDEC  a613-6220 v643 a<p>Transient protein expression using polyethyleneimine as a transfection agent is useful for the rapid production of small amounts of recombinant proteins. It is known that an increase in extracellular DNA concentration during transfection can lead to a nonlinear increase in intracellular DNA concentration. We present an approach that hypothesizes that this nonlinearity can be used to decrease the amount of plasmid required for productive transfections. Through addition of non coding `carrier' DNA to increase total DNA concentration during transfection, we report a statistically significant increase in protein (IgG) expression per unit plasmid used for transfection. This approach could be useful to increase protein yields for large scale transfections under conditions where plasmid availability is limited.</p>10aCarrier DNA10aCell age10aDNA: polyethyleneimine particle size10aMedium equilibration with CO210apH excursion10aSalmon sperm DNA10aTransient protein expression1 aPradhan, Ketaki1 aGadgil, Mugdha  uhttps://library.ncl.res.in/content/effect-addition-carrier-dna-during-transient-protein-expression-suspension-cho-culture-0